Reviewed, UniProtKB/Swiss-Prot P29024 (CHIA_PHAAN)

Names and origin

Protein names	Recommended name:     Acidic endochitinase     EC=3.2.1.14
Organism	Phaseolus angularis (Adzuki bean) (Vigna angularis)
Taxonomic identifier	3914 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein functions as a defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space.
Induction	By ethylene.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 298	269	Acidic endochitinase		PRO_0000011917
Sites
	Active site	156	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	49 ↔ 96		By similarity
	Disulfide bond	79 ↔ 86		By similarity
	Disulfide bond	185 ↔ 214		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29024-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 3B382DE1C07656A9	FASTA	298	31,702
	10 20 30 40 50 60 MKPNMACLKQ VSALLLPLLF ISFFKPSHAG GISVYWGQNG NEGSLADACN TGNYKYVNIA 70 80 90 100 110 120 FLFTFGGGQT PQLNLAGHCN PSINNCNVFS DQIKECQSKD IKVLLSLGGA SGSYSLTSAD 130 140 150 160 170 180 DATQVANYIW NNFLGGQSSS RPLGDAILDG VDFDIESGTG EHWDDLARAL KGFNSQLLLT 190 200 210 220 230 240 AAPQCPIPDA HLDTAIKTGL FDIVWVQFYN NPPCQYSSGN TNDLISSWNQ WTSSQAKQLF 250 260 270 280 290 LGVPASTAAA GSGFIPADVL TSQVLPTIKG SSKYGGVMLW DRFNDGQSGY SGAIIGSV

References

[1]	"Cloning of a complementary DNA that encodes an acidic chitinase which is induced by ethylene and expression of the corresponding gene." Ishige F., Mori H., Yamazaki K., Imaseki H. Plant Cell Physiol. 34:103-111(1993) [PubMed: 7517787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D11335 mRNA. Translation: BAA01948.1. Different initiation.
PIR	S36932.
3D structure databases
HSSP	HSSP built from PDB template 2HVM based on UniProtKB P23472.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 258509.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_PHAAN

Accession

Primary (citable) accession number: P29024

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	September 22, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)