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P29055

- TF2B_YEAST

UniProt

P29055 - TF2B_YEAST

Protein

Transcription initiation factor IIB

Gene

SUA7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi24 – 241ZincBy similarity
    Metal bindingi27 – 271ZincBy similarity
    Metal bindingi45 – 451ZincBy similarity
    Metal bindingi48 – 481ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri20 – 5334TFIIB-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core RNA polymerase II recruiting transcription factor activity Source: SGD
    2. RNA polymerase II core binding Source: SGD
    3. TBP-class protein binding Source: SGD
    4. TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly Source: SGD
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. positive regulation of transcription regulatory region DNA binding Source: SGD
    2. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    3. transcriptional open complex formation at RNA polymerase II promoter Source: SGD
    4. transcriptional start site selection at RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34230-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor IIB
    Alternative name(s):
    General transcription factor TFIIB
    Transcription factor E
    Gene namesi
    Name:SUA7
    Ordered Locus Names:YPR086W
    ORF Names:P9513.4
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR086w.
    SGDiS000006290. SUA7.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 345345Transcription initiation factor IIBPRO_0000119308Add
    BLAST

    Proteomic databases

    MaxQBiP29055.
    PaxDbiP29055.
    PeptideAtlasiP29055.

    Expressioni

    Gene expression databases

    GenevestigatoriP29055.

    Interactioni

    Subunit structurei

    Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.

    Protein-protein interaction databases

    BioGridi36257. 75 interactions.
    DIPiDIP-701N.
    IntActiP29055. 56 interactions.
    MINTiMINT-473341.
    STRINGi4932.YPR086W.

    Structurei

    Secondary structure

    1
    345
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 374
    Turni38 – 414
    Beta strandi42 – 454
    Turni46 – 483
    Beta strandi50 – 545
    Helixi59 – 635
    Turni64 – 663
    Beta strandi71 – 733
    Beta strandi80 – 834
    Helixi85 – 884
    Beta strandi95 – 973
    Helixi104 – 11613
    Beta strandi122 – 1254
    Helixi128 – 13912
    Helixi144 – 15714
    Turni161 – 1655
    Helixi168 – 18215
    Helixi189 – 1968
    Helixi202 – 21211

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3K1FX-ray4.30M1-68[»]
    3K7AX-ray3.80M1-345[»]
    4BBRX-ray3.40M1-345[»]
    4BBSX-ray3.60M1-345[»]
    ProteinModelPortaliP29055.
    SMRiP29055. Positions 22-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP29055.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati136 – 212771Add
    BLAST
    Repeati242 – 318772Add
    BLAST

    Sequence similaritiesi

    Belongs to the TFIIB family.Curated
    Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri20 – 5334TFIIB-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1405.
    GeneTreeiENSGT00390000006671.
    HOGENOMiHOG000108634.
    KOiK03124.
    OMAiEYTERES.
    OrthoDBiEOG7RV9S3.

    Family and domain databases

    Gene3Di1.10.472.10. 2 hits.
    InterProiIPR013763. Cyclin-like.
    IPR000812. TFIIB.
    IPR023486. TFIIB_CS.
    IPR013150. TFIIB_cyclin.
    IPR013137. Znf_TFIIB.
    [Graphical view]
    PANTHERiPTHR11618. PTHR11618. 1 hit.
    PfamiPF08271. TF_Zn_Ribbon. 1 hit.
    PF00382. TFIIB. 2 hits.
    [Graphical view]
    PRINTSiPR00685. TIFACTORIIB.
    SMARTiSM00385. CYCLIN. 2 hits.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 2 hits.
    PROSITEiPS00782. TFIIB. 1 hit.
    PS51134. ZF_TFIIB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29055-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL    50
    VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE 100
    TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA 150
    KEAYKLCHDE KTLKGKSMES IMAASILIGC RRAEVARTFK EIQSLIHVKT 200
    KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP RFCSHLGLPM 250
    QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ 300
    TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK 345
    Length:345
    Mass (Da):38,200
    Last modified:December 1, 1992 - v1
    Checksum:i8F1F6D24602436E2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101R → L in AAT93251. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M81380 Genomic DNA. Translation: AAA35126.1.
    U51033 Genomic DNA. Translation: AAB68135.1.
    AY693232 Genomic DNA. Translation: AAT93251.1.
    BK006949 Genomic DNA. Translation: DAA11504.1.
    PIRiS26707.
    RefSeqiNP_015411.1. NM_001184183.1.

    Genome annotation databases

    EnsemblFungiiYPR086W; YPR086W; YPR086W.
    GeneIDi856201.
    KEGGisce:YPR086W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M81380 Genomic DNA. Translation: AAA35126.1 .
    U51033 Genomic DNA. Translation: AAB68135.1 .
    AY693232 Genomic DNA. Translation: AAT93251.1 .
    BK006949 Genomic DNA. Translation: DAA11504.1 .
    PIRi S26707.
    RefSeqi NP_015411.1. NM_001184183.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3K1F X-ray 4.30 M 1-68 [» ]
    3K7A X-ray 3.80 M 1-345 [» ]
    4BBR X-ray 3.40 M 1-345 [» ]
    4BBS X-ray 3.60 M 1-345 [» ]
    ProteinModelPortali P29055.
    SMRi P29055. Positions 22-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36257. 75 interactions.
    DIPi DIP-701N.
    IntActi P29055. 56 interactions.
    MINTi MINT-473341.
    STRINGi 4932.YPR086W.

    Proteomic databases

    MaxQBi P29055.
    PaxDbi P29055.
    PeptideAtlasi P29055.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR086W ; YPR086W ; YPR086W .
    GeneIDi 856201.
    KEGGi sce:YPR086W.

    Organism-specific databases

    CYGDi YPR086w.
    SGDi S000006290. SUA7.

    Phylogenomic databases

    eggNOGi COG1405.
    GeneTreei ENSGT00390000006671.
    HOGENOMi HOG000108634.
    KOi K03124.
    OMAi EYTERES.
    OrthoDBi EOG7RV9S3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34230-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P29055.
    NextBioi 981402.
    PROi P29055.

    Gene expression databases

    Genevestigatori P29055.

    Family and domain databases

    Gene3Di 1.10.472.10. 2 hits.
    InterProi IPR013763. Cyclin-like.
    IPR000812. TFIIB.
    IPR023486. TFIIB_CS.
    IPR013150. TFIIB_cyclin.
    IPR013137. Znf_TFIIB.
    [Graphical view ]
    PANTHERi PTHR11618. PTHR11618. 1 hit.
    Pfami PF08271. TF_Zn_Ribbon. 1 hit.
    PF00382. TFIIB. 2 hits.
    [Graphical view ]
    PRINTSi PR00685. TIFACTORIIB.
    SMARTi SM00385. CYCLIN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 2 hits.
    PROSITEi PS00782. TFIIB. 1 hit.
    PS51134. ZF_TFIIB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo."
      Pinto I., Ware D.E., Hampsey M.
      Cell 68:977-988(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTF2B_YEAST
    AccessioniPrimary (citable) accession number: P29055
    Secondary accession number(s): D6W488, E9P939
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6750 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3