P29000 (INVA_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acid beta-fructofuranosidase
EC=3.2.1.26

Alternative name(s):
Acid invertase
Short name=AI
Acid sucrose hydrolase
Vacuolar invertase

Gene names

Name:	TIV1
Synonyms:	AIV-1

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Vacuole.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sucrose metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 47	47	Potential
	Propeptide	48 – 92	45			PRO_0000033374
	Chain	93 – 636	544	Acid beta-fructofuranosidase		PRO_0000033375
Sites
	Active site	118	1	By similarity
Amino acid modifications
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	211	1	N-linked (GlcNAc...) Potential
	Glycosylation	276	1	N-linked (GlcNAc...) Potential
	Glycosylation	608	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	542 – 553	12	SSEAP…GKQVY → FAFLSGTINLSL Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P29000 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 74C46246FA93974B	FASTA	636	70,097
	10 20 30 40 50 60 MATQCYDPEN SASRYTLLPD QPDSGHRKSL KIISGIFLSV FLLLSVAFFP ILNNQSPDLQ 70 80 90 100 110 120 IDSRSPAPPS RGVSQGVSDK TFRDVAGASH VSYAWSNAML SWQRTAYHFQ PQKNWMNDPN 130 140 150 160 170 180 GPLYHKGWYH LFYQYNPDSA IWGNITWGHA VSKDLIHWLY LPFAMVPDQW YDINGVWTGS 190 200 210 220 230 240 ATILPDGQIM MLYTGDTDDY VQVQNLAYPA NLSDPLLLDW VKFKGNPVLV PPPGIGVKDF 250 260 270 280 290 300 RDPTTAWTGP QNGQWLLTIG SKIGKTGVAL VYETSNFTSF KLLDGVLHAV PGTGMWECVD 310 320 330 340 350 360 FYPVSTKKTN GLDTSYNGPG VKHVLKASLD DNKQDHYAIG TYDLGKNKWT PDNPELDCGI 370 380 390 400 410 420 GLRLDYGKYY ASKTFYDPKK ERRVLWGWIG ETDSESADLQ KGWASVQSIP RTVLYDKKTG 430 440 450 460 470 480 THLLQWPVEE IESLRVGDPT VKQVDLQPGS IELLRVDSAA ELDIEASFEV DKVALQGIIE 490 500 510 520 530 540 ADHVGFSCST SGGAASRGIL GPFGVIVIAD QTLSELTPVY FYISKGADGR AETHFCADQT 550 560 570 580 590 600 RSSEAPGVGK QVYGSSVPVL DGEKHSMRLL VDHSIVESFA QGGRTVITSR IYPTKAVNGA 610 620 630 ARLFVFNNAT GASVTASVKI WSLESANIQS FPLQDL

References

[1]	"Isolation and characterization of fruit vacuolar invertase genes from two tomato species and temporal differences in mRNA levels during fruit ripening." Elliott K.J., Butler W.O., Dickinson C.D., Konno Y., Vedvick T.S., Fitzmaurice L., Mirkov T.E. Plant Mol. Biol. 21:515-524(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. UC82B and Wild-type.
[2]	Klann E.M., Yelle S., Bennett A.B. Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"A novel cDNA clone for acid invertase in tomato fruit." Ohyama A., Hirai M., Nishimura S. Jpn. J. Genet. 67:491-492(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-553. Tissue: Fruit.
[4]	Davies K.M., Grierson D. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 474-636. Strain: cv. Ailsa Craig. Tissue: Fruit.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z12025 mRNA. Translation: CAA78060.1. Z12026 mRNA. Translation: CAA78061.1. Z12027 Genomic DNA. Translation: CAA78062.1. Z12028 Genomic DNA. Translation: CAA78063.1. M81081 mRNA. Translation: AAA34132.1. D11350 mRNA. Translation: BAA01954.1. X77264 mRNA. Translation: CAA54480.1.
PIR	S31155.
RefSeq	NP_001234618.1. NM_001247689.1.
UniGene	Les.10326.
3D structure databases
ProteinModelPortal	P29000.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100125905.
Enzyme and pathway databases
SABIO-RK	P29000.
UniPathway	UPA00238.
Family and domain databases
Gene3D	2.115.10.20. 1 hit. 2.60.120.560. 1 hit.
InterPro	IPR021792. Beta-fructofuranosidase. IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF11837. DUF3357. 1 hit. PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INVA_SOLLC

Accession

Primary (citable) accession number: P29000

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program