Reviewed, UniProtKB/Swiss-Prot P29072 (CHEA_BACSU)

Names and origin

Protein names

Recommended name:
    Chemotaxis protein cheA
    EC=2.7.13.3

Gene names

Name:	cheA
Synonyms:	cheN
Ordered Locus Names:	BSU16430

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to either cheB or cheY.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Membrane Potential. Note: It is not known whether cheA is actually membrane bound although cheA has two regions at the C-terminal end with the potential to be transmembrane regions.
Sequence similarities	Contains 1 cheW-like domain. Contains 1 histidine kinase domain. Contains 1 HPt domain.

Ontologies

Keywords
Biological process	Chemotaxis Two-component regulatory system
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell motion Inferred from electronic annotation. Source: InterPro chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 672	672	Chemotaxis protein cheA		PRO_0000074711
Regions
	Domain	1 – 103	103	HPt
	Domain	290 – 540	251	Histidine kinase
	Domain	542 – 672	131	CheW-like
Amino acid modifications
	Modified residue	46	1	Phosphohistidine; by autocatalysis By similarity
Experimental info
	Sequence conflict	271 – 272	2	KQ → NE in AAA22313. Ref.1
	Sequence conflict	462	1	A → P in AAA22313. Ref.1
	Sequence conflict	466	1	Missing in AAA22313. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29072-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 7BC3456AC34CB999	FASTA	672	74,812
	10 20 30 40 50 60 MDMNQYLDVF IDESKEHLQT CNEKLLLLEK DPTDLQLVHD IFRAAHTLKG MSATMGYTDL 70 80 90 100 110 120 AHLTHLLENV LDAIRNGDME VTSDWLDILF EALDHLETMV QSIIDGGDGK RDISEVSAKL 130 140 150 160 170 180 DVNGAHAESA ASAEPAEAQS SASDWEYDEF ERTVIQEAEE QGFKRYEIKI SLNENCMLKA 190 200 210 220 230 240 VRVYMVFEKL NEVGEVAKTI PSAEVLETED FGTDFQVCFL THQSAEDIEQ LINGVSEIEH 250 260 270 280 290 300 VEVIQGAPLT SAEKPEESKQ EDSPAAAVPA KQEKQKQPAK NDEQAKHSAG GSKTIRVNID 310 320 330 340 350 360 RLDSLMNLFE ELVIDRGRLE QIAKELEHNE LTETVERMTR ISGDLQSIIL NMRMVPVETV 370 380 390 400 410 420 FNRFPRMIRQ LQKELNKKIE LSIIGAETEL DRTVIDEIGD PLVHLIRNSI DHGIEAPETR 430 440 450 460 470 480 LQKGKPESGK VVLKAYHSGN HVFIEVEDDG AGLNRKKILE KALERGVITE KEAETLEDNQ 490 500 510 520 530 540 IYELIFAPGF STADQISDIS GRGVGLDVVK NKLESLGGSV SVKSAEGQGS LFSIQLPLTL 550 560 570 580 590 600 SIISVLLIKL EEETFAIPIS SIIETAVIDR KDILQTHDRE VIDFRGHIVP VVYLKEEFKI 610 620 630 640 650 660 EDTRKDAEQL HIIVVKKGDK PTAFVVDSFI GQQEVVLKSL GDYLTNVFAI SGATILGDGE 670 VALIIDCNAL II

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacillus subtilis CheN, a homolog of CheA, the central regulator of chemotaxis in Escherichia coli." Fuhrer D.K., Ordal G.W. J. Bacteriol. 173:7443-7448(1991) [PubMed: 1938941] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 271-272; 462 AND 466.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M57894 Genomic DNA. Translation: AAA22313.1. AL009126 Genomic DNA. Translation: CAB13516.2.
PIR	QRBSCN. A41653.
RefSeq	NP_389525.2.
3D structure databases
HSSP	HSSP built from PDB template 1I59 based on UniProtKB Q56310.
ModBase	Search...
Protein-protein interaction databases
IntAct	P29072. 1 interaction.
Genome annotation databases
GeneID	939600.
GenomeReviews	Gene locus BSU16430 in contig AL009126_GR.
KEGG	bsu:BSU16430.
NMPDR	fig|224308.1.peg.1646.
Organism-specific databases
SubtiList	BG10256. cheA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P29072.
OMA	THDREVI.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1644-MON.
BRENDA	2.7.13.3. 150.
Family and domain databases
InterPro	IPR002545. CheW. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_P-trf. IPR004105. Sig_transdc_His_kin_subgr_dim. IPR010808. Sig_transdc_His_kinase_P2-bd. [Graphical view]
Pfam	PF01584. CheW. 1 hit. PF02895. H-kinase_dim. 1 hit. PF01627. Hpt. 1 hit. PF07194. P2. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
ProDom	PD003142. Hpt_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00260. CheW. 1 hit. SM00073. HPT. 1 hit. [Graphical view]
PROSITE	PS50851. CHEW. 1 hit. PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEA_BACSU

Accession

Primary (citable) accession number: P29072

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 16, 2009
Last modified:	November 3, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)