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P28955 (DEN_EHV1B) Reviewed, UniProtKB/Swiss-Prot

Last modified June 26, 2013. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Gene names
Ordered Locus Names:24
OrganismEquine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus) [Complete proteome]
Taxonomic identifier31520 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostEquus caballus (Horse) [TaxID: 9796]

Protein attributes

Sequence length3421 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument Potential. Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 34213421Deneddylase
PRO_0000116036

Regions

Domain19 – 238220Peptidase C76
Coiled coil1171 – 123969 Potential
Coiled coil1493 – 154755 Potential
Coiled coil1619 – 164628 Potential
Motif319 – 3268Nuclear localization signal By similarity
Compositional bias2798 – 289598Ala-rich
Compositional bias3166 – 319833Pro-rich
Compositional bias3212 – 32154Poly-Asp

Sites

Active site391 By similarity
Active site1741 Potential

Sequences

Sequence LengthMass (Da)Tools
P28955 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 5075EFE4739BB7AC

FASTA3,421367,083
        10         20         30         40         50         60 
MAQTLVPANK AGGAQADVVV IGYRNQYDSQ LGEGSHVSCL RSSLSFLRLI FTHGIDFALT 

        70         80         90        100        110        120 
ADSIDGVLVE GRAWTVAGSK SGEAPCMVSI VELPNKITYA NSANALCCVF SRLYGDSGFY 

       130        140        150        160        170        180 
MHPGDGFQST QIPARQFFDG VWKSRSESFA LITIGAIGLA VYRHGDVAYV FDPHGHGSVT 

       190        200        210        220        230        240 
EAFVVRVLAR DVYAYLTGYA ATDPESDWAG ALVFFVTCGP TESEPGFLIS ATSLLYGISE 

       250        260        270        280        290        300 
TYLSDEQYVE RSVATSHPGI STPPPLTDVA VGAVSEAWQY QELENGAATL DADMEGVAPA 

       310        320        330        340        350        360 
AAQVRASVIR QPTEKRVSLP KRRRPPWTPP TSSENLTTSG NTHTVAGRPS QKVRNATANV 

       370        380        390        400        410        420 
QNPTTGNGSA WAEALNDGGV DNASRPGQAV GAAGTLQNPA PGDALAMETT QASEEALRTR 

       430        440        450        460        470        480 
RVFRLSGEDE APYDLGDAVG VLSAEINELA TRAEELDVLS STCVDSTVWV TRPHNSPDMD 

       490        500        510        520        530        540 
ILEQFITMIF NRLLSFLVEN GARTRTDSPS VIAGLFPGVL AAIPTQSAVV NLLQATGMAL 

       550        560        570        580        590        600 
SDVASYKSIL NMVSNEDSPV GELAVIKLEL VASEVIKSTQ KLVARVEELE RDVTSGSVNP 

       610        620        630        640        650        660 
LGLYTYLTER LVAEMTKHGG DLFAREPKPG AVSLTERIGS LFRKARTREA RATRTNASLA 

       670        680        690        700        710        720 
RDLNAIEAAV HAAHDKFDAI EIKPADPSDT TNMDELAKSL DLSAVPTRVA KVIKKVESMV 

       730        740        750        760        770        780 
SDSIREYFLR GVQYSARAIA MDKTSGARFQ VASAAVSNLE RMLDSLPNFE KSLNSVVASA 

       790        800        810        820        830        840 
GIQGPPPAQI SGSRKATLLG NLLRAGQNLT TDNALGAWAA LLSEAHTEGH IERRELEAVI 

       850        860        870        880        890        900 
KEITSINDHA AKKASVEADM ERFRVLSAAV DQATSDMYNS NPHALDTIIR GAEEMIRQAK 

       910        920        930        940        950        960 
VVEAHFDSGR ISREAASRVG VRKREVETLA NSARQRAAEI SAARDEIYSR LQSLLLPLAG 

       970        980        990       1000       1010       1020 
FVGLRAAPGV LEQLAKDAQR STSEELRNLM HEAPKQVVST VHSHLWSLFG QFREALEHPN 

      1030       1040       1050       1060       1070       1080 
STTSSALAGV GPAFAIVVRS LLDPNKQRES VEFFITHADA LADTVGAVEA NPNSELAVAH 

