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P28998

- DHE4_CHLSO

UniProt

P28998 - DHE4_CHLSO

Protein

NADP-specific glutamate dehydrogenase

Gene
N/A
Organism
Chlorella sorokiniana
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei202 – 2021PROSITE-ProRule annotation

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular amino acid metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    OrganismiChlorella sorokiniana
    Taxonomic identifieri3076 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaTrebouxiophyceaeChlorellalesChlorellaceaeChlorella

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 523›523NADP-specific glutamate dehydrogenasePRO_0000182782Add
    BLAST

    Post-translational modificationi

    The N-termini of the alpha and the beta chains are blocked.

    Proteomic databases

    PRIDEiP28998.

    Expressioni

    Inductioni

    By ammonium.

    Interactioni

    Subunit structurei

    Homo- and heterohexamer of alpha and beta subunits. Both subunits are encoded by the same gene.

    Structurei

    3D structure databases

    ProteinModelPortaliP28998.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P28998-1 [UniParc]FASTAAdd to Basket

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    CRPPSSPSLS WPPGLRSPAL PRAVACARGR SAKRDVAAKR LRSRSPRMDA    50
    TTGDFTALQK AVKQMATKAG TEGLVHGIKN PELRQLLTEI FMKDPEQQEF 100
    MQAVREVAVS LQPVFEKRPE LLPIFKQIVE PERVITFRVS WLDDAGNLQV 150
    NRGFRVQYSS AIGPYKGGLR FHPSVNLSIM KFLAFEQIFK NSLTTLPMGG 200
    GKGGSDFDPK GKSDAEVMRF CQSFMTELQR HISYVQDVPA GDIGVGAREI 250
    GYLFGQYKRI TKNYTGVLTG KGQEYGGSEI RPEATGYGAV LFVENVLKDK 300
    GESLKGKRCL VSGAGNVAQY CAELLLEKGA IVLSLSDSQG YVYEPNGFTR 350
    EQLQAVQDMK KKNNSARISE YKSDTAVYVG DRRKPWELDC QVDIAFPCAT 400
    QNEIDEHDAE LLIKHGCQYV VEGANMPSTN EAIHKYNKAG IIYCPGKAAN 450
    AGGVAVSGLE MTQNRMSLNW TREEVRDKLE RIMKDIYDSA MGPSREYNVD 500
    LAAGANIAGF TKVADAVKAQ GAV 523
    Length:523
    Mass (Da):57,530
    Last modified:December 1, 1992 - v1
    Checksum:iA35FE730E5FEF974
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Sequence conflicti220 – 2212FC → LW in CAA41635. (PubMed:1718478)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58832 mRNA. Translation: CAA41636.1.
    X58831 Genomic DNA. Translation: CAA41635.1.
    PIRiS17949.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58832 mRNA. Translation: CAA41636.1 .
    X58831 Genomic DNA. Translation: CAA41635.1 .
    PIRi S17949.

    3D structure databases

    ProteinModelPortali P28998.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P28998.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana."
      Cock J.M., Kim K.D., Miller P.W., Hutson R.G., Schmidt R.R.
      Plant Mol. Biol. 17:1023-1044(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

    Entry informationi

    Entry nameiDHE4_CHLSO
    AccessioniPrimary (citable) accession number: P28998
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3