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P29092 (MYR3_SINAL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myrosinase MB3
EC=3.2.1.147
Alternative name(s):
Sinigrinase
Thioglucosidase
OrganismSinapis alba (White mustard) (Brassica hirta)
Taxonomic identifier3728 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Sinapis

Protein attributes

Sequence length544 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

Catalytic activity

A thioglucoside + H2O = a sugar + a thiol.

Cofactor

Binds 1 ascorbate molecule per subunit By similarity.

Subunit structure

Homodimer.

Subcellular location

Vacuole.

Tissue specificity

In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Cellular componentVacuole
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentvacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

thioglucosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 544524Myrosinase MB3
PRO_0000011775

Sites

Active site4261Nucleophile By similarity
Metal binding761Zinc; shared with dimeric partner By similarity
Metal binding901Zinc; shared with dimeric partner By similarity
Binding site591Substrate By similarity
Binding site1611Substrate By similarity
Binding site2061Substrate By similarity
Binding site2071Ascorbate cofactor By similarity
Binding site2811Ascorbate cofactor By similarity
Binding site4261Substrate By similarity
Binding site4811Substrate By similarity

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation2401N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation3821N-linked (GlcNAc...) Potential
Glycosylation5171N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 455 By similarity
Disulfide bond34 ↔ 451 By similarity
Disulfide bond226 ↔ 236 By similarity

Sequences

Sequence LengthMass (Da)Tools
P29092 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 3971D3304CF75C28

FASTA54462,050
        10         20         30         40         50         60 
MKLLHGLALV FLLAAASCKA DEEITCEENE PFTCSNTDIL SSKNFGKDFI FGVASSAYQI 

        70         80         90        100        110        120 
EGGRGRGVNV WDGFSHRYPE KSGSDLKNGD TSCESYTRWK KDVEIMGELN ATGYRFSFAW 

       130        140        150        160        170        180 
SRIVPKGKVS RGVDQAGLDY YHNLIDALLE KNITPFVTLF HWDLPQTLQD EYEGFLDRQI 

       190        200        210        220        230        240 
IQDFKDYADL CFKEFGGKVK NWITINQLYT VPTRGYALGT DAPGRCSPKV DTKQRCYGGN 

       250        260        270        280        290        300 
SSTEPYIVAH NQLLAHAAIV DLYRTNYAFQ NGKIGPVMIT RWFLPYDESD PACIEAAERM 

       310        320        330        340        350        360 
NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEAEAELV AGSYDFLGLN YYVTQYAKPK 

       370        380        390        400        410        420 
PNPYPSETHT ALMDAGVDLT FNNSRGEYPG PVFAEDANSY YYPKGIYYVM DYFKTKYNNP 

       430        440        450        460        470        480 
LIYITENGIS TPGSESRCEA IADYKRINYL CSHLCFLRKV IREKGVNIRG YFAWALGDNY 

       490        500        510        520        530        540 
EFCKGFTVRF GLSYVNWDDL DDRNLKESGK WYQRFINGTA KNPVKQDFLR SSLSSQSQKK 


RLAC

« Hide

References

[1]"The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family."
Xue J., Lenman M., Falk A., Rask L.
Plant Mol. Biol. 18:387-398(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Maxi.
Tissue: Seed.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X59879 mRNA. Translation: CAA42534.1.
PIRS19149.

3D structure databases

ProteinModelPortalP29092.
SMRP29092. Positions 23-517.
ModBaseSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMYR3_SINAL
AccessionPrimary (citable) accession number: P29092
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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