P29037 (TBP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TATA-box-binding protein

Alternative name(s):
TATA sequence-binding protein
TATA-binding factor
TATA-box factor
Transcription initiation factor TFIID TBP subunit

Gene names

Name:	Tbp
Synonyms:	Tfiid

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA By similarity.
Subunit structure	Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with BRF2. Interacts with UBTF By similarity. Interacts with UTF1. Interacts with GPBP1. Interacts with SP1 By similarity. Interacts with CITED2. Ref.8 Ref.9 Ref.10 Ref.11
Subcellular location	Nucleus.
Tissue specificity	Ubiquitously expressed. Ref.5
Sequence similarities	Belongs to the TBP family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription from RNA polymerase III promoter Inferred from mutant phenotype. Source: MGI transcription initiation from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro
Cellular component	RNA polymerase I transcription factor complex Traceable author statement Ref.8. Source: MGI cytoplasm Inferred from direct assay. Source: MGI female pronucleus Inferred from direct assay. Source: MGI male pronucleus Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	TATA-box-binding protein		PRO_0000153958
Regions
	Repeat	142 – 218	77	1
	Repeat	232 – 309	78	2
	Compositional bias	55 – 70	16	Poly-Gln
Natural variations
	Natural variant	70	1	Q → QQQ in strain: TW18/TW5.
Experimental info
	Sequence conflict	141	1	Q → R in AAH50136. Ref.6
	Sequence conflict	164	1	A → P in BAC39346. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P29037 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: A33D776160B4A393	FASTA	316	34,709
	10 20 30 40 50 60 MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ 70 80 90 100 110 120 QQQQQQQQQQ AVATAAASVQ QSTSQQPTQG ASGQTPQLFH SQTLTTAPLP GTTPLYPSPM 130 140 150 160 170 180 TPMTPITPAT PASESSGIVP QLQNIVSTVN LGCKLDLKTI ALRARNAEYN PKRFAAVIMR 190 200 210 220 230 240 IREPRTTALI FSSGKMVCTG AKSEEQSRLA ARKYARVVQK LGFPAKFLDF KIQNMVGSCD 250 260 270 280 290 300 VKFPIRLEGL VLTHQQFSSY EPELFPGLIY RMIKPRIVLL IFVSGKVVLT GAKVRAEIYE 310 AFENIYPILK GFRKTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Striking homology of the 'variable' N-terminal as well as the 'conserved core' domains of the mouse and human TATA-factors (TFIID)." Tamura T.-A., Sumita K., Fujino I., Aoyama A., Horikoshi M., Hoffmann A., Roeder R.G., Muramatsu M., Mikoshiba K. Nucleic Acids Res. 19:3861-3865(1991) [PubMed: 1861978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of a mammalian TBP (TATA-binding protein) gene: isolation of the mouse TBP genome." Sumita K., Makino Y., Katoh K., Kashimoto T., Muramatsu M., Mikoshiba K., Tamura T.-A. Nucleic Acids Res. 21:2769-2769(1993) [PubMed: 8332475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Linkage of TATA-binding protein and proteasome subunit C5 genes in mice and humans reveals synteny conserved between mammals and invertebrates." Trachtulec Z., Hamvas R.M., Forejt J., Lehrach H.R., Vincek V., Klein J. Genomics 44:1-7(1997) [PubMed: 9286694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: TW18/TW5. Tissue: Testis.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[5]	"Developmental and cell type-specific regulation of core promoter transcription factors in germ cells of frogs and mice." Xiao L., Kim M., DeJong J. Gene Expr. Patterns 6:409-419(2006) [PubMed: 16412700] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: B5/EGFP transgenic ICR mice and FVB/N. Tissue: Mammary tumor and Trophoblast.
