Reviewed, UniProtKB/Swiss-Prot P29006 (BMNL1_BOMVA)

Names and origin

Protein names	Recommended name:     Bombinin-like peptides 1 Cleaved into the following 5 chains:     1- Recommended name:             Acidic peptide 1-1     2- Recommended name:             Bombinin-like peptide 1                 Short name=BLP-1     3- Recommended name:             Octapeptide 1     4- Recommended name:             Acidic peptide 1-2     5- Recommended name:             Bombinin-H
Organism	Bombina variegata (Yellow-bellied toad)
Taxonomic identifier	8348 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Archeobatrachia › Bombinatoridae › Bombina

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has antimicrobial activity, but no hemolytic activity. Preliminary evidence indicates that this peptide does not lyse and thus kill the bacteria by its antimicrobial activity. Bombinin H has antibacterial and hemolytic activity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the skin glands.
Sequence similarities	Belongs to the bombinin family.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Cellular component	Secreted
Domain	Signal
Molecular function	Amphibian defense peptide Antibiotic Antimicrobial
PTM	Amidation Cleavage on pair of basic residues D-amino acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW hemolysis by symbiont of host erythrocytes Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Peptide	19 – 43	25	Acidic peptide 1-1 Potential		PRO_0000003067
	Peptide	44 – 70	27	Bombinin-like peptide 1		PRO_0000003068
	Peptide	74 – 81	8	Octapeptide 1 Potential		PRO_0000003069
	Peptide	84 – 114	31	Acidic peptide 1-2 Potential		PRO_0000003070
	Peptide	117 – 136	20	Bombinin-H Ref.2		PRO_0000003071
Amino acid modifications
	Modified residue	70	1	Asparagine amide
	Modified residue	118	1	D-allo-isoleucine Ref.2
	Modified residue	136	1	Isoleucine amide
Natural variations
	Natural variant	117	1	I → L
	Natural variant	124	1	L → M

Sequences

	Sequence		Length	Mass (Da)
	P29006-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 3EC3EF6E47CA1F92	FASTA	137	14,982
	10 20 30 40 50 60 MNFKYIVAVS ILIASAYARS EENDIQSLSQ RDVLEEESLR EIRGIGGALL SAAKVGLKGL 70 80 90 100 110 120 AKGLAEHFAN GKRTAEEREV MKRLEAAMRD LDSFEHPEEA SEKETRGFNQ EEKEKRIIGP 130 VLGLVGSALG GLLKKIG

References

[1]	"A family of bombinin-related peptides from the skin of Bombina variegata." Simmaco M., Barra D., Chiarini F., Noviello L., Melchiorri P., Kreil G., Richter K. Eur. J. Biochem. 199:217-222(1991) [PubMed: 1712299] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PRELIMINARY PROTEIN SEQUENCE OF 44-69. Tissue: Skin and Skin secretion.
[2]	"Antibacterial and haemolytic peptides containing D-alloisoleucine from the skin of Bombina variegata." Mignogna G., Simmaco M., Kreil G., Barra D. EMBO J. 12:4829-4832(1993) [PubMed: 8223491] [Abstract] Cited for: PROTEIN SEQUENCE OF 117-136, D-AMINO ACID AT ILE-118, AMIDATION AT ILE-136. Tissue: Skin secretion.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59695 mRNA. Translation: CAA42216.1.
PIR	S16222.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P29006.
Family and domain databases
InterPro	IPR007962. Bombinin. [Graphical view]
Pfam	PF05298. Bombinin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BMNL1_BOMVA

Accession

Primary (citable) accession number: P29006

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)