P29019 (GUN_BACSZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoglucanase EC=3.2.1.4 Alternative name(s): Cellulase Endo-1,4-beta-glucanase Endo-K
Organism	Bacillus sp. (strain KSM-330)
Taxonomic identifier	72575 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Post-translational modification	The N- and the C-terminus may be subjected to proteolysis.
Miscellaneous	One Trp residue has been proved to be involved in the mechanism of action of endo-K.
Sequence similarities	Belongs to the glycosyl hydrolase 8 (cellulase D) family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.2. Active from pH 4.5 to 6.5.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 55	28	Potential		PRO_0000007931
	Chain	56 – 463	408	Endoglucanase		PRO_0000007932
Sites
	Active site	130	1	Proton donor By similarity
	Active site	191	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	P29019 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 407FA54F5236C59E	FASTA	463	51,883
	10 20 30 40 50 60 MVEKRKIFTV LCACGIGFTS YTSCISAAAI DNDTLINNGH KINSSIITNS SQVSAVAKEM 70 80 90 100 110 120 KPFPQQVNYS GILKPNHVSQ ESLNNAVKNY YNDWKKKYLK NDLSSLPGGY YVKGEITGNP 130 140 150 160 170 180 DGFRPLGTSE GQGYGMIITV LMAGHDSNAQ TIYDGLFKTA RAFKSSINPN LMGWVVADDK 190 200 210 220 230 240 KAQGHFDSAT DGDLDIAYSL LLAHKQWGSS GKINYLKEAQ NMITKGIKAS NVTKNNGLNL 250 260 270 280 290 300 GDWGDKSTFD TRPSDWMMSH LRAFYEFTGD KTWLNVIDNL YNTYTNFTNK YSPKTGLISD 310 320 330 340 350 360 FVVKNPPQPA PKDFLDESKY TDSYYYNASR VPLRIVMDYA MYGEKRGKVI SDKVATWIKS 370 380 390 400 410 420 KTKGNPSKIV DGYKLDGTNI GDYPTAVYVS PFIAAGTTNS KNQEWVNSGW DWMKNKKESY 430 440 450 460 FSDSYNLLTM LFLTGNWWKP IPDEKKIQSP INLEVQSELK EQD

References

[1]	"Molecular cloning and nucleotide sequence of the gene encoding an endo-1,4-beta-glucanase from Bacillus sp. KSM-330." Ozaki K., Sumitomo N., Ito S. J. Gen. Microbiol. 137:2299-2305(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Purification and properties of an acid endo-1,4-beta-glucanase from Bacillus sp. KSM-330." Ozaki K., Ito S. J. Gen. Microbiol. 137:41-48(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 56-75, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M68872 Genomic DNA. Translation: AAA22409.1.
PIR	A44808.
3D structure databases
ProteinModelPortal	P29019.
SMR	P29019. Positions 57-442.
ModBase	Search...
Protein family/group databases
CAZy	GH8. Glycoside Hydrolase Family 8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR002037. Glyco_hydro_8. IPR019834. Glyco_hydro_8_CS. [Graphical view]
Pfam	PF01270. Glyco_hydro_8. 1 hit. [Graphical view]
PRINTS	PR00735. GLHYDRLASE8.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00812. GLYCOSYL_HYDROL_F8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_BACSZ

Accession

Primary (citable) accession number: P29019

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program