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P29022

- CHIA_MAIZE

UniProt

P29022 - CHIA_MAIZE

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Protein
Endochitinase A
Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Defense against chitin containing fungal pathogens.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW
  2. chitinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response Source: UniProtKB-KW
  4. polysaccharide catabolic process Source: UniProtKB-KW
  5. somatic embryogenesis Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase A (EC:3.2.1.14)
Alternative name(s):
Seed chitinase A
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiP29022.
MaizeGDBi25130.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: EnsemblPlants/Gramene
  2. plant-type cell wall Source: EnsemblPlants/Gramene
  3. plasma membrane Source: EnsemblPlants/Gramene
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 280255Endochitinase A
PRO_0000005303Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 36 By similarity
Disulfide bondi30 ↔ 42 By similarity
Disulfide bondi35 ↔ 49 By similarity
Disulfide bondi53 ↔ 58 By similarity
Disulfide bondi100 ↔ 149 By similarity
Disulfide bondi161 ↔ 170 By similarity
Disulfide bondi248 ↔ 280 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP29022.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi86 – 938
Turni101 – 1055
Helixi108 – 1158
Turni119 – 1224
Helixi127 – 14519
Turni146 – 1494
Beta strandi164 – 1663
Turni180 – 1834
Helixi187 – 19711
Turni201 – 2033
Helixi207 – 2104
Helixi212 – 22615
Helixi228 – 2336
Helixi235 – 2428
Beta strandi246 – 2483
Helixi253 – 26917

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MCKX-ray1.50A86-278[»]
ProteinModelPortaliP29022.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 6035Chitin-binding type-1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni61 – 7717Hinge region (poly-Gly)
Add
BLAST
Regioni78 – 280203Catalytic
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29022-1 [UniParc]FASTAAdd to Basket

« Hide

MANAPRILAL GLLALLCAAA GPAAAQNCGC QPNFCCSKFG YCGTTDAYCG    50
DGCQSGPCRS GGGGGGGGGG GGGGSGGANV ANVVTDAFFN GIKNQAGSGC 100
EGKNFYTRSA FLSAVNAYPG FAHGGTEVEG KREIAAFFAH VTHETGHFCY 150
ISEINKSNAY CDASNRQWPC AAGQKYYGRG PLQISWNYNY GPAGRDIGFN 200
GLADPNRVAQ DAVIAFKTAL WFWMNNVHGV MPQGFGATIR AINGALECNG 250
NNPAQMNARV GYYKQYCQQL RVDPGPNLIC 280
Length:280
Mass (Da):29,125
Last modified:December 1, 1992 - v1
Checksum:i4FC5BB7D938C1CC1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84164 Genomic DNA. Translation: AAA33444.1.
PIRiA42424.
UniGeneiZm.119908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84164 Genomic DNA. Translation: AAA33444.1 .
PIRi A42424.
UniGenei Zm.119908.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4MCK X-ray 1.50 A 86-278 [» ]
ProteinModelPortali P29022.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PRIDEi P29022.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P29022.
MaizeGDBi 25130.

Family and domain databases

Gene3Di 3.30.60.10. 1 hit.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view ]
PIRSFi PIRSF001060. Endochitinase. 1 hit.
ProDomi PD000609. Chitin_bd_1. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Antifungal proteins from plants. Purification, molecular cloning, and antifungal properties of chitinases from maize seed."
    Huynh Q.K., Hironaka C.M., Levine E.B., Smith C.E., Borgmeyer J.R., Shah D.M.
    J. Biol. Chem. 267:6635-6640(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Seed.
  2. "Identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from Zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide."
    Verburg J.G., Smith C.E., Lisek C.A., Huynh Q.K.
    J. Biol. Chem. 267:3886-3893(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 180-195.
    Tissue: Seed.

Entry informationi

Entry nameiCHIA_MAIZE
AccessioniPrimary (citable) accession number: P29022
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Maize chitinase B seems to be less active than chitinase A.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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