Reviewed, UniProtKB/Swiss-Prot P29026 (CHI1_RHIOL)

Names and origin

Protein names

Recommended name:
    Chitinase 1
    EC=3.2.1.14

Gene names

Name:

CHI1

Organism

Rhizopus oligosporus

Taxonomic identifier

4847 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Rhizopus

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably involved in the apical growth and branching of fungal hyphae.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subunit structure	Monomer.
Subcellular location	Secreted Probable.
Post-translational modification	O-glycosylated.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.1
	Chain	23 – 417	395	Chitinase 1		PRO_0000011930
	Propeptide	418 – 540	123	Potential		PRO_0000011931
Regions
	Region	23 – 312	290	Catalytic
	Region	353 – 404	52	Chitin-binding, high affinity
	Compositional bias	313 – 352	40	Ser/Thr-rich
Sites
	Active site	166	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29026-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: C7A8590D03881F1B	FASTA	540	56,227
	10 20 30 40 50 60 MLARTFLGMA ISAFLASTGV QAAWSSNGPN VMYYWGQNSA GGSNTQASLG TYCESGQVDA 70 80 90 100 110 120 VLLSFLHVFN VGGTPEINLS SACAGTYFPN TQLLSCPAVG ADIKKCQDKG VKVILSLGGA 130 140 150 160 170 180 AGVYGFTSDA QGQQFAQTIW NLFGGGSSDT RPFGDAVIDG VDLDIEGGAS TGYAAFVNAL 190 200 210 220 230 240 RQKFSSNFLI GAAPQCPFPD AILGSVLNSA SFDYVNVQFY NNYCSATGSS FNFDTWDNWA 250 260 270 280 290 300 KTTSPNKNVK IMFTIPGSPT AAGSGYVPMS TLQTIVPSLA SEYSSYGGVS VWDASQAWNN 310 320 330 340 350 360 GDFSSQLYSL VHSGGSTPPP SSSTIKTTTK ATTTSTKTTT TAAPTATSAP GSCPVANQSC 370 380 390 400 410 420 STQNQYACTA DGKYAVCDHG KWVVSSCPSG TVCIPTTDGT SIYCGYATGS GSTCPSASAL 430 440 450 460 470 480 EIAAASFGSK NGPVPRPYKA SKVAAQLAVT STDKNSFEAV INARRTTLTP FEKSVTIEFT 490 500 510 520 530 540 TPSNIKFTES DMGPVRQVGN KVRIQAKNDY NESMTLVVKV KGSINSGVFV APSTSAWKFK

References

[1]

"Purification of two chitinases from Rhizopus oligosporus and isolation and sequencing of the encoding genes." Yanai K., Takaya N., Kojima N., Horiuchi H., Ohta A., Takagi M. J. Bacteriol. 174:7398-7406(1992) [PubMed: 1429462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-52. Strain: ATCC 22959 / AS 3.4818 / CBS 338.62 / IFO 8631 / NRRL 2710.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10157 Genomic DNA. Translation: BAA01021.1.
PIR	A47022.
3D structure databases
HSSP	HSSP built from PDB template 2HVM based on UniProtKB P23472.
ModBase	Search...
Protein family/group databases
CAZy	CBM19. Carbohydrate-Binding Module Family 19. GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 81726.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR005089. Glyco_hydro_18_carb-bd. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF03427. CBM_19. 1 hit. PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI1_RHIOL

Accession

Primary (citable) accession number: P29026

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)