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P28905

- HOLC_ECOLI

UniProt

P28905 - HOLC_ECOLI

Protein

DNA polymerase III subunit chi

Gene

holC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. positive regulation of DNA-dependent DNA replication initiation Source: EcoliWiki

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11413-MONOMER.
    ECOL316407:JW4216-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase III subunit chi (EC:2.7.7.7)
    Gene namesi
    Name:holC
    Ordered Locus Names:b4259, JW4216
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11413. holC.

    Subcellular locationi

    GO - Cellular componenti

    1. DNA polymerase III, clamp loader chi/psi subcomplex Source: EcoliWiki

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147DNA polymerase III subunit chiPRO_0000105517Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP28905.

    Interactioni

    Subunit structurei

    The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    dnaXP0671013EBI-549169,EBI-549140
    holBP286315EBI-549169,EBI-549161
    holDP2863222EBI-549169,EBI-549176
    ssbP0AGE09EBI-549169,EBI-1118620

    Protein-protein interaction databases

    DIPiDIP-9933N.
    IntActiP28905. 41 interactions.
    MINTiMINT-1224072.
    STRINGi511145.b4259.

    Structurei

    Secondary structure

    1
    147
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Helixi20 – 3415
    Beta strandi39 – 424
    Helixi46 – 5510
    Turni56 – 583
    Beta strandi67 – 693
    Beta strandi79 – 835
    Beta strandi94 – 985
    Helixi105 – 1095
    Beta strandi111 – 1177
    Helixi121 – 1233
    Helixi124 – 13613
    Beta strandi140 – 1445

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EM8X-ray2.10A/C1-147[»]
    3SXUX-ray1.85A1-147[»]
    ProteinModelPortaliP28905.
    SMRiP28905. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP28905.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG2927.
    HOGENOMiHOG000263567.
    KOiK02339.
    OMAiWQFEPSA.
    OrthoDBiEOG68H887.

    Family and domain databases

    Gene3Di3.40.50.10110. 1 hit.
    InterProiIPR007459. DNA_pol3_chi.
    [Graphical view]
    PfamiPF04364. DNA_pol3_chi. 1 hit.
    [Graphical view]
    SUPFAMiSSF102400. SSF102400. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P28905-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNATFYLLD NDTTVDGLSA VEQLVCEIAA ERWRSGKRVL IACEDEKQAY    50
    RLDEALWARP AESFVPHNLA GEGPRGGAPV EIAWPQKRSS SRRDILISLR 100
    TSFADFATAF TEVVDFVPYE DSLKQLARER YKAYRVAGFN LNTATWK 147
    Length:147
    Mass (Da):16,633
    Last modified:December 1, 1992 - v1
    Checksum:i7FEFFC359B28AA88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04574 Genomic DNA. Translation: AAA70368.1.
    Z14155 Genomic DNA. Translation: CAA78524.1.
    U14003 Genomic DNA. Translation: AAA97156.1.
    U00096 Genomic DNA. Translation: AAC77216.1.
    AP009048 Genomic DNA. Translation: BAE78256.1.
    X15130 Genomic DNA. Translation: CAA33226.1.
    PIRiA46739.
    RefSeqiNP_418680.1. NC_000913.3.
    YP_492397.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77216; AAC77216; b4259.
    BAE78256; BAE78256; BAE78256.
    GeneIDi12933731.
    948787.
    KEGGiecj:Y75_p4142.
    eco:b4259.
    PATRICi32124089. VBIEscCol129921_4389.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04574 Genomic DNA. Translation: AAA70368.1 .
    Z14155 Genomic DNA. Translation: CAA78524.1 .
    U14003 Genomic DNA. Translation: AAA97156.1 .
    U00096 Genomic DNA. Translation: AAC77216.1 .
    AP009048 Genomic DNA. Translation: BAE78256.1 .
    X15130 Genomic DNA. Translation: CAA33226.1 .
    PIRi A46739.
    RefSeqi NP_418680.1. NC_000913.3.
    YP_492397.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EM8 X-ray 2.10 A/C 1-147 [» ]
    3SXU X-ray 1.85 A 1-147 [» ]
    ProteinModelPortali P28905.
    SMRi P28905. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9933N.
    IntActi P28905. 41 interactions.
    MINTi MINT-1224072.
    STRINGi 511145.b4259.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77216 ; AAC77216 ; b4259 .
    BAE78256 ; BAE78256 ; BAE78256 .
    GeneIDi 12933731.
    948787.
    KEGGi ecj:Y75_p4142.
    eco:b4259.
    PATRICi 32124089. VBIEscCol129921_4389.

    Organism-specific databases

    EchoBASEi EB1385.
    EcoGenei EG11413. holC.

    Phylogenomic databases

    eggNOGi COG2927.
    HOGENOMi HOG000263567.
    KOi K02339.
    OMAi WQFEPSA.
    OrthoDBi EOG68H887.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11413-MONOMER.
    ECOL316407:JW4216-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P28905.
    PROi P28905.

    Gene expression databases

    Genevestigatori P28905.

    Family and domain databases

    Gene3Di 3.40.50.10110. 1 hit.
    InterProi IPR007459. DNA_pol3_chi.
    [Graphical view ]
    Pfami PF04364. DNA_pol3_chi. 1 hit.
    [Graphical view ]
    SUPFAMi SSF102400. SSF102400. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi."
      Xiao H., Crombie R., Dong Z., Onrust R., O'Donnell M.
      J. Biol. Chem. 268:11773-11778(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli."
      Carter J.R., Franden M.A., Lippincott J., McHenry C.S.
      Mol. Gen. Genet. 241:399-408(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase."
      Stirling C.J., Colloms S., Collins J.F., Szatmari G., Sherratt D.J.
      EMBO J. 8:1623-1627(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-102.
      Strain: K12.
    7. "DNA polymerase III accessory proteins. IV. Characterization of chi and psi."
      Xiao H., Dong Z., O'Donnell M.
      J. Biol. Chem. 268:11779-11784(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. "Accessory protein function in the DNA polymerase III holoenzyme from E. coli."
      O'Donnell M.
      Bioessays 14:105-111(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiHOLC_ECOLI
    AccessioniPrimary (citable) accession number: P28905
    Secondary accession number(s): P11649, Q2M650
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: June 11, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3