SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P29019

- GUN_BACSZ

UniProt

P29019 - GUN_BACSZ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Endoglucanase
Gene
N/A
Organism
Bacillus sp. (strain KSM-330)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.2. Active from pH 4.5 to 6.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei130 – 1301Proton donor By similarity
Active sitei191 – 1911Nucleophile Reviewed prediction

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Endo-K
OrganismiBacillus sp. (strain KSM-330)
Taxonomic identifieri72575 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction
Add
BLAST
Propeptidei28 – 5528 Reviewed prediction
PRO_0000007931Add
BLAST
Chaini56 – 463408Endoglucanase
PRO_0000007932Add
BLAST

Post-translational modificationi

The N- and the C-terminus may be subjected to proteolysis.

Structurei

3D structure databases

ProteinModelPortaliP29019.
SMRiP29019. Positions 57-442.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29019-1 [UniParc]FASTAAdd to Basket

« Hide

MVEKRKIFTV LCACGIGFTS YTSCISAAAI DNDTLINNGH KINSSIITNS    50
SQVSAVAKEM KPFPQQVNYS GILKPNHVSQ ESLNNAVKNY YNDWKKKYLK 100
NDLSSLPGGY YVKGEITGNP DGFRPLGTSE GQGYGMIITV LMAGHDSNAQ 150
TIYDGLFKTA RAFKSSINPN LMGWVVADDK KAQGHFDSAT DGDLDIAYSL 200
LLAHKQWGSS GKINYLKEAQ NMITKGIKAS NVTKNNGLNL GDWGDKSTFD 250
TRPSDWMMSH LRAFYEFTGD KTWLNVIDNL YNTYTNFTNK YSPKTGLISD 300
FVVKNPPQPA PKDFLDESKY TDSYYYNASR VPLRIVMDYA MYGEKRGKVI 350
SDKVATWIKS KTKGNPSKIV DGYKLDGTNI GDYPTAVYVS PFIAAGTTNS 400
KNQEWVNSGW DWMKNKKESY FSDSYNLLTM LFLTGNWWKP IPDEKKIQSP 450
INLEVQSELK EQD 463
Length:463
Mass (Da):51,883
Last modified:December 1, 1992 - v1
Checksum:i407FA54F5236C59E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M68872 Genomic DNA. Translation: AAA22409.1.
PIRiA44808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M68872 Genomic DNA. Translation: AAA22409.1 .
PIRi A44808.

3D structure databases

ProteinModelPortali P29019.
SMRi P29019. Positions 57-442.
ModBasei Search...

Protein family/group databases

CAZyi GH8. Glycoside Hydrolase Family 8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view ]
Pfami PF01270. Glyco_hydro_8. 1 hit.
[Graphical view ]
PRINTSi PR00735. GLHYDRLASE8.
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of the gene encoding an endo-1,4-beta-glucanase from Bacillus sp. KSM-330."
    Ozaki K., Sumitomo N., Ito S.
    J. Gen. Microbiol. 137:2299-2305(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Purification and properties of an acid endo-1,4-beta-glucanase from Bacillus sp. KSM-330."
    Ozaki K., Ito S.
    J. Gen. Microbiol. 137:41-48(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 56-75, CHARACTERIZATION.

Entry informationi

Entry nameiGUN_BACSZ
AccessioniPrimary (citable) accession number: P29019
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 16, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

One Trp residue has been proved to be involved in the mechanism of action of endo-K.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi