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P28955

- DEN_EHV1B

UniProt

P28955 - DEN_EHV1B

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Protein
Deneddylase
Gene
24
Organism
Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei39 – 391 By similarity
Active sitei174 – 1741 Reviewed prediction

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Gene namesi
Ordered Locus Names:24
OrganismiEquine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Taxonomic identifieri31520 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
ProteomesiUP000001189: Genome

Subcellular locationi

Virion tegument Reviewed prediction. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 34213421Deneddylase
PRO_0000116036Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Structurei

3D structure databases

ProteinModelPortaliP28955.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 238220Peptidase C76
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1171 – 123969 Reviewed prediction
Add
BLAST
Coiled coili1493 – 154755 Reviewed prediction
Add
BLAST
Coiled coili1619 – 164628 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi319 – 3268Nuclear localization signal By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2798 – 289598Ala-rich
Add
BLAST
Compositional biasi3166 – 319833Pro-rich
Add
BLAST
Compositional biasi3212 – 32154Poly-Asp

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28955-1 [UniParc]FASTAAdd to Basket

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MAQTLVPANK AGGAQADVVV IGYRNQYDSQ LGEGSHVSCL RSSLSFLRLI     50
FTHGIDFALT ADSIDGVLVE GRAWTVAGSK SGEAPCMVSI VELPNKITYA 100
NSANALCCVF SRLYGDSGFY MHPGDGFQST QIPARQFFDG VWKSRSESFA 150
LITIGAIGLA VYRHGDVAYV FDPHGHGSVT EAFVVRVLAR DVYAYLTGYA 200
ATDPESDWAG ALVFFVTCGP TESEPGFLIS ATSLLYGISE TYLSDEQYVE 250
RSVATSHPGI STPPPLTDVA VGAVSEAWQY QELENGAATL DADMEGVAPA 300
AAQVRASVIR QPTEKRVSLP KRRRPPWTPP TSSENLTTSG NTHTVAGRPS 350
QKVRNATANV QNPTTGNGSA WAEALNDGGV DNASRPGQAV GAAGTLQNPA 400
PGDALAMETT QASEEALRTR RVFRLSGEDE APYDLGDAVG VLSAEINELA 450
TRAEELDVLS STCVDSTVWV TRPHNSPDMD ILEQFITMIF NRLLSFLVEN 500
GARTRTDSPS VIAGLFPGVL AAIPTQSAVV NLLQATGMAL SDVASYKSIL 550
NMVSNEDSPV GELAVIKLEL VASEVIKSTQ KLVARVEELE RDVTSGSVNP 600
LGLYTYLTER LVAEMTKHGG DLFAREPKPG AVSLTERIGS LFRKARTREA 650
RATRTNASLA RDLNAIEAAV HAAHDKFDAI EIKPADPSDT TNMDELAKSL 700
DLSAVPTRVA KVIKKVESMV SDSIREYFLR GVQYSARAIA MDKTSGARFQ 750
VASAAVSNLE RMLDSLPNFE KSLNSVVASA GIQGPPPAQI SGSRKATLLG 800
NLLRAGQNLT TDNALGAWAA LLSEAHTEGH IERRELEAVI KEITSINDHA 850
AKKASVEADM ERFRVLSAAV DQATSDMYNS NPHALDTIIR GAEEMIRQAK 900
VVEAHFDSGR ISREAASRVG VRKREVETLA NSARQRAAEI SAARDEIYSR 950
LQSLLLPLAG FVGLRAAPGV LEQLAKDAQR STSEELRNLM HEAPKQVVST 1000
VHSHLWSLFG QFREALEHPN STTSSALAGV GPAFAIVVRS LLDPNKQRES 1050
VEFFITHADA LADTVGAVEA NPNSELAVAH AVNSIAAAIQ TVSVGGRTIT 1100
EFAFLVPMLE RYQSRLTIVR ETQRLATAQR AVAASVSAAA EVTTKLRAVA 1150
VPGVQEDVLK AAIAAAKHVS SEVTAAATAA ERELARLDSK ALSVAQVARA 1200
HQDLQKQTAV AKQRVGEIEE VLANLNKQQR ELQDRAVHDR WKSDLLAALD 1250
KIETKSSFDV SELSRLRDLG AARGYDSREF AKRAEQALAA NARAVIAVLD 1300
NVFKFNPYAP VNSKKETNPT ISMLYNISWW DDFTLAAPIL NTLFAGVDVE 1350
ELMSLMRIST GMITFASTNG GRPKYNEAVN SLSSDMLKVP QLAKYVDFYG 1400
