Reviewed, UniProtKB/Swiss-Prot P29055 (TF2B_YEAST)

Names and origin

Protein names

Recommended name:
    Transcription initiation factor IIB
Alternative name(s):
    General transcription factor TFIIB
    Transcription factor E

Gene names

Name:	SUA7
Ordered Locus Names:	YPR086W
ORF Names:	P9513.4

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.
Subcellular location	Nucleus.
Miscellaneous	Present with 6750 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription initiation from RNA polymerase II promoter Inferred from direct assay. Source: SGD translational initiation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleoplasm Inferred from physical interaction. Source: SGD
Molecular function	general RNA polymerase II transcription factor activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ECM4	P36156	1	EBI-19123,EBI-2042717
MRPL24	P36525	1	EBI-19123,EBI-15545
RPB5	P20434	1	EBI-19123,EBI-15781
RPP1A	P05318	1	EBI-19123,EBI-15452

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Transcription initiation factor IIB		PRO_0000119308
Regions
	Repeat	136 – 212	77	1
	Repeat	242 – 318	77	2
	Zinc finger	20 – 53	34	TFIIB-type
Sites
	Metal binding	24	1	Zinc By similarity
	Metal binding	27	1	Zinc By similarity
	Metal binding	45	1	Zinc By similarity
	Metal binding	48	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29055-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 8F1F6D24602436E2	FASTA	345	38,200
	10 20 30 40 50 60 MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR 70 80 90 100 110 120 SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE TTDMRFTKEL NKAQGKNVMD 130 140 150 160 170 180 KKDNEVQAAF AKITMLCDAA ELPKIVKDCA KEAYKLCHDE KTLKGKSMES IMAASILIGC 190 200 210 220 230 240 RRAEVARTFK EIQSLIHVKT KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP 250 260 270 280 290 300 RFCSHLGLPM QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ 310 320 330 340 TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo." Pinto I., Ware D.E., Hampsey M. Cell 68:977-988(1992) [PubMed: 1547497] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M81380 Genomic DNA. Translation: AAA35126.1.
U51033 Genomic DNA. Translation: AAB68135.1.

PIR

S26707.

RefSeq

NP_015411.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3K1F	X-ray	4.30	M	1-68	[»]
3K7A	X-ray	3.80	M	1-345	[»]

SMR

P29055. Positions 17-70, 123-341.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-701N.

IntAct

P29055. 157 interactions.

STRING

P29055.

Proteomic databases

PeptideAtlas

P29055.

Genome annotation databases

Ensembl

YPR086W; YPR086W; YPR086W; Saccharomyces cerevisiae. [Genome view]

GeneID

856201.

KEGG

sce:YPR086W.

NMPDR

fig|4932.3.peg.6548.

Organism-specific databases

CYGD

YPR086w.

SGD

S000006290. SUA7.

Phylogenomic databases

eggNOG

fuNOG05097.

HOGENOM

HBG646536.

OMA

RTFSNEK.

OrthoDB

EOG9PP00R.

PhylomeDB

P29055.

Gene expression databases

ArrayExpress

P29055.

Genevestigator

P29055.

GermOnline

YPR086W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR006670. Cyclin.
IPR011028. Cyclin-like.
IPR013763. Cyclin_related.
IPR000812. TFIIB-related.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]

Gene3D

G3DSA:1.10.472.10. Cyclin_related. 2 hits.

PANTHER

PTHR11618. TFIIB_euk_relate. 1 hit.

Pfam

PF00382. TFIIB. 2 hits.
PF08271. TFIIB_Zn_Ribbon. 1 hit.
[Graphical view]

PRINTS

PR00685. TIFACTORIIB.

SMART

SM00385. CYCLIN. 2 hits.
[Graphical view]

PROSITE

PS00782. TFIIB. 1 hit.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

981402.

Entry information

Entry name

TF2B_YEAST

Accession

Primary (citable) accession number: P29055

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 19, 2010
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)