ID   CD34_HUMAN     STANDARD;      PRT;   385 AA.
AC   P28906; Q15970; Q15971;
DT   01-DEC-1992 (Rel. 24, Created)
DT   15-JUL-1998 (Rel. 36, Last sequence update)
DT   01-OCT-2000 (Rel. 40, Last annotation update)
DE   HEMATOPOIETIC PROGENITOR CELL ANTIGEN CD34 PRECURSOR.
GN   CD34.
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   SEQUENCE FROM N.A., AND SEQUENCE OF 32-43.
RC   TISSUE=BONE MARROW;
RX   MEDLINE=92091783; PubMed=1370171;
RA   Simmons D.L., Satterthwaite A.B., Tenen D.G., Seed B.;
RT   "Molecular cloning of a cDNA encoding CD34, a sialomucin of human
RT   hematopoietic stem cells.";
RL   J. Immunol. 148:267-271(1992).
RN   [2]
RP   SEQUENCE FROM N.A.
RX   MEDLINE=92241880; PubMed=1374051;
RA   Satterthwaite A.B., Burn T.C., le Beau M.M., Tenen D.G.;
RT   "Structure of the gene encoding CD34, a human hematopoietic stem cell
RT   antigen.";
RL   Genomics 12:788-794(1992).
RN   [3]
RP   SEQUENCE FROM N.A. (CD34-F AND CD34-T).
RC   TISSUE=MEGAKARYOBLAST;
RX   MEDLINE=93146100; PubMed=7678811;
RA   Nakamura Y., Komano H., Nakauchi H.;
RT   "Two alternative forms of cDNA encoding CD34.";
RL   Exp. Hematol. 21:236-242(1993).
RN   [4]
RP   SEQUENCE OF 32-47, AND 175-191, AND CHARACTERIZATION.
RX   MEDLINE=89069662; PubMed=2462139;
RA   Sutherland D.R., Watt S.M., Dowden G., Karhi K., Baker M.A.,
RA   Greaves M.F., Smart J.E.;
RT   "Structural and partial amino acid sequence analysis of the human
RT   hemopoietic progenitor cell antigen CD34.";
RL   Leukemia 2:793-803(1988).
RN   [5]
RP   PHOSPHORYLATION BY PKC.
RX   MEDLINE=90293044; PubMed=1694174;
RA   Fackler M.J., Civin C.I., Sutherland D.R., Baker M.A., May W.S.;
RT   "Activated protein kinase C directly phosphorylates the CD34 antigen
RT   on hematopoietic cells.";
RL   J. Biol. Chem. 265:11056-11061(1990).
CC   -!- FUNCTION: POSSIBLE ADHESION MOLECULE WITH A ROLE IN EARLY
CC       HEMATOPOIESIS BY MEDIATING THE ATTACHMENT OF STEM CELLS TO THE
CC       BONE MARROW EXTRACELLULAR MATRIX OR DIRECTLY TO STROMAL CELLS.
CC       COULD ACT AS A SCAFFOLD FOR THE ATTACHMENT OF LINEAGE SPECIFIC
CC       GLYCANS, ALLOWING STEM CELLS TO BIND TO LECTINS EXPRESSED BY
CC       STROMAL CELLS OR OTHER MARROW COMPONENTS. PRESENTS CARBOHYDRATE
CC       LIGANDS TO SELECTINS.
CC   -!- SUBCELLULAR LOCATION: TYPE I MEMBRANE PROTEIN.
CC   -!- ALTERNATIVE PRODUCTS: 2 ISOFORMS; CD34-F (SHOWN HERE) AND CD34-T;
CC       ARE PRODUCED BY ALTERNATIVE SPLICING. BOTH ISOFORMS ARE EXPRESSED
CC       ON THE CELL SURFACE. CD34-T/CD34-F RATIO INCREASES WITH CELL
CC       DIFFERENTIATION.
