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P29030

- CHIT_BRUMA

UniProt

P29030 - CHIT_BRUMA

Protein

Endochitinase

Gene
N/A
Organism
Brugia malayi (Filarial nematode worm)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Microfilarial chitinase, which may function to degrade chitin-containing structures in the micro-filaria or in its mosquito vector during parasite development and transmission.

    Catalytic activityi

    Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei148 – 1481Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. chitinase activity Source: UniProtKB-EC
    2. chitin binding Source: UniProtKB-KW

    GO - Biological processi

    1. chitin catabolic process Source: UniProtKB-KW
    2. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

    Keywords - Ligandi

    Calcium, Chitin-binding

    Protein family/group databases

    CAZyiCBM14. Carbohydrate-Binding Module Family 14.
    GH18. Glycoside Hydrolase Family 18.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endochitinase (EC:3.2.1.14)
    Alternative name(s):
    MF1 antigen
    OrganismiBrugia malayi (Filarial nematode worm)
    Taxonomic identifieri6279 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaSpiruridaFilarioideaOnchocercidaeBrugia

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Add
    BLAST
    Chaini23 – 504482EndochitinasePRO_0000011947Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi480 ↔ 493PROSITE-ProRule annotation

    Post-translational modificationi

    O-glycosylated.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Developmental stagei

    The appearance of the MF1 antigen correspond with the onset of the parasite's ability to infect the mosquito.

    Structurei

    3D structure databases

    ProteinModelPortaliP29030.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati407 – 420141Add
    BLAST
    Repeati421 – 434142Add
    BLAST
    Repeati435 – 448143; approximateAdd
    BLAST
    Domaini448 – 50457Chitin-binding type-2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni23 – 400378CatalyticAdd
    BLAST
    Regioni407 – 448423 X 14 AA approximate tandem repeats of E-T-E-A-Y-[ED]-T-D-E-T-E-E-T-SAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi401 – 44848Ser/Thr-rich (linker)Add
    BLAST

    Sequence similaritiesi

    Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.170.140.10. 1 hit.
    3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR002557. Chitin-bd_dom.
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01607. CBM_14. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view]
    SMARTiSM00494. ChtBD2. 1 hit.
    SM00636. Glyco_18. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    SSF57625. SSF57625. 1 hit.
    PROSITEiPS50940. CHIT_BIND_II. 1 hit.
    PS01095. CHITINASE_18. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29030-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRTTLILFF IILSNTITVI HGYVRGCYYT NWAQYRDGEG KFLPGNIPNG    50
    LCTHILYAFA KVDELGDSKP FEWNDEDTEW SKGMYSAVTK LRETNPGLKV 100
    LLSYGGYNFG SAIFTGIAKS AQKTERFIKS AIAFLRKNNF DGFDLDWEYP 150
    VGVAEEHAKL VEAMKTAFVE EAKTSGKQRL LLTAAVSAGK GTIDGSYNVE 200
    SLGKNFDLLF LMSYDLHGSW EKNVDLHGKL HPTKGEVSGI GIFNTEFAAD 250
    YWASKGMPKE KIIIGIPMYA QGWTLDNPSE TAIGAAASRP SSASKTNPAG 300
    GTASYWEICK YLKEGGKETV HQEGVGAYMV KGDQWYGYDN EETIRIKMKW 350
    LKEKGYGGAF IWALDFDDFT GKSCGKGPYP LLNAISSELE GESENPEITT 400
    EEPSITETEA YETDETEETS ETEAYDTDET EETSETEATT YDTDETEGQE 450
    CPERDGLFPH PTDCHLFIQC ANNIAYVMQC PATTFFNDAI KVCDHMTNAP 500
    DTCI 504
    Length:504
    Mass (Da):55,971
    Last modified:December 1, 1992 - v1
    Checksum:iA78BE7BFB8E3709B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73689 mRNA. Translation: AAA27854.1.
    PIRiA38221.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73689 mRNA. Translation: AAA27854.1 .
    PIRi A38221.

    3D structure databases

    ProteinModelPortali P29030.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM14. Carbohydrate-Binding Module Family 14.
    GH18. Glycoside Hydrolase Family 18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.170.140.10. 1 hit.
    3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR002557. Chitin-bd_dom.
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01607. CBM_14. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view ]
    SMARTi SM00494. ChtBD2. 1 hit.
    SM00636. Glyco_18. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    SSF57625. SSF57625. 1 hit.
    PROSITEi PS50940. CHIT_BIND_II. 1 hit.
    PS01095. CHITINASE_18. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis."
      Fuhrman J.A., Lane W.S., Smith R.F., Piessens W.F., Perler F.B.
      Proc. Natl. Acad. Sci. U.S.A. 89:1548-1552(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiCHIT_BRUMA
    AccessioniPrimary (citable) accession number: P29030
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Known to bind calcium.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3