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P29022 (CHIA_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endochitinase A

EC=3.2.1.14
Alternative name(s):
Seed chitinase A
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length280 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Defense against chitin containing fungal pathogens.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Miscellaneous

Maize chitinase B seems to be less active than chitinase A.

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Contains 1 chitin-binding type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 280255Endochitinase A
PRO_0000005303

Regions

Domain26 – 6035Chitin-binding type-1
Region61 – 7717Hinge region (poly-Gly)
Region78 – 280203Catalytic

Amino acid modifications

Disulfide bond28 ↔ 36 By similarity
Disulfide bond30 ↔ 42 By similarity
Disulfide bond35 ↔ 49 By similarity
Disulfide bond53 ↔ 58 By similarity
Disulfide bond100 ↔ 149 By similarity
Disulfide bond161 ↔ 170 By similarity
Disulfide bond248 ↔ 280 By similarity

Sequences

Sequence LengthMass (Da)Tools
P29022 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 4FC5BB7D938C1CC1

FASTA28029,125
        10         20         30         40         50         60 
MANAPRILAL GLLALLCAAA GPAAAQNCGC QPNFCCSKFG YCGTTDAYCG DGCQSGPCRS 

        70         80         90        100        110        120 
GGGGGGGGGG GGGGSGGANV ANVVTDAFFN GIKNQAGSGC EGKNFYTRSA FLSAVNAYPG 

       130        140        150        160        170        180 
FAHGGTEVEG KREIAAFFAH VTHETGHFCY ISEINKSNAY CDASNRQWPC AAGQKYYGRG 

       190        200        210        220        230        240 
PLQISWNYNY GPAGRDIGFN GLADPNRVAQ DAVIAFKTAL WFWMNNVHGV MPQGFGATIR 

       250        260        270        280 
AINGALECNG NNPAQMNARV GYYKQYCQQL RVDPGPNLIC 

« Hide

References

[1]"Antifungal proteins from plants. Purification, molecular cloning, and antifungal properties of chitinases from maize seed."
Huynh Q.K., Hironaka C.M., Levine E.B., Smith C.E., Borgmeyer J.R., Shah D.M.
J. Biol. Chem. 267:6635-6640(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Seed.
[2]"Identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from Zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide."
Verburg J.G., Smith C.E., Lisek C.A., Huynh Q.K.
J. Biol. Chem. 267:3886-3893(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 180-195.
Tissue: Seed.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84164 Genomic DNA. Translation: AAA33444.1.
PIRA42424.
UniGeneZm.119908.

3D structure databases

ProteinModelPortalP29022.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PRIDEP29022.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP29022.
MaizeGDB25130.

Family and domain databases

Gene3D3.30.60.10. 1 hit.
InterProIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
ProDomPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHIA_MAIZE
AccessionPrimary (citable) accession number: P29022
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: March 19, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries