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Protein

Transcription factor IIIB 70 kDa subunit

Gene

BRF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.

Miscellaneous

Present with 4330 molecules/cell in log phase SD medium.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi4ZincBy similarity1
Metal bindingi7ZincBy similarity1
Metal bindingi25ZincBy similarity1
Metal bindingi28ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 33TFIIB-typePROSITE-ProRule annotationAdd BLAST33

GO - Molecular functioni

  • DNA binding Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase III core binding Source: SGD
  • TBP-class protein binding Source: InterPro
  • TFIIIB-type transcription factor activity Source: SGD
  • TFIIIC-class transcription factor binding Source: SGD

GO - Biological processi

  • DNA-templated transcriptional open complex formation Source: SGD
  • negative regulation of protein homodimerization activity Source: SGD
  • RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  • transposon integration Source: SGD

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30921-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 70 kDa subunit
Short name:
TFIIIB
Alternative name(s):
B-related factor 1
Short name:
BRF-1
Gene namesi
Name:BRF1
Synonyms:PCF4, TDS4
Ordered Locus Names:YGR246C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR246C
SGDiS000003478 BRF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001193461 – 596Transcription factor IIIB 70 kDa subunitAdd BLAST596

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei381PhosphoserineCombined sources1
Modified residuei384PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP29056
PaxDbiP29056
PRIDEiP29056

PTM databases

iPTMnetiP29056

Interactioni

Subunit structurei

TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and the B" component (TFC5).

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT15P133932EBI-19142,EBI-19129

GO - Molecular functioni

  • RNA polymerase III core binding Source: SGD
  • TBP-class protein binding Source: InterPro
  • TFIIIC-class transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi33497, 41 interactors
DIPiDIP-191N
IntActiP29056, 13 interactors
MINTiP29056
STRINGi4932.YGR246C

Structurei

Secondary structure

1596
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi442 – 444Combined sources3
Helixi448 – 452Combined sources5
Helixi470 – 472Combined sources3
Helixi477 – 490Combined sources14
Turni491 – 493Combined sources3
Helixi494 – 500Combined sources7
Turni501 – 505Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95B/F/J/N437-506[»]
6EU0electron microscopy4.00Z1-596[»]
6F40electron microscopy3.70V1-596[»]
6F41electron microscopy4.30V1-596[»]
6F42electron microscopy5.50V1-596[»]
6F44electron microscopy4.20V1-596[»]
ProteinModelPortaliP29056
SMRiP29056
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29056

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati90 – 1661Add BLAST77
Repeati185 – 2642Add BLAST80

Sequence similaritiesi

Belongs to the TFIIB family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 33TFIIB-typePROSITE-ProRule annotationAdd BLAST33

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000010349
HOGENOMiHOG000198688
InParanoidiP29056
KOiK15196
OMAiHDPPSFY
OrthoDBiEOG092C14D8

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR029529 Brf1
IPR011665 BRF1_TBP-bd_dom
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR000812 TFIIB
IPR023486 TFIIB_CS
IPR013150 TFIIB_cyclin
IPR013137 Znf_TFIIB
PANTHERiPTHR11618 PTHR11618, 1 hit
PTHR11618:SF4 PTHR11618:SF4, 1 hit
PfamiView protein in Pfam
PF07741 BRF1, 1 hit
PF08271 TF_Zn_Ribbon, 1 hit
PF00382 TFIIB, 2 hits
PRINTSiPR00685 TIFACTORIIB
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00782 TFIIB, 2 hits
PS51134 ZF_TFIIB, 1 hit

Sequencei

Sequence statusi: Complete.

P29056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA
60 70 80 90 100
AVVQGSFIGA GQSHAAFGGS SALESREATL NNARRKLRAV SYALHIPEYI
110 120 130 140 150
TDAAFQWYKL ALANNFVQGR RSQNVIASCL YVACRKEKTH HMLIDFSSRL
160 170 180 190 200
QVSVYSIGAT FLKMVKKLHI TELPLADPSL FIQHFAEKLD LADKKIKVVK
210 220 230 240 250
DAVKLAQRMS KDWMFEGRRP AGIAGACILL ACRMNNLRRT HTEIVAVSHV
260 270 280 290 300
AEETLQQRLN EFKNTKAAKL SVQKFRENDV EDGEARPPSF VKNRKKERKI
310 320 330 340 350
KDSLDKEEMF QTSEEALNKN PILTQVLGEQ ELSSKEVLFY LKQFSERRAR
360 370 380 390 400
VVERIKATNG IDGENIYHEG SENETRKRKL SEVSIQNEHV EGEDKETEGT
410 420 430 440 450
EEKVKKVKTK TSEEKKENES GHFQDAIDGY SLETDPYCPR NLHLLPTTDT
460 470 480 490 500
YLSKVSDDPD NLEDVDDEEL NAHLLNEEAS KLKERIWIGL NADFLLEQES
510 520 530 540 550
KRLKQEADIA TGNTSVKKKR TRRRNNTRSD EPTKTVDAAA AIGLMSDLQD
560 570 580 590
KSGLHAALKA AEESGDFTTA DSVKNMLQKA SFSKKINYDA IDGLFR
Length:596
Mass (Da):66,906
Last modified:December 1, 1992 - v1
Checksum:i489207643A66B796
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91073 Genomic DNA Translation: AAB04945.1
L00630 Genomic DNA Translation: AAA35148.1
Y07703 Genomic DNA Translation: CAA68968.1
Z73031 Genomic DNA Translation: CAA97275.1
BK006941 Genomic DNA Translation: DAA08337.1
PIRiA44072
RefSeqiNP_011762.1, NM_001181375.1

Genome annotation databases

EnsemblFungiiYGR246C; YGR246C; YGR246C
GeneIDi853161
KEGGisce:YGR246C

Similar proteinsi

Entry informationi

Entry nameiTF3B_YEAST
AccessioniPrimary (citable) accession number: P29056
Secondary accession number(s): D6VV26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 23, 2018
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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