P29084 (T2EB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor IIE subunit beta
Short name=TFIIE-beta

Alternative name(s):
General transcription factor IIE subunit 2

Gene names

Name:	GTF2E2
Synonyms:	TF2E2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Subunit structure	Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H. Interacts with ATF7IP. Ref.6 Ref.7
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TFIIE beta subunit family. Contains 1 TFIIE beta DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription elongation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nucleoplasm Traceable author statement. Source: Reactome transcription factor TFIIE complex Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MCC	P23508	2	EBI-2853321,EBI-307531

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 291

291

Transcription initiation factor IIE subunit beta

PRO_0000211226

Regions

DNA binding

66 – 146

81

TFIIE beta

Compositional bias

26 – 36

11

Poly-Ser

Compositional bias

37 – 42

6

Lys-rich (basic)

Compositional bias

251 – 271

21

Arg/Lys-rich (basic)

Natural variations

Natural variant

133

1

I → T.
Corresponds to variant rs2229299 [ dbSNP | Ensembl ].

VAR_052281

Natural variant

183

1

K → R.
Corresponds to variant rs2978277 [ dbSNP | Ensembl ].

VAR_039003

Experimental info

Sequence conflict

53

1

Q → E in AAG39077. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

291

Helix Strand Turn

Details...

Helix

67 – 70

4

Helix

75 – 91

17

Helix

99 – 106

8

Beta strand

109 – 111

3

Helix

113 – 120

8

Helix

123 – 126

4

Beta strand

130 – 132

3

Beta strand

134 – 139

6

Turn

142 – 144

3

Sequences

	Sequence		Length	Mass (Da)
	P29084 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 20F6BDFF2E06E5E7	FASTA	291	33,044
	10 20 30 40 50 60 MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG SKQNSDHSNG 70 80 90 100 110 120 SFNLKALSGS SGYKFGVLAK IVNYMKTRHQ RGDTHPLTLD EILDETQHLD IGLKQKQWLM 130 140 150 160 170 180 TEALVNNPKI EVIDGKYAFK PKYNVRDKKA LLRLLDQHDQ RGLGGILLED IEEALPNSQK 190 200 210 220 230 240 AVKALGDQIL FVNRPDKKKI LFFNDKSCQF SVDEEFQKLW RSVTVDSMDE EKIEEYLKRQ 250 260 270 280 290 GISSMQESGP KKVAPIQRRK KPASQKKRRF KTHNEHLAGV LKDYSDITSS K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and functional properties of human general transcription factor IIE." Peterson M.G., Inostroza J., Maxon M.E., Flores O., Admon A., Reinberg D., Tjian R. Nature 354:369-373(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Conserved sequence motifs in the small subunit of human general transcription factor TFIIE." Sumimoto H., Ohkuma Y., Sinn E., Kato H., Shimasaki S., Horikoshi M., Roeder R.G. Nature 354:401-404(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[3]	Schertzer M., Wood S. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[6]	"Functional interaction of general transcription initiation factor TFIIE with general chromatin factor SPT16/CDC68." Kang S.-W., Kuzuhara T., Horikoshi M. Genes Cells 5:251-263(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SUPT16H.
[7]	"MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity." Liu L., Ishihara K., Ichimura T., Fujita N., Hino S., Tomita S., Watanabe S., Saitoh N., Ito T., Nakao M. J. Biol. Chem. 284:5165-5174(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ATF7IP.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S67861 mRNA. Translation: AAB20414.1.
X63469 mRNA. Translation: CAA45069.1.
AF292062 , AF292056, AF292057, AF292058, AF292059, AF292060, AF292061 Genomic DNA. Translation: AAG39077.1.
CH471080 Genomic DNA. Translation: EAW63446.1.
CH471080 Genomic DNA. Translation: EAW63449.1.
BC030572 mRNA. Translation: AAH30572.1.

IPI

IPI00019981.

PIR

S29292.

RefSeq

NP_002086.1. NM_002095.4.

UniGene

Hs.77100.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D8J	NMR	-	A	66-146	[»]
1D8K	NMR	-	A	66-146	[»]

ProteinModelPortal

P29084.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-716N.

IntAct

P29084. 2 interactions.

MINT

MINT-1894071.

STRING

9606.ENSP00000348168.

PTM databases

PhosphoSite

P29084.

Polymorphism databases

DMDM

135232.

Proteomic databases

PaxDb

P29084.

PeptideAtlas

P29084.

PRIDE

P29084.

Protocols and materials databases

DNASU

2961.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000355904; ENSP00000348168; ENSG00000197265.

GeneID

2961.

KEGG

hsa:2961.

UCSC

uc003xig.3. human.

Organism-specific databases

CTD

2961.

GeneCards

GC08M030429.

HGNC

HGNC:4651. GTF2E2.

HPA

CAB009603.
HPA025065.

MIM

189964. gene.

neXtProt

NX_P29084.

PharmGKB

PA29037.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5174.

HOGENOM

HOG000008149.

HOVERGEN

HBG009400.

InParanoid

P29084.

KO

K03137.

OMA

LGDQIIF.

OrthoDB

EOG470TJ9.

PhylomeDB

P29084.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.
REACT_1788. Transcription.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P29084.

Bgee

P29084.

CleanEx

HS_GTF2E2.

Genevestigator

P29084.

GermOnline

ENSG00000197265. Homo sapiens.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR016656. TFIIE-bsu.
IPR003166. TFIIE_bsu_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF02186. TFIIE_beta. 1 hit.
[Graphical view]

PIRSF

PIRSF016398. TFIIE-beta. 1 hit.

PROSITE

PS51351. TFIIE_BETA_C. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P29084.

GenomeRNAi

2961.

NextBio

11738.

SOURCE

Search...

Entry information

Entry name

T2EB_HUMAN

Accession

Primary (citable) accession number: P29084
Secondary accession number(s): D3DSV2, Q9H2B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 129 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.