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Protein

General transcription factor IIE subunit 1

Gene

GTF2E1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri129 – 157C4-typeSequence analysisAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIE subunit 1
Alternative name(s):
General transcription factor IIE 56 kDa subunit
Transcription initiation factor IIE subunit alpha
Short name:
TFIIE-alpha
Gene namesi
Name:GTF2E1
Synonyms:TF2E1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:4650. GTF2E1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: CAFA
  • transcriptional preinitiation complex Source: CAFA
  • transcription factor TFIID complex Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2960.
OpenTargetsiENSG00000153767.
PharmGKBiPA29036.

Polymorphism and mutation databases

BioMutaiGTF2E1.
DMDMi116242812.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002112222 – 439General transcription factor IIE subunit 1Add BLAST438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei67N6-acetyllysineCombined sources1
Modified residuei268PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP29083.
MaxQBiP29083.
PaxDbiP29083.
PeptideAtlasiP29083.
PRIDEiP29083.

PTM databases

iPTMnetiP29083.
PhosphoSitePlusiP29083.

Miscellaneous databases

PMAP-CutDBiP29083.

Expressioni

Gene expression databases

BgeeiENSG00000153767.
CleanExiHS_GTF2E1.
ExpressionAtlasiP29083. baseline and differential.
GenevisibleiP29083. HS.

Organism-specific databases

HPAiCAB009884.
HPA056359.
HPA066881.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Interacts with TAF6/TAFII80. Interacts with ATF7IP. Interacts with varicella-zoster virus IE63 protein.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi109215. 51 interactors.
DIPiDIP-717N.
IntActiP29083. 7 interactors.
MINTiMINT-1894058.
STRINGi9606.ENSP00000283875.

Structurei

Secondary structure

1439
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 25Combined sources14
Helixi28 – 39Combined sources12
Beta strandi40 – 44Combined sources5
Helixi45 – 52Combined sources8
Helixi56 – 68Combined sources13
Beta strandi71 – 80Combined sources10
Beta strandi86 – 94Combined sources9
Helixi96 – 122Combined sources27
Beta strandi123 – 128Combined sources6
Turni130 – 132Combined sources3
Beta strandi135 – 137Combined sources3
Helixi138 – 142Combined sources5
Turni147 – 149Combined sources3
Beta strandi150 – 153Combined sources4
Turni155 – 157Combined sources3
Helixi176 – 193Combined sources18
Beta strandi380 – 383Combined sources4
Beta strandi388 – 390Combined sources3
Beta strandi395 – 398Combined sources4
Beta strandi401 – 404Combined sources4
Helixi405 – 410Combined sources6
Helixi412 – 417Combined sources6
Helixi420 – 433Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VD4NMR-A113-174[»]
2JTXNMR-A336-439[»]
2RNQNMR-A378-439[»]
2RNRNMR-A378-439[»]
5GPYX-ray2.10A1-217[»]
5IY6electron microscopy7.20Q1-439[»]
5IY7electron microscopy8.60Q1-439[»]
5IY8electron microscopy7.90Q1-439[»]
5IY9electron microscopy6.30Q1-439[»]
5IYAelectron microscopy5.40Q1-439[»]
5IYBelectron microscopy3.90Q1-439[»]
5IYCelectron microscopy3.90Q1-439[»]
5IYDelectron microscopy3.90Q1-439[»]
ProteinModelPortaliP29083.
SMRiP29083.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29083.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 104HTH TFE/IIEalpha-typePROSITE-ProRule annotationAdd BLAST91

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi378 – 393Asp/Glu-rich (acidic)Add BLAST16

Sequence similaritiesi

Belongs to the TFIIE alpha subunit family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri129 – 157C4-typeSequence analysisAdd BLAST29

