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P29019

- GUN_BACSZ

UniProt

P29019 - GUN_BACSZ

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Protein

Endoglucanase

Gene
N/A
Organism
Bacillus sp. (strain KSM-330)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.2. Active from pH 4.5 to 6.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei130 – 1301Proton donorBy similarity
Active sitei191 – 1911NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Endo-K
OrganismiBacillus sp. (strain KSM-330)
Taxonomic identifieri72575 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 5528Sequence AnalysisPRO_0000007931Add
BLAST
Chaini56 – 463408EndoglucanasePRO_0000007932Add
BLAST

Post-translational modificationi

The N- and the C-terminus may be subjected to proteolysis.

Structurei

3D structure databases

ProteinModelPortaliP29019.
SMRiP29019. Positions 57-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29019-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVEKRKIFTV LCACGIGFTS YTSCISAAAI DNDTLINNGH KINSSIITNS
60 70 80 90 100
SQVSAVAKEM KPFPQQVNYS GILKPNHVSQ ESLNNAVKNY YNDWKKKYLK
110 120 130 140 150
NDLSSLPGGY YVKGEITGNP DGFRPLGTSE GQGYGMIITV LMAGHDSNAQ
160 170 180 190 200
TIYDGLFKTA RAFKSSINPN LMGWVVADDK KAQGHFDSAT DGDLDIAYSL
210 220 230 240 250
LLAHKQWGSS GKINYLKEAQ NMITKGIKAS NVTKNNGLNL GDWGDKSTFD
260 270 280 290 300
TRPSDWMMSH LRAFYEFTGD KTWLNVIDNL YNTYTNFTNK YSPKTGLISD
310 320 330 340 350
FVVKNPPQPA PKDFLDESKY TDSYYYNASR VPLRIVMDYA MYGEKRGKVI
360 370 380 390 400
SDKVATWIKS KTKGNPSKIV DGYKLDGTNI GDYPTAVYVS PFIAAGTTNS
410 420 430 440 450
KNQEWVNSGW DWMKNKKESY FSDSYNLLTM LFLTGNWWKP IPDEKKIQSP
460
INLEVQSELK EQD
Length:463
Mass (Da):51,883
Last modified:December 1, 1992 - v1
Checksum:i407FA54F5236C59E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M68872 Genomic DNA. Translation: AAA22409.1.
PIRiA44808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M68872 Genomic DNA. Translation: AAA22409.1 .
PIRi A44808.

3D structure databases

ProteinModelPortali P29019.
SMRi P29019. Positions 57-442.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH8. Glycoside Hydrolase Family 8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view ]
Pfami PF01270. Glyco_hydro_8. 1 hit.
[Graphical view ]
PRINTSi PR00735. GLHYDRLASE8.
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of the gene encoding an endo-1,4-beta-glucanase from Bacillus sp. KSM-330."
    Ozaki K., Sumitomo N., Ito S.
    J. Gen. Microbiol. 137:2299-2305(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Purification and properties of an acid endo-1,4-beta-glucanase from Bacillus sp. KSM-330."
    Ozaki K., Ito S.
    J. Gen. Microbiol. 137:41-48(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 56-75, CHARACTERIZATION.

Entry informationi

Entry nameiGUN_BACSZ
AccessioniPrimary (citable) accession number: P29019
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 1, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

One Trp residue has been proved to be involved in the mechanism of action of endo-K.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3