Reviewed, UniProtKB/Swiss-Prot P29057 (HMDH1_HEVBR)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
      Short name=HMG-CoA reductase 1
    EC=1.1.1.34

Gene names

Name:

HMGR1

Organism

Hevea brasiliensis (Para rubber tree)

Taxonomic identifier

3981 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Euphorbiaceae › Crotonoideae › Micrandreae › Hevea

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Plastid membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Plastid
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW plastid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1		PRO_0000114440
Regions
	Transmembrane	29 – 49	21	Potential
	Transmembrane	73 – 93	21	Potential
	Transmembrane	531 – 551	21	Potential
	Region	97 – 160	64	Linker By similarity
	Region	161 – 575	415	Catalytic By similarity
Sites
	Active site	254	1	Charge relay system By similarity
	Active site	386	1	Charge relay system By similarity
	Active site	462	1	Charge relay system By similarity
	Active site	560	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	132	1	N-linked (GlcNAc...) Potential
	Glycosylation	318	1	N-linked (GlcNAc...) Potential
	Glycosylation	564	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P29057-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: BC0A74898B6124D5	FASTA	575	61,695
	10 20 30 40 50 60 MDTTGRLHHR KHATPVEDRS PTTPKASDAL PLPLYLTNAV FFTLFFSVAY YLLHRWRDKI 70 80 90 100 110 120 RNSTPLHIVT LSEIVAIVSL IASFIYLLGF FGIDFVQSFI ARASHDVWDL EDTDPNYLID 130 140 150 160 170 180 EDHRLVTCPP ANISTKTTII AAPTKLPTSE PLIAPLVSEE DEMIVNSVVD GKIPSYSLES 190 200 210 220 230 240 KLGDCKRAAA IRREALQRMT RRSLEGLPVE GFDYESILGQ CCEMPVGYVQ IPVGIAGPLL 250 260 270 280 290 300 LNGREYSVPM ATTEGCLVAS TNRGCKAIYL SGGATSVLLK DGMTRAPVVR FASATRAAEL 310 320 330 340 350 360 KFFLEDPDNF DTLAVVFNKS SRFARLQGIK CSIAGKNLYI RFSCSTGDAM GMNMVSKGVQ 370 380 390 400 410 420 NVLEFLQSDF SDMDVIGISG NFCSDKKPAA VNWIEGRGKS VVCEAIIKEE VVKKVLKTNV 430 440 450 460 470 480 ASLVELNMLK NLAGSAVAGA LGGFNAHAGN IVSAIFIATG QDPAQNVESS HCITMMEAVN 490 500 510 520 530 540 DGKDLHISVT MPSIEVGTVG GGTQLASQSA CLNLLGVKGA NKESPGSNSR LLAAIVAGSV 550 560 570 LAGELSLMSA IAAGQLVKSH MKYNRSSKDM SKAAS

References

[1]	"Characterization of cDNA and genomic clones encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase from Hevea brasiliensis." Chye M.L., Kush A., Tan C.T., Chua N.H. Plant Mol. Biol. 16:567-577(1991) [PubMed: 1714317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. RRIM 600. Tissue: Leaf.
[2]	"Cloning and nucleotide sequence analysis of HMGR1 gene in Hevea brasiliensis." Venkatachalam P., Priya P., Thulaseedharan A. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54657 Genomic DNA. Translation: CAA38467.1. X54659 mRNA. Translation: CAA38469.1. AY352338 Genomic DNA. Translation: AAQ63055.1.
PIR	S14955.
3D structure databases
HSSP	HSSP built from PDB template 1DQA based on UniProtKB P04035.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.34. 2218.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH1_HEVBR

Accession

Primary (citable) accession number: P29057

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	October 13, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)