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P28955

- DEN_EHV1B

UniProt

P28955 - DEN_EHV1B

Protein

Deneddylase

Gene

24

Organism
Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei39 – 391PROSITE-ProRule annotation
    Active sitei174 – 1741PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: InterPro
    2. omega peptidase activity Source: InterPro

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Gene namesi
    Ordered Locus Names:24
    OrganismiEquine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
    Taxonomic identifieri31520 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
    Virus hostiEquus caballus (Horse) [TaxID: 9796]
    ProteomesiUP000001189: Genome

    Subcellular locationi

    Virion tegument Curated. Host nucleus By similarity

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 34213421DeneddylasePRO_0000116036Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP28955.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 238220Peptidase C76PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1171 – 123969Sequence AnalysisAdd
    BLAST
    Coiled coili1493 – 154755Sequence AnalysisAdd
    BLAST
    Coiled coili1619 – 164628Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi319 – 3268Nuclear localization signalBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2798 – 289598Ala-richAdd
    BLAST
    Compositional biasi3166 – 319833Pro-richAdd
    BLAST
    Compositional biasi3212 – 32154Poly-Asp

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR005210. Herpes_UL36.
    IPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    PF03586. Herpes_UL36. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28955-1 [UniParc]FASTAAdd to Basket

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    MAQTLVPANK AGGAQADVVV IGYRNQYDSQ LGEGSHVSCL RSSLSFLRLI     50
    FTHGIDFALT ADSIDGVLVE GRAWTVAGSK SGEAPCMVSI VELPNKITYA 100
    NSANALCCVF SRLYGDSGFY MHPGDGFQST QIPARQFFDG VWKSRSESFA 150
    LITIGAIGLA VYRHGDVAYV FDPHGHGSVT EAFVVRVLAR DVYAYLTGYA 200
    ATDPESDWAG ALVFFVTCGP TESEPGFLIS ATSLLYGISE TYLSDEQYVE 250
    RSVATSHPGI STPPPLTDVA VGAVSEAWQY QELENGAATL DADMEGVAPA 300
    AAQVRASVIR QPTEKRVSLP KRRRPPWTPP TSSENLTTSG NTHTVAGRPS 350
    QKVRNATANV QNPTTGNGSA WAEALNDGGV DNASRPGQAV GAAGTLQNPA 400
    PGDALAMETT QASEEALRTR RVFRLSGEDE APYDLGDAVG VLSAEINELA 450
    TRAEELDVLS STCVDSTVWV TRPHNSPDMD ILEQFITMIF NRLLSFLVEN 500
    GARTRTDSPS VIAGLFPGVL AAIPTQSAVV NLLQATGMAL SDVASYKSIL 550
    NMVSNEDSPV GELAVIKLEL VASEVIKSTQ KLVARVEELE RDVTSGSVNP 600
    LGLYTYLTER LVAEMTKHGG DLFAREPKPG AVSLTERIGS LFRKARTREA 650
    RATRTNASLA RDLNAIEAAV HAAHDKFDAI EIKPADPSDT TNMDELAKSL 700
    DLSAVPTRVA KVIKKVESMV SDSIREYFLR GVQYSARAIA MDKTSGARFQ 750
    VASAAVSNLE RMLDSLPNFE KSLNSVVASA GIQGPPPAQI SGSRKATLLG 800
    NLLRAGQNLT TDNALGAWAA LLSEAHTEGH IERRELEAVI KEITSINDHA 850
    AKKASVEADM ERFRVLSAAV DQATSDMYNS NPHALDTIIR GAEEMIRQAK 900
    VVEAHFDSGR ISREAASRVG VRKREVETLA NSARQRAAEI SAARDEIYSR 950
    LQSLLLPLAG FVGLRAAPGV LEQLAKDAQR STSEELRNLM HEAPKQVVST 1000
    VHSHLWSLFG QFREALEHPN STTSSALAGV GPAFAIVVRS LLDPNKQRES 1050
    VEFFITHADA LADTVGAVEA NPNSELAVAH AVNSIAAAIQ TVSVGGRTIT 1100
    EFAFLVPMLE RYQSRLTIVR ETQRLATAQR AVAASVSAAA EVTTKLRAVA 1150
    VPGVQEDVLK AAIAAAKHVS SEVTAAATAA ERELARLDSK ALSVAQVARA 1200
    HQDLQKQTAV AKQRVGEIEE VLANLNKQQR ELQDRAVHDR WKSDLLAALD 1250
    KIETKSSFDV SELSRLRDLG AARGYDSREF AKRAEQALAA NARAVIAVLD 1300
    NVFKFNPYAP VNSKKETNPT ISMLYNISWW DDFTLAAPIL NTLFAGVDVE 1350
    ELMSLMRIST GMITFASTNG GRPKYNEAVN SLSSDMLKVP QLAKYVDFYG 1400
