P29001 (INVA_VIGRR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acid beta-fructofuranosidase
EC=3.2.1.26

Alternative name(s):
Acid invertase
Short name=AI
Acid sucrose hydrolase
Vacuolar invertase

Cleaved into the following 2 chains:

1. Acid beta-fructofuranosidase 30 kDa subunit
2. Acid beta-fructofuranosidase 38 kDa subunit

Gene names

Name:

INVA

Organism

Vigna radiata var. radiata (Mung bean) (Phaseolus aureus)

Taxonomic identifier

3916 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna › Vigna radiata

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible role in the continued mobilization of sucrose to sink organs.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subunit structure	Present in two forms, a 70 kDa monomer and a heterodimer of the 30 kDa and 38 kDa subunits. The ratio of the levels of the two forms within cells appears to be regulated developmentally.
Subcellular location	Vacuole Probable.
Developmental stage	Appears after germination and maintained at high levels in rapidly growing tissues.
Induction	Regulation of synthesis appears to be related to the growth of seedlings.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	sucrose metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Propeptide	37 – 101	65			PRO_0000033386
	Chain	102 – 328	227	Acid beta-fructofuranosidase 30 kDa subunit		PRO_0000033387
	Chain	329 – 649	321	Acid beta-fructofuranosidase 38 kDa subunit		PRO_0000033388
Regions
	Region	127 – 130	4	Substrate binding By similarity
	Region	189 – 190	2	Substrate binding By similarity
	Region	253 – 254	2	Substrate binding By similarity
Sites
	Active site	130	1	By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	308	1	Substrate By similarity
	Binding site	341	1	Substrate By similarity
Amino acid modifications
	Glycosylation	210	1	N-linked (GlcNAc...) (complex) Potential
	Glycosylation	275	1	N-linked (GlcNAc...) (complex) Potential
	Glycosylation	618	1	N-linked (GlcNAc...) (high mannose) Potential
	Disulfide bond	498 ↔ 546		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29001 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 0E0375B8C60017B9	FASTA	649	72,167
	10 20 30 40 50 60 MEHHKPLLPT SSHAAPTSST RKDLLFVLCG LLFLSSLVAY GGYRASGVPH AHLSSPTSNH 70 80 90 100 110 120 QQDHQSPTSL PSSKWYPVSR GVSSGVSEKS SNLLFAGEGG ASEAFPWDNS MLSWQRTSFH 130 140 150 160 170 180 FQPEKNWMND PNGPMYYKGW YHFFYQYNPN GAVWGDIVWG HAVSRDMIHW LHLPLAMVAD 190 200 210 220 230 240 QWYDKQGVWT GSATILPNGE IIMLYTGSTN ESVQVQNLAY PADPSDPLLL DWIKHTGNPV 250 260 270 280 290 300 LVPPPGIGAK DFRDPTTAWL TSEGKWRITI GSKLNKTGIA LVYDTEDFKT YELKEGLLRA 310 320 330 340 350 360 VPGTGMWECV DFFPVSKKNG NGLDTSVNGA EVKHVMKVSL DDDRHDYYAI GTYDDNKVLF 370 380 390 400 410 420 TPDDVKNDVG VGLRYDYGIF YASKTFYDQN KDRRILWGWI GESDSEYADV TKGWASVQSI 430 440 450 460 470 480 PRTVRLDTKT GSNLLQWPVD EVESLRLRSD EFKSLKAKPG SVVSLDIETA TQLDVVAEFE 490 500 510 520 530 540 IDTESLEKTA ESNEEFTCSS SGGAAQRGAL GPFGLLVLAD EGLSEYTPVY FYVIKGRNGN 550 560 570 580 590 600 LRTSFCSDQS RSSQANDVRK QIFGSVVPVL KGEKFSLRML VDHSIVESFA QGGRTCVTSR 610 620 630 640 VYPTKAIYGA ARLFLFNNAT EATVTASLKV WQMNSAFIRP FPFNPDQKS

References

[1]

"Cloning and sequence of cDNAs for an intracellular acid invertase from etiolated hypocotyls of mung bean and expression of the gene during growth of seedlings." Arai M., Mori H., Imaseki H. Plant Cell Physiol. 33:245-252(1992) Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 329-360. Tissue: Hypocotyl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10265 mRNA. Translation: BAA01107.1.
3D structure databases
ProteinModelPortal	P29001.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00238.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR021792. Beta-fructofuranosidase. IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF11837. DUF3357. 1 hit. PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INVA_VIGRR

Accession

Primary (citable) accession number: P29001

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	March 6, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program