Reviewed, UniProtKB/Swiss-Prot P29059 (CHI3_TOBAC)

Names and origin

Protein names

Recommended name:
    Endochitinase 3
    EC=3.2.1.14

Gene names

Name:

CHN14

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Vacuole Probable. Note: Vacuolar and protoplast Probable.
Post-translational modification	The 4-hydroxyproline residues are not glycosylated in this plant vacuolar protein By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Vacuole
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Hydroxylation
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 327	304	Endochitinase 3		PRO_0000005334
	Propeptide	328 – 334	7	Removed in mature form Probable		PRO_0000005335
Regions
	Domain	24 – 65	42	Chitin-binding type-1
Amino acid modifications
	Modified residue	73	1	4-hydroxyproline By similarity
	Modified residue	74	1	4-hydroxyproline By similarity
	Modified residue	76	1	4-hydroxyproline By similarity
	Disulfide bond	26 ↔ 41		By similarity
	Disulfide bond	35 ↔ 47		By similarity
	Disulfide bond	40 ↔ 54		By similarity
	Disulfide bond	59 ↔ 63		By similarity
	Disulfide bond	106 ↔ 168		By similarity
	Disulfide bond	180 ↔ 188		By similarity
	Disulfide bond	287 ↔ 319		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29059-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 4F684CE1FBD432FB	FASTA	334	36,221
	10 20 30 40 50 60 MRLLEFTALS SLLVLFLLLA VSAEQCGKQA GGARCPSGMC CSNFGWCGNT QDYCGPGKCQ 70 80 90 100 110 120 SQCPSGPGPT PRPPTPTPGP STGDISNIIS SSMFDQMLKH RNDNTCQGKS FYTYNAFITA 130 140 150 160 170 180 ARSFRGFGTT GDTTRRKREV AAFFAQTSHE TTGGWDTAPD GRYAWGYCYL REQGNPPSYC 190 200 210 220 230 240 VQSSQWPCAP GQKYYGRGPI QISYNYNYGP CGRAIGQNLL NNPDLVATNA VVSFKSAIWF 250 260 270 280 290 300 WMTAQSPKPS CHDVITGRWT PSAADRAANR LPGYGVITNI INGGLECGHG SDARVQDRIG 310 320 330 FYRRYCSILG VSPGDNIDCG NQKSFNSGLL LETM

References

[1]

"The structure and regulation of homeologous tobacco endochitinase genes of Nicotiana sylvestris and N. tomentosiformis origin." van Buuren M., Neuhaus J.-M., Shinshi H., Ryals J., Meins F. Jr. Mol. Gen. Genet. 232:460-469(1992) [PubMed: 1588915] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Havana 425. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64518 Genomic DNA. Translation: CAA45821.1.
PIR	S20982.
3D structure databases
SMR	P29059. Positions 24-326.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 298.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI3_TOBAC

Accession

Primary (citable) accession number: P29059

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 19, 2010
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)