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P29092

- MYR3_SINAL

UniProt

P29092 - MYR3_SINAL

Protein

Myrosinase MB3

Gene
N/A
Organism
Sinapis alba (White mustard) (Brassica hirta)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

    Catalytic activityi

    A thioglucoside + H2O = a sugar + a thiol.

    Cofactori

    Binds 1 ascorbate molecule per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei59 – 591SubstrateBy similarity
    Metal bindingi76 – 761Zinc; shared with dimeric partnerBy similarity
    Metal bindingi90 – 901Zinc; shared with dimeric partnerBy similarity
    Binding sitei161 – 1611SubstrateBy similarity
    Binding sitei206 – 2061SubstrateBy similarity
    Binding sitei207 – 2071AscorbateBy similarity
    Binding sitei281 – 2811AscorbateBy similarity
    Active sitei426 – 4261NucleophilePROSITE-ProRule annotation
    Binding sitei426 – 4261SubstrateBy similarity
    Binding sitei481 – 4811SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. thioglucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myrosinase MB3 (EC:3.2.1.147)
    Alternative name(s):
    Sinigrinase
    Thioglucosidase
    OrganismiSinapis alba (White mustard) (Brassica hirta)
    Taxonomic identifieri3728 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeSinapis

    Subcellular locationi

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Add
    BLAST
    Chaini21 – 544524Myrosinase MB3PRO_0000011775Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 455By similarity
    Disulfide bondi34 ↔ 451By similarity
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi226 ↔ 236By similarity
    Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi382 – 3821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi517 – 5171N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP29092.
    SMRiP29092. Positions 23-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29092-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLLHGLALV FLLAAASCKA DEEITCEENE PFTCSNTDIL SSKNFGKDFI    50
    FGVASSAYQI EGGRGRGVNV WDGFSHRYPE KSGSDLKNGD TSCESYTRWK 100
    KDVEIMGELN ATGYRFSFAW SRIVPKGKVS RGVDQAGLDY YHNLIDALLE 150
    KNITPFVTLF HWDLPQTLQD EYEGFLDRQI IQDFKDYADL CFKEFGGKVK 200
    NWITINQLYT VPTRGYALGT DAPGRCSPKV DTKQRCYGGN SSTEPYIVAH 250
    NQLLAHAAIV DLYRTNYAFQ NGKIGPVMIT RWFLPYDESD PACIEAAERM 300
    NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEAEAELV AGSYDFLGLN 350
    YYVTQYAKPK PNPYPSETHT ALMDAGVDLT FNNSRGEYPG PVFAEDANSY 400
    YYPKGIYYVM DYFKTKYNNP LIYITENGIS TPGSESRCEA IADYKRINYL 450
    CSHLCFLRKV IREKGVNIRG YFAWALGDNY EFCKGFTVRF GLSYVNWDDL 500
    DDRNLKESGK WYQRFINGTA KNPVKQDFLR SSLSSQSQKK RLAC 544
    Length:544
    Mass (Da):62,050
    Last modified:December 1, 1992 - v1
    Checksum:i3971D3304CF75C28
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59879 mRNA. Translation: CAA42534.1.
    PIRiS19149.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59879 mRNA. Translation: CAA42534.1 .
    PIRi S19149.

    3D structure databases

    ProteinModelPortali P29092.
    SMRi P29092. Positions 23-517.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family."
      Xue J., Lenman M., Falk A., Rask L.
      Plant Mol. Biol. 18:387-398(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: cv. Maxi.
      Tissue: Seed.

    Entry informationi

    Entry nameiMYR3_SINAL
    AccessioniPrimary (citable) accession number: P29092
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3