Reviewed, UniProtKB/Swiss-Prot P29031 (CHIB_POPTR)

Names and origin

Protein names	Recommended name:     Acidic endochitinase WIN6.2B     EC=3.2.1.14
Organism	Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic identifier	3694 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Induction	By wounding.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity
	Chain	22 – 303	282	Acidic endochitinase WIN6.2B		PRO_0000005317
Regions
	Domain	22 – 62	41	Chitin-binding type-1
	Region	82 – 303	222	Chitinase
Amino acid modifications
	Disulfide bond	24 ↔ 39		By similarity
	Disulfide bond	33 ↔ 45		By similarity
	Disulfide bond	38 ↔ 52		By similarity
	Disulfide bond	56 ↔ 60		By similarity
	Disulfide bond	253 ↔ 286		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29031-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 0E0D011DFA5CC8B8	FASTA	303	32,209
	10 20 30 40 50 60 MSVWAFAFFS LFLSLSVRGS AEQCGQQAGD ALCPGGLCCS SYGWCGTTAD YCGDGCQSQC 70 80 90 100 110 120 DGGGGGGGGG GGGGGGGGGG GDGYLSDIIP ESMFDDMLKY RNDPQCPAVG FYTYNAFISA 130 140 150 160 170 180 AKEFPDFGNT GDDLMRKREI AAFLGQTSHE TNGWWPAAQG DQYDWGYCHM NYNYGLCGDD 190 200 210 220 230 240 LNLPLLQEPE LVETDPFIAF KTALWFWMTP QSPKPSCHAV ITESWTPSAA DSEAGRVPGY 250 260 270 280 290 300 GVITNIINGG IECGQGGPNN ANENRIGFYK TYCDSLGTTY GSNLDCYQQR PFGYGLLGLK DTM

References

[1]	"Populus chitinase genes: structure, organization, and similarity of translated sequences to herbaceous plant chitinases." Davis J.M., Clarke H.R.G., Bradshaw H.D. Jr., Gordon M.P. Plant Mol. Biol. 17:631-639(1991) [PubMed: 1912489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59995 Genomic DNA. Translation: CAA42612.1.
3D structure databases
HSSP	HSSP built from PDB template 1HEV based on UniProtKB P02877.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 262297.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIB_POPTR

Accession

Primary (citable) accession number: P29031

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1996
Last modified:	September 22, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)