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P29056

- TF3B_YEAST

UniProt

P29056 - TF3B_YEAST

Protein

Transcription factor IIIB 70 kDa subunit

Gene

BRF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi4 – 41ZincBy similarity
    Metal bindingi7 – 71ZincBy similarity
    Metal bindingi25 – 251ZincBy similarity
    Metal bindingi28 – 281ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1 – 3333TFIIB-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: SGD
    2. protein binding Source: IntAct
    3. RNA polymerase III core binding Source: SGD
    4. TFIIIB-type transcription factor activity Source: SGD
    5. TFIIIC-class transcription factor binding Source: SGD
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA-templated transcriptional open complex formation Source: SGD
    2. negative regulation of protein homodimerization activity Source: SGD
    3. positive regulation of transcription, DNA-templated Source: InterPro
    4. regulation of transcription from RNA polymerase III promoter Source: GOC
    5. RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
    6. transposon integration Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30921-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor IIIB 70 kDa subunit
    Short name:
    TFIIIB
    Alternative name(s):
    B-related factor 1
    Short name:
    BRF-1
    Gene namesi
    Name:BRF1
    Synonyms:PCF4, TDS4
    Ordered Locus Names:YGR246C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR246c.
    SGDiS000003478. BRF1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome
    2. transcription factor TFIIIB complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 596596Transcription factor IIIB 70 kDa subunitPRO_0000119346Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei381 – 3811Phosphoserine2 Publications
    Modified residuei384 – 3841Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP29056.
    PaxDbiP29056.
    PeptideAtlasiP29056.

    Expressioni

    Gene expression databases

    GenevestigatoriP29056.

    Interactioni

    Subunit structurei

    TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and the B" component (TFC5).

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SPT15P133932EBI-19142,EBI-19129

    Protein-protein interaction databases

    BioGridi33497. 34 interactions.
    DIPiDIP-191N.
    IntActiP29056. 13 interactions.
    MINTiMINT-2783113.
    STRINGi4932.YGR246C.

    Structurei

    Secondary structure

    1
    596
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi442 – 4443
    Helixi448 – 4525
    Helixi470 – 4723
    Helixi477 – 49014
    Turni491 – 4933
    Helixi494 – 5007
    Turni501 – 5055

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NGMX-ray2.95B/F/J/N437-506[»]
    ProteinModelPortaliP29056.
    SMRiP29056. Positions 74-262, 437-506.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP29056.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati90 – 166771Add
    BLAST
    Repeati185 – 264802Add
    BLAST

    Sequence similaritiesi

    Belongs to the TFIIB family.Curated
    Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1 – 3333TFIIB-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1405.
    GeneTreeiENSGT00390000010349.
    HOGENOMiHOG000198688.
    KOiK15196.
    OMAiIAGACIL.
    OrthoDBiEOG7Q5HPM.

    Family and domain databases

    Gene3Di1.10.472.10. 2 hits.
    InterProiIPR029529. Brf1.
    IPR011665. BRF1_TBP-bd_dom.
    IPR013763. Cyclin-like.
    IPR000812. TFIIB.
    IPR023486. TFIIB_CS.
    IPR013150. TFIIB_cyclin.
    IPR013137. Znf_TFIIB.
    [Graphical view]
    PANTHERiPTHR11618. PTHR11618. 1 hit.
    PTHR11618:SF4. PTHR11618:SF4. 1 hit.
    PfamiPF07741. BRF1. 1 hit.
    PF08271. TF_Zn_Ribbon. 1 hit.
    PF00382. TFIIB. 2 hits.
    [Graphical view]
    PRINTSiPR00685. TIFACTORIIB.
    SMARTiSM00385. CYCLIN. 2 hits.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 2 hits.
    PROSITEiPS00782. TFIIB. 2 hits.
    PS51134. ZF_TFIIB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29056-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA    50
    AVVQGSFIGA GQSHAAFGGS SALESREATL NNARRKLRAV SYALHIPEYI 100
    TDAAFQWYKL ALANNFVQGR RSQNVIASCL YVACRKEKTH HMLIDFSSRL 150
    QVSVYSIGAT FLKMVKKLHI TELPLADPSL FIQHFAEKLD LADKKIKVVK 200
    DAVKLAQRMS KDWMFEGRRP AGIAGACILL ACRMNNLRRT HTEIVAVSHV 250
    AEETLQQRLN EFKNTKAAKL SVQKFRENDV EDGEARPPSF VKNRKKERKI 300
    KDSLDKEEMF QTSEEALNKN PILTQVLGEQ ELSSKEVLFY LKQFSERRAR 350
    VVERIKATNG IDGENIYHEG SENETRKRKL SEVSIQNEHV EGEDKETEGT 400
    EEKVKKVKTK TSEEKKENES GHFQDAIDGY SLETDPYCPR NLHLLPTTDT 450
    YLSKVSDDPD NLEDVDDEEL NAHLLNEEAS KLKERIWIGL NADFLLEQES 500
    KRLKQEADIA TGNTSVKKKR TRRRNNTRSD EPTKTVDAAA AIGLMSDLQD 550
    KSGLHAALKA AEESGDFTTA DSVKNMLQKA SFSKKINYDA IDGLFR 596
    Length:596
    Mass (Da):66,906
    Last modified:December 1, 1992 - v1
    Checksum:i489207643A66B796
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91073 Genomic DNA. Translation: AAB04945.1.
    L00630 Genomic DNA. Translation: AAA35148.1.
    Y07703 Genomic DNA. Translation: CAA68968.1.
    Z73031 Genomic DNA. Translation: CAA97275.1.
    BK006941 Genomic DNA. Translation: DAA08337.1.
    PIRiA44072.
    RefSeqiNP_011762.1. NM_001181375.1.

