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Protein

TATA-box-binding protein

Gene

Tbp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-427359. SIRT1 negatively regulates rRNA Expression.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73776. RNA Polymerase II Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-MMU-73863. RNA Polymerase I Transcription Termination.
R-MMU-75953. RNA Polymerase II Transcription Initiation.
R-MMU-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-MMU-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-MMU-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding protein
Alternative name(s):
TATA sequence-binding protein
TATA-binding factor
TATA-box factor
Transcription initiation factor TFIID TBP subunit
Gene namesi
Name:Tbp
Synonyms:Tfiid
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:101838. Tbp.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • female pronucleus Source: MGI
  • male pronucleus Source: MGI
  • nuclear chromatin Source: MGI
  • nuclear euchromatin Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • pronucleus Source: MGI
  • RNA polymerase I transcription factor complex Source: MGI
  • transcription factor TFIIA complex Source: MGI
  • transcription factor TFIID complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539581 – 316TATA-box-binding proteinAdd BLAST316

Proteomic databases

EPDiP29037.
PaxDbiP29037.
PeptideAtlasiP29037.
PRIDEiP29037.

PTM databases

iPTMnetiP29037.
PhosphoSitePlusiP29037.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000014767.
CleanExiMM_TBP.
ExpressionAtlasiP29037. baseline and differential.
GenevisibleiP29037. MM.

Interactioni

Subunit structurei

Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (By similarity). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C (PubMed:9050847). Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4 (By similarity). Interacts with UTF1 (PubMed:9524124). Interacts with BRF2; this interaction promotes recruitment of BRF2 to TATA box-containing promoters. Interacts with UBTF (By similarity). Interacts with GPBP1 (PubMed:14612417). Interacts with CITED2 (PubMed:10593900). Interacts with ATF7IP (Probable). Interacts with LLPH (PubMed:26961175).By similarityCurated5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203978. 31 interactors.
DIPiDIP-24197N.
IntActiP29037. 7 interactors.
MINTiMINT-4590211.
STRINGi10090.ENSMUSP00000014911.

Structurei

3D structure databases

ProteinModelPortaliP29037.
SMRiP29037.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati142 – 2181Add BLAST77
Repeati232 – 3092Add BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 70Poly-GlnAdd BLAST16

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
GeneTreeiENSGT00410000025389.
HOGENOMiHOG000105164.
HOVERGENiHBG044997.
InParanoidiP29037.
KOiK03120.
OMAiMMPYGSG.
OrthoDBiEOG091G0ENY.
PhylomeDBiP29037.
TreeFamiTF300102.

Family and domain databases

CDDicd04516. TBP_eukaryotes. 1 hit.
Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch. 1 hit.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
IPR033710. TBP_eukaryotic.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS
60 70 80 90 100
ILEEQQRQQQ QQQQQQQQQQ AVATAAASVQ QSTSQQPTQG ASGQTPQLFH
110 120 130 140 150
SQTLTTAPLP GTTPLYPSPM TPMTPITPAT PASESSGIVP QLQNIVSTVN
160 170 180 190 200
LGCKLDLKTI ALRARNAEYN PKRFAAVIMR IREPRTTALI FSSGKMVCTG
210 220 230 240 250
AKSEEQSRLA ARKYARVVQK LGFPAKFLDF KIQNMVGSCD VKFPIRLEGL
260 270 280 290 300
VLTHQQFSSY EPELFPGLIY RMIKPRIVLL IFVSGKVVLT GAKVRAEIYE
310
AFENIYPILK GFRKTT
Length:316
Mass (Da):34,709
Last modified:December 1, 1992 - v1
Checksum:iA33D776160B4A393
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141Q → R in AAH50136 (PubMed:15489334).Curated1
Sequence conflicti164A → P in BAC39346 (PubMed:16141072).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti70Q → QQQ in strain: TW18/TW5. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D01034 mRNA. Translation: BAA00840.1.
U63933 mRNA. Translation: AAB53097.1.
AK085037 mRNA. Translation: BAC39346.1.
BC050136 mRNA. Translation: AAH50136.1.
BC012685 mRNA. Translation: AAH12685.1.
CCDSiCCDS37454.2.
PIRiS34437.
RefSeqiNP_038712.3. NM_013684.3.
UniGeneiMm.244820.

Genome annotation databases

EnsembliENSMUST00000014911; ENSMUSP00000014911; ENSMUSG00000014767.
ENSMUST00000117593; ENSMUSP00000112794; ENSMUSG00000014767.
ENSMUST00000162505; ENSMUSP00000124317; ENSMUSG00000014767.
GeneIDi21374.
KEGGimmu:21374.
UCSCiuc008aon.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D01034 mRNA. Translation: BAA00840.1.
U63933 mRNA. Translation: AAB53097.1.
AK085037 mRNA. Translation: BAC39346.1.
BC050136 mRNA. Translation: AAH50136.1.
BC012685 mRNA. Translation: AAH12685.1.
CCDSiCCDS37454.2.
PIRiS34437.
RefSeqiNP_038712.3. NM_013684.3.
UniGeneiMm.244820.

3D structure databases

ProteinModelPortaliP29037.
SMRiP29037.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203978. 31 interactors.
DIPiDIP-24197N.
IntActiP29037. 7 interactors.
MINTiMINT-4590211.
STRINGi10090.ENSMUSP00000014911.

PTM databases

iPTMnetiP29037.
PhosphoSitePlusiP29037.

Proteomic databases

EPDiP29037.
PaxDbiP29037.
PeptideAtlasiP29037.
PRIDEiP29037.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014911; ENSMUSP00000014911; ENSMUSG00000014767.
ENSMUST00000117593; ENSMUSP00000112794; ENSMUSG00000014767.
ENSMUST00000162505; ENSMUSP00000124317; ENSMUSG00000014767.
GeneIDi21374.
KEGGimmu:21374.
UCSCiuc008aon.2. mouse.

Organism-specific databases

CTDi6908.
MGIiMGI:101838. Tbp.

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
GeneTreeiENSGT00410000025389.
HOGENOMiHOG000105164.
HOVERGENiHBG044997.
InParanoidiP29037.
KOiK03120.
OMAiMMPYGSG.
OrthoDBiEOG091G0ENY.
PhylomeDBiP29037.
TreeFamiTF300102.

Enzyme and pathway databases

ReactomeiR-MMU-427359. SIRT1 negatively regulates rRNA Expression.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73776. RNA Polymerase II Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-MMU-73863. RNA Polymerase I Transcription Termination.
R-MMU-75953. RNA Polymerase II Transcription Initiation.
R-MMU-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-MMU-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-MMU-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

PROiP29037.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000014767.
CleanExiMM_TBP.
ExpressionAtlasiP29037. baseline and differential.
GenevisibleiP29037. MM.

Family and domain databases

CDDicd04516. TBP_eukaryotes. 1 hit.
Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch. 1 hit.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
IPR033710. TBP_eukaryotic.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBP_MOUSE
AccessioniPrimary (citable) accession number: P29037
Secondary accession number(s): O09169, Q80UL8, Q8C3S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.