Skip Header

 
Contribute Send feedback
Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot P29029 (CHIT_YEAST)

Last modified June 16, 2009. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Endochitinase
    EC=3.2.1.14
Alternative name(s):
    Soluble cell wall protein 2
Gene names
Name: CTS1
Synonyms: SCW2
Ordered Locus Names: YLR286C
ORF Names: L8003.13
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Chitinase is required for cell separation during growth of Saccharomyces cerevisiae.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Subcellular location

Secreted. Secretedcell wall. Note: Most of the enzyme is secreted, but a significant amount of chitinase is also found associated with the cell wall through binding of C-terminal domain to chitin. Ref.4

Post-translational modification

Extensively glycosylated with a series of short O-linked mannose oligosaccharides ranging in size from Man2 to Man5.

Miscellaneous

Present with 6350 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.4 Ref.1
Chain21 – 562542Endochitinase
PRO_0000011936

Regions

Region21 – 327307Catalytic
Region481 – 56282Chitin-binding, high affinity
Compositional bias328 – 480153Ser/Thr-rich

Sites

Active site1571Proton donor By similarity

Amino acid modifications

Glycosylation5531N-linked (GlcNAc...) Potential

Natural variations

Natural variant161L → P in strain: DBY939.
Natural variant231R → S in strain: DBY939 and SEY6210.
Natural variant3211T → S in strain: DBY939.
Natural variant336 – 3405Missing in strain: DBY939.
Natural variant3991S → A in strain: DBY939.
Natural variant433 – 4342PI → SL in strain: DBY939.
Natural variant4611T → K in strain: DBY939.
Natural variant477 – 4815Missing in strain: DBY939.

Experimental info

Sequence conflict1681A → R in AAA34539. Ref.1
Sequence conflict1681A → R in AAA34538. Ref.1

Secondary structure

................................................... 562
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29029-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 0ABCEFBF448E1E19

FASTA56259,015
        10         20         30         40         50         60 
MSLLYIILLF TQFLLLPTDA FDRSANTNIA VYWGQNSAGT QESLATYCES SDADIFLLSF 

        70         80         90        100        110        120 
LNQFPTLGLN FANACSDTFS DGLLHCTQIA EDIETCQSLG KKVLLSLGGA SGSYLFSDDS 

       130        140        150        160        170        180 
QAETFAQTLW DTFGEGTGAS ERPFDSAVVD GFDFDIENNN EVGYSALATK LRTLFAEGTK 

       190        200        210        220        230        240 
QYYLSAAPQC PYPDASVGDL LENADIDFAF IQFYNNYCSV SGQFNWDTWL TYAQTVSPNK 

       250        260        270        280        290        300 
NIKLFLGLPG SASAAGSGYI SDTSLLESTI ADIASSSSFG GIALWDASQA FSNELNGEPY 

       310        320        330        340        350        360 
VEILKNLLTS ASQTATTTVA TSKTSAASTS SASTSSASTS QKKTTQSTTS TQSKSKVTLS 

       370        380        390        400        410        420 
PTASSAIKTS ITQTTKTLTS STKTKSSLGT TTTESTLNSV AITSMKTTLS SQITSAALVT 

       430        440        450        460        470        480 
PQTTTTSIVS SAPIQTAITS TLSPATKSSS VVSLQTATTS TLSPTTTSTS SGSTSSGSTS 

       490        500        510        520        530        540 
SDSTARTLAK ELNAQYAAGK LNGKSTCTEG EIACSADGKF AVCDHSAWVY MECASGTTCY 

       550        560 
AYDSGDSVYT QCNFSYLESN YF 

« Hide

References

« Hide 'large scale' references
[1]"Chitinase is required for cell separation during growth of Saccharomyces cerevisiae."
Kuranda M.J., Robbins P.W.
J. Biol. Chem. 266:19758-19767(1991) [PubMed: 1918080] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 21-38.
Strain: DBY918 and DBY939.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed: 9169871] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating."
Cappellaro C., Mrsa V., Tanner W.
J. Bacteriol. 180:5030-5037(1998) [PubMed: 9748433] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-32, SUBCELLULAR LOCATION.
Strain: ATCC 96099 / S288c / SEY6210.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

M74070 Genomic DNA. Translation: AAA34539.1.
M74069 Genomic DNA. Translation: AAA34538.1.
U17243 Genomic DNA. Translation: AAB67331.1.
AY693040 Genomic DNA. Translation: AAT93059.1.
PIRA41035.
B41035.
S50371.
RefSeqNP_013388.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2UY2X-ray1.60A22-315[»]
2UY3X-ray1.90A22-315[»]
2UY4X-ray1.75A22-315[»]
2UY5X-ray1.60A22-315[»]
ModBaseSearch...

Protein family/group databases

CAZyCBM19. Carbohydrate-Binding Module Family 19.
GH18. Glycoside Hydrolase Family 18.

Proteomic databases

PeptideAtlasP29029.

Genome annotation databases

EnsemblYLR286C. Saccharomyces cerevisiae. [Contig view]
GeneID850992.
GenomeReviewsGene locus YLR286C in contig Y13138_GR.
KEGGsce:YLR286C.
NMPDRfig|4932.3.peg.4404.

Organism-specific databases

CYGDYLR286c.
SGDS000004276. CTS1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP29029.
OMAP29029. LNFANAC.

Enzyme and pathway databases

BRENDA3.2.1.14. 250.

Gene expression databases

ArrayExpressP29029.
GermOnlineYLR286C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001223. Glyco_hydro18cat.
IPR005089. Glyco_hydro_18_carb-bd.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF03427. CBM_19. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
PROSITEPS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio967519.

Entry information

Entry nameCHIT_YEAST
AccessionPrimary (citable) accession number: P29029
Secondary accession number(s): P29028
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1996
Last modified: June 16, 2009
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents