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Protein

Hematopoietic progenitor cell antigen CD34

Gene

CD34

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

GO - Molecular functioni

  • carbohydrate binding Source: HGNC
  • sulfate binding Source: Ensembl
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • cell-matrix adhesion Source: GO_Central
  • cell motility Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • endothelial cell proliferation Source: UniProtKB
  • endothelium development Source: UniProtKB
  • extracellular exosome assembly Source: UniProtKB
  • glomerular endothelium development Source: UniProtKB
  • glomerular filtration Source: UniProtKB
  • hematopoietic stem cell proliferation Source: UniProtKB
  • hemopoiesis Source: UniProtKB
  • leukocyte migration Source: HGNC
  • mesangial cell-matrix adhesion Source: UniProtKB
  • metanephric glomerular mesangial cell differentiation Source: UniProtKB
  • negative regulation of blood coagulation Source: UniProtKB
  • negative regulation of cellular response to heat Source: UniProtKB
  • negative regulation of cellular response to hypoxia Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of interleukin-2 secretion Source: UniProtKB
  • negative regulation of neuron death Source: UniProtKB
  • negative regulation of nitric oxide biosynthetic process Source: UniProtKB
  • negative regulation of tumor necrosis factor production Source: UniProtKB
  • paracrine signaling Source: UniProtKB
  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of glial cell-derived neurotrophic factor secretion Source: UniProtKB
  • positive regulation of granulocyte colony-stimulating factor production Source: UniProtKB
  • positive regulation of interleukin-10 production Source: UniProtKB
  • positive regulation of odontogenesis Source: UniProtKB
  • positive regulation of transforming growth factor beta production Source: UniProtKB
  • positive regulation of vasculogenesis Source: UniProtKB
  • regulation of blood pressure Source: UniProtKB
  • regulation of immune response Source: Reactome
  • signal transduction Source: UniProtKB
  • single organismal cell-cell adhesion Source: HGNC
  • stem cell proliferation Source: UniProtKB
  • tissue homeostasis Source: UniProtKB
  • transdifferentiation Source: UniProtKB
  • vascular wound healing Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

BioCyciZFISH:ENSG00000174059-MONOMER.
ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
SIGNORiP28906.

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietic progenitor cell antigen CD34
Alternative name(s):
CD_antigen: CD34
Gene namesi
Name:CD34
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:1662. CD34.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 290ExtracellularSequence analysisAdd BLAST259
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21
Topological domaini312 – 385CytoplasmicSequence analysisAdd BLAST74

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • basal plasma membrane Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • external side of plasma membrane Source: UniProtKB
  • glomerular endothelium fenestra Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • intercellular bridge Source: UniProtKB
  • lysosome Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi947.
OpenTargetsiENSG00000174059.
PharmGKBiPA26211.

Polymorphism and mutation databases

BioMutaiCD34.
DMDMi3183511.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 312 PublicationsAdd BLAST31
ChainiPRO_000002090032 – 385Hematopoietic progenitor cell antigen CD34Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...)Sequence analysis1
Glycosylationi51N-linked (GlcNAc...)Sequence analysis1
Glycosylationi55N-linked (GlcNAc...)Sequence analysis1
Glycosylationi85N-linked (GlcNAc...)Sequence analysis1
Glycosylationi107N-linked (GlcNAc...)Sequence analysis1
Glycosylationi126N-linked (GlcNAc...)Sequence analysis1
Glycosylationi142N-linked (GlcNAc...)Sequence analysis1
Glycosylationi194N-linked (GlcNAc...)Sequence analysis1
Glycosylationi249N-linked (GlcNAc...)Sequence analysis1
Modified residuei329PhosphotyrosineBy similarity1
Modified residuei339PhosphotyrosineBy similarity1
Modified residuei346PhosphoserineBy similarity1

Post-translational modificationi

Highly glycosylated.
Phosphorylated on serine residues by PKC.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP28906.
MaxQBiP28906.
PaxDbiP28906.
PeptideAtlasiP28906.
PRIDEiP28906.