CC   -!- TISSUE SPECIFICITY: SELECTIVELY EXPRESSED ON HEMATOPOIETIC
CC       PROGENITOR CELLS AND THE SMALL VESSEL ENDOTHELIUM OF A VARIETY OF
CC       TISSUES.
CC   -!- DEVELOPMENTAL STAGE: ON EARLY HEMATOPOIETIC PROGENITOR CELLS.
CC   -!- PTM: EXTENSIVELY O-GLYCOSYLATED AND N-GLYCOSYLATED. PHOSPHORYLATED
CC       ON SERINE RESIDUES BY PKC.
CC   -!- DISEASE: ABNORMAL CD34 EXPRESSION IN LEUKEMOGENESIS.
CC   -!- DATABASE: NAME=PROW; NOTE=CD guide CD34 entry;
CC       WWW="http://www.ncbi.nlm.nih.gov/prow/cd/cd34.htm".
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DR   EMBL; M81104; AAA03181.1; ALT_INIT.
DR   EMBL; M81945; AAA03659.1; -.
DR   EMBL; M81938; AAA03659.1; JOINED.
DR   EMBL; M81939; AAA03659.1; JOINED.
DR   EMBL; M81940; AAA03659.1; JOINED.
DR   EMBL; M81941; AAA03659.1; JOINED.
DR   EMBL; M81942; AAA03659.1; JOINED.
DR   EMBL; M81943; AAA03659.1; JOINED.
DR   EMBL; M81944; AAA03659.1; JOINED.
DR   EMBL; S53910; AAB25222.1; -.
DR   EMBL; S53911; AAB25223.1; -.
DR   PIR; S28534; S28534.
DR   PIR; A46487; A46487.
DR   MIM; 142230; -.
KW   Glycoprotein; Transmembrane; Signal; Antigen; Alternative splicing;
KW   Phosphorylation.
FT   SIGNAL        1     31
FT   CHAIN        32    385       HEMATOPOIETIC PROGENITOR CELL ANTIGEN
FT                                CD34.
FT   DOMAIN       32    290       EXTRACELLULAR (POTENTIAL).
FT   TRANSMEM    291    311       POTENTIAL.
FT   DOMAIN      312    385       CYTOPLASMIC (POTENTIAL).
FT   CARBOHYD     36     36       N-LINKED (GLCNAC...) (POTENTIAL).
FT   CARBOHYD     51     51       N-LINKED (GLCNAC...) (POTENTIAL).
FT   CARBOHYD     55     55       N-LINKED (GLCNAC...) (POTENTIAL).
FT   CARBOHYD     85     85       N-LINKED (GLCNAC...) (POTENTIAL).
FT   CARBOHYD    107    107       N-LINKED (GLCNAC...) (POTENTIAL).
FT   CARBOHYD    126    126       N-LINKED (GLCNAC...) (POTENTIAL).
FT   CARBOHYD    142    142       N-LINKED (GLCNAC...) (POTENTIAL).
FT   CARBOHYD    194    194       N-LINKED (GLCNAC...) (POTENTIAL).
FT   CARBOHYD    249    249       N-LINKED (GLCNAC...) (POTENTIAL).
FT   VARSPLIC    325    328       GEDP -> ELEP (IN ISOFORM CD34-T).
FT   VARSPLIC    329    385       MISSING (IN ISOFORM CD34-T).
FT   CONFLICT    187    187       Q -> E (IN REF. 4).
FT   CONFLICT    361    361       E -> K (IN REF. 1 AND 2).
SQ   SEQUENCE   385 AA;  40716 MW;  BC581A8FCFFE2BB3 CRC64;
     MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS NVSTNVSYQE
     TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV
     FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI
     REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP
     QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL
     LAVLGITGYF LMNRRSWSPT GERLGEDPYY TENGGGQGYS SGPGTSPEAQ GKASVNRGAQ
     ENGTGQATSR NGHSARQHVV ADTEL
//