Reviewed, UniProtKB/Swiss-Prot P29032 (CHIC_POPTR)

Names and origin

Protein names	Recommended name:     Acidic endochitinase WIN6.2C     EC=3.2.1.14
Organism	Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic identifier	3694 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	121 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Induction	By wounding.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – ›121	›99	Acidic endochitinase WIN6.2C		PRO_0000005318
Regions
	Domain	23 – 63	41	Chitin-binding type-1
	Region	78 – 121	44	Catalytic
	Compositional bias	64 – 77	14	Gly-rich
Amino acid modifications
	Disulfide bond	25 ↔ 40		By similarity
	Disulfide bond	34 ↔ 46		By similarity
	Disulfide bond	39 ↔ 53		By similarity
	Disulfide bond	57 ↔ 61		By similarity
Experimental info
	Non-terminal residue	121	1

Sequences

	Sequence		Length	Mass (Da)
	P29032-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: A89DFC96795FE87F	FASTA	121	12,689
	10 20 30 40 50 60 MSVWAFFAFF SLFLSLSVRG SAEQCGRQAG DALCPGGLCC SFYGWCGTTV DYCGDGCQSQ 70 80 90 100 110 120 CDGGDGCDGG GGGGGDGDDG YLSDIIPKST FDALLKFRND PRCHAVGFYT YDAFISAAKE F

References

[1]	"Populus chitinase genes: structure, organization, and similarity of translated sequences to herbaceous plant chitinases." Davis J.M., Clarke H.R.G., Bradshaw H.D. Jr., Gordon M.P. Plant Mol. Biol. 17:631-639(1991) [PubMed: 1912489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59995 Genomic DNA. Translation: CAA42614.1.
3D structure databases
HSSP	HSSP built from PDB template 1HEV based on UniProtKB P02877.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 262297.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. Partial match. PS00774. CHITINASE_19_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIC_POPTR

Accession

Primary (citable) accession number: P29032

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	September 22, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)