ID   CD34_HUMAN              Reviewed;         385 AA.
AC   P28906; A8K664; Q15970; Q15971; Q5JTA4; Q9UJB1;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   07-JUL-2009, entry version 85.
DE   RecName: Full=Hematopoietic progenitor cell antigen CD34;
DE   AltName: CD_antigen=CD34;
DE   Flags: Precursor;
GN   Name=CD34;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CD34-F), AND PROTEIN SEQUENCE OF
RP   32-43.
RC   TISSUE=Bone marrow;
RX   MEDLINE=92091783; PubMed=1370171;
RA   Simmons D.L., Satterthwaite A.B., Tenen D.G., Seed B.;
RT   "Molecular cloning of a cDNA encoding CD34, a sialomucin of human
RT   hematopoietic stem cells.";
RL   J. Immunol. 148:267-271(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM CD34-F).
RX   MEDLINE=92241880; PubMed=1374051; DOI=10.1016/0888-7543(92)90310-O;
RA   Satterthwaite A.B., Burn T.C., le Beau M.M., Tenen D.G.;
RT   "Structure of the gene encoding CD34, a human hematopoietic stem cell
RT   antigen.";
RL   Genomics 12:788-794(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CD34-F AND CD34-T).
RC   TISSUE=Megakaryoblast;
RX   MEDLINE=93146100; PubMed=7678811;
RA   Nakamura Y., Komano H., Nakauchi H.;
RT   "Two alternative forms of cDNA encoding CD34.";
RL   Exp. Hematol. 21:236-242(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CD34-F).
RC   TISSUE=Heart;
RA   Freund D., Wiebe G.J., Ehninger G., Corbeil D.;
RT   "Sequence analysis of the human CD34 antigen.";
RL   Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CD34-F).
RC   TISSUE=Placenta;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA   Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA   Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA   McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA   Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA   Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA   Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA   Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA   Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA   Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA   Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA   Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA   Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA   Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA   Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA   Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA   Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA   Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA   Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA   Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA   Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA   Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA   Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA   Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA   Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CD34-F).
RC   TISSUE=Skin;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   PROTEIN SEQUENCE OF 32-47 AND 175-191, AND CHARACTERIZATION.
RX   MEDLINE=89069662; PubMed=2462139;
RA   Sutherland D.R., Watt S.M., Dowden G., Karhi K., Baker M.A.,
RA   Greaves M.F., Smart J.E.;
RT   "Structural and partial amino acid sequence analysis of the human
RT   hemopoietic progenitor cell antigen CD34.";
RL   Leukemia 2:793-803(1988).
RN   [9]
RP   PHOSPHORYLATION BY PKC.
RX   MEDLINE=90293044; PubMed=1694174;
RA   Fackler M.J., Civin C.I., Sutherland D.R., Baker M.A., May W.S.;
RT   "Activated protein kinase C directly phosphorylates the CD34 antigen
RT   on hematopoietic cells.";
RL   J. Biol. Chem. 265:11056-11061(1990).
CC   -!- FUNCTION: Possible adhesion molecule with a role in early
CC       hematopoiesis by mediating the attachment of stem cells to the
CC       bone marrow extracellular matrix or directly to stromal cells.
CC       Could act as a scaffold for the attachment of lineage specific
CC       glycans, allowing stem cells to bind to lectins expressed by
CC       stromal cells or other marrow components. Presents carbohydrate
CC       ligands to selectins.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane
CC       protein.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC         Comment=Both isoforms are expressed on the cell surface.
CC         CD34-T/CD34-F ratio increases with cell differentiation;
CC       Name=CD34-F;
CC         IsoId=P28906-1; Sequence=Displayed;
CC       Name=CD34-T;
CC         IsoId=P28906-2; Sequence=VSP_004159, VSP_004160;
CC   -!- TISSUE SPECIFICITY: Selectively expressed on hematopoietic
CC       progenitor cells and the small vessel endothelium of a variety of
CC       tissues.
CC   -!- DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
CC   -!- PTM: Highly glycosylated.
CC   -!- PTM: Phosphorylated on serine residues by PKC.
CC   -!- DISEASE: Abnormal CD34 expression in leukemogenesis.
CC   -!- SIMILARITY: Belongs to the CD34 family.
CC   -!- WEB RESOURCE: Name=Wikipedia; Note=CD34 entry;
CC       URL="http://en.wikipedia.org/wiki/CD34";
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DR   EMBL; M81104; AAA03181.1; ALT_INIT; mRNA.
DR   EMBL; M81945; AAA03659.1; -; Genomic_DNA.
DR   EMBL; M81938; AAA03659.1; JOINED; Genomic_DNA.
DR   EMBL; M81939; AAA03659.1; JOINED; Genomic_DNA.
DR   EMBL; M81940; AAA03659.1; JOINED; Genomic_DNA.
