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P29092

- MYR3_SINAL

UniProt

P29092 - MYR3_SINAL

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Protein
Myrosinase MB3
Gene
N/A
Organism
Sinapis alba (White mustard) (Brassica hirta)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.

Cofactori

Binds 1 ascorbate molecule per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591Substrate By similarity
Metal bindingi76 – 761Zinc; shared with dimeric partner By similarity
Metal bindingi90 – 901Zinc; shared with dimeric partner By similarity
Binding sitei161 – 1611Substrate By similarity
Binding sitei206 – 2061Substrate By similarity
Binding sitei207 – 2071Ascorbate cofactor By similarity
Binding sitei281 – 2811Ascorbate cofactor By similarity
Active sitei426 – 4261Nucleophile By similarity
Binding sitei426 – 4261Substrate By similarity
Binding sitei481 – 4811Substrate By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. thioglucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase MB3 (EC:3.2.1.147)
Alternative name(s):
Sinigrinase
Thioglucosidase
OrganismiSinapis alba (White mustard) (Brassica hirta)
Taxonomic identifieri3728 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeSinapis

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020
Add
BLAST
Chaini21 – 544524Myrosinase MB3
PRO_0000011775Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 455 By similarity
Disulfide bondi34 ↔ 451 By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi226 ↔ 236 By similarity
Glycosylationi240 – 2401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi331 – 3311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi382 – 3821N-linked (GlcNAc...) Reviewed prediction
Glycosylationi517 – 5171N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP29092.
SMRiP29092. Positions 23-517.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29092-1 [UniParc]FASTAAdd to Basket

« Hide

MKLLHGLALV FLLAAASCKA DEEITCEENE PFTCSNTDIL SSKNFGKDFI    50
FGVASSAYQI EGGRGRGVNV WDGFSHRYPE KSGSDLKNGD TSCESYTRWK 100
KDVEIMGELN ATGYRFSFAW SRIVPKGKVS RGVDQAGLDY YHNLIDALLE 150
KNITPFVTLF HWDLPQTLQD EYEGFLDRQI IQDFKDYADL CFKEFGGKVK 200
NWITINQLYT VPTRGYALGT DAPGRCSPKV DTKQRCYGGN SSTEPYIVAH 250
NQLLAHAAIV DLYRTNYAFQ NGKIGPVMIT RWFLPYDESD PACIEAAERM 300
NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEAEAELV AGSYDFLGLN 350
YYVTQYAKPK PNPYPSETHT ALMDAGVDLT FNNSRGEYPG PVFAEDANSY 400
YYPKGIYYVM DYFKTKYNNP LIYITENGIS TPGSESRCEA IADYKRINYL 450
CSHLCFLRKV IREKGVNIRG YFAWALGDNY EFCKGFTVRF GLSYVNWDDL 500
DDRNLKESGK WYQRFINGTA KNPVKQDFLR SSLSSQSQKK RLAC 544
Length:544
Mass (Da):62,050
Last modified:December 1, 1992 - v1
Checksum:i3971D3304CF75C28
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59879 mRNA. Translation: CAA42534.1.
PIRiS19149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59879 mRNA. Translation: CAA42534.1 .
PIRi S19149.

3D structure databases

ProteinModelPortali P29092.
SMRi P29092. Positions 23-517.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family."
    Xue J., Lenman M., Falk A., Rask L.
    Plant Mol. Biol. 18:387-398(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Maxi.
    Tissue: Seed.

Entry informationi

Entry nameiMYR3_SINAL
AccessioniPrimary (citable) accession number: P29092
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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