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P28955

- DEN_EHV1B

UniProt

P28955 - DEN_EHV1B

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Protein

Deneddylase

Gene

24

Organism
Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei39 – 391PROSITE-ProRule annotation
Active sitei174 – 1741PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Gene namesi
Ordered Locus Names:24
OrganismiEquine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Taxonomic identifieri31520 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
ProteomesiUP000001189: Genome

Subcellular locationi

Virion tegument Curated. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 34213421DeneddylasePRO_0000116036Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Structurei

3D structure databases

ProteinModelPortaliP28955.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 238220Peptidase C76PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1171 – 123969Sequence AnalysisAdd
BLAST
Coiled coili1493 – 154755Sequence AnalysisAdd
BLAST
Coiled coili1619 – 164628Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi319 – 3268Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2798 – 289598Ala-richAdd
BLAST
Compositional biasi3166 – 319833Pro-richAdd
BLAST
Compositional biasi3212 – 32154Poly-Asp

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28955-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MAQTLVPANK AGGAQADVVV IGYRNQYDSQ LGEGSHVSCL RSSLSFLRLI
60 70 80 90 100
FTHGIDFALT ADSIDGVLVE GRAWTVAGSK SGEAPCMVSI VELPNKITYA
110 120 130 140 150
NSANALCCVF SRLYGDSGFY MHPGDGFQST QIPARQFFDG VWKSRSESFA
160 170 180 190 200
LITIGAIGLA VYRHGDVAYV FDPHGHGSVT EAFVVRVLAR DVYAYLTGYA
210 220 230 240 250
ATDPESDWAG ALVFFVTCGP TESEPGFLIS ATSLLYGISE TYLSDEQYVE
260 270 280 290 300
RSVATSHPGI STPPPLTDVA VGAVSEAWQY QELENGAATL DADMEGVAPA
310 320 330 340 350
AAQVRASVIR QPTEKRVSLP KRRRPPWTPP TSSENLTTSG NTHTVAGRPS
360 370 380 390 400
QKVRNATANV QNPTTGNGSA WAEALNDGGV DNASRPGQAV GAAGTLQNPA
410 420 430 440 450
PGDALAMETT QASEEALRTR RVFRLSGEDE APYDLGDAVG VLSAEINELA
460 470 480 490 500
TRAEELDVLS STCVDSTVWV TRPHNSPDMD ILEQFITMIF NRLLSFLVEN
510 520 530 540 550
GARTRTDSPS VIAGLFPGVL AAIPTQSAVV NLLQATGMAL SDVASYKSIL
560 570 580 590 600
NMVSNEDSPV GELAVIKLEL VASEVIKSTQ KLVARVEELE RDVTSGSVNP
610 620 630 640 650
LGLYTYLTER LVAEMTKHGG DLFAREPKPG AVSLTERIGS LFRKARTREA
660 670 680 690 700
RATRTNASLA RDLNAIEAAV HAAHDKFDAI EIKPADPSDT TNMDELAKSL
710 720 730 740 750
DLSAVPTRVA KVIKKVESMV SDSIREYFLR GVQYSARAIA MDKTSGARFQ