    Genome annotation databases

    EnsemblFungiiYGR246C; YGR246C; YGR246C.
    GeneIDi853161.
    KEGGisce:YGR246C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91073 Genomic DNA. Translation: AAB04945.1 .
    L00630 Genomic DNA. Translation: AAA35148.1 .
    Y07703 Genomic DNA. Translation: CAA68968.1 .
    Z73031 Genomic DNA. Translation: CAA97275.1 .
    BK006941 Genomic DNA. Translation: DAA08337.1 .
    PIRi A44072.
    RefSeqi NP_011762.1. NM_001181375.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NGM X-ray 2.95 B/F/J/N 437-506 [» ]
    ProteinModelPortali P29056.
    SMRi P29056. Positions 74-262, 437-506.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33497. 34 interactions.
    DIPi DIP-191N.
    IntActi P29056. 13 interactions.
    MINTi MINT-2783113.
    STRINGi 4932.YGR246C.

    Proteomic databases

    MaxQBi P29056.
    PaxDbi P29056.
    PeptideAtlasi P29056.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR246C ; YGR246C ; YGR246C .
    GeneIDi 853161.
    KEGGi sce:YGR246C.

    Organism-specific databases

    CYGDi YGR246c.
    SGDi S000003478. BRF1.

    Phylogenomic databases

    eggNOGi COG1405.
    GeneTreei ENSGT00390000010349.
    HOGENOMi HOG000198688.
    KOi K15196.
    OMAi IAGACIL.
    OrthoDBi EOG7Q5HPM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30921-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P29056.
    NextBioi 973264.
    PROi P29056.

    Gene expression databases

    Genevestigatori P29056.

    Family and domain databases

    Gene3Di 1.10.472.10. 2 hits.
    InterProi IPR029529. Brf1.
    IPR011665. BRF1_TBP-bd_dom.
    IPR013763. Cyclin-like.
    IPR000812. TFIIB.
    IPR023486. TFIIB_CS.
    IPR013150. TFIIB_cyclin.
    IPR013137. Znf_TFIIB.
    [Graphical view ]
    PANTHERi PTHR11618. PTHR11618. 1 hit.
    PTHR11618:SF4. PTHR11618:SF4. 1 hit.
    Pfami PF07741. BRF1. 1 hit.
    PF08271. TF_Zn_Ribbon. 1 hit.
    PF00382. TFIIB. 2 hits.
    [Graphical view ]
    PRINTSi PR00685. TIFACTORIIB.
    SMARTi SM00385. CYCLIN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 2 hits.
    PROSITEi PS00782. TFIIB. 2 hits.
    PS51134. ZF_TFIIB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PCF4 encodes an RNA polymerase III transcription factor with homology to TFIIB."
      Lopez-De-Leon A., Librizzi M., Puglia K., Willis I.M.
      Cell 71:211-220(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB."
      Buratowski S., Zhou H.
      Cell 71:221-230(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "A yeast TFIIB-related factor involved in RNA polymerase III transcription."
      Colbert T., Hahn S.
      Genes Dev. 6:1940-1949(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII reveals the presence of eight open reading frames, including BRF1 (TFIIIB70) and GCN5 genes."
      Feroli F., Carignani G., Pavanello A., Guerreiro P., Azevedo D., Rodrigues-Pousada C., Melchioretto P., Panzeri L., Agostoni Carbone M.L.
      Yeast 13:373-377(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381 AND SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTF3B_YEAST
    AccessioniPrimary (citable) accession number: P29056
    Secondary accession number(s): D6VV26
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4330 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3