DR   EMBL; M81941; AAA03659.1; JOINED; Genomic_DNA.
DR   EMBL; M81942; AAA03659.1; JOINED; Genomic_DNA.
DR   EMBL; M81943; AAA03659.1; JOINED; Genomic_DNA.
DR   EMBL; M81944; AAA03659.1; JOINED; Genomic_DNA.
DR   EMBL; S53910; AAB25222.1; -; mRNA.
DR   EMBL; S53911; AAB25223.1; -; mRNA.
DR   EMBL; AF523361; AAM82157.1; -; mRNA.
DR   EMBL; AK291529; BAF84218.1; -; mRNA.
DR   EMBL; AL356275; CAI40195.1; -; Genomic_DNA.
DR   EMBL; BC039146; AAH39146.2; -; mRNA.
DR   IPI; IPI00006070; -.
DR   IPI; IPI00299994; -.
DR   PIR; A38078; A38078.
DR   PIR; I67604; I67604.
DR   RefSeq; NP_001020280.1; -.
DR   RefSeq; NP_001764.1; -.
DR   UniGene; Hs.374990; -.
DR   PhosphoSite; P28906; -.
DR   PRIDE; P28906; -.
DR   Ensembl; ENSG00000174059; Homo sapiens.
DR   GeneID; 947; -.
DR   KEGG; hsa:947; -.
DR   UCSC; uc001hgw.1; human.
DR   GeneCards; GC01M206116; -.
DR   H-InvDB; HIX0001543; -.
DR   HGNC; HGNC:1662; CD34.
DR   HPA; CAB000018; -.
DR   MIM; 142230; gene.
DR   PharmGKB; PA26211; -.
DR   HOGENOM; P28906; -.
DR   HOVERGEN; P28906; -.
DR   OMA; P28906; HQSDLRK.
DR   Reactome; REACT_6900; Signaling in Immune system.
DR   NextBio; 3932; -.
DR   ArrayExpress; P28906; -.
DR   Bgee; P28906; -.
DR   CleanEx; HS_CD34; -.
DR   GermOnline; ENSG00000174059; Homo sapiens.
DR   GO; GO:0009897; C:external side of plasma membrane; IC:HGNC.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030246; F:carbohydrate binding; IDA:HGNC.
DR   GO; GO:0005515; F:protein binding; IEA:UniProtKB-KW.
DR   GO; GO:0016337; P:cell-cell adhesion; IDA:HGNC.
DR   GO; GO:0050900; P:leukocyte migration; ISS:HGNC.
DR   InterPro; IPR008083; CD34.
DR   InterPro; IPR013836; CD34/Podocalyxin.
DR   PANTHER; PTHR16677; CD34; 1.
DR   Pfam; PF06365; CD34_antigen; 1.
DR   PRINTS; PR01700; CD34ANTIGEN.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell adhesion; Complete proteome;
KW   Direct protein sequencing; Glycoprotein; Membrane; Phosphoprotein;
KW   Polymorphism; Signal; Transmembrane.
FT   SIGNAL        1     31
FT   CHAIN        32    385       Hematopoietic progenitor cell antigen
FT                                CD34.
FT                                /FTId=PRO_0000020900.
FT   TOPO_DOM     32    290       Extracellular (Potential).
FT   TRANSMEM    291    311       Potential.
FT   TOPO_DOM    312    385       Cytoplasmic (Potential).
FT   MOD_RES     329    329       Phosphotyrosine (By similarity).
FT   MOD_RES     339    339       Phosphotyrosine (By similarity).
FT   CARBOHYD     36     36       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD     51     51       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD     55     55       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD     85     85       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    107    107       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    126    126       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    142    142       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    194    194       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    249    249       N-linked (GlcNAc...) (Potential).
FT   VAR_SEQ     325    328       GEDP -> ELEP (in isoform CD34-T).
FT                                /FTId=VSP_004159.
FT   VAR_SEQ     329    385       Missing (in isoform CD34-T).
FT                                /FTId=VSP_004160.
FT   VARIANT     367    367       A -> S (in dbSNP:rs28362497).
FT                                /FTId=VAR_050774.
FT   CONFLICT    187    187       Q -> E (in Ref. 8; AA sequence).
FT   CONFLICT    325    327       GED -> ELE (in Ref. 3).
FT   CONFLICT    361    361       E -> K (in Ref. 1 and 2).
SQ   SEQUENCE   385 AA;  40716 MW;  BC581A8FCFFE2BB3 CRC64;
     MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS NVSTNVSYQE
     TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV
     FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI
     REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP
     QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL
     LAVLGITGYF LMNRRSWSPT GERLGEDPYY TENGGGQGYS SGPGTSPEAQ GKASVNRGAQ
     ENGTGQATSR NGHSARQHVV ADTEL
//