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P29093 (O16G_BACF5) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oligo-1,6-glucosidase

EC=3.2.1.10
Alternative name(s):
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase
Sucrase-isomaltase
Short name=Isomaltase
Gene names
Name:malL
OrganismBacillus sp. (strain F5)
Taxonomic identifier268806 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncation binding

Inferred from electronic annotation. Source: InterPro

oligo-1,6-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 509508Oligo-1,6-glucosidase
PRO_0000054316

Sites

Active site1981Nucleophile By similarity
Active site2541Proton donor By similarity
Site3311Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
P29093 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 0D1880055B35F692

FASTA50959,909
        10         20         30         40         50         60 
MSQWWKEAVV YQIYPRSFYD SNGDGFGDLQ GVIQKLDYIK RLGADVIWLC PVFDSPQDDN 

        70         80         90        100        110        120 
GYDISDYRSI YEKFGTNDDM FQLIDEVHKR GMKIIMDLVV NHSSDEHAWF AESRKSKDNP 

       130        140        150        160        170        180 
YRDYYFWKDP KADGSEPNNW GAIFSGPAWS AMSTAQYYLH YFSKKQPDLN WENEAVRREV 

       190        200        210        220        230        240 
YDLMTFWMDR GVDGWRMDVI GSISKFVDFP DYETDDSRPY VVGRYHSNGP RLHEFIQEMN 

       250        260        270        280        290        300 
REVLSRYDCM TVGEAGGSDV EEAKKYTDPS RHELNMIFTF EHMDIDTKQH SPNGKWQMKP 

       310        320        330        340        350        360 
FDPIALKKTM TRWQTALMNV GWNTLYFENH DQPRVISAGA MTRELRKQSR QSISNSSARH 

       370        380        390        400        410        420 
EGNPFIYQGE EIGMTNSEMP LEMYDDLEIK NAYRELVIEN KTMTEEDFRK AVAKKGRDHA 

       430        440        450        460        470        480 
RTPMQWDDGK YAGFTDGEAW LAVNPRYQEI NVKESLADED SIFYYYQKLI GLRKQNKVIV 

       490        500 
YGDYRLLLEE DPRIFAYIRE YRGEKLLVP 

« Hide

References

[1]"Nucleotide sequence of alkalophilic Bacillus oligo-1,6-glucosidase gene and the properties of the gene product in Escherichia coli HB101."
Yamamoto M., Horikoshi K.
Denpun Kagaku 37:137-144(1990)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00638 Genomic DNA. Translation: BAA00534.1.
PIRJQ0535.

3D structure databases

ProteinModelPortalP29093.
SMRP29093. Positions 2-508.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameO16G_BACF5
AccessionPrimary (citable) accession number: P29093
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries