Reviewed, UniProtKB/Swiss-Prot P29060 (CHIA_TOBAC)

Names and origin

Protein names	Recommended name:     Acidic endochitinase     EC=3.2.1.14
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein functions as a defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › cell wall. Ref.2
Induction	By TMV infection.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.2
	Chain	23 – 291	269	Acidic endochitinase		PRO_0000011918
Sites
	Active site	149	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	42 ↔ 89		By similarity
	Disulfide bond	72 ↔ 79		By similarity
	Disulfide bond	180 ↔ 209		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29060-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: B843116D10DD14D3	FASTA	291	31,283
	10 20 30 40 50 60 MIKYSFLLTA LVLFLRALKL EAGDIVIYWG QNGNEGSLAD TCATNNYAIV NIAFLVVFGN 70 80 90 100 110 120 GQNPVLNLAG HCDPNAGACT GLSNDIRACQ NQGIKVMLSL GGGAGSYFLS SADDARNVAN 130 140 150 160 170 180 YLWNNYLGGQ SNTRPLGDAV LDGIDFDIEG GTTQHWDELA KTLSQFSQQR KVYLTAAPQC 190 200 210 220 230 240 PFPDTWLNGA LSTGLFDYVW VQFYNNPPCQ YSGGSADNLK NYWNQWNAIQ AGKIFLGLPA 250 260 270 280 290 AQGAAGSGFI PSDVLVSQVL PLINGSPKYG GVMLWSKFYD NGYSSAIKAN V

References

[1]

"Acidic and basic class III chitinase mRNA accumulation in response to TMV infection of tobacco." Lawton K., Ward E., Payne G., Moyer M., Ryals J. Plant Mol. Biol. 19:735-743(1992) [PubMed: 1643280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Xanthi. Tissue: Leaf.

[2]

"Proteomic analysis reveals a novel set of cell wall proteins in a transformed tobacco cell culture that synthesises secondary walls as determined by biochemical and morphological parameters." Blee K.A., Wheatley E.R., Bonham V.A., Mitchell G.P., Robertson D., Slabas A.R., Burrell M.M., Wojtaszek P., Bolwell G.P. Planta 212:404-415(2001) [PubMed: 11289605] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-36, SUBCELLULAR LOCATION. Strain: cv. Petit Havana.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11563 mRNA. Translation: CAA77656.1.
PIR	S23544.
3D structure databases
HSSP	HSSP built from PDB template 2HVM based on UniProtKB P23472.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 298.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_TOBAC

Accession

Primary (citable) accession number: P29060
Secondary accession number(s): P82433

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	October 13, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)