[7]	"Promoter structure of the mouse TATA-binding protein (TBP) gene." Ohbayashi T., Schmidt E.E., Makino Y., Kishimoto T., Nabeshima Y., Muramatsu M., Tamura T.-A. Biochem. Biophys. Res. Commun. 225:275-280(1996) [PubMed: 8769130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-36.
[8]	"Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1." Heix J., Zomerdijk J.C.B.M., Ravanpay A., Tjian R., Grummt I. Proc. Natl. Acad. Sci. U.S.A. 94:1733-1738(1997) [PubMed: 9050847] [Abstract] Cited for: INTERACTION WITH TAF1A; TAF1B AND TAF1C.
[9]	"UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells." Okuda A., Fukushima A., Nishimoto M., Orimo A., Yamagishi T., Nabeshima Y., Kuro-o M., Nabeshima Y., Boon K., Keaveney M., Stunnenberg H.G., Muramatsu M. EMBO J. 17:2019-2032(1998) [PubMed: 9524124] [Abstract] Cited for: INTERACTION WITH UTF1.
[10]	"MRG1 binds to the LIM domain of Lhx2 and may function as a coactivator to stimulate glycoprotein hormone alpha-subunit gene expression." Glenn D.J., Maurer R.A. J. Biol. Chem. 274:36159-36167(1999) [PubMed: 10593900] [Abstract] Cited for: INTERACTION WITH CITED2.
[11]	"The murine G+C-rich promoter binding protein mGPBP is required for promoter-specific transcription." Hsu L.-C., Liu S., Abedinpour F., Beech R.D., Lahti J.M., Kidd V.J., Greenspan J.A., Yeung C.-Y. Mol. Cell. Biol. 23:8773-8785(2003) [PubMed: 14612417] [Abstract] Cited for: INTERACTION WITH GPBP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D01034 mRNA. Translation: BAA00840.1. U63933 mRNA. Translation: AAB53097.1. AK085037 mRNA. Translation: BAC39346.1. BC050136 mRNA. Translation: AAH50136.1. BC012685 mRNA. Translation: AAH12685.1.
IPI	IPI00776010.
PIR	S34437.
RefSeq	NP_038712.3. NM_013684.3.
UniGene	Mm.244820.
3D structure databases
ProteinModelPortal	P29037.
SMR	P29037. Positions 136-314.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24197N.
IntAct	P29037. 1 interaction.
STRING	P29037.
PTM databases
PhosphoSite	P29037.
Proteomic databases
PRIDE	P29037.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000014911; ENSMUSP00000014911; ENSMUSG00000014767. ENSMUST00000117593; ENSMUSP00000112794; ENSMUSG00000014767. ENSMUST00000162505; ENSMUSP00000124317; ENSMUSG00000014767.
GeneID	21374.
KEGG	mmu:21374.
Organism-specific databases
CTD	6908.
MGI	MGI:101838. Tbp.
Phylogenomic databases
HOGENOM	HBG499930.
HOVERGEN	HBG044997.
InParanoid	P29037.
OMA	PMMPYGT.
OrthoDB	EOG4PVP0C.
PhylomeDB	P29037.
Gene expression databases
ArrayExpress	P29037.
Bgee	P29037.
CleanEx	MM_TBP.
Genevestigator	P29037.
GermOnline	ENSMUSG00000014767. Mus musculus.
Family and domain databases
InterPro	IPR000814. TBP. IPR012294. TFIID_C/glycos_N. [Graphical view]
KO	K03120.
PANTHER	PTHR10126. TBP. 1 hit.
Pfam	PF00352. TBP. 2 hits. [Graphical view]
PRINTS	PR00686. TIFACTORIID.
SUPFAM	SSF55945. TFIID_C/glycos_N. 2 hits.
PROSITE	PS00351. TFIID. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	300592.
SOURCE	Search...

Entry information

Entry name

TBP_MOUSE

Accession

Primary (citable) accession number: P29037
Secondary accession number(s): O09169, Q80UL8, Q8C3S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 25, 2012
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program