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P28908 (TNR8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 151. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tumor necrosis factor receptor superfamily member 8
Alternative name(s):
CD30L receptor
Ki-1 antigen
Lymphocyte activation antigen CD30
CD_antigen=CD30
Gene names
Name:TNFRSF8
Synonyms:CD30, D1S166E
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

Subunit structure

Interacts with TRAF1, TRAF2, TRAF3 and TRAF5. Ref.8 Ref.9 Ref.10 Ref.11 Ref.14

Subcellular location

Isoform Long: Cell membrane; Single-pass type I membrane protein.

Isoform Short: Cytoplasm.

Post-translational modification

Phosphorylated on serine and tyrosine residues.

Miscellaneous

Most specific Hodgkin disease associated antigen.

Sequence similarities

Contains 6 TNFR-Cys repeats.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Long (identifier: P28908-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P28908-2)

Also known as: Cytoplasmic; C30V;

The sequence of this isoform differs from the canonical sequence as follows:
     1-463: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818
Chain19 – 595577Tumor necrosis factor receptor superfamily member 8
PRO_0000034573

Regions

Topological domain19 – 379361Extracellular Potential
Transmembrane380 – 40728Helical; Potential
Topological domain408 – 595188Cytoplasmic Potential
Repeat28 – 6639TNFR-Cys 1
Repeat68 – 10639TNFR-Cys 2
Repeat107 – 15044TNFR-Cys 3
Repeat205 – 24137TNFR-Cys 4
Repeat243 – 28139TNFR-Cys 5
Repeat282 – 32544TNFR-Cys 6
Compositional bias347 – 37731Pro/Ser/Thr-rich

Amino acid modifications

Modified residue4521Phosphoserine Ref.12 Ref.13
Glycosylation1011N-linked (GlcNAc...) Potential
Glycosylation2761N-linked (GlcNAc...) Potential
Disulfide bond29 ↔ 44 By similarity
Disulfide bond45 ↔ 58 By similarity
Disulfide bond48 ↔ 65 By similarity
Disulfide bond69 ↔ 81 By similarity
Disulfide bond84 ↔ 98 By similarity
Disulfide bond87 ↔ 106 By similarity
Disulfide bond108 ↔ 122 By similarity
Disulfide bond131 ↔ 149 By similarity
Disulfide bond233 ↔ 240 By similarity
Disulfide bond244 ↔ 256 By similarity
Disulfide bond259 ↔ 273 By similarity
Disulfide bond262 ↔ 281 By similarity
Disulfide bond283 ↔ 297 By similarity
Disulfide bond289 ↔ 300 By similarity

Natural variations

Alternative sequence1 – 463463Missing in isoform Short.
VSP_018900
Natural variant2731C → F.
Corresponds to variant rs2230624 [ dbSNP | Ensembl ].
VAR_054213
Natural variant2731C → Y. Ref.5
VAR_018753
Natural variant2971C → R. Ref.6
Corresponds to variant rs1763642 [ dbSNP | Ensembl ].
VAR_055257
Natural variant3141P → S.
Corresponds to variant rs2275170 [ dbSNP | Ensembl ].
VAR_055258
Natural variant4021S → G. Ref.5
Corresponds to variant rs2230625 [ dbSNP | Ensembl ].
VAR_018754
Natural variant4661Q → R.
Corresponds to variant rs35511003 [ dbSNP | Ensembl ].
VAR_055259

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 7A407CC78A6E0BC8

FASTA59563,747
        10         20         30         40         50         60 
MRVLLAALGL LFLGALRAFP QDRPFEDTCH GNPSHYYDKA VRRCCYRCPM GLFPTQQCPQ 

        70         80         90        100        110        120 
RPTDCRKQCE PDYYLDEADR CTACVTCSRD DLVEKTPCAW NSSRVCECRP GMFCSTSAVN 

       130        140        150        160        170        180 
SCARCFFHSV CPAGMIVKFP GTAQKNTVCE PASPGVSPAC ASPENCKEPS SGTIPQAKPT 

       190        200        210        220        230        240 
PVSPATSSAS TMPVRGGTRL AQEAASKLTR APDSPSSVGR PSSDPGLSPT QPCPEGSGDC 

       250        260        270        280        290        300 
RKQCEPDYYL DEAGRCTACV SCSRDDLVEK TPCAWNSSRT CECRPGMICA TSATNSCARC 

       310        320        330        340        350        360 
VPYPICAAET VTKPQDMAEK DTTFEAPPLG TQPDCNPTPE NGEAPASTSP TQSLLVDSQA 

       370        380        390        400        410        420 
SKTLPIPTSA PVALSSTGKP VLDAGPVLFW VILVLVVVVG SSAFLLCHRR ACRKRIRQKL 

       430        440        450        460        470        480 
HLCYPVQTSQ PKLELVDSRP RRSSTQLRSG ASVTEPVAEE RGLMSQPLME TCHSVGAAYL 

       490        500        510        520        530        540 
ESLPLQDASP AGGPSSPRDL PEPRVSTEHT NNKIEKIYIM KADTVIVGTV KAELPEGRGL 

       550        560        570        580        590 
AGPAEPELEE ELEADHTPHY PEQETEPPLG SCSDVMLSVE EEGKEDPLPT AASGK 

« Hide

Isoform Short (Cytoplasmic) (C30V) [UniParc].

Checksum: 211648B504B6313F
Show »

FASTA13214,088

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of a new member of the nerve growth factor receptor family that is characteristic for Hodgkin's disease."
Duerkop H., Latza U., Hummel M., Eitelbach F., Seed B., Stein H.
Cell 68:421-427(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
Tissue: Lymphoid tissue.
[2]"Opposite effects of the CD30 ligand are not due to CD30 mutations: results from cDNA cloning and sequence comparison of the CD30 antigen from different sources."
Jung W., Krueger S., Renner C., Gause A., Sahin U., Trumper L., Pfreundschuh M.
Mol. Immunol. 31:1329-1334(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[3]"A variant CD30 protein lacking extracellular and transmembrane domains is induced in HL-60 by tetradecanoylphorbol acetate and is expressed in alveolar macrophages."
Horie R., Ito K., Tatewaki M., Nagai M., Aizawa S., Higashihara M., Ishida T., Inoue J., Takizawa H., Watanabe T.
Blood 88:2422-2432(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[4]"Structure of the Hodgkin's disease associated human CD30 gene and the influence of a microsatellite region on its expression in CD30+ cell lines."
Durkop H., Oberbarnscheidt M., Latza U., Bulfone-Paus S.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM LONG).
Tissue: Placenta.
[5]NIEHS SNPs program
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TYR-273 AND GLY-402.
[6]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-297.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Blood.
[8]"T cell receptor-dependent cell death of T cell hybridomas mediated by the CD30 cytoplasmic domain in association with tumor necrosis factor receptor-associated factors."
Lee S.Y., Park C.G., Choi Y.
J. Exp. Med. 183:669-674(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRAF1 AND TRAF2.
[9]"Binding sites of cytoplasmic effectors TRAF1, 2, and 3 on CD30 and other members of the TNF receptor superfamily."
Boucher L.-M., Marengere L.E., Lu Y., Thukral S., Mak T.W.
Biochem. Biophys. Res. Commun. 233:592-600(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRAF3.
[10]"Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5)."
Mizushima S., Fujita M., Ishida T., Azuma S., Kato K., Hirai M., Otsuka M., Yamamoto T., Inoue J.
Gene 207:135-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRAF5.
[11]"Tumor necrosis factor receptor-associated factor (TRAF) 5 and TRAF2 are involved in CD30-mediated NFkappaB activation."
Aizawa S., Nakano H., Ishida T., Horie R., Nagai M., Ito K., Yagita H., Okumura K., Inoue J., Watanabe T.
J. Biol. Chem. 272:2042-2045(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRAF5.
[12]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-452, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[13]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-452, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"The structural basis for the recognition of diverse receptor sequences by TRAF2."
Ye H., Park Y.C., Kreishman M., Kieff E., Wu H.
Mol. Cell 4:321-330(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 576-583 IN COMPLEX WITH TRAF2, INTERACTION WITH TRAF2.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M83554 mRNA. Translation: AAA51947.1.
S75768 mRNA. Translation: AAD14188.1.
D86042 mRNA. Translation: BAA12973.1.
AJ289159 Genomic DNA. Translation: CAC16652.1.
AY498860 Genomic DNA. Translation: AAR32099.1.
AL357835 Genomic DNA. Translation: CAH73719.1.
BC063482 mRNA. Translation: AAH63482.2.
PIRA42086.
RefSeqNP_001234.2. NM_001243.3.
NP_001268359.1. NM_001281430.1.
UniGeneHs.1314.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D01X-ray2.00G/H/I576-583[»]
ProteinModelPortalP28908.
SMRP28908. Positions 42-156, 233-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107381. 11 interactions.
DIPDIP-2930N.
IntActP28908. 3 interactions.
STRING9606.ENSP00000263932.

Chemistry

ChEMBLCHEMBL2364161.
GuidetoPHARMACOLOGY1877.

PTM databases

PhosphoSiteP28908.

Polymorphism databases

DMDM115978.

Proteomic databases

PaxDbP28908.
PRIDEP28908.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000413146; ENSP00000398337; ENSG00000120949. [P28908-2]
GeneID943.
KEGGhsa:943.
UCSCuc001atq.3. human. [P28908-1]
uc001atr.3. human. [P28908-2]

Organism-specific databases

CTD943.
GeneCardsGC01P012123.
H-InvDBHIX0200047.
HGNCHGNC:11923. TNFRSF8.
HPACAB000016.
MIM153243. gene.
neXtProtNX_P28908.
PharmGKBPA36616.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45191.
HOGENOMHOG000220871.
HOVERGENHBG005054.
InParanoidP28908.
KOK05145.
OrthoDBEOG786H2Q.
PhylomeDBP28908.
TreeFamTF331157.

Enzyme and pathway databases

SignaLinkP28908.

Gene expression databases

ArrayExpressP28908.
BgeeP28908.
CleanExHS_TNFRSF8.
GenevestigatorP28908.

Family and domain databases

InterProIPR001368. TNFR/NGFR_Cys_rich_reg.
IPR020416. TNFR_8.
[Graphical view]
PfamPF00020. TNFR_c6. 3 hits.
[Graphical view]
PRINTSPR01923. TNFACTORR8.
SMARTSM00208. TNFR. 4 hits.
[Graphical view]
PROSITEPS00652. TNFR_NGFR_1. 2 hits.
PS50050. TNFR_NGFR_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP28908.
GeneWikiCD30.
GenomeRNAi943.
NextBio3908.
PROP28908.
SOURCESearch...

Entry information

Entry nameTNR8_HUMAN
AccessionPrimary (citable) accession number: P28908
Secondary accession number(s): B1AN79, Q6P4D9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries