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P29094

- O16G_GEOTM

UniProt

P29094 - O16G_GEOTM

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Protein

Oligo-1,6-glucosidase

Gene
malL
Organism
Geobacillus thermoglucosidasius (Bacillus thermoglucosidasius)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Temperature dependencei

Extremely thermostable.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991Nucleophile By similarity
Active sitei256 – 2561Proton donor By similarity
Sitei330 – 3301Transition state stabilizer By similarity

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. oligo-1,6-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase (EC:3.2.1.10)
Alternative name(s):
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase
Sucrase-isomaltase
Short name:
Isomaltase
Gene namesi
Name:malL
OrganismiGeobacillus thermoglucosidasius (Bacillus thermoglucosidasius)
Taxonomic identifieri1426 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Oligo-1,6-glucosidasePRO_0000054318Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP29094.
SMRiP29094. Positions 1-560.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P29094-1 [UniParc]FASTAAdd to Basket

« Hide

MERVWWKEAV VYQIYPRSFY DSNGDGIGDI RGIIAKLDYL KELGVDVVWL    50
SPVYKSPNDD NGYDISDYRD IMDEFGTMAD WKTMLEEMHK RGIKLVMDLV 100
VNHTSDEHPW FIESRKSKDN PYRDYYIWRP GKNGKEPNNW ESVFSGSAWE 150
YDEMTGEYYL HLFSKKQPDL NWENPKVRRE VYEMMKFWLD KGVDGFRMDV 200
INMISKVPEL PDGEPQSGKK YASGSRYYMN GPRVHEFLQE MNREVLSKYD 250
IMTVGETPGV TPKEGILYTD PSRRELNMVF QFEHMDLDSG PGGKWDIRPW 300
SLADLKKTMT KWQKELEGKG WNSLYLNNHD QPRAVSRFGD DGKYRVESAK 350
MLATFLHMMQ GTPYIYQGEE IGMTNVRFPS IEDYRDIETL NMYKERVEEY 400
GEDPQEVMEK IYYKGRDNAR TPMQWDDSEN AGFTAGTPWI PVNPNYKEIN 450
VKAALEDPNS VFHYYKKLIQ LRKQHDIIVY GTYDLILEDD PYIYRYTRTL 500
GNEQLIVITN FSEKTPVFRL PDHIIYKTKE LLISNYDVDE AEELKEIRLR 550
PWEARVYKIR LP 562
Length:562
Mass (Da):66,505
Last modified:December 1, 1992 - v1
Checksum:i925EB5924726D42A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10487 Genomic DNA. Translation: BAA01368.1.
PIRiA41707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10487 Genomic DNA. Translation: BAA01368.1 .
PIRi A41707.

3D structure databases

ProteinModelPortali P29094.
SMRi P29094. Positions 1-560.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Proline residues responsible for thermostability occur with high frequency in the loop regions of an extremely thermostable oligo-1,6-glucosidase from Bacillus thermoglucosidasius KP1006."
    Watanabe K., Chishiro K., Kitamura K., Suzuki Y.
    J. Biol. Chem. 266:24287-24294(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12, TEMPERATURE DEPENDENCE.
    Strain: KP1006.

Entry informationi

Entry nameiO16G_GEOTM
AccessioniPrimary (citable) accession number: P29094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: December 11, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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