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Protein

Endoglucanase

Gene
N/A
Organism
Bacillus sp. (strain KSM-330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.2. Active from pH 4.5 to 6.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei130Proton donorBy similarity1
Active sitei191NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

SABIO-RKP29019.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Endo-K
OrganismiBacillus sp. (strain KSM-330)
Taxonomic identifieri72575 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000000793128 – 55Sequence analysisAdd BLAST28
ChainiPRO_000000793256 – 463EndoglucanaseAdd BLAST408

Post-translational modificationi

The N- and the C-terminus may be subjected to proteolysis.

Structurei

3D structure databases

ProteinModelPortaliP29019.
SMRiP29019.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEKRKIFTV LCACGIGFTS YTSCISAAAI DNDTLINNGH KINSSIITNS
60 70 80 90 100
SQVSAVAKEM KPFPQQVNYS GILKPNHVSQ ESLNNAVKNY YNDWKKKYLK
110 120 130 140 150
NDLSSLPGGY YVKGEITGNP DGFRPLGTSE GQGYGMIITV LMAGHDSNAQ
160 170 180 190 200
TIYDGLFKTA RAFKSSINPN LMGWVVADDK KAQGHFDSAT DGDLDIAYSL
210 220 230 240 250
LLAHKQWGSS GKINYLKEAQ NMITKGIKAS NVTKNNGLNL GDWGDKSTFD
260 270 280 290 300
TRPSDWMMSH LRAFYEFTGD KTWLNVIDNL YNTYTNFTNK YSPKTGLISD
310 320 330 340 350
FVVKNPPQPA PKDFLDESKY TDSYYYNASR VPLRIVMDYA MYGEKRGKVI
360 370 380 390 400
SDKVATWIKS KTKGNPSKIV DGYKLDGTNI GDYPTAVYVS PFIAAGTTNS
410 420 430 440 450
KNQEWVNSGW DWMKNKKESY FSDSYNLLTM LFLTGNWWKP IPDEKKIQSP
460
INLEVQSELK EQD
Length:463
Mass (Da):51,883
Last modified:December 1, 1992 - v1
Checksum:i407FA54F5236C59E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68872 Genomic DNA. Translation: AAA22409.1.
PIRiA44808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68872 Genomic DNA. Translation: AAA22409.1.
PIRiA44808.

3D structure databases

ProteinModelPortaliP29019.
SMRiP29019.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP29019.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUN_BACSZ
AccessioniPrimary (citable) accession number: P29019
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

One Trp residue has been proved to be involved in the mechanism of action of endo-K.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.