P29074 (PTN4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 4
EC=3.1.3.48

Alternative name(s):
Protein-tyrosine phosphatase MEG1
Short name=MEG
Short name=PTPase-MEG1

Gene names

Name:

PTPN4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act at junctions between the membrane and the cytoskeleton.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm › cytoskeleton By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 FERM domain. Contains 1 PDZ (DHR) domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from direct assay PubMed 10940933. Source: UniProtKB cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell internal side of plasma membrane Inferred from direct assay PubMed 10940933. Source: UniProtKB
Molecular_function	non-membrane spanning protein tyrosine phosphatase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CRK	P46108	3	EBI-710431,EBI-886
KAT5	Q92993	2	EBI-710431,EBI-399080
NCK1	P16333	3	EBI-710431,EBI-389883

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 926

926

Tyrosine-protein phosphatase non-receptor type 4

PRO_0000219434

Regions

Domain

29 – 312

284

FERM

Domain

517 – 589

73

PDZ

Domain

655 – 911

257

Tyrosine-protein phosphatase

Region

852 – 858

7

Substrate binding By similarity

Sites

Active site

852

1

Phosphocysteine intermediate By similarity

Binding site

820

1

Substrate By similarity

Binding site

896

1

Substrate By similarity

Natural variations

Natural variant

924

1

T → S.
Corresponds to variant rs3189128 [ dbSNP | Ensembl ].

VAR_061033

Experimental info

Sequence conflict

131

1

K → E in BAG37355. Ref.2

Sequence conflict

772

1

G → K no nucleotide entry Ref.4

Sequence conflict

863

1

I → V in BAG37355. Ref.2

Secondary structure

1

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926

Helix Strand Turn

Details...

Beta strand

516 – 520

5

Beta strand

524 – 526

3

Beta strand

530 – 535

6

Helix

536 – 538

3

Beta strand

540 – 547

8

Beta strand

549 – 551

3

Helix

552 – 555

4

Beta strand

556 – 558

3

Beta strand

565 – 569

5

Helix

579 – 587

9

Helix

589 – 591

3

Beta strand

596 – 602

7

Helix

639 – 651

13

Helix

654 – 661

8

Turn

677 – 679

3

Helix

680 – 682

3

Helix

692 – 694

3

Beta strand

695 – 697

3

Beta strand

704 – 713

10

Beta strand

720 – 726

7

Helix

731 – 733

3

Helix

734 – 743

10

Beta strand

748 – 751

4

Beta strand

755 – 757

3

Beta strand

773 – 776

4

Beta strand

779 – 783

5

Beta strand

790 – 801

12

Turn

802 – 805

4

Beta strand

806 – 815

10

Beta strand

820 – 823

4

Helix

828 – 841

14

Beta strand

848 – 851

4

Beta strand

853 – 857

5

Helix

858 – 873

16

Helix

880 – 888

9

Helix

898 – 911

14

Sequences

	Sequence		Length	Mass (Da)
	P29074 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 4DAC6A87A675CFB0	FASTA	926	105,911
	10 20 30 40 50 60 MTSRFRLPAG RTYNVRASEL ARDRQHTEVV CNILLLDNTV QAFKVNKHDQ GQVLLDVVFK 70 80 90 100 110 120 HLDLTEQDYF GLQLADDSTD NPRWLDPNKP IRKQLKRGSP YSLNFRVKFF VSDPNKLQEE 130 140 150 160 170 180 YTRYQYFLQI KQDILTGRLP CPSNTAALLA SFAVQSELGD YDQSENLSGY LSDYSFIPNQ 190 200 210 220 230 240 PQDFEKEIAK LHQQHIGLSP AEAEFNYLNT ARTLELYGVE FHYARDQSNN EIMIGVMSGG 250 260 270 280 290 300 ILIYKNRVRM NTFPWLKIVK ISFKCKQFFI QLRKELHESR ETLLGFNMVN YRACKNLWKA 310 320 330 340 350 360 CVEHHTFFRL DRPLPPQKNF FAHYFTLGSK FRYCGRTEVQ SVQYGKEKAN KDRVFARSPS 370 380 390 400 410 420 KPLARKLMDW EVVSRNSISD DRLETQSLPS RSPPGTPNHR NSTFTQEGTR LRPSSVGHLV 430 440 450 460 470 480 DHMVHTSPSE VFVNQRSPSS TQANSIVLES SPSQETPGDG KPPALPPKQS KKNSWNQIHY 490 500 510 520 530 540 SHSQQDLESH INETFDIPSS PEKPTPNGGI PHDNLVLIRM KPDENGRFGF NVKGGYDQKM 550 560 570 580 590 600 PVIVSRVAPG TPADLCVPRL NEGDQVVLIN GRDIAEHTHD QVVLFIKASC ERHSGELMLL 610 620 630 640 650 660 VRPNAVYDVV EEKLENEPDF QYIPEKAPLD SVHQDDHSLR ESMIQLAEGL ITGTVLTQFD 670 680 690 700 710 720 QLYRKKPGMT MSCAKLPQNI SKNRYRDISP YDATRVILKG NEDYINANYI NMEIPSSSII 730 740 750 760 770 780 NQYIACQGPL PHTCTDFWQM TWEQGSSMVV MLTTQVERGR VKCHQYWPEP TGSSSYGCYQ 790 800 810 820 830 840 VTCHSEEGNT AYIFRKMTLF NQEKNESRPL TQIQYIAWPD HGVPDDSSDF LDFVCHVRNK 850 860 870 880 890 900 RAGKEEPVVV HCSAGIGRTG VLITMETAMC LIECNQPVYP LDIVRTMRDQ RAMMIQTPSQ 910 920 YRFVCEAILK VYEEGFVKPL TTSTNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, cloning, and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to cytoskeletal protein 4.1." Gu M., York J.D., Warshawsky I., Majerus P.W. Proc. Natl. Acad. Sci. U.S.A. 88:5867-5871(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[4]	"Identification of novel protein-tyrosine phosphatases in a human leukemia cell line, F-36P." Honda H., Shibuya M., Chiba S., Yazaki Y., Hirai H. Leukemia 7:742-746(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 745-858. Tissue: Leukemia.
[5]	"Solution structure of PDZ domain of protein tyrosine phosphatase, non-receptor type 4." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 507-612.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M68941 mRNA. Translation: AAA36530.1.
AK314836 mRNA. Translation: BAG37355.1.
BC010674 mRNA. Translation: AAH10674.1.