      1090       1100       1110       1120       1130       1140 
AVNSIAAAIQ TVSVGGRTIT EFAFLVPMLE RYQSRLTIVR ETQRLATAQR AVAASVSAAA 

      1150       1160       1170       1180       1190       1200 
EVTTKLRAVA VPGVQEDVLK AAIAAAKHVS SEVTAAATAA ERELARLDSK ALSVAQVARA 

      1210       1220       1230       1240       1250       1260 
HQDLQKQTAV AKQRVGEIEE VLANLNKQQR ELQDRAVHDR WKSDLLAALD KIETKSSFDV 

      1270       1280       1290       1300       1310       1320 
SELSRLRDLG AARGYDSREF AKRAEQALAA NARAVIAVLD NVFKFNPYAP VNSKKETNPT 

      1330       1340       1350       1360       1370       1380 
ISMLYNISWW DDFTLAAPIL NTLFAGVDVE ELMSLMRIST GMITFASTNG GRPKYNEAVN 

      1390       1400       1410       1420       1430       1440 
SLSSDMLKVP QLAKYVDFYG KWYTEFNAEM DVLSKLRADV LQAVGVRSGE ISRALEEVTY 

      1450       1460       1470       1480       1490       1500 
VRNAEVAEKV LADGVKLYIP SDALIAKAVK YLEEFNQKRF AGSAFEEAIA TTIRQDLSTA 

      1510       1520       1530       1540       1550       1560 
REAATQAEAA RSEAMHRATH ILREVVEAAK AADRDASANL ANLKNLLRLT PPPQSVAAAL 

      1570       1580       1590       1600       1610       1620 
DKATSSDDIV TQAALLLGTV ESTPELDIKA VEWLQQARSI IDSHPLTTKI DGKGPMDPYA 

      1630       1640       1650       1660       1670       1680 
ERIEKLHTLR GELDELRRQL TATEVSWDEA WGNFSRAVPR ADVSMDGFVD AHQRARTLQA 

      1690       1700       1710       1720       1730       1740 
SMGVISEMRA DNKYGRLPPK VIGAIESKFA ERHKNLETFN DTSTVLQTAI TQFDSLVQQI 

      1750       1760       1770       1780       1790       1800 
PPEMEYDVLR SLLASFDQLA AVLPKWVGAE YAAYRSLLLM RIGLYDEYQK IAGIAAAGSR 

      1810       1820       1830       1840       1850       1860 
PHLEAVEYRS AVEDANLRRA SRVSSLMGDK DVILSLREAK SSIDTAFPQV LLDAKGVPVE 

      1870       1880       1890       1900       1910       1920 
YRVCYRAVGD KLAAMLCGKL GVSMRPAMPS DPIVESSSVS GINVTHDILQ LRFGLEKAYH 

      1930       1940       1950       1960       1970       1980 
SGFSTFARFV RHKRADWSPT EPAQAAAEIY AAVLATTLTR EYGATWHRIR FMASSGLFVA 

      1990       2000       2010       2020       2030       2040 
SPDSVCDTQG GRGKKSNNIV HLTLSDVVLS AMLRNSMHLV NFMRLDLTRQ HEYMARTITP 

      2050       2060       2070       2080       2090       2100 
VLTKSLLSDI LINTLVPTDT STQWRSLPLA GDLEDLAQGM LFSIRMSDWK QNSFSTTSLL 

      2110       2120       2130       2140       2150       2160 
DVWMRSPGES GRAAAAKIAS AIPGNPLATF TVLARMCIPP NALASLWEAL QPEAFSQQNL 

      2170       2180       2190       2200       2210       2220 
SYDDVVTSRL DIASTVQTSV AVDPEMKSVD TKSRKQLYTT TGTSTTFTLA GSAPSAVKEV 

      2230       2240       2250       2260       2270       2280 
SALDVATCAL MFGAPVVIAM ETPEMFSEAS GMSFCLKIFD SRPGATDHEI IQAVSSDLSS 

      2290       2300       2310       2320       2330       2340 
WGTSLLALDP NAIENACLTT QLEILSGLVA SKLLAPAPPC LIVLDPSMRV IKVLWESESP 

