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P29056

- TF3B_YEAST

UniProt

P29056 - TF3B_YEAST

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Protein

Transcription factor IIIB 70 kDa subunit

Gene

BRF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi4 – 41ZincBy similarity
Metal bindingi7 – 71ZincBy similarity
Metal bindingi25 – 251ZincBy similarity
Metal bindingi28 – 281ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 3333TFIIB-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: SGD
  2. RNA polymerase III core binding Source: SGD
  3. TFIIIB-type transcription factor activity Source: SGD
  4. TFIIIC-class transcription factor binding Source: SGD
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA-templated transcriptional open complex formation Source: SGD
  2. negative regulation of protein homodimerization activity Source: SGD
  3. regulation of transcription from RNA polymerase III promoter Source: GOC
  4. RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  5. transposon integration Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30921-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 70 kDa subunit
Short name:
TFIIIB
Alternative name(s):
B-related factor 1
Short name:
BRF-1
Gene namesi
Name:BRF1
Synonyms:PCF4, TDS4
Ordered Locus Names:YGR246C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR246c.
SGDiS000003478. BRF1.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
  2. transcription factor TFIIIB complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 596596Transcription factor IIIB 70 kDa subunitPRO_0000119346Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei381 – 3811Phosphoserine2 Publications
Modified residuei384 – 3841Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP29056.
PaxDbiP29056.
PeptideAtlasiP29056.

Expressioni

Gene expression databases

GenevestigatoriP29056.

Interactioni

Subunit structurei

TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and the B" component (TFC5).

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT15P133932EBI-19142,EBI-19129

Protein-protein interaction databases

BioGridi33497. 34 interactions.
DIPiDIP-191N.
IntActiP29056. 13 interactions.
MINTiMINT-2783113.
STRINGi4932.YGR246C.

Structurei

Secondary structure

1
596
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi442 – 4443
Helixi448 – 4525
Helixi470 – 4723
Helixi477 – 49014
Turni491 – 4933
Helixi494 – 5007
Turni501 – 5055

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NGMX-ray2.95B/F/J/N437-506[»]
ProteinModelPortaliP29056.
SMRiP29056. Positions 74-262, 437-506.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29056.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati90 – 166771Add
BLAST
Repeati185 – 264802Add
BLAST

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 3333TFIIB-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG1405.
GeneTreeiENSGT00390000010349.
HOGENOMiHOG000198688.
InParanoidiP29056.
KOiK15196.
OMAiIAGACIL.
OrthoDBiEOG7Q5HPM.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR029529. Brf1.
IPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PTHR11618:SF4. PTHR11618:SF4. 1 hit.
PfamiPF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29056-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA
60 70 80 90 100
AVVQGSFIGA GQSHAAFGGS SALESREATL NNARRKLRAV SYALHIPEYI
110 120 130 140 150
TDAAFQWYKL ALANNFVQGR RSQNVIASCL YVACRKEKTH HMLIDFSSRL
160 170 180 190 200
QVSVYSIGAT FLKMVKKLHI TELPLADPSL FIQHFAEKLD LADKKIKVVK
210 220 230 240 250
DAVKLAQRMS KDWMFEGRRP AGIAGACILL ACRMNNLRRT HTEIVAVSHV
260 270 280 290 300
AEETLQQRLN EFKNTKAAKL SVQKFRENDV EDGEARPPSF VKNRKKERKI
310 320 330 340 350
KDSLDKEEMF QTSEEALNKN PILTQVLGEQ ELSSKEVLFY LKQFSERRAR
360 370 380 390 400
VVERIKATNG IDGENIYHEG SENETRKRKL SEVSIQNEHV EGEDKETEGT
410 420 430 440 450
EEKVKKVKTK TSEEKKENES GHFQDAIDGY SLETDPYCPR NLHLLPTTDT
460 470 480 490 500
YLSKVSDDPD NLEDVDDEEL NAHLLNEEAS KLKERIWIGL NADFLLEQES
510 520 530 540 550
KRLKQEADIA TGNTSVKKKR TRRRNNTRSD EPTKTVDAAA AIGLMSDLQD
560 570 580 590
KSGLHAALKA AEESGDFTTA DSVKNMLQKA SFSKKINYDA IDGLFR
Length:596
Mass (Da):66,906
Last modified:December 1, 1992 - v1
Checksum:i489207643A66B796
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91073 Genomic DNA. Translation: AAB04945.1.
L00630 Genomic DNA. Translation: AAA35148.1.
Y07703 Genomic DNA. Translation: CAA68968.1.
Z73031 Genomic DNA. Translation: CAA97275.1.
BK006941 Genomic DNA. Translation: DAA08337.1.
PIRiA44072.
RefSeqiNP_011762.1. NM_001181375.1.

