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P29094

- O16G_GEOTM

UniProt

P29094 - O16G_GEOTM

Protein

Oligo-1,6-glucosidase

Gene

malL

Organism
Geobacillus thermoglucosidasius (Bacillus thermoglucosidasius)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

    Temperature dependencei

    Extremely thermostable.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei199 – 1991NucleophileBy similarity
    Active sitei256 – 2561Proton donorBy similarity
    Sitei330 – 3301Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. oligo-1,6-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oligo-1,6-glucosidase (EC:3.2.1.10)
    Alternative name(s):
    Dextrin 6-alpha-D-glucanohydrolase
    Oligosaccharide alpha-1,6-glucosidase
    Sucrase-isomaltase
    Short name:
    Isomaltase
    Gene namesi
    Name:malL
    OrganismiGeobacillus thermoglucosidasius (Bacillus thermoglucosidasius)
    Taxonomic identifieri1426 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 562562Oligo-1,6-glucosidasePRO_0000054318Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP29094.
    SMRiP29094. Positions 1-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P29094-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERVWWKEAV VYQIYPRSFY DSNGDGIGDI RGIIAKLDYL KELGVDVVWL    50
    SPVYKSPNDD NGYDISDYRD IMDEFGTMAD WKTMLEEMHK RGIKLVMDLV 100
    VNHTSDEHPW FIESRKSKDN PYRDYYIWRP GKNGKEPNNW ESVFSGSAWE 150
    YDEMTGEYYL HLFSKKQPDL NWENPKVRRE VYEMMKFWLD KGVDGFRMDV 200
    INMISKVPEL PDGEPQSGKK YASGSRYYMN GPRVHEFLQE MNREVLSKYD 250
    IMTVGETPGV TPKEGILYTD PSRRELNMVF QFEHMDLDSG PGGKWDIRPW 300
    SLADLKKTMT KWQKELEGKG WNSLYLNNHD QPRAVSRFGD DGKYRVESAK 350
    MLATFLHMMQ GTPYIYQGEE IGMTNVRFPS IEDYRDIETL NMYKERVEEY 400
    GEDPQEVMEK IYYKGRDNAR TPMQWDDSEN AGFTAGTPWI PVNPNYKEIN 450
    VKAALEDPNS VFHYYKKLIQ LRKQHDIIVY GTYDLILEDD PYIYRYTRTL 500
    GNEQLIVITN FSEKTPVFRL PDHIIYKTKE LLISNYDVDE AEELKEIRLR 550
    PWEARVYKIR LP 562
    Length:562
    Mass (Da):66,505
    Last modified:December 1, 1992 - v1
    Checksum:i925EB5924726D42A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10487 Genomic DNA. Translation: BAA01368.1.
    PIRiA41707.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10487 Genomic DNA. Translation: BAA01368.1 .
    PIRi A41707.

    3D structure databases

    ProteinModelPortali P29094.
    SMRi P29094. Positions 1-560.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Proline residues responsible for thermostability occur with high frequency in the loop regions of an extremely thermostable oligo-1,6-glucosidase from Bacillus thermoglucosidasius KP1006."
      Watanabe K., Chishiro K., Kitamura K., Suzuki Y.
      J. Biol. Chem. 266:24287-24294(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12, TEMPERATURE DEPENDENCE.
      Strain: KP1006.

    Entry informationi

    Entry nameiO16G_GEOTM
    AccessioniPrimary (citable) accession number: P29094
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3