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P29055

- TF2B_YEAST

UniProt

P29055 - TF2B_YEAST

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Protein
Transcription initiation factor IIB
Gene
SUA7, YPR086W, P9513.4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Zinc By similarity
Metal bindingi27 – 271Zinc By similarity
Metal bindingi45 – 451Zinc By similarity
Metal bindingi48 – 481Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri20 – 5334TFIIB-type
Add
BLAST

GO - Molecular functioni

  1. RNA polymerase II core binding Source: SGD
  2. TBP-class protein binding Source: SGD
  3. TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly Source: SGD
  4. core RNA polymerase II recruiting transcription factor activity Source: SGD
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  2. positive regulation of transcription regulatory region DNA binding Source: SGD
  3. transcriptional open complex formation at RNA polymerase II promoter Source: SGD
  4. transcriptional start site selection at RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34230-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIB
Alternative name(s):
General transcription factor TFIIB
Transcription factor E
Gene namesi
Name:SUA7
Ordered Locus Names:YPR086W
ORF Names:P9513.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR086w.
SGDiS000006290. SUA7.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Transcription initiation factor IIB
PRO_0000119308Add
BLAST

Proteomic databases

MaxQBiP29055.
PaxDbiP29055.
PeptideAtlasiP29055.

Expressioni

Gene expression databases

GenevestigatoriP29055.

Interactioni

Subunit structurei

Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.

Protein-protein interaction databases

BioGridi36257. 75 interactions.
DIPiDIP-701N.
IntActiP29055. 56 interactions.
MINTiMINT-473341.
STRINGi4932.YPR086W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 374
Turni38 – 414
Beta strandi42 – 454
Turni46 – 483
Beta strandi50 – 545
Helixi59 – 635
Turni64 – 663
Beta strandi71 – 733
Beta strandi80 – 834
Helixi85 – 884
Beta strandi95 – 973
Helixi104 – 11613
Beta strandi122 – 1254
Helixi128 – 13912
Helixi144 – 15714
Turni161 – 1655
Helixi168 – 18215
Helixi189 – 1968
Helixi202 – 21211

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K1FX-ray4.30M1-68[»]
3K7AX-ray3.80M1-345[»]
4BBRX-ray3.40M1-345[»]
4BBSX-ray3.60M1-345[»]
ProteinModelPortaliP29055.
SMRiP29055. Positions 22-317.

Miscellaneous databases

EvolutionaryTraceiP29055.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati136 – 212771
Add
BLAST
Repeati242 – 318772
Add
BLAST

Sequence similaritiesi

Belongs to the TFIIB family.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG1405.
GeneTreeiENSGT00390000006671.
HOGENOMiHOG000108634.
KOiK03124.
OMAiEYTERES.
OrthoDBiEOG7RV9S3.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 1 hit.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29055-1 [UniParc]FASTAAdd to Basket

« Hide

MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL    50
VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE 100
TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA 150
KEAYKLCHDE KTLKGKSMES IMAASILIGC RRAEVARTFK EIQSLIHVKT 200
KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP RFCSHLGLPM 250
QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ 300
TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK 345
Length:345
Mass (Da):38,200
Last modified:December 1, 1992 - v1
Checksum:i8F1F6D24602436E2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101R → L in AAT93251. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M81380 Genomic DNA. Translation: AAA35126.1.
U51033 Genomic DNA. Translation: AAB68135.1.
AY693232 Genomic DNA. Translation: AAT93251.1.
BK006949 Genomic DNA. Translation: DAA11504.1.
PIRiS26707.
RefSeqiNP_015411.1. NM_001184183.1.

Genome annotation databases

EnsemblFungiiYPR086W; YPR086W; YPR086W.
GeneIDi856201.
KEGGisce:YPR086W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M81380 Genomic DNA. Translation: AAA35126.1 .
U51033 Genomic DNA. Translation: AAB68135.1 .
AY693232 Genomic DNA. Translation: AAT93251.1 .
BK006949 Genomic DNA. Translation: DAA11504.1 .
PIRi S26707.
RefSeqi NP_015411.1. NM_001184183.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3K1F X-ray 4.30 M 1-68 [» ]
3K7A X-ray 3.80 M 1-345 [» ]
4BBR X-ray 3.40 M 1-345 [» ]
4BBS X-ray 3.60 M 1-345 [» ]
ProteinModelPortali P29055.
SMRi P29055. Positions 22-317.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36257. 75 interactions.
DIPi DIP-701N.
IntActi P29055. 56 interactions.
MINTi MINT-473341.
STRINGi 4932.YPR086W.

Proteomic databases

MaxQBi P29055.
PaxDbi P29055.
PeptideAtlasi P29055.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR086W ; YPR086W ; YPR086W .
GeneIDi 856201.
KEGGi sce:YPR086W.

Organism-specific databases

CYGDi YPR086w.
SGDi S000006290. SUA7.

Phylogenomic databases

eggNOGi COG1405.
GeneTreei ENSGT00390000006671.
HOGENOMi HOG000108634.
KOi K03124.
OMAi EYTERES.
OrthoDBi EOG7RV9S3.

Enzyme and pathway databases

BioCyci YEAST:G3O-34230-MONOMER.

Miscellaneous databases

EvolutionaryTracei P29055.
NextBioi 981402.
PROi P29055.

Gene expression databases

Genevestigatori P29055.

Family and domain databases

Gene3Di 1.10.472.10. 2 hits.
InterProi IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view ]
PANTHERi PTHR11618. PTHR11618. 1 hit.
Pfami PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view ]
PRINTSi PR00685. TIFACTORIIB.
SMARTi SM00385. CYCLIN. 2 hits.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 2 hits.
PROSITEi PS00782. TFIIB. 1 hit.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo."
    Pinto I., Ware D.E., Hampsey M.
    Cell 68:977-988(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTF2B_YEAST
AccessioniPrimary (citable) accession number: P29055
Secondary accession number(s): D6W488, E9P939
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 3, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6750 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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