IPI

IPI00019949.

PIR

A41105.

RefSeq

NP_002821.1. NM_002830.3.

UniGene

Hs.469809.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CS5	NMR	-	A	507-612	[»]
2I75	X-ray	2.45	A	611-926	[»]
2VPH	X-ray	1.90	A/B	513-606	[»]
3NFK	X-ray	1.43	A/B	499-604	[»]
3NFL	X-ray	1.91	A/B/C/D	499-604	[»]

ProteinModelPortal

P29074.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-34634N.

IntAct

P29074. 12 interactions.

MINT

MINT-1367896.

STRING

9606.ENSP00000263708.

PTM databases

PhosphoSite

P29074.

Polymorphism databases

DMDM

131531.

Proteomic databases

PaxDb

P29074.

PeptideAtlas

P29074.

PRIDE

P29074.

Protocols and materials databases

DNASU

5775.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263708; ENSP00000263708; ENSG00000088179.

GeneID

5775.

KEGG

hsa:5775.

UCSC

uc002tmf.1. human.

Organism-specific databases

CTD

5775.

GeneCards

GC02P120612.

HGNC

HGNC:9656. PTPN4.

HPA

HPA019351.

MIM

176878. gene.

neXtProt

NX_P29074.

PharmGKB

PA34000.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5599.

HOGENOM

HOG000007048.

HOVERGEN

HBG008322.

InParanoid

P29074.

KO

K01104.

OMA

HTCTDFW.

OrthoDB

EOG42RD6J.

PhylomeDB

P29074.

Gene expression databases

ArrayExpress

P29074.

Bgee

P29074.

CleanEx

HS_PTPN4.

Genevestigator

P29074.

Family and domain databases

Gene3D

1.20.80.10. 1 hit.
2.30.29.30. 1 hit.

InterPro

IPR019749. Band_41_domain.
IPR019750. Band_41_fam.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR012151. Tyr_Pase_non-rcpt_typ-3/4.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]

Pfam

PF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00595. PDZ. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]

PIRSF

PIRSF000927. Tyr-Ptase_nr3. 1 hit.

PRINTS

PR00935. BAND41.
PR00700. PRTYPHPHTASE.

SMART

SM00295. B41. 1 hit.
SM00228. PDZ. 1 hit.
SM00194. PTPc. 1 hit.
[Graphical view]

SUPFAM

SSF47031. FERM_3-hlx. 1 hit.
SSF50156. PDZ. 1 hit.

PROSITE

PS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS50106. PDZ. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P29074.

ChEMBL

CHEMBL3165.

DrugBank

DB00630. Alendronate.

EvolutionaryTrace

P29074.

GenomeRNAi

5775.

NextBio

22462.

PMAP-CutDB

P29074.

SOURCE

Search...

Entry information

Entry name

PTN4_HUMAN

Accession

Primary (citable) accession number: P29074
Secondary accession number(s): B2RBV8, Q9UDA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.