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Protein

Endochitinase 3

Gene

CHN14

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Defense against chitin-containing fungal pathogens.

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase 3 (EC:3.2.1.14)
Gene namesi
Name:CHN14
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
Proteomesi
  • UP000084051 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000000533424 – 327Endochitinase 3Add BLAST304
PropeptideiPRO_0000005335328 – 334Removed in mature formCurated7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 41PROSITE-ProRule annotation
Disulfide bondi35 ↔ 47PROSITE-ProRule annotation
Disulfide bondi40 ↔ 54PROSITE-ProRule annotation
Disulfide bondi59 ↔ 63PROSITE-ProRule annotation
Modified residuei734-hydroxyprolineBy similarity1
Modified residuei744-hydroxyprolineBy similarity1
Modified residuei764-hydroxyprolineBy similarity1
Disulfide bondi106 ↔ 168PROSITE-ProRule annotation
Disulfide bondi180 ↔ 188PROSITE-ProRule annotation
Disulfide bondi287 ↔ 319PROSITE-ProRule annotation

Post-translational modificationi

The 4-hydroxyproline residues are not glycosylated in this plant vacuolar protein.By similarity

Keywords - PTMi

Disulfide bond, Hydroxylation

Proteomic databases

PRIDEiP29059.

Structurei

3D structure databases

ProteinModelPortaliP29059.
SMRiP29059.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 65Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST42

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20547.

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
Gene3Di3.30.60.10. 1 hit.
InterProiView protein in InterPro
IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR036861. Endochitinase-like_sf.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom_sf.
PfamiView protein in Pfam
PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609. Chitin_bd_1. 1 hit.
SMARTiView protein in SMART
SM00270. ChtBD1. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiView protein in PROSITE
PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLEFTALS SLLVLFLLLA VSAEQCGKQA GGARCPSGMC CSNFGWCGNT
60 70 80 90 100
QDYCGPGKCQ SQCPSGPGPT PRPPTPTPGP STGDISNIIS SSMFDQMLKH
110 120 130 140 150
RNDNTCQGKS FYTYNAFITA ARSFRGFGTT GDTTRRKREV AAFFAQTSHE
160 170 180 190 200
TTGGWDTAPD GRYAWGYCYL REQGNPPSYC VQSSQWPCAP GQKYYGRGPI
210 220 230 240 250
QISYNYNYGP CGRAIGQNLL NNPDLVATNA VVSFKSAIWF WMTAQSPKPS
260 270 280 290 300
CHDVITGRWT PSAADRAANR LPGYGVITNI INGGLECGHG SDARVQDRIG
310 320 330
FYRRYCSILG VSPGDNIDCG NQKSFNSGLL LETM
Length:334
Mass (Da):36,221
Last modified:December 1, 1992 - v1
Checksum:i4F684CE1FBD432FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64518 Genomic DNA. Translation: CAA45821.1.
PIRiS20982.
RefSeqiXP_016461053.1. XM_016605567.1.

Genome annotation databases

GeneIDi107784434.
KEGGinta:107784434.

Similar proteinsi

Entry informationi

Entry nameiCHI3_TOBAC
AccessioniPrimary (citable) accession number: P29059
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 22, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families