Transcription factor IIIB 70 kDa subunit - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P29056 (TF3B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 130. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcription factor IIIB 70 kDa subunit
Short name=TFIIIB

Alternative name(s):
B-related factor 1
Short name=BRF-1

Gene names

Name:	BRF1
Synonyms:	PCF4, TDS4
Ordered Locus Names:	YGR246C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and the B" component (TFC5).
Subcellular location	Nucleus.
Miscellaneous	Present with 4330 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcriptional open complex formation Inferred from mutant phenotype PubMed 11387215. Source: SGD RNA polymerase III transcriptional preinitiation complex assembly Inferred from mutant phenotype PubMed 11387215 PubMed 9218475 PubMed 9710642. Source: SGD negative regulation of protein homodimerization activity Inferred from direct assay PubMed 15190063. Source: SGD positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transposon integration Inferred from direct assay PubMed 10882723. Source: SGD
Cellular_component	transcription factor TFIIIB complex Inferred from direct assay Ref.2 PubMed 1458536. Source: SGD
Molecular_function	DNA binding Inferred from direct assay PubMed 9271407. Source: SGD RNA polymerase III core binding Inferred from direct assay PubMed 7995525 PubMed 9689057. Source: SGD TFIIIB-type transcription factor activity Inferred by curator PubMed 11387215 PubMed 12930823 PubMed 1458536 PubMed 7995525. Source: SGD TFIIIC-class transcription factor binding Inferred from direct assay PubMed 12930823. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPT15	P13393	2	EBI-19142,EBI-19129

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 596

596

Transcription factor IIIB 70 kDa subunit

PRO_0000119346

Regions

Repeat

90 – 166

Repeat

185 – 264

Zinc finger

1 – 33

TFIIB-type

Sites

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Amino acid modifications

Modified residue

381

Phosphoserine Ref.8

Modified residue

384

Phosphoserine Ref.8 Ref.9

Secondary structure

596

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P29056 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 489207643A66B796
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FASTA

596

66,906

        10         20         30         40         50         60 
MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA AVVQGSFIGA 

        70         80         90        100        110        120 
GQSHAAFGGS SALESREATL NNARRKLRAV SYALHIPEYI TDAAFQWYKL ALANNFVQGR 

       130        140        150        160        170        180 
RSQNVIASCL YVACRKEKTH HMLIDFSSRL QVSVYSIGAT FLKMVKKLHI TELPLADPSL 

       190        200        210        220        230        240 
FIQHFAEKLD LADKKIKVVK DAVKLAQRMS KDWMFEGRRP AGIAGACILL ACRMNNLRRT 

       250        260        270        280        290        300 
HTEIVAVSHV AEETLQQRLN EFKNTKAAKL SVQKFRENDV EDGEARPPSF VKNRKKERKI 

       310        320        330        340        350        360 
KDSLDKEEMF QTSEEALNKN PILTQVLGEQ ELSSKEVLFY LKQFSERRAR VVERIKATNG 

       370        380        390        400        410        420 
IDGENIYHEG SENETRKRKL SEVSIQNEHV EGEDKETEGT EEKVKKVKTK TSEEKKENES 

       430        440        450        460        470        480 
GHFQDAIDGY SLETDPYCPR NLHLLPTTDT YLSKVSDDPD NLEDVDDEEL NAHLLNEEAS 

       490        500        510        520        530        540 
KLKERIWIGL NADFLLEQES KRLKQEADIA TGNTSVKKKR TRRRNNTRSD EPTKTVDAAA 

       550        560        570        580        590 
AIGLMSDLQD KSGLHAALKA AEESGDFTTA DSVKNMLQKA SFSKKINYDA IDGLFR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PCF4 encodes an RNA polymerase III transcription factor with homology to TFIIB." Lopez-De-Leon A., Librizzi M., Puglia K., Willis I.M. Cell 71:211-220(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB." Buratowski S., Zhou H. Cell 71:221-230(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A yeast TFIIB-related factor involved in RNA polymerase III transcription." Colbert T., Hahn S. Genes Dev. 6:1940-1949(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII reveals the presence of eight open reading frames, including BRF1 (TFIIIB70) and GCN5 genes." Feroli F., Carignani G., Pavanello A., Guerreiro P., Azevedo D., Rodrigues-Pousada C., Melchioretto P., Panzeri L., Agostoni Carbone M.L. Yeast 13:373-377(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381 AND SER-384, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M91073 Genomic DNA. Translation: AAB04945.1.
L00630 Genomic DNA. Translation: AAA35148.1.
Y07703 Genomic DNA. Translation: CAA68968.1.
Z73031 Genomic DNA. Translation: CAA97275.1.
BK006941 Genomic DNA. Translation: DAA08337.1.

PIR

A44072.

RefSeq

NP_011762.1. NM_001181375.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NGM	X-ray	2.95	B/F/J/N	437-506	[»]

ProteinModelPortal

P29056.

SMR

P29056. Positions 74-262, 437-506.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-191N.

IntAct

P29056. 13 interactions.

MINT

MINT-2783113.

STRING

4932.YGR246C.

Proteomic databases

PaxDb

P29056.

PeptideAtlas

P29056.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR246C; YGR246C; YGR246C.

GeneID

853161.

KEGG

sce:YGR246C.

Organism-specific databases

CYGD

YGR246c.

SGD

S000003478. BRF1.

Phylogenomic databases

eggNOG

COG1405.

GeneTree

ENSGT00390000010349.

HOGENOM

HOG000198688.

K15196.

OMA

CRKERTH.

OrthoDB

EOG40P7GP.

Enzyme and pathway databases

Reactome

REACT_83470. Gene Expression.
REACT_87991. Transcription.

Gene expression databases

Genevestigator

P29056.

GermOnline

YGR246C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.472.10. 2 hits.

InterPro

IPR011665. BRF1_TBP-bd.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]

PANTHER

PTHR11618. PTHR11618. 1 hit.

Pfam

PF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]

PRINTS

PR00685. TIFACTORIIB.

SMART

SM00385. CYCLIN. 2 hits.
[Graphical view]

SUPFAM

SSF47954. Cyclin_like. 2 hits.

PROSITE

PS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P29056.

NextBio

973264.

Entry information

Entry name

TF3B_YEAST

Accession

Primary (citable) accession number: P29056
Secondary accession number(s): D6VV26

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 130 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents