Reviewed, UniProtKB/Swiss-Prot P29020 (CD3H_MOUSE)

Names and origin

Protein names

Recommended name:
    T-cell surface glycoprotein CD3 eta chain
Alternative name(s):
    T-cell receptor T3 eta chain

Gene names

Name:	Cd247
Synonyms:	Cd3z, Tcrz

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Subunit structure	The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-eta can be complexed in a heterodimeric form with CD3-zeta subunit. CD3-eta homodimer has not been observed.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the CD3Z/FCER1G family. Contains 2 ITAM domains.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane
Molecular function	Receptor
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	T cell receptor signaling pathway Inferred from direct assay. Source: MGI
Cellular component	alpha-beta T cell receptor complex Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: MGI transmembrane receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CD-3-eta (identifier: P29020-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CD-3-zeta (identifier: P24161-1)

The sequence of this isoform can be found in the external entry P24161-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 206	185	T-cell surface glycoprotein CD3 eta chain		PRO_0000016495
Regions
	Topological domain	22 – 30	9	Extracellular Potential
	Transmembrane	31 – 51	21	Potential
	Topological domain	52 – 206	155	Cytoplasmic Potential
	Domain	61 – 89	29	ITAM 1
	Domain	100 – 128	29	ITAM 2
Amino acid modifications
	Modified residue	22	1	Blocked amino end (Gln)
	Disulfide bond	32		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform CD-3-eta [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 829256A2CF44E444	FASTA	206	23,339
	10 20 30 40 50 60 MKWKVSVLAC ILHVRFPGAE AQSFGLLDPK LCYLLDGILF IYGVIITALY LRAKFSRSAE 70 80 90 100 110 120 TAANLQDPNQ LYNELNLGRR EEYDVLEKKR ARDPEMGGKQ QRRRNPQEGV YNALQKDKMA 130 140 150 160 170 180 EAYSEIGTKG ERRRGKGHDG LYQDSHFQAV QFGNRREREG SELTRTLGLR ARPKGESTQQ 190 200 SSQSCASVFS IPTLWSPWPP SSSSQL
	Isoform CD-3-zeta. See P24161.	FASTA	–	–

References

[1]	"Molecular cloning of the CD3 eta subunit identifies a CD3 zeta-related product in thymus-derived cells." Jin Y.J., Clayton L.K., Howard F.D., Koyasu S., Sieh M., Steinbrich R., Tarr G.E., Reinherz E.L. Proc. Natl. Acad. Sci. U.S.A. 87:3319-3323(1990) [PubMed: 2139725] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, BLOCKAGE OF N-TERMINUS.
[2]	"CD3 eta and CD3 zeta are alternatively spliced products of a common genetic locus and are transcriptionally and/or post-transcriptionally regulated during T-cell development." Clayton L.K., D'Adamio L., Sieh M., Hussey R.E., Koyasu S., Reinherz E.L., Howard F.B. Proc. Natl. Acad. Sci. U.S.A. 88:5202-5206(1991) [PubMed: 1828894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 144-206.
[3]	"CD3 zeta and eta chains are produced by alternative splicing from a common gene." Ohno H., Saito T. Int. Immunol. 2:1117-1119(1990) [PubMed: 2150596] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 144-206.
[4]	Erratum Ohno H., Saito T. Int. Immunol. 4:1339-1339(1992)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33158 mRNA. Translation: AAA37398.1. M76711 Genomic DNA. Translation: AAA40403.1.
IPI	IPI00120468.
PIR	A35900.
RefSeq	NP_001106864.1. NP_001106865.1. NP_112439.1.
UniGene	Mm.217308 Mm.475205
3D structure databases
SMR	P29020. Positions 28-60.
ModBase	Search...
Protein-protein interaction databases
STRING	P29020.
Proteomic databases
PRIDE	P29020.
Genome annotation databases
Ensembl	ENSMUST00000027849; ENSMUSP00000027849; ENSMUSG00000005763; Mus musculus. [Genome view]
GeneID	12503.
UCSC	uc007djo.1. mouse.
Organism-specific databases
CTD	12503.
MGI	MGI:88334. Cd247.
Phylogenomic databases
HOGENOM	HBG278836.
HOVERGEN	P29020.
InParanoid	P29020.
Gene expression databases
ArrayExpress	P29020.
Bgee	P29020.
CleanEx	MM_CD247.
Genevestigator	P29020.
GermOnline	ENSMUSG00000005763. Mus musculus.
Family and domain databases
InterPro	IPR003110. Phos_immunorcpt_sig_ITAM. [Graphical view]
Pfam	PF02189. ITAM. 2 hits. [Graphical view]
SMART	SM00077. ITAM. 2 hits. [Graphical view]
PROSITE	PS51055. ITAM_1. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	281458.
SOURCE	Search...

Entry information

Entry name

CD3H_MOUSE

Accession

Primary (citable) accession number: P29020

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 19, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)