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P29056 (TF3B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor IIIB 70 kDa subunit

Short name=TFIIIB
Alternative name(s):
B-related factor 1
Short name=BRF-1
Gene names
Name:BRF1
Synonyms:PCF4, TDS4
Ordered Locus Names:YGR246C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length596 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and the B" component (TFC5).

Subcellular location

Nucleus.

Miscellaneous

Present with 4330 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TFIIB family.

Contains 1 TFIIB-type zinc finger.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionActivator
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA-templated transcriptional open complex formation

Inferred from mutant phenotype PubMed 11387215. Source: SGD

RNA polymerase III transcriptional preinitiation complex assembly

Inferred from direct assay PubMed 11387215. Source: SGD

negative regulation of protein homodimerization activity

Inferred from direct assay PubMed 15190063. Source: SGD

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

regulation of transcription from RNA polymerase III promoter

Inferred by curator PubMed 11387215PubMed 12930823PubMed 1458536PubMed 7995525. Source: GOC

transposon integration

Inferred from direct assay PubMed 10882723. Source: SGD

   Cellular_componentnucleoplasm

Traceable author statement. Source: Reactome

transcription factor TFIIIB complex

Inferred from direct assay Ref.2PubMed 1458536. Source: SGD

   Molecular_functionDNA binding

Inferred from direct assay PubMed 9271407. Source: SGD

RNA polymerase III core binding

Inferred from direct assay PubMed 7995525PubMed 9689057. Source: SGD

TFIIIB-type transcription factor activity

Inferred by curator PubMed 11387215PubMed 12930823PubMed 1458536PubMed 7995525. Source: SGD

TFIIIC-class transcription factor binding

Inferred from direct assay PubMed 12930823. Source: SGD

protein binding

Inferred from physical interaction PubMed 10788514PubMed 8340360. Source: IntAct

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SPT15P133932EBI-19142,EBI-19129

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 596596Transcription factor IIIB 70 kDa subunit
PRO_0000119346

Regions

Repeat90 – 166771
Repeat185 – 264802
Zinc finger1 – 3333TFIIB-type

Sites

Metal binding41Zinc By similarity
Metal binding71Zinc By similarity
Metal binding251Zinc By similarity
Metal binding281Zinc By similarity

Amino acid modifications

Modified residue3811Phosphoserine Ref.8 Ref.9
Modified residue3841Phosphoserine Ref.8

Secondary structure

............ 596
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29056 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 489207643A66B796

FASTA59666,906
        10         20         30         40         50         60 
MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA AVVQGSFIGA 

        70         80         90        100        110        120 
GQSHAAFGGS SALESREATL NNARRKLRAV SYALHIPEYI TDAAFQWYKL ALANNFVQGR 

       130        140        150        160        170        180 
RSQNVIASCL YVACRKEKTH HMLIDFSSRL QVSVYSIGAT FLKMVKKLHI TELPLADPSL 

       190        200        210        220        230        240 
FIQHFAEKLD LADKKIKVVK DAVKLAQRMS KDWMFEGRRP AGIAGACILL ACRMNNLRRT 

       250        260        270        280        290        300 
HTEIVAVSHV AEETLQQRLN EFKNTKAAKL SVQKFRENDV EDGEARPPSF VKNRKKERKI 

       310        320        330        340        350        360 
KDSLDKEEMF QTSEEALNKN PILTQVLGEQ ELSSKEVLFY LKQFSERRAR VVERIKATNG 

       370        380        390        400        410        420 
IDGENIYHEG SENETRKRKL SEVSIQNEHV EGEDKETEGT EEKVKKVKTK TSEEKKENES 

       430        440        450        460        470        480 
GHFQDAIDGY SLETDPYCPR NLHLLPTTDT YLSKVSDDPD NLEDVDDEEL NAHLLNEEAS 

       490        500        510        520        530        540 
KLKERIWIGL NADFLLEQES KRLKQEADIA TGNTSVKKKR TRRRNNTRSD EPTKTVDAAA 

       550        560        570        580        590 
AIGLMSDLQD KSGLHAALKA AEESGDFTTA DSVKNMLQKA SFSKKINYDA IDGLFR 

« Hide

References

« Hide 'large scale' references
[1]"PCF4 encodes an RNA polymerase III transcription factor with homology to TFIIB."
Lopez-De-Leon A., Librizzi M., Puglia K., Willis I.M.
Cell 71:211-220(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB."
Buratowski S., Zhou H.
Cell 71:221-230(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A yeast TFIIB-related factor involved in RNA polymerase III transcription."
Colbert T., Hahn S.
Genes Dev. 6:1940-1949(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII reveals the presence of eight open reading frames, including BRF1 (TFIIIB70) and GCN5 genes."
Feroli F., Carignani G., Pavanello A., Guerreiro P., Azevedo D., Rodrigues-Pousada C., Melchioretto P., Panzeri L., Agostoni Carbone M.L.
Yeast 13:373-377(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381 AND SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M91073 Genomic DNA. Translation: AAB04945.1.
L00630 Genomic DNA. Translation: AAA35148.1.
Y07703 Genomic DNA. Translation: CAA68968.1.
Z73031 Genomic DNA. Translation: CAA97275.1.
BK006941 Genomic DNA. Translation: DAA08337.1.
PIRA44072.
RefSeqNP_011762.1. NM_001181375.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95B/F/J/N437-506[»]
ProteinModelPortalP29056.
SMRP29056. Positions 74-262, 437-506.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33497. 34 interactions.
DIPDIP-191N.
IntActP29056. 13 interactions.
MINTMINT-2783113.
STRING4932.YGR246C.

Proteomic databases

MaxQBP29056.
PaxDbP29056.
PeptideAtlasP29056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR246C; YGR246C; YGR246C.
GeneID853161.
KEGGsce:YGR246C.

Organism-specific databases

CYGDYGR246c.
SGDS000003478. BRF1.

Phylogenomic databases

eggNOGCOG1405.
GeneTreeENSGT00390000010349.
HOGENOMHOG000198688.
KOK15196.
OMAIAGACIL.
OrthoDBEOG7Q5HPM.

Enzyme and pathway databases

BioCycYEAST:G3O-30921-MONOMER.

Gene expression databases

GenevestigatorP29056.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
InterProIPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERPTHR11618. PTHR11618. 1 hit.
PfamPF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSPR00685. TIFACTORIIB.
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
PROSITEPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP29056.
NextBio973264.
PROP29056.

Entry information

Entry nameTF3B_YEAST
AccessionPrimary (citable) accession number: P29056
Secondary accession number(s): D6VV26
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references