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Protein

Alanine racemase, catabolic

Gene

dadX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA.By similarity

Catalytic activityi

L-alanine = D-alanine.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei35Proton acceptor; specific for D-alanineBy similarity1
Binding sitei130SubstrateBy similarity1
Active sitei253Proton acceptor; specific for L-alanineBy similarity1
Binding sitei301Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:ALARACECAT-MONOMER
MetaCyc:ALARACECAT-MONOMER
SABIO-RKiP29012

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, catabolic (EC:5.1.1.1)
Gene namesi
Name:dadX
Synonyms:alnB, dadB
Ordered Locus Names:b1190, JW1179
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11408 dadX

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001145171 – 356Alanine racemase, catabolicAdd BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

EPDiP29012
PaxDbiP29012
PRIDEiP29012

Expressioni

Inductioni

By alanine.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260105, 648 interactors
DIPiDIP-9395N
IntActiP29012, 2 interactors
STRINGi316385.ECDH10B_1243

Structurei

3D structure databases

ProteinModelPortaliP29012
SMRiP29012
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJ4 Bacteria
COG0787 LUCA
HOGENOMiHOG000031446
InParanoidiP29012
KOiK01775
OMAiWRGPILL
PhylomeDBiP29012

Family and domain databases

Gene3Di2.40.37.10, 1 hit
3.20.20.10, 1 hit
HAMAPiMF_01201 Ala_racemase, 1 hit
InterProiView protein in InterPro
IPR000821 Ala_racemase
IPR009006 Ala_racemase/Decarboxylase_C
IPR011079 Ala_racemase_C
IPR001608 Ala_racemase_N
IPR020622 Ala_racemase_pyridoxalP-BS
IPR029066 PLP-binding_barrel
PfamiView protein in Pfam
PF00842 Ala_racemase_C, 1 hit
PF01168 Ala_racemase_N, 1 hit
PRINTSiPR00992 ALARACEMASE
SMARTiView protein in SMART
SM01005 Ala_racemase_C, 1 hit
SUPFAMiSSF50621 SSF50621, 1 hit
SSF51419 SSF51419, 1 hit
TIGRFAMsiTIGR00492 alr, 1 hit
PROSITEiView protein in PROSITE
PS00395 ALANINE_RACEMASE, 1 hit

Sequencei

Sequence statusi: Complete.

P29012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRPIQASLD LQALKQNLSI VRQAATHARV WSVVKANAYG HGIERIWSAI
60 70 80 90 100
GATDGFALLN LEEAITLRER GWKGPILMLE GFFHAQDLEI YDQHRLTTCV
110 120 130 140 150
HSNWQLKALQ NARLKAPLDI YLKVNSGMNR LGFQPDRVLT VWQQLRAMAN
160 170 180 190 200
VGEMTLMSHF AEAEHPDGIS GAMARIEQAA EGLECRRSLS NSAATLWHPE
210 220 230 240 250
AHFDWVRPGI ILYGASPSGQ WRDIANTGLR PVMTLSSEII GVQTLKAGER
260 270 280 290 300
VGYGGRYTAR DEQRIGIVAA GYADGYPRHA PTGTPVLVDG VRTMTVGTVS
310 320 330 340 350
MDMLAVDLTP CPQAGIGTPV ELWGKEIKID DVAAAAGTVG YELMCALALR

VPVVTV
Length:356
Mass (Da):38,845
Last modified:November 1, 1997 - v2
Checksum:iFFF3226B47E5AAB3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172A → R in AAC36881 (PubMed:7906689).Curated1
Sequence conflicti215A → R in AAC36881 (PubMed:7906689).Curated1
Sequence conflicti281P → L in AAC36881 (PubMed:7906689).Curated1
Sequence conflicti349L → V in AAC36881 (PubMed:7906689).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02948 Unassigned DNA Translation: AAC36881.1
U00096 Genomic DNA Translation: AAC74274.1
AP009048 Genomic DNA Translation: BAA36045.1
PIRiC64865 C53383
RefSeqiNP_415708.1, NC_000913.3
WP_000197881.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74274; AAC74274; b1190
BAA36045; BAA36045; BAA36045
GeneIDi945754
KEGGiecj:JW1179
eco:b1190
PATRICifig|1411691.4.peg.1097

Similar proteinsi

Entry informationi

Entry nameiALR2_ECOLI
AccessioniPrimary (citable) accession number: P29012
Secondary accession number(s): O87498, P78246
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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