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P29083

- T2EA_HUMAN

UniProt

P29083 - T2EA_HUMAN

Protein

General transcription factor IIE subunit 1

Gene

GTF2E1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri129 – 15729C4-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. gene expression Source: Reactome
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription elongation from RNA polymerase II promoter Source: Reactome
    4. transcription from RNA polymerase II promoter Source: Reactome
    5. transcription initiation from RNA polymerase II promoter Source: Reactome
    6. viral process Source: Reactome

    Keywords - Biological processi

    Host-virus interaction, Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_6233. Transcription of the HIV genome.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6332. HIV Transcription Initiation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    General transcription factor IIE subunit 1
    Alternative name(s):
    General transcription factor IIE 56 kDa subunit
    Transcription initiation factor IIE subunit alpha
    Short name:
    TFIIE-alpha
    Gene namesi
    Name:GTF2E1
    Synonyms:TF2E1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:4650. GTF2E1.

    Subcellular locationi

    GO - Cellular componenti

    1. intermediate filament cytoskeleton Source: HPA
    2. nucleoplasm Source: Reactome
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29036.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 439438General transcription factor IIE subunit 1PRO_0000211222Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine3 Publications
    Modified residuei67 – 671N6-acetyllysine1 Publication
    Modified residuei268 – 2681Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP29083.
    PaxDbiP29083.
    PeptideAtlasiP29083.
    PRIDEiP29083.

    PTM databases

    PhosphoSiteiP29083.

    Miscellaneous databases

    PMAP-CutDBP29083.

    Expressioni

    Gene expression databases

    ArrayExpressiP29083.
    BgeeiP29083.
    CleanExiHS_GTF2E1.
    GenevestigatoriP29083.

    Organism-specific databases

    HPAiCAB009884.
    HPA042437.

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta chains. Interacts with TAF6/TAFII80. Interacts with ATF7IP. Interacts with varicella-zoster virus IE63 protein.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GTF2E2P290842EBI-5462215,EBI-2853321
    GTF2H1P3278017EBI-5462215,EBI-715539

    Protein-protein interaction databases

    BioGridi109215. 47 interactions.
    DIPiDIP-717N.
    IntActiP29083. 3 interactions.
    MINTiMINT-1894058.
    STRINGi9606.ENSP00000283875.

    Structurei

    Secondary structure

    1
    439
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi118 – 1203
    Beta strandi123 – 1286
    Beta strandi130 – 1323
    Beta strandi135 – 1373
    Helixi138 – 1447
    Turni147 – 1504
    Beta strandi151 – 1533
    Beta strandi155 – 1573
    Beta strandi380 – 3834
    Beta strandi388 – 3903
    Beta strandi395 – 3984
    Beta strandi401 – 4044
    Helixi405 – 4106
    Helixi412 – 4176
    Helixi420 – 43314

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VD4NMR-A113-174[»]
    2JTXNMR-A336-439[»]
    2RNQNMR-A378-439[»]
    2RNRNMR-A378-439[»]
    ProteinModelPortaliP29083.
    SMRiP29083. Positions 113-174, 394-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP29083.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 10491HTH TFE/IIEalpha-typePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi378 – 39316Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the TFIIE alpha subunit family.Curated
    Contains 1 HTH TFE/IIEalpha-type domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri129 – 15729C4-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1675.
    HOGENOMiHOG000007666.
    HOVERGENiHBG008614.
    InParanoidiP29083.
    KOiK03136.
    OMAiLFQDMYF.
    PhylomeDBiP29083.
    TreeFamiTF313429.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR017919. TFIIE/TFIIEa_HTH.
    IPR002853. TFIIE_asu.
    IPR021600. TFIIE_asu_C.
    IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR013137. Znf_TFIIB.
    [Graphical view]
    PfamiPF08271. TF_Zn_Ribbon. 1 hit.
    PF11521. TFIIE-A_C-term. 1 hit.
    PF02002. TFIIE_alpha. 1 hit.
    [Graphical view]
    SMARTiSM00531. TFIIE. 1 hit.
    [Graphical view]
    PROSITEiPS51344. HTH_TFE_IIE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29083-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE    50
    LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV 100
    NVVKYKLDHM RRRIETDERD STNRASFKCP VCSSTFTDLE ANQLFDPMTG 150
    TFRCTFCHTE VEEDESAMPK KDARTLLARF NEQIEPIYAL LRETEDVNLA 200
    YEILEPEPTE IPALKQSKDH AATTAGAASL AGGHHREAWA TKGPSYEDLY 250
    TQNVVINMDD QEDLHRASLE GKSAKERPIW LRESTVQGAY GSEDMKEGGI 300
    DMDAFQEREE GHAGPDDNEE VMRALLIHEK KTSSAMAGSV GAAAPVTAAN 350
    GSDSESETSE SDDDSPPRPA AVAVHKREED EEEDDEFEEV ADDPIVMVAG 400
    RPFSYSEVSQ RPELVAQMTP EEKEAYIAMG QRMFEDLFE 439
    Length:439
    Mass (Da):49,452
    Last modified:October 17, 2006 - v2
    Checksum:i9234FFE3F150340B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti352 – 3521S → D in CAA45068. (PubMed:1956403)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti366 – 3661P → S.
    Corresponds to variant rs3732401 [ dbSNP | Ensembl ].
    VAR_020321

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S67859 mRNA. Translation: AAB20413.1.
    X63468 mRNA. Translation: CAA45068.1.
    CCDSiCCDS3002.1.
    PIRiS29291.
    RefSeqiNP_005504.2. NM_005513.2.
    UniGeneiHs.445272.

    Genome annotation databases

    EnsembliENST00000283875; ENSP00000283875; ENSG00000153767.
    GeneIDi2960.
    KEGGihsa:2960.
    UCSCiuc003edz.4. human.

    Polymorphism databases

    DMDMi116242812.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S67859 mRNA. Translation: AAB20413.1 .
    X63468 mRNA. Translation: CAA45068.1 .
    CCDSi CCDS3002.1.
    PIRi S29291.
    RefSeqi NP_005504.2. NM_005513.2.
    UniGenei Hs.445272.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VD4 NMR - A 113-174 [» ]
    2JTX NMR - A 336-439 [» ]
    2RNQ NMR - A 378-439 [» ]
    2RNR NMR - A 378-439 [» ]
    ProteinModelPortali P29083.
    SMRi P29083. Positions 113-174, 394-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109215. 47 interactions.
    DIPi DIP-717N.
    IntActi P29083. 3 interactions.
    MINTi MINT-1894058.
    STRINGi 9606.ENSP00000283875.

    PTM databases

    PhosphoSitei P29083.

    Polymorphism databases

    DMDMi 116242812.

    Proteomic databases

    MaxQBi P29083.
    PaxDbi P29083.
    PeptideAtlasi P29083.
    PRIDEi P29083.

    Protocols and materials databases

    DNASUi 2960.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000283875 ; ENSP00000283875 ; ENSG00000153767 .
    GeneIDi 2960.
    KEGGi hsa:2960.
    UCSCi uc003edz.4. human.

    Organism-specific databases

    CTDi 2960.
    GeneCardsi GC03P120461.
    HGNCi HGNC:4650. GTF2E1.
    HPAi CAB009884.
    HPA042437.
    MIMi 189962. gene.
    neXtProti NX_P29083.
    PharmGKBi PA29036.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1675.
    HOGENOMi HOG000007666.
    HOVERGENi HBG008614.
    InParanoidi P29083.
    KOi K03136.
    OMAi LFQDMYF.
    PhylomeDBi P29083.
    TreeFami TF313429.

    Enzyme and pathway databases

    Reactomei REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_6233. Transcription of the HIV genome.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6332. HIV Transcription Initiation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

    Miscellaneous databases

    ChiTaRSi GTF2E1. human.
    EvolutionaryTracei P29083.
    GeneWikii GTF2E1.
    GenomeRNAii 2960.
    NextBioi 11734.
    PMAP-CutDB P29083.
    PROi P29083.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P29083.
    Bgeei P29083.
    CleanExi HS_GTF2E1.
    Genevestigatori P29083.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR017919. TFIIE/TFIIEa_HTH.
    IPR002853. TFIIE_asu.
    IPR021600. TFIIE_asu_C.
    IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR013137. Znf_TFIIB.
    [Graphical view ]
    Pfami PF08271. TF_Zn_Ribbon. 1 hit.
    PF11521. TFIIE-A_C-term. 1 hit.
    PF02002. TFIIE_alpha. 1 hit.
    [Graphical view ]
    SMARTi SM00531. TFIIE. 1 hit.
    [Graphical view ]
    PROSITEi PS51344. HTH_TFE_IIE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and functional properties of human general transcription factor IIE."
      Peterson M.G., Inostroza J., Maxon M.E., Flores O., Admon A., Reinberg D., Tjian R.
      Nature 354:369-373(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE."
      Ohkuma Y., Sumimoto H., Hoffmann A., Shimasaki S., Horikoshi M., Roeder R.G.
      Nature 354:398-401(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    3. Bienvenut W.V.
      Submitted (JAN-2010) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-16; 154-170; 219-236; 243-266 AND 402-423, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Ovarian carcinoma.
    4. "Evolutionary conservation of human TATA-binding-polypeptide-associated factors TAFII31 and TAFII80 and interactions of TAFII80 with other TAFs and with general transcription factors."
      Hisatake K., Ohta T., Takada R., Guermah M., Horikoshi M., Nakatani Y., Roeder R.G.
      Proc. Natl. Acad. Sci. U.S.A. 92:8195-8199(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TAF6.
    5. "Varicella-zoster virus IE63 protein represses the basal transcription machinery by disorganizing the pre-initiation complex."
      Di Valentin E., Bontems S., Habran L., Jolois O., Markine-Goriaynoff N., Vanderplasschen A., Sadzot-Delvaux C., Piette J.
      Biol. Chem. 386:255-267(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VARICELLA-ZOSTER VIRUS IE63 PROTEIN.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity."
      Liu L., Ishihara K., Ichimura T., Fujita N., Hino S., Tomita S., Watanabe S., Saitoh N., Ito T., Nakao M.
      J. Biol. Chem. 284:5165-5174(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATF7IP.
    9. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiT2EA_HUMAN
    AccessioniPrimary (citable) accession number: P29083
    Secondary accession number(s): Q16103
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 143 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3