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P28998 (DHE4_CHLSO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
OrganismChlorella sorokiniana
Taxonomic identifier3076 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaTrebouxiophyceaeChlorellalesChlorellaceaeChlorella

Protein attributes

Sequence length523 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homo- and heterohexamer of alpha and beta subunits. Both subunits are encoded by the same gene.

Subcellular location

Plastidchloroplast.

Induction

By ammonium.

Post-translational modification

The N-termini of the alpha and the beta chains are blocked.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processcellular amino acid metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 523›523NADP-specific glutamate dehydrogenase
PRO_0000182782

Sites

Active site2021 By similarity

Experimental info

Sequence conflict220 – 2212FC → LW in CAA41635. Ref.1
Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P28998 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: A35FE730E5FEF974

FASTA52357,530
        10         20         30         40         50         60 
CRPPSSPSLS WPPGLRSPAL PRAVACARGR SAKRDVAAKR LRSRSPRMDA TTGDFTALQK 

        70         80         90        100        110        120 
AVKQMATKAG TEGLVHGIKN PELRQLLTEI FMKDPEQQEF MQAVREVAVS LQPVFEKRPE 

       130        140        150        160        170        180 
LLPIFKQIVE PERVITFRVS WLDDAGNLQV NRGFRVQYSS AIGPYKGGLR FHPSVNLSIM 

       190        200        210        220        230        240 
KFLAFEQIFK NSLTTLPMGG GKGGSDFDPK GKSDAEVMRF CQSFMTELQR HISYVQDVPA 

       250        260        270        280        290        300 
GDIGVGAREI GYLFGQYKRI TKNYTGVLTG KGQEYGGSEI RPEATGYGAV LFVENVLKDK 

       310        320        330        340        350        360 
GESLKGKRCL VSGAGNVAQY CAELLLEKGA IVLSLSDSQG YVYEPNGFTR EQLQAVQDMK 

       370        380        390        400        410        420 
KKNNSARISE YKSDTAVYVG DRRKPWELDC QVDIAFPCAT QNEIDEHDAE LLIKHGCQYV 

       430        440        450        460        470        480 
VEGANMPSTN EAIHKYNKAG IIYCPGKAAN AGGVAVSGLE MTQNRMSLNW TREEVRDKLE 

       490        500        510        520 
RIMKDIYDSA MGPSREYNVD LAAGANIAGF TKVADAVKAQ GAV 

« Hide

References

[1]"A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana."
Cock J.M., Kim K.D., Miller P.W., Hutson R.G., Schmidt R.R.
Plant Mol. Biol. 17:1023-1044(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58832 mRNA. Translation: CAA41636.1.
X58831 Genomic DNA. Translation: CAA41635.1.
PIRS17949.

3D structure databases

ProteinModelPortalP28998.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP28998.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_CHLSO
AccessionPrimary (citable) accession number: P28998
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 16, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families