Genome annotation databases

EnsemblFungiiYGR246C; YGR246C; YGR246C.
GeneIDi853161.
KEGGisce:YGR246C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91073 Genomic DNA. Translation: AAB04945.1 .
L00630 Genomic DNA. Translation: AAA35148.1 .
Y07703 Genomic DNA. Translation: CAA68968.1 .
Z73031 Genomic DNA. Translation: CAA97275.1 .
BK006941 Genomic DNA. Translation: DAA08337.1 .
PIRi A44072.
RefSeqi NP_011762.1. NM_001181375.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NGM X-ray 2.95 B/F/J/N 437-506 [» ]
ProteinModelPortali P29056.
SMRi P29056. Positions 74-262, 437-506.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33497. 34 interactions.
DIPi DIP-191N.
IntActi P29056. 13 interactions.
MINTi MINT-2783113.
STRINGi 4932.YGR246C.

Proteomic databases

MaxQBi P29056.
PaxDbi P29056.
PeptideAtlasi P29056.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR246C ; YGR246C ; YGR246C .
GeneIDi 853161.
KEGGi sce:YGR246C.

Organism-specific databases

CYGDi YGR246c.
SGDi S000003478. BRF1.

Phylogenomic databases

eggNOGi COG1405.
GeneTreei ENSGT00390000010349.
HOGENOMi HOG000198688.
InParanoidi P29056.
KOi K15196.
OMAi IAGACIL.
OrthoDBi EOG7Q5HPM.

Enzyme and pathway databases

BioCyci YEAST:G3O-30921-MONOMER.

Miscellaneous databases

EvolutionaryTracei P29056.
NextBioi 973264.
PROi P29056.

Gene expression databases

Genevestigatori P29056.

Family and domain databases

Gene3Di 1.10.472.10. 2 hits.
InterProi IPR029529. Brf1.
IPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view ]
PANTHERi PTHR11618. PTHR11618. 1 hit.
PTHR11618:SF4. PTHR11618:SF4. 1 hit.
Pfami PF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view ]
PRINTSi PR00685. TIFACTORIIB.
SMARTi SM00385. CYCLIN. 2 hits.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 2 hits.
PROSITEi PS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PCF4 encodes an RNA polymerase III transcription factor with homology to TFIIB."
    Lopez-De-Leon A., Librizzi M., Puglia K., Willis I.M.
    Cell 71:211-220(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB."
    Buratowski S., Zhou H.
    Cell 71:221-230(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A yeast TFIIB-related factor involved in RNA polymerase III transcription."
    Colbert T., Hahn S.
    Genes Dev. 6:1940-1949(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII reveals the presence of eight open reading frames, including BRF1 (TFIIIB70) and GCN5 genes."
    Feroli F., Carignani G., Pavanello A., Guerreiro P., Azevedo D., Rodrigues-Pousada C., Melchioretto P., Panzeri L., Agostoni Carbone M.L.
    Yeast 13:373-377(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381 AND SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTF3B_YEAST
AccessioniPrimary (citable) accession number: P29056
Secondary accession number(s): D6VV26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3