KWYTEFNAEM DVLSKLRADV LQAVGVRSGE ISRALEEVTY VRNAEVAEKV 1450
LADGVKLYIP SDALIAKAVK YLEEFNQKRF AGSAFEEAIA TTIRQDLSTA 1500
REAATQAEAA RSEAMHRATH ILREVVEAAK AADRDASANL ANLKNLLRLT 1550
PPPQSVAAAL DKATSSDDIV TQAALLLGTV ESTPELDIKA VEWLQQARSI 1600
IDSHPLTTKI DGKGPMDPYA ERIEKLHTLR GELDELRRQL TATEVSWDEA 1650
WGNFSRAVPR ADVSMDGFVD AHQRARTLQA SMGVISEMRA DNKYGRLPPK 1700
VIGAIESKFA ERHKNLETFN DTSTVLQTAI TQFDSLVQQI PPEMEYDVLR 1750
SLLASFDQLA AVLPKWVGAE YAAYRSLLLM RIGLYDEYQK IAGIAAAGSR 1800
PHLEAVEYRS AVEDANLRRA SRVSSLMGDK DVILSLREAK SSIDTAFPQV 1850
LLDAKGVPVE YRVCYRAVGD KLAAMLCGKL GVSMRPAMPS DPIVESSSVS 1900
GINVTHDILQ LRFGLEKAYH SGFSTFARFV RHKRADWSPT EPAQAAAEIY 1950
AAVLATTLTR EYGATWHRIR FMASSGLFVA SPDSVCDTQG GRGKKSNNIV 2000
HLTLSDVVLS AMLRNSMHLV NFMRLDLTRQ HEYMARTITP VLTKSLLSDI 2050
LINTLVPTDT STQWRSLPLA GDLEDLAQGM LFSIRMSDWK QNSFSTTSLL 2100
DVWMRSPGES GRAAAAKIAS AIPGNPLATF TVLARMCIPP NALASLWEAL 2150
QPEAFSQQNL SYDDVVTSRL DIASTVQTSV AVDPEMKSVD TKSRKQLYTT 2200
TGTSTTFTLA GSAPSAVKEV SALDVATCAL MFGAPVVIAM ETPEMFSEAS 2250
GMSFCLKIFD SRPGATDHEI IQAVSSDLSS WGTSLLALDP NAIENACLTT 2300
QLEILSGLVA SKLLAPAPPC LIVLDPSMRV IKVLWESESP PNDLVITLAE 2350
DEIIAELPYL NADDDLLPPM NPDDPIYTRV ISGTNIPTAT TEGSLFADQQ 2400
LEFLRPESNP FPFASHDSSQ SLDVPSSPSS GSDKYEEDPT GIVYDAPVDD 2450
MSDMAMNKAK AWQEWLEDGF AEDDYRELSN AMPAPPKTTP VVESKQKSDS 2500
VDRAPTLPPK AAPLPPSDAS AIMSGKPVFK YTPGNKSAVP PSVPAPPTLP 2550
PAPPLPQSTS KAASGPPPTL PPAPPLPQST SKAASGPPPT LPPAPPLPQS 2600
TSKAASGPPP TLPPAPPLPQ STSKAASGAT QSDSGKTLTL DVPKTQSKDK 2650
VVPVPPTDKP STTTPAALKQ SDASKPPTAA IQHQQKLGTP VTPKDSGDKP 2700
TDNASAPVGV SPVTPDGTPG AKPPPKDAPP VDDTKQPVRK SLPSQVRGGR 2750
PYIRPSLGPF KFTGPPGYTI PVHGLPPSDS NVTQSTKEPP KPAVETPAAA 2800
PAKSAAAPAA APAKSAAAPA AAPAKSAAAP AAAPAKSAAA PAAAPAKSAA 2850
APAAAPAKDQ TKSAAEVPKP AKDQAKDQAK DQAKDQAKDQ AKDQAKSTTG 2900
QKLAKDPKSD GLTDDVALEI VPEKTPLPDD SPIGAVPENT PLPDDSPIGS 2950
PDLSASKNSH TTDAVSSDRF SVACKVPLPD SPEDDFYSYA VDVPLPDSPT 3000
DDPSSGRSDA RAPTVGGVAS IHRKSDSRNN RQSDAWRRAF ADTLHGRPRN 3050
RSATKPCKSA PYKVPHAISY TKIPSVPNDQ SGLAGKPCSE EPKRPTGRDT 3100
PVGSWNVSPS QAPADIPTAI PQNQNTSESP RTTSLKSPTR TVQSSMPADD 3150
IDELAEYDLQ IARAVPVTKH PQPPPANQTP PPQEPPAPID DRKNIRPPLS 3200
EEEIIAFLIN MDDDDAGNAS GPVDLHSVQA PKLPKQSKPT TNQFVPLDWW 3250
TETEPVVDAD SLDLSPKQQR LFSWESTRDL LNINVRDRVY EEESDDEYTV 3300
SWDQHLVPAV SPTSVSSYSS DTVTDSYTDI NDPRSVVCPL DGNAQNNVRE 3350
FLDTHSSRVR VVPADELLSR RYFRSTSLSA MALLIAACRT IVRRLRATRR 3400
VLTDINRSLL LDLKQIRVLL G 3421
Length:3,421
Mass (Da):367,083
Last modified:December 1, 1992 - v1
Checksum:i5075EFE4739BB7AC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY665713 Genomic DNA. Translation: AAT67281.1.
PIRiG36797. WZBEB6.
RefSeqiYP_053069.1. NC_001491.2.

Genome annotation databases

GeneIDi1487490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY665713 Genomic DNA. Translation: AAT67281.1 .
PIRi G36797. WZBEB6.
RefSeqi YP_053069.1. NC_001491.2.

3D structure databases

ProteinModelPortali P28955.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1487490.

Family and domain databases

InterProi IPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_EHV1B
AccessioniPrimary (citable) accession number: P28955
Secondary accession number(s): Q6DLI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi