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P29055 (TF2B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor IIB
Alternative name(s):
General transcription factor TFIIB
Transcription factor E
Gene names
Name:SUA7
Ordered Locus Names:YPR086W
ORF Names:P9513.4
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.

Subcellular location

Nucleus.

Miscellaneous

Present with 6750 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TFIIB family.

Contains 1 TFIIB-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Transcription initiation factor IIB
PRO_0000119308

Regions

Repeat136 – 212771
Repeat242 – 318772
Zinc finger20 – 5334TFIIB-type

Sites

Metal binding241Zinc By similarity
Metal binding271Zinc By similarity
Metal binding451Zinc By similarity
Metal binding481Zinc By similarity

Experimental info

Sequence conflict101R → L in AAT93251. Ref.4

Secondary structure

................................... 345
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29055 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 8F1F6D24602436E2

FASTA34538,200
        10         20         30         40         50         60 
MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR 

        70         80         90        100        110        120 
SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE TTDMRFTKEL NKAQGKNVMD 

       130        140        150        160        170        180 
KKDNEVQAAF AKITMLCDAA ELPKIVKDCA KEAYKLCHDE KTLKGKSMES IMAASILIGC 

       190        200        210        220        230        240 
RRAEVARTFK EIQSLIHVKT KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP 

       250        260        270        280        290        300 
RFCSHLGLPM QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ 

       310        320        330        340 
TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK 

« Hide

References

« Hide 'large scale' references
[1]"The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo."
Pinto I., Ware D.E., Hampsey M.
Cell 68:977-988(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M81380 Genomic DNA. Translation: AAA35126.1.
U51033 Genomic DNA. Translation: AAB68135.1.
AY693232 Genomic DNA. Translation: AAT93251.1.
BK006949 Genomic DNA. Translation: DAA11504.1.
PIRS26707.
RefSeqNP_015411.1. NM_001184183.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3K1FX-ray4.30M1-68[»]
3K7AX-ray3.80M1-345[»]
4BBRX-ray3.40M1-345[»]
4BBSX-ray3.60M1-345[»]
ProteinModelPortalP29055.
SMRP29055. Positions 22-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36257. 73 interactions.
DIPDIP-701N.
IntActP29055. 56 interactions.
MINTMINT-473341.
STRING4932.YPR086W.

Proteomic databases

MaxQBP29055.
PaxDbP29055.
PeptideAtlasP29055.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR086W; YPR086W; YPR086W.
GeneID856201.
KEGGsce:YPR086W.

Organism-specific databases

CYGDYPR086w.
SGDS000006290. SUA7.

Phylogenomic databases

eggNOGCOG1405.
GeneTreeENSGT00390000006671.
HOGENOMHOG000108634.
KOK03124.
OMAEYTERES.
OrthoDBEOG7RV9S3.

Enzyme and pathway databases

BioCycYEAST:G3O-34230-MONOMER.

Gene expression databases

GenevestigatorP29055.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
InterProIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERPTHR11618. PTHR11618. 1 hit.
PfamPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSPR00685. TIFACTORIIB.
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
PROSITEPS00782. TFIIB. 1 hit.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP29055.
NextBio981402.
PROP29055.

Entry information

Entry nameTF2B_YEAST
AccessionPrimary (citable) accession number: P29055
Secondary accession number(s): D6W488, E9P939
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 14, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references