760 770 780 790 800
VASAAVSNLE RMLDSLPNFE KSLNSVVASA GIQGPPPAQI SGSRKATLLG
810 820 830 840 850
NLLRAGQNLT TDNALGAWAA LLSEAHTEGH IERRELEAVI KEITSINDHA
860 870 880 890 900
AKKASVEADM ERFRVLSAAV DQATSDMYNS NPHALDTIIR GAEEMIRQAK
910 920 930 940 950
VVEAHFDSGR ISREAASRVG VRKREVETLA NSARQRAAEI SAARDEIYSR
960 970 980 990 1000
LQSLLLPLAG FVGLRAAPGV LEQLAKDAQR STSEELRNLM HEAPKQVVST
1010 1020 1030 1040 1050
VHSHLWSLFG QFREALEHPN STTSSALAGV GPAFAIVVRS LLDPNKQRES
1060 1070 1080 1090 1100
VEFFITHADA LADTVGAVEA NPNSELAVAH AVNSIAAAIQ TVSVGGRTIT
1110 1120 1130 1140 1150
EFAFLVPMLE RYQSRLTIVR ETQRLATAQR AVAASVSAAA EVTTKLRAVA
1160 1170 1180 1190 1200
VPGVQEDVLK AAIAAAKHVS SEVTAAATAA ERELARLDSK ALSVAQVARA
1210 1220 1230 1240 1250
HQDLQKQTAV AKQRVGEIEE VLANLNKQQR ELQDRAVHDR WKSDLLAALD
1260 1270 1280 1290 1300
KIETKSSFDV SELSRLRDLG AARGYDSREF AKRAEQALAA NARAVIAVLD
1310 1320 1330 1340 1350
NVFKFNPYAP VNSKKETNPT ISMLYNISWW DDFTLAAPIL NTLFAGVDVE
1360 1370 1380 1390 1400
ELMSLMRIST GMITFASTNG GRPKYNEAVN SLSSDMLKVP QLAKYVDFYG
1410 1420 1430 1440 1450
KWYTEFNAEM DVLSKLRADV LQAVGVRSGE ISRALEEVTY VRNAEVAEKV
1460 1470 1480 1490 1500
LADGVKLYIP SDALIAKAVK YLEEFNQKRF AGSAFEEAIA TTIRQDLSTA
1510 1520 1530 1540 1550
REAATQAEAA RSEAMHRATH ILREVVEAAK AADRDASANL ANLKNLLRLT
1560 1570 1580 1590 1600
PPPQSVAAAL DKATSSDDIV TQAALLLGTV ESTPELDIKA VEWLQQARSI
1610 1620 1630 1640 1650
IDSHPLTTKI DGKGPMDPYA ERIEKLHTLR GELDELRRQL TATEVSWDEA
1660 1670 1680 1690 1700
WGNFSRAVPR ADVSMDGFVD AHQRARTLQA SMGVISEMRA DNKYGRLPPK
1710 1720 1730 1740 1750
VIGAIESKFA ERHKNLETFN DTSTVLQTAI TQFDSLVQQI PPEMEYDVLR
1760 1770 1780 1790 1800
SLLASFDQLA AVLPKWVGAE YAAYRSLLLM RIGLYDEYQK IAGIAAAGSR
1810 1820 1830 1840 1850
PHLEAVEYRS AVEDANLRRA SRVSSLMGDK DVILSLREAK SSIDTAFPQV
1860 1870 1880 1890 1900
LLDAKGVPVE YRVCYRAVGD KLAAMLCGKL GVSMRPAMPS DPIVESSSVS
1910 1920 1930 1940 1950
GINVTHDILQ LRFGLEKAYH SGFSTFARFV RHKRADWSPT EPAQAAAEIY
1960 1970 1980 1990 2000
AAVLATTLTR EYGATWHRIR FMASSGLFVA SPDSVCDTQG GRGKKSNNIV
2010 2020 2030 2040 2050
HLTLSDVVLS AMLRNSMHLV NFMRLDLTRQ HEYMARTITP VLTKSLLSDI
2060 2070 2080 2090 2100
LINTLVPTDT STQWRSLPLA GDLEDLAQGM LFSIRMSDWK QNSFSTTSLL
2110 2120 2130 2140 2150
DVWMRSPGES GRAAAAKIAS AIPGNPLATF TVLARMCIPP NALASLWEAL
2160 2170 2180 2190 2200
QPEAFSQQNL SYDDVVTSRL DIASTVQTSV AVDPEMKSVD TKSRKQLYTT
2210 2220 2230 2240 2250
TGTSTTFTLA GSAPSAVKEV SALDVATCAL MFGAPVVIAM ETPEMFSEAS
2260 2270 2280 2290 2300
GMSFCLKIFD SRPGATDHEI IQAVSSDLSS WGTSLLALDP NAIENACLTT
2310 2320 2330 2340 2350
QLEILSGLVA SKLLAPAPPC LIVLDPSMRV IKVLWESESP PNDLVITLAE
