DNA polymerase III subunit chi - Escherichia coli (strain K12)

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P28905 (HOLC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit chi
EC=2.7.7.7

Gene names

Name:	holC
Ordered Locus Names:	b4259, JW4216

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Ontologies

Keywords
Biological process	DNA replication
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
holD	P28632	5	EBI-549169,EBI-549176

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 147

147

DNA polymerase III subunit chi

PRO_0000105517

Secondary structure

147

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P28905 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 7FEFFC359B28AA88
Show »

FASTA

147

16,633

        10         20         30         40         50         60 
MKNATFYLLD NDTTVDGLSA VEQLVCEIAA ERWRSGKRVL IACEDEKQAY RLDEALWARP 

        70         80         90        100        110        120 
AESFVPHNLA GEGPRGGAPV EIAWPQKRSS SRRDILISLR TSFADFATAF TEVVDFVPYE 

       130        140 
DSLKQLARER YKAYRVAGFN LNTATWK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi." Xiao H., Crombie R., Dong Z., Onrust R., O'Donnell M. J. Biol. Chem. 268:11773-11778(1993) [PubMed: 8389364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli." Carter J.R., Franden M.A., Lippincott J., McHenry C.S. Mol. Gen. Genet. 241:399-408(1993) [PubMed: 8246893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase." Stirling C.J., Colloms S., Collins J.F., Szatmari G., Sherratt D.J. EMBO J. 8:1623-1627(1989) [PubMed: 2670557] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-102. Strain: K12.
[7]	"DNA polymerase III accessory proteins. IV. Characterization of chi and psi." Xiao H., Dong Z., O'Donnell M. J. Biol. Chem. 268:11779-11784(1993) [PubMed: 8505305] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Accessory protein function in the DNA polymerase III holoenzyme from E. coli." O'Donnell M. Bioessays 14:105-111(1992) [PubMed: 1575709] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L04574 Genomic DNA. Translation: AAA70368.1.
Z14155 Genomic DNA. Translation: CAA78524.1.
U14003 Genomic DNA. Translation: AAA97156.1.
U00096 Genomic DNA. Translation: AAC77216.1.
AP009048 Genomic DNA. Translation: BAE78256.1.
X15130 Genomic DNA. Translation: CAA33226.1.

PIR

A46739.

RefSeq

NP_418680.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EM8	X-ray	2.10	A/C	1-147	[»]
3SXU	X-ray	1.85	A	1-147	[»]

ProteinModelPortal

P28905.

SMR

P28905. Positions 1-147.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9933N.

IntAct

P28905. 38 interactions.

MINT

MINT-1224072.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002991; EBESCP00000002991; EBESCG00000002450.
EBESCT00000014276; EBESCP00000013567; EBESCG00000013338.

GeneID

948787.

GenomeReviews

Gene locus JW4216 in contig AP009048_GR.
Gene locus b4259 in contig U00096_GR.

KEGG

ecj:JW4216.
eco:b4259.

PATRIC

32124089. VBIEscCol129921_4389.

Organism-specific databases

EchoBASE

EB1385.

EcoGene

EG11413. holC.

Phylogenomic databases

eggNOG

COG2927.

GeneTree

EBGT00050000011700.

HOGENOM

HBG576341.

OMA

IDFVPYE.

ProtClustDB

PRK05728.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11413-MONOMER.

Gene expression databases

Genevestigator

P28905.

Family and domain databases

InterPro

IPR007459. DNA_pol3_chi.
[Graphical view]

Gene3D

G3DSA:3.40.50.10110. DNA_pol3_chi. 1 hit.

K02339.

Pfam

PF04364. DNA_pol3_chi. 1 hit.
[Graphical view]

SUPFAM

SSF102400. DNA_pol3_chi. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HOLC_ECOLI

Accession

Primary (citable) accession number: P28905
Secondary accession number(s): P11649, Q2M650

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents