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Protein

Ketol-acid reductoisomerase

Gene

ilvC

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.

Pathway: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit (ilvH)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Probable branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathway: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit (ilvH)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Probable branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081Sequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00047; UER00056.
UPA00049; UER00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (EC:1.1.1.86)
Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacyl reductoisomerase
Gene namesi
Name:ilvC
Ordered Locus Names:sll1363
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 331330Ketol-acid reductoisomerasePRO_0000151372Add
BLAST

Proteomic databases

PaxDbiP29107.

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

DIPiDIP-48805N.
IntActiP29107. 1 interaction.
STRINGi1148.SYNGTS_2962.

Structurei

3D structure databases

ProteinModelPortaliP29107.
SMRiP29107. Positions 3-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.Curated

Phylogenomic databases

eggNOGiCOG0059.
HOGENOMiHOG000016230.
InParanoidiP29107.
KOiK00053.
OMAiVYYDKDI.
OrthoDBiEOG625K07.
PhylomeDBiP29107.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
HAMAPiMF_00435. IlvC.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR013023. AcH_isomrdctse.
IPR000506. AcH_isomrdctse_C.
IPR013116. IlvN.
[Graphical view]
PANTHERiPTHR21371. PTHR21371. 1 hit.
PfamiPF01450. IlvC. 1 hit.
PF07991. IlvN. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00465. ilvC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARMYYDQDA NLDLLAGKTV AIIGYGSQGH AHALNLKDSG VNVVVGLYSG
60 70 80 90 100
SKSVAKAEGA GLKVLSVAEA AKAADLIMIL LPDEVQKTVY EAEIAPNLVA
110 120 130 140 150
GNVLLFAHGF NINFAQIVPP ADVDVVMAAP KGPGHLVRRT YEQGQGVPAL
160 170 180 190 200
FAVYQDASGQ ARDYAMAYAK GIGGTRAGIL ETTFREETET DLFGEQVVLC
210 220 230 240 250
GGLTALIKAG FDTLVEAGYQ PELAYFECLH EVKLIVDLIV EGGLAKMRDS
260 270 280 290 300
ISNTAEYGDL TRGPRIVTEE TKAEMRQILD EIQSGQFARE FVLENQAGKP
310 320 330
GFTAMRRRES EELIEEVGKD LRAMFSWLKD R
Length:331
Mass (Da):35,822
Last modified:January 23, 2007 - v3
Checksum:i070C9D494F39CD44
GO

Sequence cautioni

The sequence BAA18666.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18666.1. Different initiation.
PIRiS76754. A47037.
RefSeqiYP_007452730.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA18666; BAA18666; BAA18666.
KEGGisyn:sll1363.
PATRICi23843490. VBISynSp132158_3280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18666.1. Different initiation.
PIRiS76754. A47037.
RefSeqiYP_007452730.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP29107.
SMRiP29107. Positions 3-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48805N.
IntActiP29107. 1 interaction.
STRINGi1148.SYNGTS_2962.

Proteomic databases

PaxDbiP29107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18666; BAA18666; BAA18666.
KEGGisyn:sll1363.
PATRICi23843490. VBISynSp132158_3280.

Phylogenomic databases

eggNOGiCOG0059.
HOGENOMiHOG000016230.
InParanoidiP29107.
KOiK00053.
OMAiVYYDKDI.
OrthoDBiEOG625K07.
PhylomeDBiP29107.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00056.
UPA00049; UER00060.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
HAMAPiMF_00435. IlvC.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR013023. AcH_isomrdctse.
IPR000506. AcH_isomrdctse_C.
IPR013116. IlvN.
[Graphical view]
PANTHERiPTHR21371. PTHR21371. 1 hit.
PfamiPF01450. IlvC. 1 hit.
PF07991. IlvN. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00465. ilvC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  2. "Purification of glutamyl-tRNA reductase from Synechocystis sp. PCC 6803."
    Rieble S., Beale S.I.
    J. Biol. Chem. 266:9740-9745(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-43.

Entry informationi

Entry nameiILVC_SYNY3
AccessioniPrimary (citable) accession number: P29107
Secondary accession number(s): P74559
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally isolated as a glutamyl-tRNA reductase.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.