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2593. Eukaryota.
COG1675. LUCA.
GeneTreeiENSGT00390000016696.
HOGENOMiHOG000007666.
HOVERGENiHBG008614.
InParanoidiP29083.
KOiK03136.
OMAiEFRCTYC.
OrthoDBiEOG091G0D8N.
PhylomeDBiP29083.
TreeFamiTF313429.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR021600. TFIIE_asu_C.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR013137. Znf_TFIIB.
PfamiView protein in Pfam
PF08271. TF_Zn_Ribbon. 1 hit.
PF11521. TFIIE-A_C. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
SMARTiView protein in SMART
SM00531. TFIIE. 1 hit.
PROSITEiView protein in PROSITE
PS51344. HTH_TFE_IIE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE
60 70 80 90 100
LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV
110 120 130 140 150
NVVKYKLDHM RRRIETDERD STNRASFKCP VCSSTFTDLE ANQLFDPMTG
160 170 180 190 200
TFRCTFCHTE VEEDESAMPK KDARTLLARF NEQIEPIYAL LRETEDVNLA
210 220 230 240 250
YEILEPEPTE IPALKQSKDH AATTAGAASL AGGHHREAWA TKGPSYEDLY
260 270 280 290 300
TQNVVINMDD QEDLHRASLE GKSAKERPIW LRESTVQGAY GSEDMKEGGI
310 320 330 340 350
DMDAFQEREE GHAGPDDNEE VMRALLIHEK KTSSAMAGSV GAAAPVTAAN
360 370 380 390 400
GSDSESETSE SDDDSPPRPA AVAVHKREED EEEDDEFEEV ADDPIVMVAG
410 420 430
RPFSYSEVSQ RPELVAQMTP EEKEAYIAMG QRMFEDLFE
Length:439
Mass (Da):49,452
Last modified:October 17, 2006 - v2
Checksum:i9234FFE3F150340B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti352S → D in CAA45068 (PubMed:1956403).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020321366P → S. Corresponds to variant dbSNP:rs3732401Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67859 mRNA. Translation: AAB20413.1.
X63468 mRNA. Translation: CAA45068.1.
CCDSiCCDS3002.1.
PIRiS29291.
RefSeqiNP_005504.2. NM_005513.2.
XP_011511046.1. XM_011512744.2.
XP_011511047.1. XM_011512745.2.
UniGeneiHs.445272.

Genome annotation databases

EnsembliENST00000283875; ENSP00000283875; ENSG00000153767.
GeneIDi2960.
KEGGihsa:2960.
UCSCiuc003edz.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67859 mRNA. Translation: AAB20413.1.
X63468 mRNA. Translation: CAA45068.1.
CCDSiCCDS3002.1.
PIRiS29291.
RefSeqiNP_005504.2. NM_005513.2.
XP_011511046.1. XM_011512744.2.
XP_011511047.1. XM_011512745.2.
UniGeneiHs.445272.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VD4NMR-A113-174[»]
2JTXNMR-A336-439[»]
2RNQNMR-A378-439[»]
2RNRNMR-A378-439[»]
5GPYX-ray2.10A1-217[»]
5IY6electron microscopy7.20Q1-439[»]
5IY7electron microscopy8.60Q1-439[»]
5IY8electron microscopy7.90Q1-439[»]
5IY9electron microscopy6.30Q1-439[»]
5IYAelectron microscopy5.40Q1-439[»]
5IYBelectron microscopy3.90Q1-439[»]
5IYCelectron microscopy3.90Q1-439[»]
5IYDelectron microscopy3.90Q1-439[»]
ProteinModelPortaliP29083.
SMRiP29083.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109215. 51 interactors.
DIPiDIP-717N.
IntActiP29083. 7 interactors.
MINTiMINT-1894058.
STRINGi9606.ENSP00000283875.

PTM databases

iPTMnetiP29083.
PhosphoSitePlusiP29083.

Polymorphism and mutation databases

BioMutaiGTF2E1.
DMDMi116242812.

Proteomic databases

EPDiP29083.
MaxQBiP29083.
PaxDbiP29083.
PeptideAtlasiP29083.
PRIDEiP29083.

Protocols and materials databases

DNASUi2960.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000283875; ENSP00000283875; ENSG00000153767.
GeneIDi2960.
KEGGihsa:2960.
UCSCiuc003edz.5. human.

Organism-specific databases

CTDi2960.
DisGeNETi2960.
GeneCardsiGTF2E1.
HGNCiHGNC:4650. GTF2E1.
HPAiCAB009884.
HPA056359.
HPA066881.
MIMi189962. gene.
neXtProtiNX_P29083.
OpenTargetsiENSG00000153767.
PharmGKBiPA29036.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2593. Eukaryota.
COG1675. LUCA.
GeneTreeiENSGT00390000016696.
HOGENOMiHOG000007666.
HOVERGENiHBG008614.
InParanoidiP29083.
KOiK03136.
OMAiEFRCTYC.
OrthoDBiEOG091G0D8N.
PhylomeDBiP29083.
TreeFamiTF313429.

Enzyme and pathway databases

ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

ChiTaRSiGTF2E1. human.
EvolutionaryTraceiP29083.
GeneWikiiGTF2E1.
GenomeRNAii2960.
PMAP-CutDBiP29083.
PROiPR:P29083.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000153767.
CleanExiHS_GTF2E1.
ExpressionAtlasiP29083. baseline and differential.
GenevisibleiP29083. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR021600. TFIIE_asu_C.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR013137. Znf_TFIIB.
PfamiView protein in Pfam
PF08271. TF_Zn_Ribbon. 1 hit.
PF11521. TFIIE-A_C. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
SMARTiView protein in SMART
SM00531. TFIIE. 1 hit.
PROSITEiView protein in PROSITE
PS51344. HTH_TFE_IIE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2EA_HUMAN
AccessioniPrimary (citable) accession number: P29083
Secondary accession number(s): Q16103
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 17, 2006
Last modified: June 7, 2017
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.