Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P28998

- DHE4_CHLSO

UniProt

P28998 - DHE4_CHLSO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NADP-specific glutamate dehydrogenase

Gene
N/A
Organism
Chlorella sorokiniana
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei202 – 2021PROSITE-ProRule annotation

GO - Molecular functioni

  1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular amino acid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
OrganismiChlorella sorokiniana
Taxonomic identifieri3076 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaTrebouxiophyceaeChlorellalesChlorellaceaeChlorella

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 523›523NADP-specific glutamate dehydrogenasePRO_0000182782Add
BLAST

Post-translational modificationi

The N-termini of the alpha and the beta chains are blocked.

Proteomic databases

PRIDEiP28998.

Expressioni

Inductioni

By ammonium.

Interactioni

Subunit structurei

Homo- and heterohexamer of alpha and beta subunits. Both subunits are encoded by the same gene.

Structurei

3D structure databases

ProteinModelPortaliP28998.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P28998-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
CRPPSSPSLS WPPGLRSPAL PRAVACARGR SAKRDVAAKR LRSRSPRMDA
60 70 80 90 100
TTGDFTALQK AVKQMATKAG TEGLVHGIKN PELRQLLTEI FMKDPEQQEF
110 120 130 140 150
MQAVREVAVS LQPVFEKRPE LLPIFKQIVE PERVITFRVS WLDDAGNLQV
160 170 180 190 200
NRGFRVQYSS AIGPYKGGLR FHPSVNLSIM KFLAFEQIFK NSLTTLPMGG
210 220 230 240 250
GKGGSDFDPK GKSDAEVMRF CQSFMTELQR HISYVQDVPA GDIGVGAREI
260 270 280 290 300
GYLFGQYKRI TKNYTGVLTG KGQEYGGSEI RPEATGYGAV LFVENVLKDK
310 320 330 340 350
GESLKGKRCL VSGAGNVAQY CAELLLEKGA IVLSLSDSQG YVYEPNGFTR
360 370 380 390 400
EQLQAVQDMK KKNNSARISE YKSDTAVYVG DRRKPWELDC QVDIAFPCAT
410 420 430 440 450
QNEIDEHDAE LLIKHGCQYV VEGANMPSTN EAIHKYNKAG IIYCPGKAAN
460 470 480 490 500
AGGVAVSGLE MTQNRMSLNW TREEVRDKLE RIMKDIYDSA MGPSREYNVD
510 520
LAAGANIAGF TKVADAVKAQ GAV
Length:523
Mass (Da):57,530
Last modified:December 1, 1992 - v1
Checksum:iA35FE730E5FEF974
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti220 – 2212FC → LW in CAA41635. (PubMed:1718478)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58832 mRNA. Translation: CAA41636.1.
X58831 Genomic DNA. Translation: CAA41635.1.
PIRiS17949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58832 mRNA. Translation: CAA41636.1 .
X58831 Genomic DNA. Translation: CAA41635.1 .
PIRi S17949.

3D structure databases

ProteinModelPortali P28998.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P28998.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000185. Glu_DH. 1 hit.
PRINTSi PR00082. GLFDHDRGNASE.
SMARTi SM00839. ELFV_dehydrog. 1 hit.
[Graphical view ]
PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana."
    Cock J.M., Kim K.D., Miller P.W., Hutson R.G., Schmidt R.R.
    Plant Mol. Biol. 17:1023-1044(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Entry informationi

Entry nameiDHE4_CHLSO
AccessioniPrimary (citable) accession number: P28998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3