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Protein

Transcription factor IIIB 70 kDa subunit

Gene

BRF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi4ZincBy similarity1
Metal bindingi7ZincBy similarity1
Metal bindingi25ZincBy similarity1
Metal bindingi28ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 33TFIIB-typePROSITE-ProRule annotationAdd BLAST33

GO - Molecular functioni

  • DNA binding Source: SGD
  • RNA polymerase III core binding Source: SGD
  • TFIIIB-type transcription factor activity Source: SGD
  • TFIIIC-class transcription factor binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • DNA-templated transcriptional open complex formation Source: SGD
  • negative regulation of protein homodimerization activity Source: SGD
  • RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  • transcription elongation from RNA polymerase III promoter Source: Reactome
  • transcription from RNA polymerase III promoter Source: Reactome
  • transposon integration Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30921-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 70 kDa subunit
Short name:
TFIIIB
Alternative name(s):
B-related factor 1
Short name:
BRF-1
Gene namesi
Name:BRF1
Synonyms:PCF4, TDS4
Ordered Locus Names:YGR246C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR246C.
SGDiS000003478. BRF1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • transcription factor TFIIIB complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001193461 – 596Transcription factor IIIB 70 kDa subunitAdd BLAST596

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei381PhosphoserineCombined sources1
Modified residuei384PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP29056.
PRIDEiP29056.

PTM databases

iPTMnetiP29056.

Interactioni

Subunit structurei

TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and the B" component (TFC5).

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT15P133932EBI-19142,EBI-19129

GO - Molecular functioni

  • RNA polymerase III core binding Source: SGD
  • TFIIIC-class transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi33497. 38 interactors.
DIPiDIP-191N.
IntActiP29056. 13 interactors.
MINTiMINT-2783113.

Structurei

Secondary structure

1596
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi442 – 444Combined sources3
Helixi448 – 452Combined sources5
Helixi470 – 472Combined sources3
Helixi477 – 490Combined sources14
Turni491 – 493Combined sources3
Helixi494 – 500Combined sources7
Turni501 – 505Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95B/F/J/N437-506[»]
ProteinModelPortaliP29056.
SMRiP29056.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29056.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati90 – 1661Add BLAST77
Repeati185 – 2642Add BLAST80

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 33TFIIB-typePROSITE-ProRule annotationAdd BLAST33

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000010349.
HOGENOMiHOG000198688.
InParanoidiP29056.
KOiK15196.
OMAiCRKERTH.
OrthoDBiEOG092C14D8.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR029529. Brf1.
IPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 2 hits.
PTHR11618:SF4. PTHR11618:SF4. 2 hits.
PfamiPF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA
60 70 80 90 100
AVVQGSFIGA GQSHAAFGGS SALESREATL NNARRKLRAV SYALHIPEYI
110 120 130 140 150
TDAAFQWYKL ALANNFVQGR RSQNVIASCL YVACRKEKTH HMLIDFSSRL
160 170 180 190 200
QVSVYSIGAT FLKMVKKLHI TELPLADPSL FIQHFAEKLD LADKKIKVVK
210 220 230 240 250
DAVKLAQRMS KDWMFEGRRP AGIAGACILL ACRMNNLRRT HTEIVAVSHV
260 270 280 290 300
AEETLQQRLN EFKNTKAAKL SVQKFRENDV EDGEARPPSF VKNRKKERKI
310 320 330 340 350
KDSLDKEEMF QTSEEALNKN PILTQVLGEQ ELSSKEVLFY LKQFSERRAR
360 370 380 390 400
VVERIKATNG IDGENIYHEG SENETRKRKL SEVSIQNEHV EGEDKETEGT
410 420 430 440 450
EEKVKKVKTK TSEEKKENES GHFQDAIDGY SLETDPYCPR NLHLLPTTDT
460 470 480 490 500
YLSKVSDDPD NLEDVDDEEL NAHLLNEEAS KLKERIWIGL NADFLLEQES
510 520 530 540 550
KRLKQEADIA TGNTSVKKKR TRRRNNTRSD EPTKTVDAAA AIGLMSDLQD
560 570 580 590
KSGLHAALKA AEESGDFTTA DSVKNMLQKA SFSKKINYDA IDGLFR
Length:596
Mass (Da):66,906
Last modified:December 1, 1992 - v1
Checksum:i489207643A66B796
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91073 Genomic DNA. Translation: AAB04945.1.
L00630 Genomic DNA. Translation: AAA35148.1.
Y07703 Genomic DNA. Translation: CAA68968.1.
Z73031 Genomic DNA. Translation: CAA97275.1.
BK006941 Genomic DNA. Translation: DAA08337.1.
PIRiA44072.
RefSeqiNP_011762.1. NM_001181375.1.

Genome annotation databases

EnsemblFungiiYGR246C; YGR246C; YGR246C.
GeneIDi853161.
KEGGisce:YGR246C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91073 Genomic DNA. Translation: AAB04945.1.
L00630 Genomic DNA. Translation: AAA35148.1.
Y07703 Genomic DNA. Translation: CAA68968.1.
Z73031 Genomic DNA. Translation: CAA97275.1.
BK006941 Genomic DNA. Translation: DAA08337.1.
PIRiA44072.
RefSeqiNP_011762.1. NM_001181375.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95B/F/J/N437-506[»]
ProteinModelPortaliP29056.
SMRiP29056.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33497. 38 interactors.
DIPiDIP-191N.
IntActiP29056. 13 interactors.
MINTiMINT-2783113.

PTM databases

iPTMnetiP29056.

Proteomic databases

MaxQBiP29056.
PRIDEiP29056.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR246C; YGR246C; YGR246C.
GeneIDi853161.
KEGGisce:YGR246C.

Organism-specific databases

EuPathDBiFungiDB:YGR246C.
SGDiS000003478. BRF1.

Phylogenomic databases

GeneTreeiENSGT00390000010349.
HOGENOMiHOG000198688.
InParanoidiP29056.
KOiK15196.
OMAiCRKERTH.
OrthoDBiEOG092C14D8.

Enzyme and pathway databases

BioCyciYEAST:G3O-30921-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

EvolutionaryTraceiP29056.
PROiP29056.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR029529. Brf1.
IPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 2 hits.
PTHR11618:SF4. PTHR11618:SF4. 2 hits.
PfamiPF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF3B_YEAST
AccessioniPrimary (citable) accession number: P29056
Secondary accession number(s): D6VV26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.