PTM databases

iPTMnetiP28906.
PhosphoSitePlusiP28906.

Expressioni

Tissue specificityi

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Developmental stagei

On early hematopoietic progenitor cells.

Gene expression databases

BgeeiENSG00000174059.
CleanExiHS_CD34.
ExpressionAtlasiP28906. baseline and differential.
GenevisibleiP28906. HS.

Organism-specific databases

HPAiCAB000018.
HPA036722.
HPA036723.

Interactioni

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi107385. 1 interactor.
IntActiP28906. 1 interactor.
STRINGi9606.ENSP00000310036.

Structurei

3D structure databases

ProteinModelPortaliP28906.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CD34 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPJJ. Eukaryota.
ENOG410YPR6. LUCA.
GeneTreeiENSGT00390000008414.
HOVERGENiHBG003461.
InParanoidiP28906.
KOiK06474.
OMAiGMPRGWT.
OrthoDBiEOG091G0K1S.
PhylomeDBiP28906.
TreeFamiTF335795.

Family and domain databases

InterProiIPR008083. CD34.
IPR013836. CD34/Podocalyxin.
[Graphical view]
PANTHERiPTHR16677. PTHR16677. 1 hit.
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]
PRINTSiPR01700. CD34ANTIGEN.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Both isoforms are expressed on the cell surface. CD34-T/CD34-F ratio increases with cell differentiation.
Isoform CD34-F (identifier: P28906-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVRRGARAG PRMPRGWTAL CLLSLLPSGF MSLDNNGTAT PELPTQGTFS
60 70 80 90 100
NVSTNVSYQE TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT
110 120 130 140 150
SVYGNTNSSV QSQTSVISTV FTTPANVSTP ETTLKPSLSP GNVSDLSTTS
160 170 180 190 200
TSLATSPTKP YTSSSPILSD IKAEIKCSGI REVKLTQGIC LEQNKTSSCA
210 220 230 240 250
EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP QCLLLVLANR
260 270 280 290 300
TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT LIALVTSGAL
310 320 330 340 350
LAVLGITGYF LMNRRSWSPT GERLGEDPYY TENGGGQGYS SGPGTSPEAQ
360 370 380
GKASVNRGAQ ENGTGQATSR NGHSARQHVV ADTEL
Length:385
Mass (Da):40,716
Last modified:July 15, 1998 - v2
Checksum:iBC581A8FCFFE2BB3
GO
Isoform CD34-T (identifier: P28906-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     325-328: GEDP → ELEP
     329-385: Missing.

Show »
Length:328
Mass (Da):35,011
Checksum:i7FFBEBC98D4CC61D
GO

Sequence cautioni

The sequence AAA03181 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187Q → E AA sequence (PubMed:2462139).Curated1
Sequence conflicti325 – 327GED → ELE (PubMed:7678811).Curated3
Sequence conflicti361E → K (PubMed:1370171).Curated1
Sequence conflicti361E → K (PubMed:1374051).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050774367A → S.Corresponds to variant rs28362497dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004159325 – 328GEDP → ELEP in isoform CD34-T. 1 Publication4
Alternative sequenceiVSP_004160329 – 385Missing in isoform CD34-T. 1 PublicationAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81104 mRNA. Translation: AAA03181.1. Different initiation.
M81945
, M81938, M81939, M81940, M81941, M81942, M81943, M81944 Genomic DNA. Translation: AAA03659.1.
S53910 mRNA. Translation: AAB25222.1.
S53911 mRNA. Translation: AAB25223.1.
AF523361 mRNA. Translation: AAM82157.1.
AK291529 mRNA. Translation: BAF84218.1.
AL356275 Genomic DNA. Translation: CAI40195.1.
AL356275 Genomic DNA. Translation: CAI40196.1.
CH471100 Genomic DNA. Translation: EAW93459.1.
BC039146 mRNA. Translation: AAH39146.2.
CCDSiCCDS31011.1. [P28906-1]
CCDS31012.1. [P28906-2]
PIRiA38078.
I67604.
RefSeqiNP_001020280.1. NM_001025109.1. [P28906-1]
NP_001764.1. NM_001773.2. [P28906-2]
UniGeneiHs.374990.

