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P29084

- T2EB_HUMAN

UniProt

P29084 - T2EB_HUMAN

Protein

Transcription initiation factor IIE subunit beta

Gene

GTF2E2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi66 – 14681TFIIE betaPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. poly(A) RNA binding Source: UniProtKB
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. gene expression Source: Reactome
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription elongation from RNA polymerase II promoter Source: Reactome
    4. transcription from RNA polymerase II promoter Source: Reactome
    5. transcription initiation from RNA polymerase II promoter Source: Reactome
    6. viral process Source: Reactome

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_6233. Transcription of the HIV genome.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6332. HIV Transcription Initiation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor IIE subunit beta
    Short name:
    TFIIE-beta
    Alternative name(s):
    General transcription factor IIE subunit 2
    Gene namesi
    Name:GTF2E2
    Synonyms:TF2E2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:4651. GTF2E2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. nucleoplasm Source: Reactome
    3. nucleus Source: HPA
    4. transcription factor TFIIE complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29037.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Transcription initiation factor IIE subunit betaPRO_0000211226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei74 – 741N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP29084.
    PaxDbiP29084.
    PeptideAtlasiP29084.
    PRIDEiP29084.

    PTM databases

    PhosphoSiteiP29084.

    Expressioni

    Gene expression databases

    ArrayExpressiP29084.
    BgeeiP29084.
    CleanExiHS_GTF2E2.
    GenevestigatoriP29084.

    Organism-specific databases

    HPAiCAB009603.
    HPA004816.
    HPA025065.

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H. Interacts with ATF7IP.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GTF2E1P290832EBI-2853321,EBI-5462215
    MCCP235082EBI-2853321,EBI-307531

    Protein-protein interaction databases

    BioGridi109216. 30 interactions.
    DIPiDIP-716N.
    IntActiP29084. 3 interactions.
    MINTiMINT-1894071.
    STRINGi9606.ENSP00000348168.

    Structurei

    Secondary structure

    1
    291
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi67 – 704
    Helixi75 – 9117
    Helixi99 – 1068
    Beta strandi109 – 1113
    Helixi113 – 1208
    Helixi123 – 1264
    Beta strandi130 – 1323
    Beta strandi134 – 1396
    Turni142 – 1443

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1D8JNMR-A66-146[»]
    1D8KNMR-A66-146[»]
    ProteinModelPortaliP29084.
    SMRiP29084. Positions 66-146.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP29084.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi26 – 3611Poly-SerAdd
    BLAST
    Compositional biasi37 – 426Lys-rich (basic)
    Compositional biasi251 – 27121Arg/Lys-rich (basic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the TFIIE beta subunit family.PROSITE-ProRule annotation
    Contains 1 TFIIE beta DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5174.
    HOGENOMiHOG000008149.
    HOVERGENiHBG009400.
    InParanoidiP29084.
    KOiK03137.
    OMAiLGDQIIF.
    OrthoDBiEOG71ZP2B.
    PhylomeDBiP29084.
    TreeFamiTF105901.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR016656. TFIIE-bsu.
    IPR003166. TFIIE_bsu_DNA-bd.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF02186. TFIIE_beta. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016398. TFIIE-beta. 1 hit.
    PROSITEiPS51351. TFIIE_BETA_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29084-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG    50
    SKQNSDHSNG SFNLKALSGS SGYKFGVLAK IVNYMKTRHQ RGDTHPLTLD 100
    EILDETQHLD IGLKQKQWLM TEALVNNPKI EVIDGKYAFK PKYNVRDKKA 150
    LLRLLDQHDQ RGLGGILLED IEEALPNSQK AVKALGDQIL FVNRPDKKKI 200
    LFFNDKSCQF SVDEEFQKLW RSVTVDSMDE EKIEEYLKRQ GISSMQESGP 250
    KKVAPIQRRK KPASQKKRRF KTHNEHLAGV LKDYSDITSS K 291
    Length:291
    Mass (Da):33,044
    Last modified:December 1, 1992 - v1
    Checksum:i20F6BDFF2E06E5E7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531Q → E in AAG39077. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti133 – 1331I → T.
    Corresponds to variant rs2229299 [ dbSNP | Ensembl ].
    VAR_052281
    Natural varianti183 – 1831K → R.
    Corresponds to variant rs2978277 [ dbSNP | Ensembl ].
    VAR_039003

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S67861 mRNA. Translation: AAB20414.1.
    X63469 mRNA. Translation: CAA45069.1.
    AF292062
    , AF292056, AF292057, AF292058, AF292059, AF292060, AF292061 Genomic DNA. Translation: AAG39077.1.
    CH471080 Genomic DNA. Translation: EAW63446.1.
    CH471080 Genomic DNA. Translation: EAW63449.1.
    BC030572 mRNA. Translation: AAH30572.1.
    CCDSiCCDS6078.1.
    PIRiS29292.
    RefSeqiNP_002086.1. NM_002095.4.
    UniGeneiHs.77100.

