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P29030

- CHIT_BRUMA

UniProt

P29030 - CHIT_BRUMA

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Protein

Endochitinase

Gene
N/A
Organism
Brugia malayi (Filarial nematode worm)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Microfilarial chitinase, which may function to degrade chitin-containing structures in the micro-filaria or in its mosquito vector during parasite development and transmission.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei148 – 1481Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. chitinase activity Source: UniProtKB-EC
  2. chitin binding Source: UniProtKB-KW

GO - Biological processi

  1. chitin catabolic process Source: UniProtKB-KW
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Keywords - Ligandi

Calcium, Chitin-binding

Protein family/group databases

CAZyiCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase (EC:3.2.1.14)
Alternative name(s):
MF1 antigen
OrganismiBrugia malayi (Filarial nematode worm)
Taxonomic identifieri6279 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaSpiruridaFilarioideaOnchocercidaeBrugia

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Add
BLAST
Chaini23 – 504482EndochitinasePRO_0000011947Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi480 ↔ 493PROSITE-ProRule annotation

Post-translational modificationi

O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

The appearance of the MF1 antigen correspond with the onset of the parasite's ability to infect the mosquito.

Structurei

3D structure databases

ProteinModelPortaliP29030.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati407 – 420141Add
BLAST
Repeati421 – 434142Add
BLAST
Repeati435 – 448143; approximateAdd
BLAST
Domaini448 – 50457Chitin-binding type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 400378CatalyticAdd
BLAST
Regioni407 – 448423 X 14 AA approximate tandem repeats of E-T-E-A-Y-[ED]-T-D-E-T-E-E-T-SAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi401 – 44848Ser/Thr-rich (linker)Add
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29030-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRTTLILFF IILSNTITVI HGYVRGCYYT NWAQYRDGEG KFLPGNIPNG
60 70 80 90 100
LCTHILYAFA KVDELGDSKP FEWNDEDTEW SKGMYSAVTK LRETNPGLKV
110 120 130 140 150
LLSYGGYNFG SAIFTGIAKS AQKTERFIKS AIAFLRKNNF DGFDLDWEYP
160 170 180 190 200
VGVAEEHAKL VEAMKTAFVE EAKTSGKQRL LLTAAVSAGK GTIDGSYNVE
210 220 230 240 250
SLGKNFDLLF LMSYDLHGSW EKNVDLHGKL HPTKGEVSGI GIFNTEFAAD
260 270 280 290 300
YWASKGMPKE KIIIGIPMYA QGWTLDNPSE TAIGAAASRP SSASKTNPAG
310 320 330 340 350
GTASYWEICK YLKEGGKETV HQEGVGAYMV KGDQWYGYDN EETIRIKMKW
360 370 380 390 400
LKEKGYGGAF IWALDFDDFT GKSCGKGPYP LLNAISSELE GESENPEITT
410 420 430 440 450
EEPSITETEA YETDETEETS ETEAYDTDET EETSETEATT YDTDETEGQE
460 470 480 490 500
CPERDGLFPH PTDCHLFIQC ANNIAYVMQC PATTFFNDAI KVCDHMTNAP

DTCI
Length:504
Mass (Da):55,971
Last modified:December 1, 1992 - v1
Checksum:iA78BE7BFB8E3709B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73689 mRNA. Translation: AAA27854.1.
PIRiA38221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73689 mRNA. Translation: AAA27854.1 .
PIRi A38221.

3D structure databases

ProteinModelPortali P29030.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view ]
SMARTi SM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEi PS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis."
    Fuhrman J.A., Lane W.S., Smith R.F., Piessens W.F., Perler F.B.
    Proc. Natl. Acad. Sci. U.S.A. 89:1548-1552(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiCHIT_BRUMA
AccessioniPrimary (citable) accession number: P29030
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 1, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Known to bind calcium.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3