    KWYTEFNAEM DVLSKLRADV LQAVGVRSGE ISRALEEVTY VRNAEVAEKV 1450
    LADGVKLYIP SDALIAKAVK YLEEFNQKRF AGSAFEEAIA TTIRQDLSTA 1500
    REAATQAEAA RSEAMHRATH ILREVVEAAK AADRDASANL ANLKNLLRLT 1550
    PPPQSVAAAL DKATSSDDIV TQAALLLGTV ESTPELDIKA VEWLQQARSI 1600
    IDSHPLTTKI DGKGPMDPYA ERIEKLHTLR GELDELRRQL TATEVSWDEA 1650
    WGNFSRAVPR ADVSMDGFVD AHQRARTLQA SMGVISEMRA DNKYGRLPPK 1700
    VIGAIESKFA ERHKNLETFN DTSTVLQTAI TQFDSLVQQI PPEMEYDVLR 1750
    SLLASFDQLA AVLPKWVGAE YAAYRSLLLM RIGLYDEYQK IAGIAAAGSR 1800
    PHLEAVEYRS AVEDANLRRA SRVSSLMGDK DVILSLREAK SSIDTAFPQV 1850
    LLDAKGVPVE YRVCYRAVGD KLAAMLCGKL GVSMRPAMPS DPIVESSSVS 1900
    GINVTHDILQ LRFGLEKAYH SGFSTFARFV RHKRADWSPT EPAQAAAEIY 1950
    AAVLATTLTR EYGATWHRIR FMASSGLFVA SPDSVCDTQG GRGKKSNNIV 2000
    HLTLSDVVLS AMLRNSMHLV NFMRLDLTRQ HEYMARTITP VLTKSLLSDI 2050
    LINTLVPTDT STQWRSLPLA GDLEDLAQGM LFSIRMSDWK QNSFSTTSLL 2100
    DVWMRSPGES GRAAAAKIAS AIPGNPLATF TVLARMCIPP NALASLWEAL 2150
    QPEAFSQQNL SYDDVVTSRL DIASTVQTSV AVDPEMKSVD TKSRKQLYTT 2200
    TGTSTTFTLA GSAPSAVKEV SALDVATCAL MFGAPVVIAM ETPEMFSEAS 2250
    GMSFCLKIFD SRPGATDHEI IQAVSSDLSS WGTSLLALDP NAIENACLTT 2300
    QLEILSGLVA SKLLAPAPPC LIVLDPSMRV IKVLWESESP PNDLVITLAE 2350
    DEIIAELPYL NADDDLLPPM NPDDPIYTRV ISGTNIPTAT TEGSLFADQQ 2400
    LEFLRPESNP FPFASHDSSQ SLDVPSSPSS GSDKYEEDPT GIVYDAPVDD 2450
    MSDMAMNKAK AWQEWLEDGF AEDDYRELSN AMPAPPKTTP VVESKQKSDS 2500
    VDRAPTLPPK AAPLPPSDAS AIMSGKPVFK YTPGNKSAVP PSVPAPPTLP 2550
    PAPPLPQSTS KAASGPPPTL PPAPPLPQST SKAASGPPPT LPPAPPLPQS 2600
    TSKAASGPPP TLPPAPPLPQ STSKAASGAT QSDSGKTLTL DVPKTQSKDK 2650
    VVPVPPTDKP STTTPAALKQ SDASKPPTAA IQHQQKLGTP VTPKDSGDKP 2700
    TDNASAPVGV SPVTPDGTPG AKPPPKDAPP VDDTKQPVRK SLPSQVRGGR 2750
    PYIRPSLGPF KFTGPPGYTI PVHGLPPSDS NVTQSTKEPP KPAVETPAAA 2800
    PAKSAAAPAA APAKSAAAPA AAPAKSAAAP AAAPAKSAAA PAAAPAKSAA 2850
    APAAAPAKDQ TKSAAEVPKP AKDQAKDQAK DQAKDQAKDQ AKDQAKSTTG 2900
    QKLAKDPKSD GLTDDVALEI VPEKTPLPDD SPIGAVPENT PLPDDSPIGS 2950
    PDLSASKNSH TTDAVSSDRF SVACKVPLPD SPEDDFYSYA VDVPLPDSPT 3000
    DDPSSGRSDA RAPTVGGVAS IHRKSDSRNN RQSDAWRRAF ADTLHGRPRN 3050
    RSATKPCKSA PYKVPHAISY TKIPSVPNDQ SGLAGKPCSE EPKRPTGRDT 3100
    PVGSWNVSPS QAPADIPTAI PQNQNTSESP RTTSLKSPTR TVQSSMPADD 3150
    IDELAEYDLQ IARAVPVTKH PQPPPANQTP PPQEPPAPID DRKNIRPPLS 3200
    EEEIIAFLIN MDDDDAGNAS GPVDLHSVQA PKLPKQSKPT TNQFVPLDWW 3250
    TETEPVVDAD SLDLSPKQQR LFSWESTRDL LNINVRDRVY EEESDDEYTV 3300
    SWDQHLVPAV SPTSVSSYSS DTVTDSYTDI NDPRSVVCPL DGNAQNNVRE 3350
    FLDTHSSRVR VVPADELLSR RYFRSTSLSA MALLIAACRT IVRRLRATRR 3400
    VLTDINRSLL LDLKQIRVLL G 3421
    Length:3,421
    Mass (Da):367,083
    Last modified:December 1, 1992 - v1
    Checksum:i5075EFE4739BB7AC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY665713 Genomic DNA. Translation: AAT67281.1.
    PIRiG36797. WZBEB6.
    RefSeqiYP_053069.1. NC_001491.2.

    Genome annotation databases

    GeneIDi1487490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY665713 Genomic DNA. Translation: AAT67281.1 .
    PIRi G36797. WZBEB6.
    RefSeqi YP_053069.1. NC_001491.2.

    3D structure databases

    ProteinModelPortali P28955.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1487490.

    Family and domain databases

    InterProi IPR005210. Herpes_UL36.
    IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    PF03586. Herpes_UL36. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_EHV1B
    AccessioniPrimary (citable) accession number: P28955
    Secondary accession number(s): Q6DLI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3