Reviewed, UniProtKB/Swiss-Prot P29115 (CHI1_STROI)

Names and origin

Protein names	Recommended name:     Chitinases 70, 30, and 20.5 kDa     EC=3.2.1.14
Organism	Streptomyces olivaceoviridis (Streptomyces corchorusii)
Taxonomic identifier	1921 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	74 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Able to cleave chitin oligomers from N=3 to 6.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subunit structure	Homodimer, but homotrimers and homotetramers could be observed for the 20.5 and 30 kDa chitinases.
Post-translational modification	The 70 kDa chitinase is probably the precursor protein of the 30 and 20.5 kDa chitinases.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›74	›74	Chitinases 70, 30, and 20.5 kDa		PRO_0000077045
Regions
	Region	1 – 27	27	N-terminus of 70 kDa chitinase
	Region	28 – 52	25	N-terminus of 30 kDa chitinase
	Region	53 – 74	22	N-terminus of 20.5 kDa chitinase
Experimental info
	Non-adjacent residues	27 – 28	2
	Non-adjacent residues	52 – 53	2
	Non-terminal residue	74	1

Sequences

	Sequence		Length	Mass (Da)
	P29115-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 33084754CC5E7552	FASTA	74	8,102
	10 20 30 40 50 60 XTSATATYAK TQDWGSCFEG KWTIKNTAAC SSYPSWVAGR SYAAGDIVYY TDXGYTDLPV 70 SRQKMCQNGM VTNC

References

[1]	"Chitinases of Streptomyces olivaceoviridis and significance of processing for multiplicity." Romaguera A., Menge U., Breves R., Diekmann H. J. Bacteriol. 174:3450-3454(1992) [PubMed: 1592803] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: ATCC 11238 / DSM 41433 / NCIB 8592 / QM B814.

Cross-references

Sequence databases
PIR	A44908. B44908. D44908.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.14. 119250.
Family and domain databases
InterPro	IPR001919. CBD_bac. IPR001579. Glyco_hydro_18_chit_AS. [Graphical view]
Pfam	PF00553. CBM_2. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI1_STROI

Accession

Primary (citable) accession number: P29115

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	March 24, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)