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P29019

- GUN_BACSZ

UniProt

P29019 - GUN_BACSZ

Protein

Endoglucanase

Gene
N/A
Organism
Bacillus sp. (strain KSM-330)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    pH dependencei

    Optimum pH is 5.2. Active from pH 4.5 to 6.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei130 – 1301Proton donorBy similarity
    Active sitei191 – 1911NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH8. Glycoside Hydrolase Family 8.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase (EC:3.2.1.4)
    Alternative name(s):
    Cellulase
    Endo-1,4-beta-glucanase
    Endo-K
    OrganismiBacillus sp. (strain KSM-330)
    Taxonomic identifieri72575 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Propeptidei28 – 5528Sequence AnalysisPRO_0000007931Add
    BLAST
    Chaini56 – 463408EndoglucanasePRO_0000007932Add
    BLAST

    Post-translational modificationi

    The N- and the C-terminus may be subjected to proteolysis.

    Structurei

    3D structure databases

    ProteinModelPortaliP29019.
    SMRiP29019. Positions 57-442.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR002037. Glyco_hydro_8.
    IPR019834. Glyco_hydro_8_CS.
    [Graphical view]
    PfamiPF01270. Glyco_hydro_8. 1 hit.
    [Graphical view]
    PRINTSiPR00735. GLHYDRLASE8.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00812. GLYCOSYL_HYDROL_F8. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29019-1 [UniParc]FASTAAdd to Basket

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    MVEKRKIFTV LCACGIGFTS YTSCISAAAI DNDTLINNGH KINSSIITNS    50
    SQVSAVAKEM KPFPQQVNYS GILKPNHVSQ ESLNNAVKNY YNDWKKKYLK 100
    NDLSSLPGGY YVKGEITGNP DGFRPLGTSE GQGYGMIITV LMAGHDSNAQ 150
    TIYDGLFKTA RAFKSSINPN LMGWVVADDK KAQGHFDSAT DGDLDIAYSL 200
    LLAHKQWGSS GKINYLKEAQ NMITKGIKAS NVTKNNGLNL GDWGDKSTFD 250
    TRPSDWMMSH LRAFYEFTGD KTWLNVIDNL YNTYTNFTNK YSPKTGLISD 300
    FVVKNPPQPA PKDFLDESKY TDSYYYNASR VPLRIVMDYA MYGEKRGKVI 350
    SDKVATWIKS KTKGNPSKIV DGYKLDGTNI GDYPTAVYVS PFIAAGTTNS 400
    KNQEWVNSGW DWMKNKKESY FSDSYNLLTM LFLTGNWWKP IPDEKKIQSP 450
    INLEVQSELK EQD 463
    Length:463
    Mass (Da):51,883
    Last modified:December 1, 1992 - v1
    Checksum:i407FA54F5236C59E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68872 Genomic DNA. Translation: AAA22409.1.
    PIRiA44808.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68872 Genomic DNA. Translation: AAA22409.1 .
    PIRi A44808.

    3D structure databases

    ProteinModelPortali P29019.
    SMRi P29019. Positions 57-442.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH8. Glycoside Hydrolase Family 8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR002037. Glyco_hydro_8.
    IPR019834. Glyco_hydro_8_CS.
    [Graphical view ]
    Pfami PF01270. Glyco_hydro_8. 1 hit.
    [Graphical view ]
    PRINTSi PR00735. GLHYDRLASE8.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00812. GLYCOSYL_HYDROL_F8. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of the gene encoding an endo-1,4-beta-glucanase from Bacillus sp. KSM-330."
      Ozaki K., Sumitomo N., Ito S.
      J. Gen. Microbiol. 137:2299-2305(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Purification and properties of an acid endo-1,4-beta-glucanase from Bacillus sp. KSM-330."
      Ozaki K., Ito S.
      J. Gen. Microbiol. 137:41-48(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 56-75, CHARACTERIZATION.

    Entry informationi

    Entry nameiGUN_BACSZ
    AccessioniPrimary (citable) accession number: P29019
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    One Trp residue has been proved to be involved in the mechanism of action of endo-K.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3