Reviewed, UniProtKB/Swiss-Prot P28998 (DHE4_CHLSO)

Names and origin

Protein names	Recommended name:     NADP-specific glutamate dehydrogenase       Short name=NADP-GDH     EC=1.4.1.4
Organism	Chlorella sorokiniana
Taxonomic identifier	3076 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Chlorophyta › Trebouxiophyceae › Chlorellales › Chlorellaceae › Chlorella

Protein attributes

Sequence length	523 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homo- and heterohexamer of alpha and beta subunits. Both subunits are encoded by the same gene.
Subcellular location	Plastid › chloroplast.
Induction	By ammonium.
Post-translational modification	The N-termini of the alpha and the beta chains are blocked.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 523	›523	NADP-specific glutamate dehydrogenase		PRO_0000182782
Sites
	Active site	202	1	By similarity
Experimental info
	Sequence conflict	220 – 221	2	FC → LW in CAA41635. Ref.1
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P28998-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: A35FE730E5FEF974	FASTA	523	57,530
	10 20 30 40 50 60 CRPPSSPSLS WPPGLRSPAL PRAVACARGR SAKRDVAAKR LRSRSPRMDA TTGDFTALQK 70 80 90 100 110 120 AVKQMATKAG TEGLVHGIKN PELRQLLTEI FMKDPEQQEF MQAVREVAVS LQPVFEKRPE 130 140 150 160 170 180 LLPIFKQIVE PERVITFRVS WLDDAGNLQV NRGFRVQYSS AIGPYKGGLR FHPSVNLSIM 190 200 210 220 230 240 KFLAFEQIFK NSLTTLPMGG GKGGSDFDPK GKSDAEVMRF CQSFMTELQR HISYVQDVPA 250 260 270 280 290 300 GDIGVGAREI GYLFGQYKRI TKNYTGVLTG KGQEYGGSEI RPEATGYGAV LFVENVLKDK 310 320 330 340 350 360 GESLKGKRCL VSGAGNVAQY CAELLLEKGA IVLSLSDSQG YVYEPNGFTR EQLQAVQDMK 370 380 390 400 410 420 KKNNSARISE YKSDTAVYVG DRRKPWELDC QVDIAFPCAT QNEIDEHDAE LLIKHGCQYV 430 440 450 460 470 480 VEGANMPSTN EAIHKYNKAG IIYCPGKAAN AGGVAVSGLE MTQNRMSLNW TREEVRDKLE 490 500 510 520 RIMKDIYDSA MGPSREYNVD LAAGANIAGF TKVADAVKAQ GAV

References

[1]

"A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana." Cock J.M., Kim K.D., Miller P.W., Hutson R.G., Schmidt R.R. Plant Mol. Biol. 17:1023-1044(1991) [PubMed: 1718478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X58832 mRNA. Translation: CAA41636.1. X58831 Genomic DNA. Translation: CAA41635.1.
PIR	S17949.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.4. 2808.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_CHLSO

Accession

Primary (citable) accession number: P28998

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)