P29055 (TF2B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor IIB

Alternative name(s):
General transcription factor TFIIB
Transcription factor E

Gene names

Name:	SUA7
Ordered Locus Names:	YPR086W
ORF Names:	P9513.4

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.
Subcellular location	Nucleus.
Miscellaneous	Present with 6750 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription regulatory region DNA binding Inferred from direct assay PubMed 8132551. Source: SGD transcriptional open complex formation at RNA polymerase II promoter Inferred from mutant phenotype PubMed 19820686. Source: SGD transcriptional start site selection at RNA polymerase II promoter Inferred from mutant phenotype PubMed 19820686. Source: SGD
Cellular_component	nucleus Inferred from direct assay PubMed 15888545. Source: SGD
Molecular_function	RNA polymerase II core binding Inferred from direct assay PubMed 7935466. Source: SGD TBP-class protein binding Inferred from direct assay PubMed 15888545. Source: SGD TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly Inferred from direct assay PubMed 10464320. Source: SGD core RNA polymerase II recruiting transcription factor activity Inferred from direct assay PubMed 10464320. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 345

345

Transcription initiation factor IIB

PRO_0000119308

Regions

Repeat

136 – 212

77

1

Repeat

242 – 318

77

2

Zinc finger

20 – 53

34

TFIIB-type

Sites

Metal binding

24

1

Zinc By similarity

Metal binding

27

1

Zinc By similarity

Metal binding

45

1

Zinc By similarity

Metal binding

48

1

Zinc By similarity

Experimental info

Sequence conflict

10

1

R → L in AAT93251. Ref.4

Secondary structure

1

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345

Helix Strand Turn

Details...

Beta strand

34 – 37

4

Turn

38 – 41

4

Beta strand

42 – 45

4

Turn

46 – 48

3

Beta strand

50 – 54

5

Helix

59 – 63

5

Turn

64 – 66

3

Beta strand

71 – 73

3

Beta strand

80 – 83

4

Helix

85 – 88

4

Beta strand

95 – 97

3

Helix

104 – 116

13

Beta strand

122 – 125

4

Helix

128 – 139

12

Helix

144 – 157

14

Turn

161 – 165

5

Helix

168 – 182

15

Helix

189 – 196

8

Helix

202 – 212

11

Sequences

	Sequence		Length	Mass (Da)
	P29055 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 8F1F6D24602436E2	FASTA	345	38,200
	10 20 30 40 50 60 MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR 70 80 90 100 110 120 SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE TTDMRFTKEL NKAQGKNVMD 130 140 150 160 170 180 KKDNEVQAAF AKITMLCDAA ELPKIVKDCA KEAYKLCHDE KTLKGKSMES IMAASILIGC 190 200 210 220 230 240 RRAEVARTFK EIQSLIHVKT KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP 250 260 270 280 290 300 RFCSHLGLPM QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ 310 320 330 340 TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo." Pinto I., Ware D.E., Hampsey M. Cell 68:977-988(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M81380 Genomic DNA. Translation: AAA35126.1.
U51033 Genomic DNA. Translation: AAB68135.1.
AY693232 Genomic DNA. Translation: AAT93251.1.
BK006949 Genomic DNA. Translation: DAA11504.1.

PIR

S26707.

RefSeq

NP_015411.1. NM_001184183.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3K1F	X-ray	4.30	M	1-68	[»]
3K7A	X-ray	3.80	M	1-345	[»]
4BBR	X-ray	3.40	M	1-345	[»]
4BBS	X-ray	3.60	M	1-345	[»]

ProteinModelPortal

P29055.

SMR

P29055. Positions 22-317.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-701N.

IntAct

P29055. 54 interactions.

MINT

MINT-473341.

STRING

4932.YPR086W.

Proteomic databases

PaxDb

P29055.

PeptideAtlas

P29055.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YPR086W; YPR086W; YPR086W.

GeneID

856201.

KEGG

sce:YPR086W.

Organism-specific databases

CYGD

YPR086w.

SGD

S000006290. SUA7.

Phylogenomic databases

eggNOG

COG1405.

GeneTree

ENSGT00390000006671.

HOGENOM

HOG000108634.

KO

K03124.

OMA

SNDDQNG.

OrthoDB

EOG4RZ27R.

Gene expression databases

Genevestigator

P29055.

GermOnline

YPR086W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.472.10. 2 hits.

InterPro

IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]

PANTHER

PTHR11618. PTHR11618. 1 hit.

Pfam

PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]

PRINTS

PR00685. TIFACTORIIB.

SMART

SM00385. CYCLIN. 2 hits.
[Graphical view]

SUPFAM

SSF47954. Cyclin_like. 2 hits.

PROSITE

PS00782. TFIIB. 1 hit.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P29055.

NextBio

981402.

Entry information

Entry name

TF2B_YEAST

Accession

Primary (citable) accession number: P29055
Secondary accession number(s): D6W488, E9P939

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program