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P29037 (TBP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
TATA-box-binding protein
Alternative name(s):
TATA sequence-binding protein
TATA-binding factor
TATA-box factor
Transcription initiation factor TFIID TBP subunit
Gene names
Name:Tbp
Synonyms:Tfiid
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA By similarity. HAMAP-Rule MF_00408

Subunit structure

Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with BRF2. Interacts with UBTF By similarity. Interacts with UTF1. Interacts with GPBP1. Interacts with SP1 By similarity. Interacts with CITED2. Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location

Nucleus HAMAP-Rule MF_00408.

Tissue specificity

Ubiquitously expressed. Ref.5

Sequence similarities

Belongs to the TBP family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA-templated transcription, initiation

Inferred from electronic annotation. Source: InterPro

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

spermatogenesis

Inferred from electronic annotation. Source: Ensembl

transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 12411709. Source: MGI

transcription from RNA polymerase III promoter

Inferred from mutant phenotype PubMed 12411709. Source: MGI

   Cellular_componentRNA polymerase I transcription factor complex

Traceable author statement Ref.8. Source: MGI

cytoplasm

Inferred from direct assay PubMed 12665565. Source: MGI

female pronucleus

Inferred from direct assay PubMed 17641088. Source: MGI

male pronucleus

Inferred from direct assay PubMed 17641088. Source: MGI

nuclear euchromatin

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 11861477PubMed 12411709PubMed 12471023PubMed 12665565PubMed 15927180PubMed 16522640PubMed 17242199PubMed 17641088PubMed 18722179PubMed 22323595. Source: MGI

pronucleus

Inferred from direct assay PubMed 16822332. Source: MGI

transcription factor TFIIA complex

Inferred from electronic annotation. Source: Ensembl

transcription factor TFIID complex

Inferred from sequence orthology PubMed 14580349. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 15601870PubMed 18218637PubMed 18722179. Source: MGI

RNA polymerase II repressing transcription factor binding

Inferred from physical interaction PubMed 19235719. Source: MGI

protein binding

Inferred from physical interaction Ref.10Ref.9. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316TATA-box-binding protein HAMAP-Rule MF_00408
PRO_0000153958

Regions

Repeat142 – 218771 HAMAP-Rule MF_00408
Repeat232 – 309782 HAMAP-Rule MF_00408
Compositional bias55 – 7016Poly-Gln HAMAP-Rule MF_00408

Natural variations

Natural variant701Q → QQQ in strain: TW18/TW5.

Experimental info

Sequence conflict1411Q → R in AAH50136. Ref.6
Sequence conflict1641A → P in BAC39346. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P29037 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: A33D776160B4A393

FASTA31634,709
        10         20         30         40         50         60 
MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ 

        70         80         90        100        110        120 
QQQQQQQQQQ AVATAAASVQ QSTSQQPTQG ASGQTPQLFH SQTLTTAPLP GTTPLYPSPM 

       130        140        150        160        170        180 
TPMTPITPAT PASESSGIVP QLQNIVSTVN LGCKLDLKTI ALRARNAEYN PKRFAAVIMR 

       190        200        210        220        230        240 
IREPRTTALI FSSGKMVCTG AKSEEQSRLA ARKYARVVQK LGFPAKFLDF KIQNMVGSCD 

       250        260        270        280        290        300 
VKFPIRLEGL VLTHQQFSSY EPELFPGLIY RMIKPRIVLL IFVSGKVVLT GAKVRAEIYE 

