P28906 (CD34_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hematopoietic progenitor cell antigen CD34

Alternative name(s):
CD_antigen=CD34

Gene names

Name:

CD34

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Developmental stage	On early hematopoietic progenitor cells.
Post-translational modification	Highly glycosylated. Phosphorylated on serine residues by PKC. Ref.10
Sequence similarities	Belongs to the CD34 family.
Sequence caution	The sequence AAA03181.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell adhesion Inferred from direct assay PubMed 15249540. Source: HGNC endothelial cell proliferation Inferred from direct assay PubMed 21835908. Source: UniProtKB endothelium development Inferred from expression pattern PubMed 17261663. Source: UniProtKB extracellular vesicular exosome assembly Inferred from direct assay PubMed 21835908. Source: UniProtKB glomerular endothelium development Inferred from expression pattern PubMed 17464107. Source: UniProtKB glomerular filtration Inferred from expression pattern PubMed 20354148. Source: UniProtKB glutamate metabolic process Inferred from direct assay PubMed 17505307. Source: UniProtKB hematopoietic stem cell proliferation Inferred from mutant phenotype PubMed 21873977. Source: UniProtKB leukocyte migration Inferred from sequence or structural similarity. Source: HGNC mesangial cell-matrix adhesion Inferred from sequence or structural similarity. Source: UniProtKB metanephric glomerular mesangial cell differentiation Inferred from expression pattern PubMed 1868864. Source: UniProtKB negative regulation of blood coagulation Inferred from direct assay PubMed 17505307. Source: UniProtKB negative regulation of cellular response to heat Inferred from direct assay PubMed 17505307. Source: UniProtKB negative regulation of cellular response to hypoxia Inferred from direct assay PubMed 17505307. Source: UniProtKB negative regulation of gene expression Inferred from mutant phenotype PubMed 15969628. Source: UniProtKB negative regulation of interleukin-2 secretion Inferred from mutant phenotype PubMed 9815891. Source: UniProtKB negative regulation of necrotic cell death Inferred from direct assay PubMed 17505307. Source: UniProtKB negative regulation of nitric oxide biosynthetic process Inferred from direct assay PubMed 17505307. Source: UniProtKB negative regulation of tumor necrosis factor production Inferred from direct assay PubMed 17505307. Source: UniProtKB paracrine signaling Inferred from direct assay PubMed 21835908. Source: UniProtKB positive regulation of angiogenesis Inferred from direct assay PubMed 12939361PubMed 21835908. Source: UniProtKB positive regulation of gene expression Inferred from expression pattern PubMed 17261663. Source: UniProtKB positive regulation of glial cell line-derived neurotrophic factor secretion Inferred from direct assay PubMed 17505307. Source: UniProtKB positive regulation of granulocyte colony-stimulating factor production Inferred from direct assay PubMed 9815891. Source: UniProtKB positive regulation of interleukin-10 production Inferred from direct assay PubMed 17505307. Source: UniProtKB positive regulation of odontogenesis Inferred from direct assay PubMed 12939361. Source: UniProtKB positive regulation of transforming growth factor beta production Inferred from direct assay PubMed 17505307. Source: UniProtKB positive regulation of vasculogenesis Inferred from direct assay PubMed 12939361. Source: UniProtKB regulation of blood pressure Inferred from direct assay PubMed 17505307. Source: UniProtKB regulation of immune response Traceable author statement. Source: Reactome signal transduction Inferred from expression pattern PubMed 17261663. Source: UniProtKB stem cell proliferation Inferred from expression pattern PubMed 17261663. Source: UniProtKB tissue homeostasis Inferred from direct assay PubMed 12939361. Source: UniProtKB transdifferentiation Inferred from expression pattern PubMed 1868864. Source: UniProtKB vascular wound healing Inferred from expression pattern PubMed 20354148. Source: UniProtKB
Cellular_component	apical plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB basal plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB external side of plasma membrane Inferred from direct assay PubMed 10337918PubMed 12939361PubMed 20354148. Source: UniProtKB glomerular endothelium fenestra Inferred from sequence or structural similarity. Source: UniProtKB integral to plasma membrane Traceable author statement PubMed 9042286. Source: UniProtKB intercellular bridge Inferred from direct assay PubMed 7525669. Source: UniProtKB lysosome Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay PubMed 7525669. Source: UniProtKB
Molecular_function	carbohydrate binding Inferred from direct assay PubMed 15249540. Source: HGNC sulfate binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Both isoforms are expressed on the cell surface. CD34-T/CD34-F ratio increases with cell differentiation.

Isoform CD34-F (identifier: P28906-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CD34-T (identifier: P28906-2)

