Reviewed, UniProtKB/Swiss-Prot P29147 (BDH_RAT)

Names and origin

Protein names

Recommended name:
    D-beta-hydroxybutyrate dehydrogenase, mitochondrial
      Short name=BDH
    EC=1.1.1.30
Alternative name(s):
    3-hydroxybutyrate dehydrogenase

Gene names

Name:	Bdh1
Synonyms:	Bdh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH.
Enzyme regulation	Requires phosphatidylcholine as an allosteric activator for enzymatic activity By similarity.
Subunit structure	Homotetramer.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Allosteric enzyme Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxybutyrate dehydrogenase activity Ref.1 Traceable author statement. Source: RGD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Mitochondrion
	Chain	47 – 343	297	D-beta-hydroxybutyrate dehydrogenase, mitochondrial		PRO_0000031962
Regions
	Nucleotide binding	59 – 83	25	NAD By similarity
Sites
	Active site	208	1	Proton acceptor By similarity
	Binding site	195	1	Substrate By similarity
Amino acid modifications
	Modified residue	132	1	N6-acetyllysine By similarity
	Modified residue	275	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	90 – 91	2	DK → EQ in AAB59684. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29147-1 [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 6903D2ED27F2B055	FASTA	343	38,202
	10 20 30 40 50 60 MLAARLSRPL SQLPGKALSV CDRENGTRHT LLFYPASFSP DTRRTYTSQA DAASGKAVLV 70 80 90 100 110 120 TGCDSGFGFS LAKHLHSKGF LVFAGCLLKD KGDAGVRELD SLKSDRLRTI QLNVCNSEEV 130 140 150 160 170 180 EKAVETVRSG LKDPEKGMWG LVNNAGISTF GEVEFTSMET YKEVAEVNLW GTVRTTKSFL 190 200 210 220 230 240 PLLRRAKGRV VNISSMLGRM ANPARSPYCI TKFGVEAFSD CLRYEMHPLG VKVSVVEPGN 250 260 270 280 290 300 FIAATSLYSP ERIQAIAKKM WDELPEVVRK DYGKKYFDEK IAKMETYCNS GSTDTSSVIN 310 320 330 340 AVTHALTAAT PYTRYHPMDY YWWLRMQVMT HFPGAISDKI YIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of rat liver D-beta-hydroxybutyrate dehydrogenase from cDNA and protein analyses: a short-chain alcohol dehydrogenase." Churchill P., Hempel J., Romovacek H., Zhang W.W., Churchill S., Brennan M. Biochemistry 31:3793-3799(1992) [PubMed: 1567834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M89902 mRNA. Translation: AAB59684.1. Different initiation. BC085916 mRNA. Translation: AAH85916.1. Different initiation.
IPI	IPI00480620.
PIR	A42345.
RefSeq	NP_446447.2.
UniGene	Rn.36635
3D structure databases
HSSP	HSSP built from PDB template 1FDS based on UniProtKB P14061.
ModBase	Search...
Protein-protein interaction databases
STRING	P29147.
Genome annotation databases
Ensembl	ENSRNOT00000002366; ENSRNOP00000002366; ENSRNOG00000001736; Rattus norvegicus. [Genome view]
GeneID	117099.
KEGG	rno:117099.
NMPDR	fig|10116.3.peg.7406.
UCSC	BC085916. rat.
Organism-specific databases
CTD	117099.
RGD	620131. Bdh1.
Phylogenomic databases
HOVERGEN	P29147.
OMA	SVTECIV.
Enzyme and pathway databases
BRENDA	1.1.1.30. 248.
Gene expression databases
ArrayExpress	P29147.
Genevestigator	P29147.
GermOnline	ENSRNOG00000001736. Rattus norvegicus.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	619980.

Entry information

Entry name

BDH_RAT

Accession

Primary (citable) accession number: P29147
Secondary accession number(s): Q5U2Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 5, 2005
Last modified:	November 3, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)