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P28905 (HOLC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit chi

EC=2.7.7.7
Gene names
Name:holC
Ordered Locus Names:b4259, JW4216
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 147147DNA polymerase III subunit chi
PRO_0000105517

Secondary structure

......................... 147
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P28905 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 7FEFFC359B28AA88

FASTA14716,633
        10         20         30         40         50         60 
MKNATFYLLD NDTTVDGLSA VEQLVCEIAA ERWRSGKRVL IACEDEKQAY RLDEALWARP 

        70         80         90        100        110        120 
AESFVPHNLA GEGPRGGAPV EIAWPQKRSS SRRDILISLR TSFADFATAF TEVVDFVPYE 

       130        140 
DSLKQLARER YKAYRVAGFN LNTATWK 

« Hide

References

« Hide 'large scale' references
[1]"DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi."
Xiao H., Crombie R., Dong Z., Onrust R., O'Donnell M.
J. Biol. Chem. 268:11773-11778(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli."
Carter J.R., Franden M.A., Lippincott J., McHenry C.S.
Mol. Gen. Genet. 241:399-408(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
Strain: K12 / MG1655 / ATCC 47076.
[3]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase."
Stirling C.J., Colloms S., Collins J.F., Szatmari G., Sherratt D.J.
EMBO J. 8:1623-1627(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-102.
Strain: K12.
[7]"DNA polymerase III accessory proteins. IV. Characterization of chi and psi."
Xiao H., Dong Z., O'Donnell M.
J. Biol. Chem. 268:11779-11784(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Accessory protein function in the DNA polymerase III holoenzyme from E. coli."
O'Donnell M.
Bioessays 14:105-111(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04574 Genomic DNA. Translation: AAA70368.1.
Z14155 Genomic DNA. Translation: CAA78524.1.
U14003 Genomic DNA. Translation: AAA97156.1.
U00096 Genomic DNA. Translation: AAC77216.1.
AP009048 Genomic DNA. Translation: BAE78256.1.
X15130 Genomic DNA. Translation: CAA33226.1.
PIRA46739.
RefSeqNP_418680.1. NC_000913.3.
YP_492397.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EM8X-ray2.10A/C1-147[»]
3SXUX-ray1.85A1-147[»]
ProteinModelPortalP28905.
SMRP28905. Positions 1-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9933N.
IntActP28905. 41 interactions.
MINTMINT-1224072.
STRING511145.b4259.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77216; AAC77216; b4259.
BAE78256; BAE78256; BAE78256.
GeneID12933731.
948787.
KEGGecj:Y75_p4142.
eco:b4259.
PATRIC32124089. VBIEscCol129921_4389.

Organism-specific databases

EchoBASEEB1385.
EcoGeneEG11413. holC.

Phylogenomic databases

eggNOGCOG2927.
HOGENOMHOG000263567.
KOK02339.
OMAWQFEPSA.
OrthoDBEOG68H887.

Enzyme and pathway databases

BioCycEcoCyc:EG11413-MONOMER.
ECOL316407:JW4216-MONOMER.

Gene expression databases

GenevestigatorP28905.

Family and domain databases

Gene3D3.40.50.10110. 1 hit.
InterProIPR007459. DNA_pol3_chi.
[Graphical view]
PfamPF04364. DNA_pol3_chi. 1 hit.
[Graphical view]
SUPFAMSSF102400. SSF102400. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP28905.
PROP28905.

Entry information

Entry nameHOLC_ECOLI
AccessionPrimary (citable) accession number: P28905
Secondary accession number(s): P11649, Q2M650
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene