Reviewed, UniProtKB/Swiss-Prot P29114 (LOX1_HORVU)

Names and origin

Protein names

Recommended name:
    Lipoxygenase 1
    EC=1.13.11.12

Gene names

Name:	LOX1.1
Synonyms:	LOXA

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
Catalytic activity	Linoleate + O2 = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer.
Developmental stage	In both quiescent and germinating seeds.
Miscellaneous	With linoleate as substrate, lipoxygenase 1 shows a specificity for carbon 9 as the site for hydroperoxidation (in contrast to lipoxygenase 2, which shows a preference for carbon 13).
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 862	862	Lipoxygenase 1		PRO_0000220723
Regions
	Domain	34 – 161	128	PLAT
	Domain	164 – 862	699	Lipoxygenase
Sites
	Metal binding	517	1	Iron; catalytic By similarity
	Metal binding	522	1	Iron; catalytic By similarity
	Metal binding	708	1	Iron; catalytic By similarity
	Metal binding	712	1	Iron; catalytic By similarity
	Metal binding	862	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29114-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: F55954473467BEAA	FASTA	862	96,393
	10 20 30 40 50 60 MLLGGLIDTL TGANKSARLK GTVVLMRKNV LDLNDFGATI IDGIGEFLGK GVTCQLISST 70 80 90 100 110 120 AVDQDNGGRG KVGAEAELEQ WVTSLPSLTT GESKFGLTFD WEVEKLGVPG AIVVNNYHSS 130 140 150 160 170 180 EFLLKTITLH DVPGRSGNLT FVANSWIYPA ANYRYSRVFF ANDTYLPSQM PAALKPYRDD 190 200 210 220 230 240 ELRNLRGDDQ QGPYQEHDRI YRYDVYNDLG EGRPILGGNS DHPYPRRGRT ERKPNASDPS 250 260 270 280 290 300 LESRLSLLEQ IYVPRDEKFG HLKTSDFLGY SIKAITQGIL PAVRTYVDTT PGEFDSFQDI 310 320 330 340 350 360 INLYEGGIKL PKVAALEELR KQFPLQLIKD LLPVGGDSLL KLPVPHIIQE NKQAWRTDEE 370 380 390 400 410 420 FAREVLAGVN PVMITRLTEF PPKSSLDPSK FGDHTSTITA EHIEKNLEGL TVQQALESNR 430 440 450 460 470 480 LYILDHHDRF MPFLIDVNNL PGNFIYATRT LFFLRGDGRL TPLAIELSEP IIQGGLTTAK 490 500 510 520 530 540 SKVYTPVPSG SVEGWVWELA KAYVAVNDSG WHQLVSHWLN THAVMEPFVI STNRHLSVTH 550 560 570 580 590 600 PVHKLLSPHY RDTMTINALA RQTLINAGGI FEMTVFPGKF ALGMSAVVYK DWKFTEQGLP 610 620 630 640 650 660 DDLIKRGMAV EDPSSPYKVR LLVSDYPYAA DGLAIWHAIE QYVSEYLAIY YPNDGVLQGD 670 680 690 700 710 720 TEVQAWWKET REVGHGDLKD APWWPKMQSV PELAKACTTI IWIGSALHAA VNFGQYPYAG 730 740 750 760 770 780 FLPNRPTVSR RRMPEPGTEE YAELERDPER AFIHTITSQI QTIIGVSLLE VLSKHSSDEL 790 800 810 820 830 840 YLGQRDTPEW TSDPKALEVF KRFSDRLVEI ESKVVGMNHD PELKNRNGPA KFPYMLLYPN 850 860 TSDHKGAAAG LTAKGIPNSI SI

References

[1]	"Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence." van Mechelen J.R., Smits M., Douma A.C., Rouster J., Cameron-Mills V., Heidekamp F., Valk B.E. Biochim. Biophys. Acta 1254:221-225(1995) [PubMed: 7827128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Triumph.
[2]	"Differential defense reactions in leaf tissues of barley in response to infection by Rhynchosporium secalis and to treatment with a fungal avirulence gene product." Steiner-Lange S., Fischer A., Boettcher A., Rouhara I., Liedgens H., Schmelzer E., Knogge W. Mol. Plant Microbe Interact. 16:893-902(2003) [PubMed: 14558691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 512-862.
[3]	"Purification and characterization of two lipoxygenase isoenzymes from germinating barley." Doderer A., Kokkelink I., van der Veen S., Valk B.E., Schram A.W., Douma A.C. Biochim. Biophys. Acta 1120:97-104(1992) [PubMed: 1554746] [Abstract] Cited for: PROTEIN SEQUENCE OF 274-294 AND 832-845. Strain: cv. Triumph. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L35931 Genomic DNA. Translation: AAA64893.1. AY220737 mRNA. Translation: AAP04432.1. Different initiation.
PIR	S21772. S22236. T05941.
UniGene	Hv.9141
3D structure databases
HSSP	HSSP built from PDB template 1FGT based on UniProtKB P08170.
ModBase	Search...
Organism-specific databases
Gramene	P29114.
Enzyme and pathway databases
BRENDA	1.13.11.12. 283.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX1_HORVU

Accession

Primary (citable) accession number: P29114
Secondary accession number(s): Q42845, Q84QC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)