P29094 (O16G_BACTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligo-1,6-glucosidase
EC=3.2.1.10

Alternative name(s):
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase
Sucrase-isomaltase
Short name=Isomaltase

Gene names

Name:

malL

Organism

Bacillus thermoglucosidasius (Geobacillus thermoglucosidasius)

Taxonomic identifier

1426 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Biophysicochemical properties	Temperature dependence: Extremely thermostable.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cation binding Inferred from electronic annotation. Source: InterPro oligo-1,6-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Oligo-1,6-glucosidase		PRO_0000054318
Sites
	Active site	199	1	Nucleophile By similarity
	Active site	256	1	Proton donor By similarity
	Site	330	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29094 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 925EB5924726D42A	FASTA	562	66,505
	10 20 30 40 50 60 MERVWWKEAV VYQIYPRSFY DSNGDGIGDI RGIIAKLDYL KELGVDVVWL SPVYKSPNDD 70 80 90 100 110 120 NGYDISDYRD IMDEFGTMAD WKTMLEEMHK RGIKLVMDLV VNHTSDEHPW FIESRKSKDN 130 140 150 160 170 180 PYRDYYIWRP GKNGKEPNNW ESVFSGSAWE YDEMTGEYYL HLFSKKQPDL NWENPKVRRE 190 200 210 220 230 240 VYEMMKFWLD KGVDGFRMDV INMISKVPEL PDGEPQSGKK YASGSRYYMN GPRVHEFLQE 250 260 270 280 290 300 MNREVLSKYD IMTVGETPGV TPKEGILYTD PSRRELNMVF QFEHMDLDSG PGGKWDIRPW 310 320 330 340 350 360 SLADLKKTMT KWQKELEGKG WNSLYLNNHD QPRAVSRFGD DGKYRVESAK MLATFLHMMQ 370 380 390 400 410 420 GTPYIYQGEE IGMTNVRFPS IEDYRDIETL NMYKERVEEY GEDPQEVMEK IYYKGRDNAR 430 440 450 460 470 480 TPMQWDDSEN AGFTAGTPWI PVNPNYKEIN VKAALEDPNS VFHYYKKLIQ LRKQHDIIVY 490 500 510 520 530 540 GTYDLILEDD PYIYRYTRTL GNEQLIVITN FSEKTPVFRL PDHIIYKTKE LLISNYDVDE 550 560 AEELKEIRLR PWEARVYKIR LP

References

[1]

"Proline residues responsible for thermostability occur with high frequency in the loop regions of an extremely thermostable oligo-1,6-glucosidase from Bacillus thermoglucosidasius KP1006." Watanabe K., Chishiro K., Kitamura K., Suzuki Y. J. Biol. Chem. 266:24287-24294(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12, TEMPERATURE DEPENDENCE. Strain: KP1006.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10487 Genomic DNA. Translation: BAA01368.1.
PIR	A41707.
3D structure databases
ProteinModelPortal	P29094.
SMR	P29094. Positions 1-560.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

O16G_BACTR

Accession

Primary (citable) accession number: P29094

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program