Reviewed, UniProtKB/Swiss-Prot P29051 (DHE41_HALSA)

Names and origin

Protein names

Recommended name:
    NAD-specific glutamate dehydrogenase A
      Short name=NAD-GDH A
    EC=1.4.1.2

Gene names

Name:	gdhA
Synonyms:	gdhX

Organism

Halobacterium salinarium (Halobacterium halobium)

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	NAD-specific glutamate dehydrogenase A		PRO_0000182779
Sites
	Active site	126	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29051-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 0E4E940D2FF8B9D2	FASTA	435	47,459
	10 20 30 40 50 60 MTMASKSDST HDESGDEAAD STEPESALET ARRQLYHAAS YLDIDQNIVE RLKYPKKVHE 70 80 90 100 110 120 VTIPIERDDG TVEVFTGYRA QHDSVRGPYK GGLRYHPDVT RDECVGLGMW MTWKCAVMDL 130 140 150 160 170 180 PFGGAKGGVA VNPKELSPEE KERLTRRFTQ EIRDVIGPNQ DIPAPDMGTD PQTMAWLMDA 190 200 210 220 230 240 YSMQEGETTP GVVTGKPPVV GGSEGREEAP GRSVAIITQL VCEYYDQPLD ETTVAVQGYG 250 260 270 280 290 300 SVGANAARLL DKWGATIVAI SDVNGAMYEP DGIDTASVPS HDEEPEAVTT YADTVISNEE 310 320 330 340 350 360 LLTLDVDVLI PAALGNVITK ENAEAIAADL VVEGANGPTT STADSILADR DVAVIPDILA 370 380 390 400 410 420 NAGGVTVSYF EWLQDINRRA WSLERVNDEL EAEMQAAWRA VKDEYENRDV TWRDAAYIVA 430 LSRIAEAHEA RGLWP

References

[1]	"The gene for a halophilic glutamate dehydrogenase: sequence, transcription analysis and phylogenetic implications." Benachenhou N., Baldacci G. Mol. Gen. Genet. 230:345-352(1991) [PubMed: 1766432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CCM 2090 / JCM 9120.
[2]	"The discovery of four distinct glutamate dehydrogenase genes in a strain of Halobacterium salinarum." Ingoldsby L.M., Geoghegan K.F., Hayden B.M., Engel P.C. Gene 349:237-244(2005) [PubMed: 15780999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRC-36014.
[3]	"Glutamate dehydrogenase of Halobacterium salinarum: evidence that the gene sequence currently assigned to the NADP+-dependent enzyme is in fact that of the NAD+-dependent glutamate dehydrogenase." Hayden B.M., Bonete M.J., Brown P.E., Moir A.J., Engel P.C. FEMS Microbiol. Lett. 211:37-41(2002) [PubMed: 12052548] [Abstract] Cited for: PROTEIN SEQUENCE OF 119-128; 184-193 AND 268-276, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63837 Genomic DNA. Translation: CAA45327.1. AY840088 Genomic DNA. Translation: AAW19068.1.
PIR	S18609.
3D structure databases
HSSP	HSSP built from PDB template 1B26 based on UniProtKB P96110.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-741.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE41_HALSA

Accession

Primary (citable) accession number: P29051

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)