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Protein

Transcription initiation factor IIB

Gene

SUA7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24ZincBy similarity1
Metal bindingi27ZincBy similarity1
Metal bindingi45ZincBy similarity1
Metal bindingi48ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri20 – 53TFIIB-typePROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

  • RNA polymerase II core binding Source: SGD
  • RNA polymerase II transcription factor activity, TBP-class protein binding, involved in preinitiation complex assembly Source: SGD
  • TBP-class protein binding Source: SGD
  • transcription factor activity, core RNA polymerase II recruiting Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • positive regulation of transcription regulatory region DNA binding Source: SGD
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • transcriptional open complex formation at RNA polymerase II promoter Source: SGD
  • transcriptional start site selection at RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34230-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIB
Alternative name(s):
General transcription factor TFIIB
Transcription factor E
Gene namesi
Name:SUA7
Ordered Locus Names:YPR086W
ORF Names:P9513.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR086W.
SGDiS000006290. SUA7.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • transcriptional preinitiation complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001193081 – 345Transcription initiation factor IIBAdd BLAST345

Proteomic databases

MaxQBiP29055.
PRIDEiP29055.

Interactioni

Subunit structurei

Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.

GO - Molecular functioni

  • RNA polymerase II core binding Source: SGD
  • TBP-class protein binding Source: SGD

Protein-protein interaction databases

BioGridi36257. 76 interactors.
DIPiDIP-701N.
IntActiP29055. 57 interactors.
MINTiMINT-473341.

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 37Combined sources4
Turni38 – 41Combined sources4
Beta strandi42 – 45Combined sources4
Turni46 – 48Combined sources3
Beta strandi50 – 54Combined sources5
Helixi59 – 63Combined sources5
Turni64 – 66Combined sources3
Beta strandi71 – 73Combined sources3
Beta strandi80 – 83Combined sources4
Helixi85 – 88Combined sources4
Beta strandi95 – 97Combined sources3
Helixi104 – 116Combined sources13
Beta strandi122 – 125Combined sources4
Helixi128 – 139Combined sources12
Helixi144 – 157Combined sources14
Turni161 – 165Combined sources5
Helixi168 – 182Combined sources15
Helixi189 – 196Combined sources8
Helixi202 – 212Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K1FX-ray4.30M1-68[»]
3K7AX-ray3.80M1-345[»]
4BBRX-ray3.40M1-345[»]
4BBSX-ray3.60M1-345[»]
4V1Nelectron microscopy7.80M1-345[»]
4V1Oelectron microscopy9.70M1-345[»]
5FMFelectron microscopy6.00P1-345[»]
5FYWelectron microscopy4.35M1-345[»]
5FZ5electron microscopy8.80M1-345[»]
5SVAelectron microscopy15.30c1-345[»]
ProteinModelPortaliP29055.
SMRiP29055.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29055.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati136 – 2121Add BLAST77
Repeati242 – 3182Add BLAST77

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri20 – 53TFIIB-typePROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000006671.
HOGENOMiHOG000108634.
InParanoidiP29055.
KOiK03124.
OMAiSMESIMA.
OrthoDBiEOG092C36CN.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 1 hit.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL
60 70 80 90 100
VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE
110 120 130 140 150
TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA
160 170 180 190 200
KEAYKLCHDE KTLKGKSMES IMAASILIGC RRAEVARTFK EIQSLIHVKT
210 220 230 240 250
KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP RFCSHLGLPM
260 270 280 290 300
QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ
310 320 330 340
TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK
Length:345
Mass (Da):38,200
Last modified:December 1, 1992 - v1
Checksum:i8F1F6D24602436E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10R → L in AAT93251 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81380 Genomic DNA. Translation: AAA35126.1.
U51033 Genomic DNA. Translation: AAB68135.1.
AY693232 Genomic DNA. Translation: AAT93251.1.
BK006949 Genomic DNA. Translation: DAA11504.1.
PIRiS26707.
RefSeqiNP_015411.1. NM_001184183.1.

Genome annotation databases

EnsemblFungiiYPR086W; YPR086W; YPR086W.
GeneIDi856201.
KEGGisce:YPR086W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81380 Genomic DNA. Translation: AAA35126.1.
U51033 Genomic DNA. Translation: AAB68135.1.
AY693232 Genomic DNA. Translation: AAT93251.1.
BK006949 Genomic DNA. Translation: DAA11504.1.
PIRiS26707.
RefSeqiNP_015411.1. NM_001184183.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K1FX-ray4.30M1-68[»]
3K7AX-ray3.80M1-345[»]
4BBRX-ray3.40M1-345[»]
4BBSX-ray3.60M1-345[»]
4V1Nelectron microscopy7.80M1-345[»]
4V1Oelectron microscopy9.70M1-345[»]
5FMFelectron microscopy6.00P1-345[»]
5FYWelectron microscopy4.35M1-345[»]
5FZ5electron microscopy8.80M1-345[»]
5SVAelectron microscopy15.30c1-345[»]
ProteinModelPortaliP29055.
SMRiP29055.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36257. 76 interactors.
DIPiDIP-701N.
IntActiP29055. 57 interactors.
MINTiMINT-473341.

Proteomic databases

MaxQBiP29055.
PRIDEiP29055.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR086W; YPR086W; YPR086W.
GeneIDi856201.
KEGGisce:YPR086W.

Organism-specific databases

EuPathDBiFungiDB:YPR086W.
SGDiS000006290. SUA7.

Phylogenomic databases

GeneTreeiENSGT00390000006671.
HOGENOMiHOG000108634.
InParanoidiP29055.
KOiK03124.
OMAiSMESIMA.
OrthoDBiEOG092C36CN.

Enzyme and pathway databases

BioCyciYEAST:G3O-34230-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

EvolutionaryTraceiP29055.
PROiP29055.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 1 hit.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF2B_YEAST
AccessioniPrimary (citable) accession number: P29055
Secondary accession number(s): D6W488, E9P939
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.