Reviewed, UniProtKB/Swiss-Prot P29037 (TBP_MOUSE)

Names and origin

Protein names

Recommended name:
    TATA-box-binding protein
Alternative name(s):
    TATA-box factor
    TATA-binding factor
    TATA sequence-binding protein
    Transcription initiation factor TFIID TBP subunit

Gene names

Name:	Tbp
Synonyms:	Tfiid

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Subunit structure	Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIFIB complex, composed of TBP and at least TAF1A, TAF1B TAF1C, and TAF3. Binds DNA as monomer. Interacts with TAFs, TFIIA, TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2, SNAPC4 and BRF2 By similarity. Interacts with UTF1 which acts as a coactivator of ATF2 transcriptional activity. Interacts with GPBP1.
Subcellular location	Nucleus.
Tissue specificity	Ubiquitously expressed. Ref.5
Sequence similarities	Belongs to the TBP family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro
Cellular component	RNA polymerase I transcription factor complex Ref.8 Traceable author statement. Source: MGI cytoplasm Inferred from direct assay. Source: MGI female pronucleus Inferred from direct assay. Source: MGI male pronucleus Inferred from direct assay. Source: MGI transcription factor TFIID complex Inferred from electronic annotation. Source: InterPro
Molecular function	RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: InterPro protein binding Ref.9 Inferred from physical interaction. Source: UniProtKB transcription factor activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	TATA-box-binding protein		PRO_0000153958
Regions
	Repeat	142 – 218	77	1
	Repeat	232 – 309	78	2
	Compositional bias	55 – 70	16	Poly-Gln
Natural variations
	Natural variant	70	1	Q → QQQ in strain: TW18/TW5.
Experimental info
	Sequence conflict	141	1	Q → R in AAH50136. Ref.6
	Sequence conflict	164	1	A → P in BAC39346. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P29037-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: A33D776160B4A393	FASTA	316	34,709
	10 20 30 40 50 60 MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ 70 80 90 100 110 120 QQQQQQQQQQ AVATAAASVQ QSTSQQPTQG ASGQTPQLFH SQTLTTAPLP GTTPLYPSPM 130 140 150 160 170 180 TPMTPITPAT PASESSGIVP QLQNIVSTVN LGCKLDLKTI ALRARNAEYN PKRFAAVIMR 190 200 210 220 230 240 IREPRTTALI FSSGKMVCTG AKSEEQSRLA ARKYARVVQK LGFPAKFLDF KIQNMVGSCD 250 260 270 280 290 300 VKFPIRLEGL VLTHQQFSSY EPELFPGLIY RMIKPRIVLL IFVSGKVVLT GAKVRAEIYE 310 AFENIYPILK GFRKTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Striking homology of the 'variable' N-terminal as well as the 'conserved core' domains of the mouse and human TATA-factors (TFIID)." Tamura T.-A., Sumita K., Fujino I., Aoyama A., Horikoshi M., Hoffmann A., Roeder R.G., Muramatsu M., Mikoshiba K. Nucleic Acids Res. 19:3861-3865(1991) [PubMed: 1861978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of a mammalian TBP (TATA-binding protein) gene: isolation of the mouse TBP genome." Sumita K., Makino Y., Katoh K., Kashimoto T., Muramatsu M., Mikoshiba K., Tamura T.-A. Nucleic Acids Res. 21:2769-2769(1993) [PubMed: 8332475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Linkage of TATA-binding protein and proteasome subunit C5 genes in mice and humans reveals synteny conserved between mammals and invertebrates." Trachtulec Z., Hamvas R.M., Forejt J., Lehrach H.R., Vincek V., Klein J. Genomics 44:1-7(1997) [PubMed: 9286694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: TW18/TW5. Tissue: Testis.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[5]	"Developmental and cell type-specific regulation of core promoter transcription factors in germ cells of frogs and mice." Xiao L., Kim M., DeJong J. Gene Expr. Patterns 6:409-419(2006) [PubMed: 16412700] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: B5/EGFP transgenic ICR mice and FVB/N. Tissue: Mammary tumor and Trophoblast.
[7]	"Promoter structure of the mouse TATA-binding protein (TBP) gene." Ohbayashi T., Schmidt E.E., Makino Y., Kishimoto T., Nabeshima Y., Muramatsu M., Tamura T.-A. Biochem. Biophys. Res. Commun. 225:275-280(1996) [PubMed: 8769130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-36.
[8]	"Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1." Heix J., Zomerdijk J.C.B.M., Ravanpay A., Tjian R., Grummt I. Proc. Natl. Acad. Sci. U.S.A. 94:1733-1738(1997) [PubMed: 9050847] [Abstract] Cited for: INTERACTION WITH TAF1A; TAF1B AND TAF1C.
[9]	"UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells." Okuda A., Fukushima A., Nishimoto M., Orimo A., Yamagishi T., Nabeshima Y., Kuro-o M., Nabeshima Y., Boon K., Keaveney M., Stunnenberg H.G., Muramatsu M. EMBO J. 17:2019-2032(1998) [PubMed: 9524124] [Abstract] Cited for: INTERACTION WITH UTF1.
[10]	"The murine G+C-rich promoter binding protein mGPBP is required for promoter-specific transcription." Hsu L.-C., Liu S., Abedinpour F., Beech R.D., Lahti J.M., Kidd V.J., Greenspan J.A., Yeung C.-Y. Mol. Cell. Biol. 23:8773-8785(2003) [PubMed: 14612417] [Abstract] Cited for: INTERACTION WITH GPBP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D01034 mRNA. Translation: BAA00840.1. U63933 mRNA. Translation: AAB53097.1. AK085037 mRNA. Translation: BAC39346.1. BC050136 mRNA. Translation: AAH50136.1. BC012685 mRNA. Translation: AAH12685.1.
IPI	IPI00776010.
PIR	S34437.
RefSeq	NP_038712.3.
UniGene	Mm.244820
3D structure databases
HSSP	HSSP built from PDB template 1TGH based on UniProtKB P20226.
SMR	P29037. Positions 136-314.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:24197N.
Proteomic databases
PRIDE	P29037.
Genome annotation databases
Ensembl	ENSMUSG00000014767. Mus musculus. [Contig view]
GeneID	21374.
KEGG	mmu:21374.
Organism-specific databases
MGI	MGI:101838. Tbp.
Phylogenomic databases
HOGENOM	P29037.
HOVERGEN	P29037.
OMA	P29037. PMMPYGT.
Gene expression databases
ArrayExpress	P29037.
Bgee	P29037.
CleanEx	MM_TBP.
GermOnline	ENSMUSG00000014767. Mus musculus.
Family and domain databases
InterPro	IPR012295. b_Adaptin_TBP_C. IPR000814. TBP. [Graphical view]
Gene3D	G3DSA:3.30.310.10. b_Adaptin_TBP_C. 2 hits.
PANTHER	PTHR10126. TBP. 1 hit.
Pfam	PF00352. TBP. 2 hits. [Graphical view]
PRINTS	PR00686. TIFACTORIID.
PROSITE	PS00351. TFIID. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	300592.
SOURCE	Search...

Entry information

Entry name

TBP_MOUSE

Accession

Primary (citable) accession number: P29037
Secondary accession number(s): O09169, Q80UL8, Q8C3S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)