The sequence of this isoform differs from the canonical sequence as follows:
     325-328: GEDP → ELEP
     329-385: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Ref.1 Ref.9
	Chain	32 – 385	354	Hematopoietic progenitor cell antigen CD34		PRO_0000020900
Regions
	Topological domain	32 – 290	259	Extracellular Potential
	Transmembrane	291 – 311	21	Helical; Potential
	Topological domain	312 – 385	74	Cytoplasmic Potential
Amino acid modifications
	Modified residue	329	1	Phosphotyrosine By similarity
	Modified residue	339	1	Phosphotyrosine By similarity
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	194	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	325 – 328	4	GEDP → ELEP in isoform CD34-T.		VSP_004159
	Alternative sequence	329 – 385	57	Missing in isoform CD34-T.		VSP_004160
	Natural variant	367	1	A → S. Corresponds to variant rs28362497 [ dbSNP | Ensembl ].		VAR_050774
Experimental info
	Sequence conflict	187	1	Q → E AA sequence Ref.9
	Sequence conflict	325 – 327	3	GED → ELE Ref.3
	Sequence conflict	361	1	E → K Ref.1
	Sequence conflict	361	1	E → K Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform CD34-F [UniParc]. Last modified July 15, 1998. Version 2. Checksum: BC581A8FCFFE2BB3	FASTA	385	40,716
	10 20 30 40 50 60 MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS NVSTNVSYQE 70 80 90 100 110 120 TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV 130 140 150 160 170 180 FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI 190 200 210 220 230 240 REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP 250 260 270 280 290 300 QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL 310 320 330 340 350 360 LAVLGITGYF LMNRRSWSPT GERLGEDPYY TENGGGQGYS SGPGTSPEAQ GKASVNRGAQ 370 380 ENGTGQATSR NGHSARQHVV ADTEL
	Isoform CD34-T [UniParc]. Checksum: 7FFBEBC98D4CC61D	FASTA	328	35,011
	10 20 30 40 50 60 MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS NVSTNVSYQE 70 80 90 100 110 120 TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV 130 140 150 160 170 180 FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI 190 200 210 220 230 240 REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP 250 260 270 280 290 300 QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL 310 320 LAVLGITGYF LMNRRSWSPT GERLELEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells." Simmons D.L., Satterthwaite A.B., Tenen D.G., Seed B. J. Immunol. 148:267-271(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CD34-F), PROTEIN SEQUENCE OF 32-43. Tissue: Bone marrow.
[2]	"Structure of the gene encoding CD34, a human hematopoietic stem cell antigen." Satterthwaite A.B., Burn T.C., le Beau M.M., Tenen D.G. Genomics 12:788-794(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM CD34-F).
[3]	"Two alternative forms of cDNA encoding CD34." Nakamura Y., Komano H., Nakauchi H. Exp. Hematol. 21:236-242(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CD34-F AND CD34-T). Tissue: Megakaryoblast.
[4]	"Sequence analysis of the human CD34 antigen." Freund D., Wiebe G.J., Ehninger G., Corbeil D. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CD34-F). Tissue: Heart.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CD34-F). Tissue: Placenta.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CD34-F). Tissue: Skin.
[9]	"Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34." Sutherland D.R., Watt S.M., Dowden G., Karhi K., Baker M.A., Greaves M.F., Smart J.E. Leukemia 2:793-803(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-47 AND 175-191, CHARACTERIZATION.
[10]	"Activated protein kinase C directly phosphorylates the CD34 antigen on hematopoietic cells." Fackler M.J., Civin C.I., Sutherland D.R., Baker M.A., May W.S. J. Biol. Chem. 265:11056-11061(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY PKC.
+	Additional computationally mapped references.

Web resources

Wikipedia

CD34 entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	M81104 mRNA. Translation: AAA03181.1. Different initiation. M81945 , M81938, M81939, M81940, M81941, M81942, M81943, M81944 Genomic DNA. Translation: AAA03659.1. S53910 mRNA. Translation: AAB25222.1. S53911 mRNA. Translation: AAB25223.1. AF523361 mRNA. Translation: AAM82157.1. AK291529 mRNA. Translation: BAF84218.1. AL356275 Genomic DNA. Translation: CAI40195.1. AL356275 Genomic DNA. Translation: CAI40196.1. CH471100 Genomic DNA. Translation: EAW93459.1. BC039146 mRNA. Translation: AAH39146.2.
IPI	IPI00006070. IPI00299994.
PIR	A38078. I67604.
RefSeq	NP_001020280.1. NM_001025109.1. NP_001764.1. NM_001773.2.
UniGene	Hs.374990.
3D structure databases
ProteinModelPortal	P28906.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28906. 1 interaction.
STRING	9606.ENSP00000310036.
PTM databases
PhosphoSite	P28906.
Polymorphism databases
DMDM	3183511.
Proteomic databases
PaxDb	P28906.
PRIDE	P28906.
Protocols and materials databases
DNASU	947.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000310833; ENSP00000310036; ENSG00000174059. ENST00000356522; ENSP00000348916; ENSG00000174059.
GeneID	947.
KEGG	hsa:947.
UCSC	uc001hgw.1. human. uc001hgx.1. human.
Organism-specific databases
CTD	947.
GeneCards	GC01M208057.
HGNC	HGNC:1662. CD34.
HPA	CAB000018.
MIM	142230. gene.
neXtProt	NX_P28906.
PharmGKB	PA26211.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42285.
HOVERGEN	HBG003461.
InParanoid	P28906.
KO	K06474.
OMA	GMPRGWT.
OrthoDB	EOG4GF3G0.
PhylomeDB	P28906.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	P28906.
Bgee	P28906.
CleanEx	HS_CD34.
Genevestigator	P28906.
GermOnline	ENSG00000174059. Homo sapiens.
Family and domain databases
InterPro	IPR008083. CD34. IPR013836. CD34/Podocalyxin. [Graphical view]
PANTHER	PTHR16677. PTHR16677. 1 hit.
Pfam	PF06365. CD34_antigen. 1 hit. [Graphical view]
PRINTS	PR01700. CD34ANTIGEN.
ProtoNet	Search...
Other
ChiTaRS	CD34. human.
GenomeRNAi	947.
NextBio	3932.
SOURCE	Search...

Entry information

Entry name

CD34_HUMAN

Accession

Primary (citable) accession number: P28906
Secondary accession number(s): A8K664 , Q15970, Q15971, Q5JTA3, Q5JTA4, Q9UJB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.