P29036 (FRI1_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferritin-1, chloroplastic
EC=1.16.3.1

Alternative name(s):
ZmFer1

Gene names

Name:

FER1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
Catalytic activity	4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.
Subunit structure	Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.
Subcellular location	Plastid › chloroplast. Plastid.
Tissue specificity	Ferritins accumulate in seed during maturation. Then, they are degraded during the first days of germination. Present in roots and leaves after iron treatment.
Induction	By iron.
Sequence similarities	Belongs to the ferritin family. Contains 1 ferritin-like diiron domain.
Sequence caution	The sequence CAA43663.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Iron storage
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from electronic annotation. Source: EnsemblPlants/Gramene iron ion transport Inferred from electronic annotation. Source: InterPro leaf development Inferred from electronic annotation. Source: EnsemblPlants/Gramene photosynthesis Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to abscisic acid stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to bacterium Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cold Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to hydrogen peroxide Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to iron ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to zinc ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast stroma Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast thylakoid membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene mitochondrion Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro ferroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Chloroplast Ref.1
	Chain	47 – 254	208	Ferritin-1, chloroplastic		PRO_0000008859
Regions
	Domain	84 – 237	154	Ferritin-like diiron
	Region	47 – 83	37	Extension peptide (EP)
Sites
	Metal binding	101	1	Iron 1 By similarity
	Metal binding	136	1	Iron 1 By similarity
	Metal binding	136	1	Iron 2 By similarity
	Metal binding	139	1	Iron 1 By similarity
	Metal binding	185	1	Iron 2 By similarity
	Metal binding	219	1	Iron 2 By similarity
Experimental info
	Sequence conflict	32	1	Missing in CAA58146. Ref.2
	Sequence conflict	214	1	E → D in CAA58146. Ref.2
	Sequence conflict	220	1	G → V in CAA58146. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P29036 [UniParc]. Last modified April 11, 2003. Version 2. Checksum: 7B74EBB843DBA28D	FASTA	254	28,025
	10 20 30 40 50 60 MMLRVSPSPA AAVPTQLSGA PATPAPVVRV AAPRGVASPS AGAACRAAGK GKEVLSGVVF 70 80 90 100 110 120 QPFEEIKGEL ALVPQSPDKS LARHKFVDDC EAALNEQINV EYNASYAYHS LFAYFDRDNV 130 140 150 160 170 180 ALKGFAKFFK ESSDEEREHA EKLMEYQNKR GGRVRLQSIV TPLTEFDHPE KGDALYAMEL 190 200 210 220 230 240 ALALEKLVNE KLHNLHGVAT RCNDPQLTDF IESEFLEEQG EAINKISKYV AQLRRVGKGH 250 GVWHFDQMLL EEEA

References

[1]	"Iron induces ferritin synthesis in maize plantlets." Lobreaux S., Massenet O., Briat J.-F. Plant Mol. Biol. 19:563-575(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 47-75. Strain: cv. Missouri 17. Tissue: Root and Seed.
[2]	"Structure and differential expression of two maize ferritin genes in response to iron and abscisic acid." Fobis-Loisy I., Loridon K., Lobreaux S., Lebrun M., Briat J.-F. Eur. J. Biochem. 231:609-619(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wisconsin 22. Tissue: Seedling.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61391 mRNA. Translation: CAA43663.1. Different initiation. X83076 Genomic DNA. Translation: CAA58146.1.
PIR	S22498.
RefSeq	NP_001105563.1. NM_001112093.1.
UniGene	Zm.99.
3D structure databases
ProteinModelPortal	P29036.
ModBase	Search...
Proteomic databases
PRIDE	P29036.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542553.
KEGG	zma:542553.
Organism-specific databases
Gramene	P29036.
MaizeGDB	25278.
Phylogenomic databases
HOGENOM	HOG000223383.
KO	K00522.
Family and domain databases
Gene3D	1.20.1260.10. 1 hit.
InterPro	IPR001519. Ferritin. IPR009040. Ferritin-like_diiron. IPR009078. Ferritin-like_SF. IPR012347. Ferritin-rel. IPR014034. Ferritin_CS. IPR008331. Ferritin_DPS_dom. [Graphical view]
PANTHER	PTHR11431. PTHR11431. 1 hit.
Pfam	PF00210. Ferritin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00540. FERRITIN_1. 1 hit. PS00204. FERRITIN_2. 1 hit. PS50905. FERRITIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRI1_MAIZE

Accession

Primary (citable) accession number: P29036
Secondary accession number(s): Q43258

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	April 11, 2003
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program