Reviewed, UniProtKB/Swiss-Prot P29061 (CHIB_TOBAC)

Names and origin

Protein names	Recommended name:     Basic endochitinase     EC=3.2.1.14
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein functions as a defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Induction	By TMV infection.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 294	270	Basic endochitinase		PRO_0000011919
Sites
	Active site	151	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	44 ↔ 91		By similarity
	Disulfide bond	74 ↔ 81		By similarity
	Disulfide bond	182 ↔ 211		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29061-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 5CE49A1455C70AE1	FASTA	294	32,393
	10 20 30 40 50 60 MNIKVSLLFI LPIFLLLLTS KVKAGDIVVY WGQDVGEGKL IDTCNSGLYN IVNIAFLSSF 70 80 90 100 110 120 GNFQTPKLNL AGHCEPSSGG CQQLTKSIRH CQSIGIKIML SIGGGTPTYT LSSVDDARQV 130 140 150 160 170 180 ADYLWNNFLG GQSSFRPLGD AVLDGIDFDI ELGQPHYIAL ARRLSEHGQQ GKKLYLTAAP 190 200 210 220 230 240 QCPFPDKLLN GALQTGLFDY VWVQFYNNPE CEFMSNSENF KRRWNQWTSI PAKKLYIGLP 250 260 270 280 290 AAKTAAGNGY IPKQVLMSQV LPFLKGSSKY GGVMLWNRKF DVQCGYSSAI RGAV

References

[1]	"Acidic and basic class III chitinase mRNA accumulation in response to TMV infection of tobacco." Lawton K., Ward E., Payne G., Moyer M., Ryals J. Plant Mol. Biol. 19:735-743(1992) [PubMed: 1643280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Xanthi. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11564 mRNA. Translation: CAA77657.1.
PIR	S23545.
3D structure databases
HSSP	HSSP built from PDB template 2HVM based on UniProtKB P23472.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 298.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIB_TOBAC

Accession

Primary (citable) accession number: P29061

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)