P29058 (HMDH2_HEVBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Short name=HMG-CoA reductase 2
EC=1.1.1.34

Gene names

Name:

HMGR2

Organism

Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)

Taxonomic identifier

3981 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Euphorbiaceae › Crotonoideae › Micrandreae › Hevea

Protein attributes

Sequence length	210 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Plastid membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Plastid
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 210	›210	3-hydroxy-3-methylglutaryl-coenzyme A reductase 2		PRO_0000114441
Regions
	Transmembrane	166 – 186	21	Helical; Potential
Sites
	Active site	21	1	Charge relay system By similarity
	Active site	97	1	Charge relay system By similarity
	Active site	195	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	199	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P29058 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 059C3C30766BD911	FASTA	210	21,564
	10 20 30 40 50 60 LESDFADMDV IGISGNFCSD KKPAAVNWIE GRGKSVVCEA IIKEEVVKKV LKTDVALLVE 70 80 90 100 110 120 LNMLKNLAGS AVAGALGGFN AHAGNIVSAI FIATGQDPAQ NVESSHCITM MEAVNDGKDL 130 140 150 160 170 180 HISVTLPSIE VGTVGGGTQL ASQSACLNLL GVMGACKESP GSYSRLLATI VAGSVLAGEL 190 200 210 SLMSAIAAGQ LVKSHMKYNR SSKDVSKAAS

References

[1]	"Characterization of cDNA and genomic clones encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase from Hevea brasiliensis." Chye M.L., Kush A., Tan C.T., Chua N.H. Plant Mol. Biol. 16:567-577(1991) [PubMed: 1714317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. RRIM 600. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54658 mRNA. Translation: CAA38468.1.
PIR	S14953.
3D structure databases
ProteinModelPortal	P29058.
SMR	P29058. Positions 1-201.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. Partial match. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH2_HEVBR

Accession

Primary (citable) accession number: P29058

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 28, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program