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P29083

- T2EA_HUMAN

UniProt

P29083 - T2EA_HUMAN

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Protein
General transcription factor IIE subunit 1
Gene
GTF2E1, TF2E1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri129 – 15729C4-type Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein binding Source: UniProtKB
  3. sequence-specific DNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. gene expression Source: Reactome
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription elongation from RNA polymerase II promoter Source: Reactome
  4. transcription from RNA polymerase II promoter Source: Reactome
  5. transcription initiation from RNA polymerase II promoter Source: Reactome
  6. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_6233. Transcription of the HIV genome.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6332. HIV Transcription Initiation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIE subunit 1
Alternative name(s):
General transcription factor IIE 56 kDa subunit
Transcription initiation factor IIE subunit alpha
Short name:
TFIIE-alpha
Gene namesi
Name:GTF2E1
Synonyms:TF2E1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:4650. GTF2E1.

Subcellular locationi

GO - Cellular componenti

  1. intermediate filament cytoskeleton Source: HPA
  2. nucleoplasm Source: Reactome
  3. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29036.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 439438General transcription factor IIE subunit 1
PRO_0000211222Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine3 Publications
Modified residuei67 – 671N6-acetyllysine1 Publication
Modified residuei268 – 2681Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP29083.
PaxDbiP29083.
PeptideAtlasiP29083.
PRIDEiP29083.

PTM databases

PhosphoSiteiP29083.

Miscellaneous databases

PMAP-CutDBP29083.

Expressioni

Gene expression databases

ArrayExpressiP29083.
BgeeiP29083.
CleanExiHS_GTF2E1.
GenevestigatoriP29083.

Organism-specific databases

HPAiCAB009884.
HPA042437.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Interacts with TAF6/TAFII80. Interacts with ATF7IP. Interacts with varicella-zoster virus IE63 protein.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GTF2E2P290842EBI-5462215,EBI-2853321
GTF2H1P3278017EBI-5462215,EBI-715539

Protein-protein interaction databases

BioGridi109215. 47 interactions.
DIPiDIP-717N.
IntActiP29083. 3 interactions.
MINTiMINT-1894058.
STRINGi9606.ENSP00000283875.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi118 – 1203
Beta strandi123 – 1286
Beta strandi130 – 1323
Beta strandi135 – 1373
Helixi138 – 1447
Turni147 – 1504
Beta strandi151 – 1533
Beta strandi155 – 1573
Beta strandi380 – 3834
Beta strandi388 – 3903
Beta strandi395 – 3984
Beta strandi401 – 4044
Helixi405 – 4106
Helixi412 – 4176
Helixi420 – 43314

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VD4NMR-A113-174[»]
2JTXNMR-A336-439[»]
2RNQNMR-A378-439[»]
2RNRNMR-A378-439[»]
ProteinModelPortaliP29083.
SMRiP29083. Positions 113-174, 394-439.

Miscellaneous databases

EvolutionaryTraceiP29083.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 10491HTH TFE/IIEalpha-type
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi378 – 39316Asp/Glu-rich (acidic)
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1675.
HOGENOMiHOG000007666.
HOVERGENiHBG008614.
InParanoidiP29083.
KOiK03136.
OMAiLFQDMYF.
PhylomeDBiP29083.
TreeFamiTF313429.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR021600. TFIIE_asu_C.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR013137. Znf_TFIIB.
[Graphical view]
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF11521. TFIIE-A_C-term. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view]
SMARTiSM00531. TFIIE. 1 hit.
[Graphical view]
PROSITEiPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29083-1 [UniParc]FASTAAdd to Basket

« Hide

MADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE    50
LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV 100
NVVKYKLDHM RRRIETDERD STNRASFKCP VCSSTFTDLE ANQLFDPMTG 150
TFRCTFCHTE VEEDESAMPK KDARTLLARF NEQIEPIYAL LRETEDVNLA 200
YEILEPEPTE IPALKQSKDH AATTAGAASL AGGHHREAWA TKGPSYEDLY 250
TQNVVINMDD QEDLHRASLE GKSAKERPIW LRESTVQGAY GSEDMKEGGI 300
DMDAFQEREE GHAGPDDNEE VMRALLIHEK KTSSAMAGSV GAAAPVTAAN 350
GSDSESETSE SDDDSPPRPA AVAVHKREED EEEDDEFEEV ADDPIVMVAG 400
RPFSYSEVSQ RPELVAQMTP EEKEAYIAMG QRMFEDLFE 439
Length:439
Mass (Da):49,452
Last modified:October 17, 2006 - v2
Checksum:i9234FFE3F150340B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti366 – 3661P → S.
Corresponds to variant rs3732401 [ dbSNP | Ensembl ].
VAR_020321

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3521S → D in CAA45068. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S67859 mRNA. Translation: AAB20413.1.
X63468 mRNA. Translation: CAA45068.1.
CCDSiCCDS3002.1.
PIRiS29291.
RefSeqiNP_005504.2. NM_005513.2.
UniGeneiHs.445272.