       310 
AFENIYPILK GFRKTT 

« Hide

References

« Hide 'large scale' references
[1]"Striking homology of the 'variable' N-terminal as well as the 'conserved core' domains of the mouse and human TATA-factors (TFIID)."
Tamura T.-A., Sumita K., Fujino I., Aoyama A., Horikoshi M., Hoffmann A., Roeder R.G., Muramatsu M., Mikoshiba K.
Nucleic Acids Res. 19:3861-3865(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure of a mammalian TBP (TATA-binding protein) gene: isolation of the mouse TBP genome."
Sumita K., Makino Y., Katoh K., Kashimoto T., Muramatsu M., Mikoshiba K., Tamura T.-A.
Nucleic Acids Res. 21:2769-2769(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Linkage of TATA-binding protein and proteasome subunit C5 genes in mice and humans reveals synteny conserved between mammals and invertebrates."
Trachtulec Z., Hamvas R.M., Forejt J., Lehrach H.R., Vincek V., Klein J.
Genomics 44:1-7(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: TW18/TW5.
Tissue: Testis.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Lung.
[5]"Developmental and cell type-specific regulation of core promoter transcription factors in germ cells of frogs and mice."
Xiao L., Kim M., DeJong J.
Gene Expr. Patterns 6:409-419(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: B5/EGFP transgenic ICR mice and FVB/N.
Tissue: Mammary tumor and Trophoblast.
[7]"Promoter structure of the mouse TATA-binding protein (TBP) gene."
Ohbayashi T., Schmidt E.E., Makino Y., Kishimoto T., Nabeshima Y., Muramatsu M., Tamura T.-A.
Biochem. Biophys. Res. Commun. 225:275-280(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-36.
[8]"Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1."
Heix J., Zomerdijk J.C.B.M., Ravanpay A., Tjian R., Grummt I.
Proc. Natl. Acad. Sci. U.S.A. 94:1733-1738(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TAF1A; TAF1B AND TAF1C.
[9]"UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells."
Okuda A., Fukushima A., Nishimoto M., Orimo A., Yamagishi T., Nabeshima Y., Kuro-o M., Nabeshima Y., Boon K., Keaveney M., Stunnenberg H.G., Muramatsu M.
EMBO J. 17:2019-2032(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UTF1.
[10]"MRG1 binds to the LIM domain of Lhx2 and may function as a coactivator to stimulate glycoprotein hormone alpha-subunit gene expression."
Glenn D.J., Maurer R.A.
J. Biol. Chem. 274:36159-36167(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CITED2.
[11]"The murine G+C-rich promoter binding protein mGPBP is required for promoter-specific transcription."
Hsu L.-C., Liu S., Abedinpour F., Beech R.D., Lahti J.M., Kidd V.J., Greenspan J.A., Yeung C.-Y.
Mol. Cell. Biol. 23:8773-8785(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GPBP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D01034 mRNA. Translation: BAA00840.1.
U63933 mRNA. Translation: AAB53097.1.
AK085037 mRNA. Translation: BAC39346.1.
BC050136 mRNA. Translation: AAH50136.1.
BC012685 mRNA. Translation: AAH12685.1.
CCDSCCDS37454.2.
PIRS34437.
RefSeqNP_038712.3. NM_013684.3.
UniGeneMm.244820.

3D structure databases

ProteinModelPortalP29037.
SMRP29037. Positions 136-314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203978. 30 interactions.
DIPDIP-24197N.
IntActP29037. 7 interactions.
MINTMINT-4590211.

PTM databases

PhosphoSiteP29037.

Proteomic databases

PaxDbP29037.
PRIDEP29037.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000014911; ENSMUSP00000014911; ENSMUSG00000014767.
ENSMUST00000117593; ENSMUSP00000112794; ENSMUSG00000014767.
ENSMUST00000162505; ENSMUSP00000124317; ENSMUSG00000014767.
GeneID21374.
KEGGmmu:21374.
UCSCuc008aon.2. mouse.

Organism-specific databases

CTD6908.
MGIMGI:101838. Tbp.

Phylogenomic databases

eggNOGCOG2101.
HOGENOMHOG000105164.
HOVERGENHBG044997.
InParanoidP29037.
KOK03120.
OMAGQAPQLF.
PhylomeDBP29037.
TreeFamTF300102.

Gene expression databases

ArrayExpressP29037.
BgeeP29037.
CleanExMM_TBP.
GenevestigatorP29037.

Family and domain databases

Gene3D3.30.310.10. 2 hits.
HAMAPMF_00408. TATA_bind_prot_arch.
InterProIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
[Graphical view]
PANTHERPTHR10126. PTHR10126. 1 hit.
PfamPF00352. TBP. 2 hits.
[Graphical view]
PRINTSPR00686. TIFACTORIID.
PROSITEPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio300592.
PROP29037.
SOURCESearch...

Entry information

Entry nameTBP_MOUSE
AccessionPrimary (citable) accession number: P29037
Secondary accession number(s): O09169, Q80UL8, Q8C3S1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot