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P29030 (CHIT_BRUMA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endochitinase

EC=3.2.1.14
Alternative name(s):
MF1 antigen
OrganismBrugia malayi (Filarial nematode worm)
Taxonomic identifier6279 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaSpiruridaFilarioideaOnchocercidaeBrugia

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Microfilarial chitinase, which may function to degrade chitin-containing structures in the micro-filaria or in its mosquito vector during parasite development and transmission.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Developmental stage

The appearance of the MF1 antigen correspond with the onset of the parasite's ability to infect the mosquito.

Post-translational modification

O-glycosylated.

Miscellaneous

Known to bind calcium.

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Contains 1 chitin-binding type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222
Chain23 – 504482Endochitinase
PRO_0000011947

Regions

Repeat407 – 420141
Repeat421 – 434142
Repeat435 – 448143; approximate
Domain448 – 50457Chitin-binding type-2
Region23 – 400378Catalytic
Region407 – 448423 X 14 AA approximate tandem repeats of E-T-E-A-Y-[ED]-T-D-E-T-E-E-T-S
Compositional bias401 – 44848Ser/Thr-rich (linker)

Sites

Active site1481Proton donor By similarity

Amino acid modifications

Disulfide bond480 ↔ 493 By similarity

Sequences

Sequence LengthMass (Da)Tools
P29030 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: A78BE7BFB8E3709B

FASTA50455,971
        10         20         30         40         50         60 
MNRTTLILFF IILSNTITVI HGYVRGCYYT NWAQYRDGEG KFLPGNIPNG LCTHILYAFA 

        70         80         90        100        110        120 
KVDELGDSKP FEWNDEDTEW SKGMYSAVTK LRETNPGLKV LLSYGGYNFG SAIFTGIAKS 

       130        140        150        160        170        180 
AQKTERFIKS AIAFLRKNNF DGFDLDWEYP VGVAEEHAKL VEAMKTAFVE EAKTSGKQRL 

       190        200        210        220        230        240 
LLTAAVSAGK GTIDGSYNVE SLGKNFDLLF LMSYDLHGSW EKNVDLHGKL HPTKGEVSGI 

       250        260        270        280        290        300 
GIFNTEFAAD YWASKGMPKE KIIIGIPMYA QGWTLDNPSE TAIGAAASRP SSASKTNPAG 

       310        320        330        340        350        360 
GTASYWEICK YLKEGGKETV HQEGVGAYMV KGDQWYGYDN EETIRIKMKW LKEKGYGGAF 

       370        380        390        400        410        420 
IWALDFDDFT GKSCGKGPYP LLNAISSELE GESENPEITT EEPSITETEA YETDETEETS 

       430        440        450        460        470        480 
ETEAYDTDET EETSETEATT YDTDETEGQE CPERDGLFPH PTDCHLFIQC ANNIAYVMQC 

       490        500 
PATTFFNDAI KVCDHMTNAP DTCI 

« Hide

References

[1]"Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis."
Fuhrman J.A., Lane W.S., Smith R.F., Piessens W.F., Perler F.B.
Proc. Natl. Acad. Sci. U.S.A. 89:1548-1552(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M73689 mRNA. Translation: AAA27854.1.
PIRA38221.

3D structure databases

ProteinModelPortalP29030.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.170.140.10. 1 hit.
3.20.20.80. 2 hits.
InterProIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
SSF57625. SSF57625. 1 hit.
PROSITEPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHIT_BRUMA
AccessionPrimary (citable) accession number: P29030
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 16, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries