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P29094

- O16G_GEOTM

UniProt

P29094 - O16G_GEOTM

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Protein

Oligo-1,6-glucosidase

Gene

malL

Organism
Geobacillus thermoglucosidasius (Bacillus thermoglucosidasius)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Temperature dependencei

Extremely thermostable.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991NucleophileBy similarity
Active sitei256 – 2561Proton donorBy similarity
Sitei330 – 3301Transition state stabilizerBy similarity

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. oligo-1,6-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase (EC:3.2.1.10)
Alternative name(s):
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase
Sucrase-isomaltase
Short name:
Isomaltase
Gene namesi
Name:malL
OrganismiGeobacillus thermoglucosidasius (Bacillus thermoglucosidasius)
Taxonomic identifieri1426 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Oligo-1,6-glucosidasePRO_0000054318Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP29094.
SMRiP29094. Positions 1-560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P29094-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERVWWKEAV VYQIYPRSFY DSNGDGIGDI RGIIAKLDYL KELGVDVVWL
60 70 80 90 100
SPVYKSPNDD NGYDISDYRD IMDEFGTMAD WKTMLEEMHK RGIKLVMDLV
110 120 130 140 150
VNHTSDEHPW FIESRKSKDN PYRDYYIWRP GKNGKEPNNW ESVFSGSAWE
160 170 180 190 200
YDEMTGEYYL HLFSKKQPDL NWENPKVRRE VYEMMKFWLD KGVDGFRMDV
210 220 230 240 250
INMISKVPEL PDGEPQSGKK YASGSRYYMN GPRVHEFLQE MNREVLSKYD
260 270 280 290 300
IMTVGETPGV TPKEGILYTD PSRRELNMVF QFEHMDLDSG PGGKWDIRPW
310 320 330 340 350
SLADLKKTMT KWQKELEGKG WNSLYLNNHD QPRAVSRFGD DGKYRVESAK
360 370 380 390 400
MLATFLHMMQ GTPYIYQGEE IGMTNVRFPS IEDYRDIETL NMYKERVEEY
410 420 430 440 450
GEDPQEVMEK IYYKGRDNAR TPMQWDDSEN AGFTAGTPWI PVNPNYKEIN
460 470 480 490 500
VKAALEDPNS VFHYYKKLIQ LRKQHDIIVY GTYDLILEDD PYIYRYTRTL
510 520 530 540 550
GNEQLIVITN FSEKTPVFRL PDHIIYKTKE LLISNYDVDE AEELKEIRLR
560
PWEARVYKIR LP
Length:562
Mass (Da):66,505
Last modified:December 1, 1992 - v1
Checksum:i925EB5924726D42A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10487 Genomic DNA. Translation: BAA01368.1.
PIRiA41707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10487 Genomic DNA. Translation: BAA01368.1 .
PIRi A41707.

3D structure databases

ProteinModelPortali P29094.
SMRi P29094. Positions 1-560.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Proline residues responsible for thermostability occur with high frequency in the loop regions of an extremely thermostable oligo-1,6-glucosidase from Bacillus thermoglucosidasius KP1006."
    Watanabe K., Chishiro K., Kitamura K., Suzuki Y.
    J. Biol. Chem. 266:24287-24294(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12, TEMPERATURE DEPENDENCE.
    Strain: KP1006.

Entry informationi

Entry nameiO16G_GEOTM
AccessioniPrimary (citable) accession number: P29094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3