P29070 (CHS1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 1
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 1
Class-III chitin synthase 3

Gene names

Name:	chs-1
ORF Names:	B11H24.170, NCU03611

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class III subfamily.
Sequence caution	The sequence AAA33568.1 differs from that shown. Reason: Frameshift at positions 13, 24, 838 and 908.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 917	917	Chitin synthase 1		PRO_0000193701
Regions
	Topological domain	1 – 570	570	Extracellular Potential
	Transmembrane	571 – 591	21	Helical; Potential
	Topological domain	592 – 629	38	Cytoplasmic Potential
	Transmembrane	630 – 650	21	Helical; Potential
	Topological domain	651 – 664	14	Extracellular Potential
	Transmembrane	665 – 685	21	Helical; Potential
	Topological domain	686 – 716	31	Cytoplasmic Potential
	Transmembrane	717 – 737	21	Helical; Potential
	Topological domain	738 – 744	7	Extracellular Potential
	Transmembrane	745 – 765	21	Helical; Potential
	Topological domain	766 – 843	78	Cytoplasmic Potential
	Transmembrane	844 – 864	21	Helical; Potential
	Topological domain	865 – 884	20	Extracellular Potential
	Transmembrane	885 – 905	21	Helical; Potential
	Topological domain	906 – 917	12	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	544	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	33	1	A → R in AAA33568. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29070 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: 903EBBF6BD95A69A	FASTA	917	102,692
	10 20 30 40 50 60 MAYHGRGDGY DGHQLQDLPG GHNQGDQHDD AQAPFLSENP MPYDNDRLGT DTPPVRPVSA 70 80 90 100 110 120 YSLTESYAPG AGTTRAGVAV NPTPPPHGGY GGGGVSSGVD QGYNYGGDYA TDPAYRMSAI 130 140 150 160 170 180 DEDDSWLRRQ QPNAAPTGGL KRYATRKVKL VQGSVLSLDY PVPSAIRNAV QPKYRDEEGN 190 200 210 220 230 240 NEEFFKMRYT AATCDPNDFT LKNGYDLRPR MYNRHTELLI AITYYNEDKV LLSRTLHSVM 250 260 270 280 290 300 TNIRDIVNLK KSSFWNRGGP AWQKIVVCLV FDGLDKTDKN VLDVLATIGV YQDGVIKKDV 310 320 330 340 350 360 DGKETVAHIF EYTSQLSVTP NQALIRPVDD GPQTLPPVQF IFCLKQKNTK KINSHRWLFN 370 380 390 400 410 420 AFGRILNPEV CILLDAGTKP SPRSLLALWE GFYNDKDLGG ACGEIHAMLG KGGKKLLNPL 430 440 450 460 470 480 VAVQNFEYKI SNILDKPLES AFGYVSVLPG AFSAYRFRAI MGRPLEQYFH GDHTLSKLLG 490 500 510 520 530 540 KKGIEGMNIF KKNMFLAEDR ILCFELVAKA GQKWHLSYIK AAKGETDVPE GAPEFISQRR 550 560 570 580 590 600 RWLNGSFAAS LYSLMHFGRM YKSGHNIVRM FFFHVQLIYN IANVIFTWFS LASYWLTTTV 610 620 630 640 650 660 IMDLVGTPVT ASSSSAEHHG WPFGDTVTPF FNAVLKYIYL AFVILQFILA LGNRPKGSKW 670 680 690 700 710 720 TYITSFFVFS LIQSYILVLS GYLVARAFSV PLDQQLQLDN AKDAMASLFG GSGSAGVILV 730 740 750 760 770 780 ALVTIYGLYF LASFMYLDPW HMFHSFPYYM LLMSTYINIL MIYAFNNWHD VSWGTKGSDK 790 800 810 820 830 840 AEALPSANVS KGEKDEAVVE EIEKPQEDID QQFEATVRRA LAPYKEDETP EPKDLEDSYK 850 860 870 880 890 900 SFRTMLVVSW LFSNCLLAVV ITSDNFNTFG IGQTASARTA WFFKFLLFAT GALSVIRFIG 910 FCWFLGRTGI MCCFARR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chitin synthase 1 plays a major role in cell wall biogenesis in Neurospora crassa." Yarden O., Yanofsky C. Genes Dev. 5:2420-2430(1991) [PubMed: 1836444] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73437 Genomic DNA. Translation: AAA33568.1. Frameshift. AL670005 Genomic DNA. Translation: CAD21286.1. AABX02000011 Genomic DNA. Translation: EAA32102.2.
PIR	A41638.
RefSeq	XP_961338.2. XM_956245.2.
UniGene	Ncr.806.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000003325; EFNCRP00000003325; EFNCRG00000003321.
GeneID	3877520.
KEGG	ncr:NCU03611.
Phylogenomic databases
eggNOG	fuNOG07505.
GeneTree	EFGT00050000001909.
OMA	MHFGRMY.
OrthoDB	EOG405W8B.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
KO	K00698.
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS1_NEUCR

Accession

Primary (citable) accession number: P29070
Secondary accession number(s): Q7RVD4, Q8X0I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 7, 2005
Last modified:	December 14, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program