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Protein

NADP-specific glutamate dehydrogenase

Gene
N/A
Organism
Chlorella sorokiniana
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei202 – 2021PROSITE-ProRule annotation

GO - Molecular functioni

  1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular amino acid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.4.1.4. 1334.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
OrganismiChlorella sorokiniana
Taxonomic identifieri3076 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaTrebouxiophyceaeChlorellalesChlorellaceaeChlorella

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 523›523NADP-specific glutamate dehydrogenasePRO_0000182782Add
BLAST

Post-translational modificationi

The N-termini of the alpha and the beta chains are blocked.

Proteomic databases

PRIDEiP28998.

Expressioni

Inductioni

By ammonium.

Interactioni

Subunit structurei

Homo- and heterohexamer of alpha and beta subunits. Both subunits are encoded by the same gene.

Structurei

3D structure databases

ProteinModelPortaliP28998.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P28998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
CRPPSSPSLS WPPGLRSPAL PRAVACARGR SAKRDVAAKR LRSRSPRMDA
60 70 80 90 100
TTGDFTALQK AVKQMATKAG TEGLVHGIKN PELRQLLTEI FMKDPEQQEF
110 120 130 140 150
MQAVREVAVS LQPVFEKRPE LLPIFKQIVE PERVITFRVS WLDDAGNLQV
160 170 180 190 200
NRGFRVQYSS AIGPYKGGLR FHPSVNLSIM KFLAFEQIFK NSLTTLPMGG
210 220 230 240 250
GKGGSDFDPK GKSDAEVMRF CQSFMTELQR HISYVQDVPA GDIGVGAREI
260 270 280 290 300
GYLFGQYKRI TKNYTGVLTG KGQEYGGSEI RPEATGYGAV LFVENVLKDK
310 320 330 340 350
GESLKGKRCL VSGAGNVAQY CAELLLEKGA IVLSLSDSQG YVYEPNGFTR
360 370 380 390 400
EQLQAVQDMK KKNNSARISE YKSDTAVYVG DRRKPWELDC QVDIAFPCAT
410 420 430 440 450
QNEIDEHDAE LLIKHGCQYV VEGANMPSTN EAIHKYNKAG IIYCPGKAAN
460 470 480 490 500
AGGVAVSGLE MTQNRMSLNW TREEVRDKLE RIMKDIYDSA MGPSREYNVD
510 520
LAAGANIAGF TKVADAVKAQ GAV
Length:523
Mass (Da):57,530
Last modified:December 1, 1992 - v1
Checksum:iA35FE730E5FEF974
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti220 – 2212FC → LW in CAA41635 (PubMed:1718478).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58832 mRNA. Translation: CAA41636.1.
X58831 Genomic DNA. Translation: CAA41635.1.
PIRiS17949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58832 mRNA. Translation: CAA41636.1.
X58831 Genomic DNA. Translation: CAA41635.1.
PIRiS17949.

3D structure databases

ProteinModelPortaliP28998.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP28998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.4.1.4. 1334.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana."
    Cock J.M., Kim K.D., Miller P.W., Hutson R.G., Schmidt R.R.
    Plant Mol. Biol. 17:1023-1044(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Entry informationi

Entry nameiDHE4_CHLSO
AccessioniPrimary (citable) accession number: P28998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 1, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.