Genome annotation databases

EnsembliENST00000310833; ENSP00000310036; ENSG00000174059. [P28906-1]
ENST00000356522; ENSP00000348916; ENSG00000174059. [P28906-2]
GeneIDi947.
KEGGihsa:947.
UCSCiuc001hgw.2. human. [P28906-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

CD34 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81104 mRNA. Translation: AAA03181.1. Different initiation.
M81945
, M81938, M81939, M81940, M81941, M81942, M81943, M81944 Genomic DNA. Translation: AAA03659.1.
S53910 mRNA. Translation: AAB25222.1.
S53911 mRNA. Translation: AAB25223.1.
AF523361 mRNA. Translation: AAM82157.1.
AK291529 mRNA. Translation: BAF84218.1.
AL356275 Genomic DNA. Translation: CAI40195.1.
AL356275 Genomic DNA. Translation: CAI40196.1.
CH471100 Genomic DNA. Translation: EAW93459.1.
BC039146 mRNA. Translation: AAH39146.2.
CCDSiCCDS31011.1. [P28906-1]
CCDS31012.1. [P28906-2]
PIRiA38078.
I67604.
RefSeqiNP_001020280.1. NM_001025109.1. [P28906-1]
NP_001764.1. NM_001773.2. [P28906-2]
UniGeneiHs.374990.

3D structure databases

ProteinModelPortaliP28906.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107385. 1 interactor.
IntActiP28906. 1 interactor.
STRINGi9606.ENSP00000310036.

PTM databases

iPTMnetiP28906.
PhosphoSitePlusiP28906.

Polymorphism and mutation databases

BioMutaiCD34.
DMDMi3183511.

Proteomic databases

EPDiP28906.
MaxQBiP28906.
PaxDbiP28906.
PeptideAtlasiP28906.
PRIDEiP28906.

Protocols and materials databases

DNASUi947.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310833; ENSP00000310036; ENSG00000174059. [P28906-1]
ENST00000356522; ENSP00000348916; ENSG00000174059. [P28906-2]
GeneIDi947.
KEGGihsa:947.
UCSCiuc001hgw.2. human. [P28906-1]

Organism-specific databases

CTDi947.
DisGeNETi947.
GeneCardsiCD34.
HGNCiHGNC:1662. CD34.
HPAiCAB000018.
HPA036722.
HPA036723.
MIMi142230. gene.
neXtProtiNX_P28906.
OpenTargetsiENSG00000174059.
PharmGKBiPA26211.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPJJ. Eukaryota.
ENOG410YPR6. LUCA.
GeneTreeiENSGT00390000008414.
HOVERGENiHBG003461.
InParanoidiP28906.
KOiK06474.
OMAiGMPRGWT.
OrthoDBiEOG091G0K1S.
PhylomeDBiP28906.
TreeFamiTF335795.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000174059-MONOMER.
ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
SIGNORiP28906.

Miscellaneous databases

ChiTaRSiCD34. human.
GeneWikiiCD34.
GenomeRNAii947.
PROiP28906.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174059.
CleanExiHS_CD34.
ExpressionAtlasiP28906. baseline and differential.
GenevisibleiP28906. HS.

Family and domain databases

InterProiIPR008083. CD34.
IPR013836. CD34/Podocalyxin.
[Graphical view]
PANTHERiPTHR16677. PTHR16677. 1 hit.
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]
PRINTSiPR01700. CD34ANTIGEN.
ProtoNetiSearch...

Entry informationi

Entry nameiCD34_HUMAN
AccessioniPrimary (citable) accession number: P28906
Secondary accession number(s): A8K664
, Q15970, Q15971, Q5JTA3, Q5JTA4, Q9UJB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.