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P28905

- HOLC_ECOLI

UniProt

P28905 - HOLC_ECOLI

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Protein
DNA polymerase III subunit chi
Gene
holC, b4259, JW4216
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. positive regulation of DNA-dependent DNA replication initiation Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG11413-MONOMER.
ECOL316407:JW4216-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit chi (EC:2.7.7.7)
Gene namesi
Name:holC
Ordered Locus Names:b4259, JW4216
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11413. holC.

Subcellular locationi

GO - Cellular componenti

  1. DNA polymerase III, clamp loader chi/psi subcomplex Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147DNA polymerase III subunit chi
PRO_0000105517Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP28905.

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaXP0671013EBI-549169,EBI-549140
holBP286315EBI-549169,EBI-549161
holDP2863222EBI-549169,EBI-549176
ssbP0AGE09EBI-549169,EBI-1118620

Protein-protein interaction databases

DIPiDIP-9933N.
IntActiP28905. 41 interactions.
MINTiMINT-1224072.
STRINGi511145.b4259.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86
Helixi20 – 3415
Beta strandi39 – 424
Helixi46 – 5510
Turni56 – 583
Beta strandi67 – 693
Beta strandi79 – 835
Beta strandi94 – 985
Helixi105 – 1095
Beta strandi111 – 1177
Helixi121 – 1233
Helixi124 – 13613
Beta strandi140 – 1445

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EM8X-ray2.10A/C1-147[»]
3SXUX-ray1.85A1-147[»]
ProteinModelPortaliP28905.
SMRiP28905. Positions 1-147.

Miscellaneous databases

EvolutionaryTraceiP28905.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG2927.
HOGENOMiHOG000263567.
KOiK02339.
OMAiWQFEPSA.
OrthoDBiEOG68H887.

Family and domain databases

Gene3Di3.40.50.10110. 1 hit.
InterProiIPR007459. DNA_pol3_chi.
[Graphical view]
PfamiPF04364. DNA_pol3_chi. 1 hit.
[Graphical view]
SUPFAMiSSF102400. SSF102400. 1 hit.

Sequencei

Sequence statusi: Complete.

P28905-1 [UniParc]FASTAAdd to Basket

« Hide

MKNATFYLLD NDTTVDGLSA VEQLVCEIAA ERWRSGKRVL IACEDEKQAY    50
RLDEALWARP AESFVPHNLA GEGPRGGAPV EIAWPQKRSS SRRDILISLR 100
TSFADFATAF TEVVDFVPYE DSLKQLARER YKAYRVAGFN LNTATWK 147
Length:147
Mass (Da):16,633
Last modified:December 1, 1992 - v1
Checksum:i7FEFFC359B28AA88
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04574 Genomic DNA. Translation: AAA70368.1.
Z14155 Genomic DNA. Translation: CAA78524.1.
U14003 Genomic DNA. Translation: AAA97156.1.
U00096 Genomic DNA. Translation: AAC77216.1.
AP009048 Genomic DNA. Translation: BAE78256.1.
X15130 Genomic DNA. Translation: CAA33226.1.
PIRiA46739.
RefSeqiNP_418680.1. NC_000913.3.
YP_492397.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77216; AAC77216; b4259.
BAE78256; BAE78256; BAE78256.
GeneIDi12933731.
948787.
KEGGiecj:Y75_p4142.
eco:b4259.
PATRICi32124089. VBIEscCol129921_4389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04574 Genomic DNA. Translation: AAA70368.1 .
Z14155 Genomic DNA. Translation: CAA78524.1 .
U14003 Genomic DNA. Translation: AAA97156.1 .
U00096 Genomic DNA. Translation: AAC77216.1 .
AP009048 Genomic DNA. Translation: BAE78256.1 .
X15130 Genomic DNA. Translation: CAA33226.1 .
PIRi A46739.
RefSeqi NP_418680.1. NC_000913.3.
YP_492397.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EM8 X-ray 2.10 A/C 1-147 [» ]
3SXU X-ray 1.85 A 1-147 [» ]
ProteinModelPortali P28905.
SMRi P28905. Positions 1-147.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-9933N.
IntActi P28905. 41 interactions.
MINTi MINT-1224072.
STRINGi 511145.b4259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77216 ; AAC77216 ; b4259 .
BAE78256 ; BAE78256 ; BAE78256 .
GeneIDi 12933731.
948787.
KEGGi ecj:Y75_p4142.
eco:b4259.
PATRICi 32124089. VBIEscCol129921_4389.

Organism-specific databases

EchoBASEi EB1385.
EcoGenei EG11413. holC.

Phylogenomic databases

eggNOGi COG2927.
HOGENOMi HOG000263567.
KOi K02339.
OMAi WQFEPSA.
OrthoDBi EOG68H887.

Enzyme and pathway databases

BioCyci EcoCyc:EG11413-MONOMER.
ECOL316407:JW4216-MONOMER.

Miscellaneous databases

EvolutionaryTracei P28905.
PROi P28905.

Gene expression databases

Genevestigatori P28905.

Family and domain databases

Gene3Di 3.40.50.10110. 1 hit.
InterProi IPR007459. DNA_pol3_chi.
[Graphical view ]
Pfami PF04364. DNA_pol3_chi. 1 hit.
[Graphical view ]
SUPFAMi SSF102400. SSF102400. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi."
    Xiao H., Crombie R., Dong Z., Onrust R., O'Donnell M.
    J. Biol. Chem. 268:11773-11778(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli."
    Carter J.R., Franden M.A., Lippincott J., McHenry C.S.
    Mol. Gen. Genet. 241:399-408(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase."
    Stirling C.J., Colloms S., Collins J.F., Szatmari G., Sherratt D.J.
    EMBO J. 8:1623-1627(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-102.
    Strain: K12.
  7. "DNA polymerase III accessory proteins. IV. Characterization of chi and psi."
    Xiao H., Dong Z., O'Donnell M.
    J. Biol. Chem. 268:11779-11784(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Accessory protein function in the DNA polymerase III holoenzyme from E. coli."
    O'Donnell M.
    Bioessays 14:105-111(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiHOLC_ECOLI
AccessioniPrimary (citable) accession number: P28905
Secondary accession number(s): P11649, Q2M650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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