      2350       2360       2370       2380       2390       2400 
PNDLVITLAE DEIIAELPYL NADDDLLPPM NPDDPIYTRV ISGTNIPTAT TEGSLFADQQ 

      2410       2420       2430       2440       2450       2460 
LEFLRPESNP FPFASHDSSQ SLDVPSSPSS GSDKYEEDPT GIVYDAPVDD MSDMAMNKAK 

      2470       2480       2490       2500       2510       2520 
AWQEWLEDGF AEDDYRELSN AMPAPPKTTP VVESKQKSDS VDRAPTLPPK AAPLPPSDAS 

      2530       2540       2550       2560       2570       2580 
AIMSGKPVFK YTPGNKSAVP PSVPAPPTLP PAPPLPQSTS KAASGPPPTL PPAPPLPQST 

      2590       2600       2610       2620       2630       2640 
SKAASGPPPT LPPAPPLPQS TSKAASGPPP TLPPAPPLPQ STSKAASGAT QSDSGKTLTL 

      2650       2660       2670       2680       2690       2700 
DVPKTQSKDK VVPVPPTDKP STTTPAALKQ SDASKPPTAA IQHQQKLGTP VTPKDSGDKP 

      2710       2720       2730       2740       2750       2760 
TDNASAPVGV SPVTPDGTPG AKPPPKDAPP VDDTKQPVRK SLPSQVRGGR PYIRPSLGPF 

      2770       2780       2790       2800       2810       2820 
KFTGPPGYTI PVHGLPPSDS NVTQSTKEPP KPAVETPAAA PAKSAAAPAA APAKSAAAPA 

      2830       2840       2850       2860       2870       2880 
AAPAKSAAAP AAAPAKSAAA PAAAPAKSAA APAAAPAKDQ TKSAAEVPKP AKDQAKDQAK 

      2890       2900       2910       2920       2930       2940 
DQAKDQAKDQ AKDQAKSTTG QKLAKDPKSD GLTDDVALEI VPEKTPLPDD SPIGAVPENT 

      2950       2960       2970       2980       2990       3000 
PLPDDSPIGS PDLSASKNSH TTDAVSSDRF SVACKVPLPD SPEDDFYSYA VDVPLPDSPT 

      3010       3020       3030       3040       3050       3060 
DDPSSGRSDA RAPTVGGVAS IHRKSDSRNN RQSDAWRRAF ADTLHGRPRN RSATKPCKSA 

      3070       3080       3090       3100       3110       3120 
PYKVPHAISY TKIPSVPNDQ SGLAGKPCSE EPKRPTGRDT PVGSWNVSPS QAPADIPTAI 

      3130       3140       3150       3160       3170       3180 
PQNQNTSESP RTTSLKSPTR TVQSSMPADD IDELAEYDLQ IARAVPVTKH PQPPPANQTP 

      3190       3200       3210       3220       3230       3240 
PPQEPPAPID DRKNIRPPLS EEEIIAFLIN MDDDDAGNAS GPVDLHSVQA PKLPKQSKPT 

      3250       3260       3270       3280       3290       3300 
TNQFVPLDWW TETEPVVDAD SLDLSPKQQR LFSWESTRDL LNINVRDRVY EEESDDEYTV 

      3310       3320       3330       3340       3350       3360 
SWDQHLVPAV SPTSVSSYSS DTVTDSYTDI NDPRSVVCPL DGNAQNNVRE FLDTHSSRVR 

      3370       3380       3390       3400       3410       3420 
VVPADELLSR RYFRSTSLSA MALLIAACRT IVRRLRATRR VLTDINRSLL LDLKQIRVLL 


G 

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References

[1]"The DNA sequence of equine herpesvirus-1."
Telford E.A.R., Watson M.S., McBride K., Davison A.J.
Virology 189:304-316(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY665713 Genomic DNA. Translation: AAT67281.1.
PIRWZBEB6. G36797.
RefSeqYP_053069.1. NC_001491.2.

3D structure databases

ProteinModelPortalP28955.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1487490.

Phylogenomic databases

ProtClustDBCLSP2510208.

Family and domain databases

InterProIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_EHV1B
AccessionPrimary (citable) accession number: P28955
Secondary accession number(s): Q6DLI7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 26, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries