SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P29084

- T2EB_HUMAN

UniProt

P29084 - T2EB_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Transcription initiation factor IIE subunit beta
Gene
GTF2E2, TF2E2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi66 – 14681TFIIE beta
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. poly(A) RNA binding Source: UniProtKB
  3. protein binding Source: UniProtKB

GO - Biological processi

  1. gene expression Source: Reactome
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription elongation from RNA polymerase II promoter Source: Reactome
  4. transcription from RNA polymerase II promoter Source: Reactome
  5. transcription initiation from RNA polymerase II promoter Source: Reactome
  6. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_6233. Transcription of the HIV genome.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6332. HIV Transcription Initiation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIE subunit beta
Short name:
TFIIE-beta
Alternative name(s):
General transcription factor IIE subunit 2
Gene namesi
Name:GTF2E2
Synonyms:TF2E2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:4651. GTF2E2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. nucleoplasm Source: Reactome
  3. nucleus Source: HPA
  4. transcription factor TFIIE complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29037.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Transcription initiation factor IIE subunit beta
PRO_0000211226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei74 – 741N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP29084.
PaxDbiP29084.
PeptideAtlasiP29084.
PRIDEiP29084.

PTM databases

PhosphoSiteiP29084.

Expressioni

Gene expression databases

ArrayExpressiP29084.
BgeeiP29084.
CleanExiHS_GTF2E2.
GenevestigatoriP29084.

Organism-specific databases

HPAiCAB009603.
HPA004816.
HPA025065.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H. Interacts with ATF7IP.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GTF2E1P290832EBI-2853321,EBI-5462215
MCCP235082EBI-2853321,EBI-307531

Protein-protein interaction databases

BioGridi109216. 30 interactions.
DIPiDIP-716N.
IntActiP29084. 3 interactions.
MINTiMINT-1894071.
STRINGi9606.ENSP00000348168.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi67 – 704
Helixi75 – 9117
Helixi99 – 1068
Beta strandi109 – 1113
Helixi113 – 1208
Helixi123 – 1264
Beta strandi130 – 1323
Beta strandi134 – 1396
Turni142 – 1443

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D8JNMR-A66-146[»]
1D8KNMR-A66-146[»]
ProteinModelPortaliP29084.
SMRiP29084. Positions 66-146.

Miscellaneous databases

EvolutionaryTraceiP29084.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 3611Poly-Ser
Add
BLAST
Compositional biasi37 – 426Lys-rich (basic)
Compositional biasi251 – 27121Arg/Lys-rich (basic)
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5174.
HOGENOMiHOG000008149.
HOVERGENiHBG009400.
InParanoidiP29084.
KOiK03137.
OMAiLGDQIIF.
OrthoDBiEOG71ZP2B.
PhylomeDBiP29084.
TreeFamiTF105901.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR016656. TFIIE-bsu.
IPR003166. TFIIE_bsu_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF02186. TFIIE_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF016398. TFIIE-beta. 1 hit.
PROSITEiPS51351. TFIIE_BETA_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29084-1 [UniParc]FASTAAdd to Basket

« Hide

MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG    50
SKQNSDHSNG SFNLKALSGS SGYKFGVLAK IVNYMKTRHQ RGDTHPLTLD 100
EILDETQHLD IGLKQKQWLM TEALVNNPKI EVIDGKYAFK PKYNVRDKKA 150
LLRLLDQHDQ RGLGGILLED IEEALPNSQK AVKALGDQIL FVNRPDKKKI 200
LFFNDKSCQF SVDEEFQKLW RSVTVDSMDE EKIEEYLKRQ GISSMQESGP 250
KKVAPIQRRK KPASQKKRRF KTHNEHLAGV LKDYSDITSS K 291
Length:291
Mass (Da):33,044
Last modified:December 1, 1992 - v1
Checksum:i20F6BDFF2E06E5E7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti133 – 1331I → T.
Corresponds to variant rs2229299 [ dbSNP | Ensembl ].
VAR_052281
Natural varianti183 – 1831K → R.
Corresponds to variant rs2978277 [ dbSNP | Ensembl ].
VAR_039003

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531Q → E in AAG39077. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S67861 mRNA. Translation: AAB20414.1.
X63469 mRNA. Translation: CAA45069.1.
AF292062
, AF292056, AF292057, AF292058, AF292059, AF292060, AF292061 Genomic DNA. Translation: AAG39077.1.
CH471080 Genomic DNA. Translation: EAW63446.1.
CH471080 Genomic DNA. Translation: EAW63449.1.
BC030572 mRNA. Translation: AAH30572.1.
CCDSiCCDS6078.1.
PIRiS29292.
RefSeqiNP_002086.1. NM_002095.4.
UniGeneiHs.77100.

Genome annotation databases

EnsembliENST00000355904; ENSP00000348168; ENSG00000197265.
GeneIDi2961.
KEGGihsa:2961.
UCSCiuc003xig.3. human.

Polymorphism databases

DMDMi135232.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S67861 mRNA. Translation: AAB20414.1 .
X63469 mRNA. Translation: CAA45069.1 .
AF292062
, AF292056 , AF292057 , AF292058 , AF292059 , AF292060 , AF292061 Genomic DNA. Translation: AAG39077.1 .
CH471080 Genomic DNA. Translation: EAW63446.1 .
CH471080 Genomic DNA. Translation: EAW63449.1 .
BC030572 mRNA. Translation: AAH30572.1 .
CCDSi CCDS6078.1.
PIRi S29292.
RefSeqi NP_002086.1. NM_002095.4.
UniGenei Hs.77100.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1D8J NMR - A 66-146 [» ]
1D8K NMR - A 66-146 [» ]
ProteinModelPortali P29084.
SMRi P29084. Positions 66-146.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109216. 30 interactions.
DIPi DIP-716N.
IntActi P29084. 3 interactions.
MINTi MINT-1894071.
STRINGi 9606.ENSP00000348168.

PTM databases

PhosphoSitei P29084.

Polymorphism databases

DMDMi 135232.

Proteomic databases

MaxQBi P29084.
PaxDbi P29084.
PeptideAtlasi P29084.
PRIDEi P29084.

Protocols and materials databases

DNASUi 2961.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000355904 ; ENSP00000348168 ; ENSG00000197265 .
GeneIDi 2961.
KEGGi hsa:2961.
UCSCi uc003xig.3. human.

Organism-specific databases

CTDi 2961.
GeneCardsi GC08M030429.
HGNCi HGNC:4651. GTF2E2.
HPAi CAB009603.
HPA004816.
HPA025065.
MIMi 189964. gene.
neXtProti NX_P29084.
PharmGKBi PA29037.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5174.
HOGENOMi HOG000008149.
HOVERGENi HBG009400.
InParanoidi P29084.
KOi K03137.
OMAi LGDQIIF.
OrthoDBi EOG71ZP2B.
PhylomeDBi P29084.
TreeFami TF105901.

Enzyme and pathway databases

Reactomei REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_6233. Transcription of the HIV genome.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6332. HIV Transcription Initiation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

EvolutionaryTracei P29084.
GeneWikii GTF2E2.
GenomeRNAii 2961.
NextBioi 11738.
PROi P29084.
SOURCEi Search...

Gene expression databases

ArrayExpressi P29084.
Bgeei P29084.
CleanExi HS_GTF2E2.
Genevestigatori P29084.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR016656. TFIIE-bsu.
IPR003166. TFIIE_bsu_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF02186. TFIIE_beta. 1 hit.
[Graphical view ]
PIRSFi PIRSF016398. TFIIE-beta. 1 hit.
PROSITEi PS51351. TFIIE_BETA_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and functional properties of human general transcription factor IIE."
    Peterson M.G., Inostroza J., Maxon M.E., Flores O., Admon A., Reinberg D., Tjian R.
    Nature 354:369-373(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Conserved sequence motifs in the small subunit of human general transcription factor TFIIE."
    Sumimoto H., Ohkuma Y., Sinn E., Kato H., Shimasaki S., Horikoshi M., Roeder R.G.
    Nature 354:401-404(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  3. Schertzer M., Wood S.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  6. "Functional interaction of general transcription initiation factor TFIIE with general chromatin factor SPT16/CDC68."
    Kang S.-W., Kuzuhara T., Horikoshi M.
    Genes Cells 5:251-263(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SUPT16H.
  7. "MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity."
    Liu L., Ishihara K., Ichimura T., Fujita N., Hino S., Tomita S., Watanabe S., Saitoh N., Ito T., Nakao M.
    J. Biol. Chem. 284:5165-5174(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATF7IP.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiT2EB_HUMAN
AccessioniPrimary (citable) accession number: P29084
Secondary accession number(s): D3DSV2, Q9H2B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 3, 2014
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi