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Protein

T-cell surface glycoprotein CD3 eta chain

Gene

Cd247

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

GO - Molecular functioni

GO - Biological processi

  • T cell receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiR-MMU-202424. Downstream TCR signaling.
R-MMU-202427. Phosphorylation of CD3 and TCR zeta chains.
R-MMU-202430. Translocation of ZAP-70 to Immunological synapse.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-2029485. Role of phospholipids in phagocytosis.
R-MMU-389948. PD-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 eta chain
Alternative name(s):
T-cell receptor T3 eta chain
Gene namesi
Name:Cd247
Synonyms:Cd3z, Tcrz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88334. Cd247.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 309ExtracellularSequence analysis
Transmembranei31 – 5121HelicalSequence analysisAdd
BLAST
Topological domaini52 – 206155CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 206185T-cell surface glycoprotein CD3 eta chainPRO_0000016495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Blocked amino end (Gln)
Disulfide bondi32 – 32InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP29020.
MaxQBiP29020.
PaxDbiP29020.
PRIDEiP29020.

PTM databases

SwissPalmiP29020.

Expressioni

Gene expression databases

BgeeiP29020.
CleanExiMM_CD247.
ExpressionAtlasiP29020. baseline and differential.
GenevisibleiP29020. MM.

Interactioni

Subunit structurei

The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-eta can be complexed in a heterodimeric form with CD3-zeta subunit. CD3-eta homodimer has not been observed.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198598. 5 interactions.
STRINGi10090.ENSMUSP00000027849.

Structurei

3D structure databases

ProteinModelPortaliP29020.
SMRiP29020. Positions 28-60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 8929ITAM 1PROSITE-ProRule annotationAdd
BLAST
Domaini100 – 12829ITAM 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CD3Z/FCER1G family.Curated
Contains 2 ITAM domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J0ID. Eukaryota.
ENOG411241T. LUCA.
GeneTreeiENSGT00390000018208.
HOGENOMiHOG000234398.
HOVERGENiHBG005280.
InParanoidiP29020.
PhylomeDBiP29020.

Family and domain databases

InterProiIPR021663. CR3_zeta/IgE_Fc_rcpt_gamma.
IPR003110. Phos_immunorcpt_sig_ITAM.
IPR024128. T-cell_CD3_zeta/eta.
[Graphical view]
PANTHERiPTHR10035. PTHR10035. 1 hit.
PfamiPF02189. ITAM. 2 hits.
PF11628. TCR_zetazeta. 1 hit.
[Graphical view]
SMARTiSM00077. ITAM. 2 hits.
[Graphical view]
PROSITEiPS51055. ITAM_1. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform CD-3-eta (identifier: P29020-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKWKVSVLAC ILHVRFPGAE AQSFGLLDPK LCYLLDGILF IYGVIITALY
60 70 80 90 100
LRAKFSRSAE TAANLQDPNQ LYNELNLGRR EEYDVLEKKR ARDPEMGGKQ
110 120 130 140 150
QRRRNPQEGV YNALQKDKMA EAYSEIGTKG ERRRGKGHDG LYQDSHFQAV
160 170 180 190 200
QFGNRREREG SELTRTLGLR ARPKGESTQQ SSQSCASVFS IPTLWSPWPP

SSSSQL
Length:206
Mass (Da):23,339
Last modified:December 1, 1992 - v1
Checksum:i829256A2CF44E444
GO
Isoform CD-3-zeta (identifier: P24161-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P24161.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:164
Mass (Da):18,637
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33158 mRNA. Translation: AAA37398.1.
M76711 Genomic DNA. Translation: AAA40403.1.
CCDSiCCDS15443.1. [P29020-1]
PIRiA35900.
RefSeqiNP_112439.1. NM_031162.4. [P29020-1]
UniGeneiMm.217308.
Mm.245261.
Mm.475205.

Genome annotation databases

EnsembliENSMUST00000027849; ENSMUSP00000027849; ENSMUSG00000005763. [P29020-1]
ENSMUST00000086002; ENSMUSP00000083165; ENSMUSG00000005763. [P29020-1]
ENSMUST00000187313; ENSMUSP00000140926; ENSMUSG00000005763. [P29020-1]
GeneIDi12503.
UCSCiuc007djo.2. mouse. [P29020-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33158 mRNA. Translation: AAA37398.1.
M76711 Genomic DNA. Translation: AAA40403.1.
CCDSiCCDS15443.1. [P29020-1]
PIRiA35900.
RefSeqiNP_112439.1. NM_031162.4. [P29020-1]
UniGeneiMm.217308.
Mm.245261.
Mm.475205.

3D structure databases

ProteinModelPortaliP29020.
SMRiP29020. Positions 28-60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198598. 5 interactions.
STRINGi10090.ENSMUSP00000027849.

PTM databases

SwissPalmiP29020.

Proteomic databases

EPDiP29020.
MaxQBiP29020.
PaxDbiP29020.
PRIDEiP29020.

Protocols and materials databases

DNASUi12503.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027849; ENSMUSP00000027849; ENSMUSG00000005763. [P29020-1]
ENSMUST00000086002; ENSMUSP00000083165; ENSMUSG00000005763. [P29020-1]
ENSMUST00000187313; ENSMUSP00000140926; ENSMUSG00000005763. [P29020-1]
GeneIDi12503.
UCSCiuc007djo.2. mouse. [P29020-1]

Organism-specific databases

CTDi919.
MGIiMGI:88334. Cd247.

Phylogenomic databases

eggNOGiENOG410J0ID. Eukaryota.
ENOG411241T. LUCA.
GeneTreeiENSGT00390000018208.
HOGENOMiHOG000234398.
HOVERGENiHBG005280.
InParanoidiP29020.
PhylomeDBiP29020.

Enzyme and pathway databases

ReactomeiR-MMU-202424. Downstream TCR signaling.
R-MMU-202427. Phosphorylation of CD3 and TCR zeta chains.
R-MMU-202430. Translocation of ZAP-70 to Immunological synapse.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-2029485. Role of phospholipids in phagocytosis.
R-MMU-389948. PD-1 signaling.

Miscellaneous databases

ChiTaRSiCd247. mouse.
NextBioi281458.
SOURCEiSearch...

Gene expression databases

BgeeiP29020.
CleanExiMM_CD247.
ExpressionAtlasiP29020. baseline and differential.
GenevisibleiP29020. MM.

Family and domain databases

InterProiIPR021663. CR3_zeta/IgE_Fc_rcpt_gamma.
IPR003110. Phos_immunorcpt_sig_ITAM.
IPR024128. T-cell_CD3_zeta/eta.
[Graphical view]
PANTHERiPTHR10035. PTHR10035. 1 hit.
PfamiPF02189. ITAM. 2 hits.
PF11628. TCR_zetazeta. 1 hit.
[Graphical view]
SMARTiSM00077. ITAM. 2 hits.
[Graphical view]
PROSITEiPS51055. ITAM_1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the CD3 eta subunit identifies a CD3 zeta-related product in thymus-derived cells."
    Jin Y.J., Clayton L.K., Howard F.D., Koyasu S., Sieh M., Steinbrich R., Tarr G.E., Reinherz E.L.
    Proc. Natl. Acad. Sci. U.S.A. 87:3319-3323(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, BLOCKAGE OF N-TERMINUS.
  2. "CD3 eta and CD3 zeta are alternatively spliced products of a common genetic locus and are transcriptionally and/or post-transcriptionally regulated during T-cell development."
    Clayton L.K., D'Adamio L., Sieh M., Hussey R.E., Koyasu S., Reinherz E.L., Howard F.B.
    Proc. Natl. Acad. Sci. U.S.A. 88:5202-5206(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 144-206.
  3. "CD3 zeta and eta chains are produced by alternative splicing from a common gene."
    Ohno H., Saito T.
    Int. Immunol. 2:1117-1119(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 144-206.
  4. Erratum
    Ohno H., Saito T.
    Int. Immunol. 4:1339-1339(1992)
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiCD3H_MOUSE
AccessioniPrimary (citable) accession number: P29020
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: March 16, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.