2360 2370 2380 2390 2400
DEIIAELPYL NADDDLLPPM NPDDPIYTRV ISGTNIPTAT TEGSLFADQQ
2410 2420 2430 2440 2450
LEFLRPESNP FPFASHDSSQ SLDVPSSPSS GSDKYEEDPT GIVYDAPVDD
2460 2470 2480 2490 2500
MSDMAMNKAK AWQEWLEDGF AEDDYRELSN AMPAPPKTTP VVESKQKSDS
2510 2520 2530 2540 2550
VDRAPTLPPK AAPLPPSDAS AIMSGKPVFK YTPGNKSAVP PSVPAPPTLP
2560 2570 2580 2590 2600
PAPPLPQSTS KAASGPPPTL PPAPPLPQST SKAASGPPPT LPPAPPLPQS
2610 2620 2630 2640 2650
TSKAASGPPP TLPPAPPLPQ STSKAASGAT QSDSGKTLTL DVPKTQSKDK
2660 2670 2680 2690 2700
VVPVPPTDKP STTTPAALKQ SDASKPPTAA IQHQQKLGTP VTPKDSGDKP
2710 2720 2730 2740 2750
TDNASAPVGV SPVTPDGTPG AKPPPKDAPP VDDTKQPVRK SLPSQVRGGR
2760 2770 2780 2790 2800
PYIRPSLGPF KFTGPPGYTI PVHGLPPSDS NVTQSTKEPP KPAVETPAAA
2810 2820 2830 2840 2850
PAKSAAAPAA APAKSAAAPA AAPAKSAAAP AAAPAKSAAA PAAAPAKSAA
2860 2870 2880 2890 2900
APAAAPAKDQ TKSAAEVPKP AKDQAKDQAK DQAKDQAKDQ AKDQAKSTTG
2910 2920 2930 2940 2950
QKLAKDPKSD GLTDDVALEI VPEKTPLPDD SPIGAVPENT PLPDDSPIGS
2960 2970 2980 2990 3000
PDLSASKNSH TTDAVSSDRF SVACKVPLPD SPEDDFYSYA VDVPLPDSPT
3010 3020 3030 3040 3050
DDPSSGRSDA RAPTVGGVAS IHRKSDSRNN RQSDAWRRAF ADTLHGRPRN
3060 3070 3080 3090 3100
RSATKPCKSA PYKVPHAISY TKIPSVPNDQ SGLAGKPCSE EPKRPTGRDT
3110 3120 3130 3140 3150
PVGSWNVSPS QAPADIPTAI PQNQNTSESP RTTSLKSPTR TVQSSMPADD
3160 3170 3180 3190 3200
IDELAEYDLQ IARAVPVTKH PQPPPANQTP PPQEPPAPID DRKNIRPPLS
3210 3220 3230 3240 3250
EEEIIAFLIN MDDDDAGNAS GPVDLHSVQA PKLPKQSKPT TNQFVPLDWW
3260 3270 3280 3290 3300
TETEPVVDAD SLDLSPKQQR LFSWESTRDL LNINVRDRVY EEESDDEYTV
3310 3320 3330 3340 3350
SWDQHLVPAV SPTSVSSYSS DTVTDSYTDI NDPRSVVCPL DGNAQNNVRE
3360 3370 3380 3390 3400
FLDTHSSRVR VVPADELLSR RYFRSTSLSA MALLIAACRT IVRRLRATRR
3410 3420
VLTDINRSLL LDLKQIRVLL G
Length:3,421
Mass (Da):367,083
Last modified:December 1, 1992 - v1
Checksum:i5075EFE4739BB7AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY665713 Genomic DNA. Translation: AAT67281.1.
PIRiG36797. WZBEB6.
RefSeqiYP_053069.1. NC_001491.2.

Genome annotation databases

GeneIDi1487490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY665713 Genomic DNA. Translation: AAT67281.1 .
PIRi G36797. WZBEB6.
RefSeqi YP_053069.1. NC_001491.2.

3D structure databases

ProteinModelPortali P28955.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1487490.

Family and domain databases

InterProi IPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_EHV1B
AccessioniPrimary (citable) accession number: P28955
Secondary accession number(s): Q6DLI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3