Genome annotation databases

EnsembliENST00000283875; ENSP00000283875; ENSG00000153767.
GeneIDi2960.
KEGGihsa:2960.
UCSCiuc003edz.4. human.

Polymorphism databases

DMDMi116242812.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S67859 mRNA. Translation: AAB20413.1 .
X63468 mRNA. Translation: CAA45068.1 .
CCDSi CCDS3002.1.
PIRi S29291.
RefSeqi NP_005504.2. NM_005513.2.
UniGenei Hs.445272.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VD4 NMR - A 113-174 [» ]
2JTX NMR - A 336-439 [» ]
2RNQ NMR - A 378-439 [» ]
2RNR NMR - A 378-439 [» ]
ProteinModelPortali P29083.
SMRi P29083. Positions 113-174, 394-439.
ModBasei Search...

Protein-protein interaction databases

BioGridi 109215. 47 interactions.
DIPi DIP-717N.
IntActi P29083. 3 interactions.
MINTi MINT-1894058.
STRINGi 9606.ENSP00000283875.

PTM databases

PhosphoSitei P29083.

Polymorphism databases

DMDMi 116242812.

Proteomic databases

MaxQBi P29083.
PaxDbi P29083.
PeptideAtlasi P29083.
PRIDEi P29083.

Protocols and materials databases

DNASUi 2960.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000283875 ; ENSP00000283875 ; ENSG00000153767 .
GeneIDi 2960.
KEGGi hsa:2960.
UCSCi uc003edz.4. human.

Organism-specific databases

CTDi 2960.
GeneCardsi GC03P120461.
HGNCi HGNC:4650. GTF2E1.
HPAi CAB009884.
HPA042437.
MIMi 189962. gene.
neXtProti NX_P29083.
PharmGKBi PA29036.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1675.
HOGENOMi HOG000007666.
HOVERGENi HBG008614.
InParanoidi P29083.
KOi K03136.
OMAi LFQDMYF.
PhylomeDBi P29083.
TreeFami TF313429.

Enzyme and pathway databases

Reactomei REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_6233. Transcription of the HIV genome.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6332. HIV Transcription Initiation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

ChiTaRSi GTF2E1. human.
EvolutionaryTracei P29083.
GeneWikii GTF2E1.
GenomeRNAii 2960.
NextBioi 11734.
PMAP-CutDB P29083.
PROi P29083.
SOURCEi Search...

Gene expression databases

ArrayExpressi P29083.
Bgeei P29083.
CleanExi HS_GTF2E1.
Genevestigatori P29083.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR021600. TFIIE_asu_C.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR013137. Znf_TFIIB.
[Graphical view ]
Pfami PF08271. TF_Zn_Ribbon. 1 hit.
PF11521. TFIIE-A_C-term. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view ]
SMARTi SM00531. TFIIE. 1 hit.
[Graphical view ]
PROSITEi PS51344. HTH_TFE_IIE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and functional properties of human general transcription factor IIE."
    Peterson M.G., Inostroza J., Maxon M.E., Flores O., Admon A., Reinberg D., Tjian R.
    Nature 354:369-373(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE."
    Ohkuma Y., Sumimoto H., Hoffmann A., Shimasaki S., Horikoshi M., Roeder R.G.
    Nature 354:398-401(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  3. Bienvenut W.V.
    Submitted (JAN-2010) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-16; 154-170; 219-236; 243-266 AND 402-423, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Ovarian carcinoma.
  4. "Evolutionary conservation of human TATA-binding-polypeptide-associated factors TAFII31 and TAFII80 and interactions of TAFII80 with other TAFs and with general transcription factors."
    Hisatake K., Ohta T., Takada R., Guermah M., Horikoshi M., Nakatani Y., Roeder R.G.
    Proc. Natl. Acad. Sci. U.S.A. 92:8195-8199(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAF6.
  5. "Varicella-zoster virus IE63 protein represses the basal transcription machinery by disorganizing the pre-initiation complex."
    Di Valentin E., Bontems S., Habran L., Jolois O., Markine-Goriaynoff N., Vanderplasschen A., Sadzot-Delvaux C., Piette J.
    Biol. Chem. 386:255-267(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VARICELLA-ZOSTER VIRUS IE63 PROTEIN.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity."
    Liu L., Ishihara K., Ichimura T., Fujita N., Hino S., Tomita S., Watanabe S., Saitoh N., Ito T., Nakao M.
    J. Biol. Chem. 284:5165-5174(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATF7IP.
  9. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiT2EA_HUMAN
AccessioniPrimary (citable) accession number: P29083
Secondary accession number(s): Q16103
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 17, 2006
Last modified: September 3, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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