    Genome annotation databases

    EnsembliENST00000355904; ENSP00000348168; ENSG00000197265.
    GeneIDi2961.
    KEGGihsa:2961.
    UCSCiuc003xig.3. human.

    Polymorphism databases

    DMDMi135232.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S67861 mRNA. Translation: AAB20414.1 .
    X63469 mRNA. Translation: CAA45069.1 .
    AF292062
    , AF292056 , AF292057 , AF292058 , AF292059 , AF292060 , AF292061 Genomic DNA. Translation: AAG39077.1 .
    CH471080 Genomic DNA. Translation: EAW63446.1 .
    CH471080 Genomic DNA. Translation: EAW63449.1 .
    BC030572 mRNA. Translation: AAH30572.1 .
    CCDSi CCDS6078.1.
    PIRi S29292.
    RefSeqi NP_002086.1. NM_002095.4.
    UniGenei Hs.77100.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1D8J NMR - A 66-146 [» ]
    1D8K NMR - A 66-146 [» ]
    ProteinModelPortali P29084.
    SMRi P29084. Positions 66-146.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109216. 30 interactions.
    DIPi DIP-716N.
    IntActi P29084. 3 interactions.
    MINTi MINT-1894071.
    STRINGi 9606.ENSP00000348168.

    PTM databases

    PhosphoSitei P29084.

    Polymorphism databases

    DMDMi 135232.

    Proteomic databases

    MaxQBi P29084.
    PaxDbi P29084.
    PeptideAtlasi P29084.
    PRIDEi P29084.

    Protocols and materials databases

    DNASUi 2961.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000355904 ; ENSP00000348168 ; ENSG00000197265 .
    GeneIDi 2961.
    KEGGi hsa:2961.
    UCSCi uc003xig.3. human.

    Organism-specific databases

    CTDi 2961.
    GeneCardsi GC08M030429.
    HGNCi HGNC:4651. GTF2E2.
    HPAi CAB009603.
    HPA004816.
    HPA025065.
    MIMi 189964. gene.
    neXtProti NX_P29084.
    PharmGKBi PA29037.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5174.
    HOGENOMi HOG000008149.
    HOVERGENi HBG009400.
    InParanoidi P29084.
    KOi K03137.
    OMAi LGDQIIF.
    OrthoDBi EOG71ZP2B.
    PhylomeDBi P29084.
    TreeFami TF105901.

    Enzyme and pathway databases

    Reactomei REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_6233. Transcription of the HIV genome.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6332. HIV Transcription Initiation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

    Miscellaneous databases

    EvolutionaryTracei P29084.
    GeneWikii GTF2E2.
    GenomeRNAii 2961.
    NextBioi 11738.
    PROi P29084.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P29084.
    Bgeei P29084.
    CleanExi HS_GTF2E2.
    Genevestigatori P29084.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR016656. TFIIE-bsu.
    IPR003166. TFIIE_bsu_DNA-bd.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF02186. TFIIE_beta. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016398. TFIIE-beta. 1 hit.
    PROSITEi PS51351. TFIIE_BETA_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and functional properties of human general transcription factor IIE."
      Peterson M.G., Inostroza J., Maxon M.E., Flores O., Admon A., Reinberg D., Tjian R.
      Nature 354:369-373(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Conserved sequence motifs in the small subunit of human general transcription factor TFIIE."
      Sumimoto H., Ohkuma Y., Sinn E., Kato H., Shimasaki S., Horikoshi M., Roeder R.G.
      Nature 354:401-404(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    3. Schertzer M., Wood S.
      Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.
    6. "Functional interaction of general transcription initiation factor TFIIE with general chromatin factor SPT16/CDC68."
      Kang S.-W., Kuzuhara T., Horikoshi M.
      Genes Cells 5:251-263(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SUPT16H.
    7. "MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity."
      Liu L., Ishihara K., Ichimura T., Fujita N., Hino S., Tomita S., Watanabe S., Saitoh N., Ito T., Nakao M.
      J. Biol. Chem. 284:5165-5174(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATF7IP.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiT2EB_HUMAN
    AccessioniPrimary (citable) accession number: P29084
    Secondary accession number(s): D3DSV2, Q9H2B9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3