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Protein

DNA polymerase III subunit chi

Gene

holC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG11413-MONOMER.
ECOL316407:JW4216-MONOMER.
MetaCyc:EG11413-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit chi (EC:2.7.7.7)
Gene namesi
Name:holC
Ordered Locus Names:b4259, JW4216
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11413. holC.

Subcellular locationi

GO - Cellular componenti

  • DNA polymerase III, clamp loader chi/psi subcomplex Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001055171 – 147DNA polymerase III subunit chiAdd BLAST147

Proteomic databases

PaxDbiP28905.
PRIDEiP28905.

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaXP0671013EBI-549169,EBI-549140
holBP286315EBI-549169,EBI-549161
holDP2863222EBI-549169,EBI-549176
ssbP0AGE09EBI-549169,EBI-1118620

Protein-protein interaction databases

BioGridi4262725. 156 interactors.
DIPiDIP-9933N.
IntActiP28905. 41 interactors.
MINTiMINT-1224072.
STRINGi511145.b4259.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi20 – 34Combined sources15
Beta strandi39 – 42Combined sources4
Helixi46 – 55Combined sources10
Turni56 – 58Combined sources3
Beta strandi67 – 69Combined sources3
Beta strandi79 – 83Combined sources5
Beta strandi94 – 98Combined sources5
Helixi105 – 109Combined sources5
Beta strandi111 – 117Combined sources7
Helixi121 – 123Combined sources3
Helixi124 – 136Combined sources13
Beta strandi140 – 144Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EM8X-ray2.10A/C1-147[»]
3SXUX-ray1.85A1-147[»]
ProteinModelPortaliP28905.
SMRiP28905.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28905.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105MYG. Bacteria.
COG2927. LUCA.
HOGENOMiHOG000263567.
InParanoidiP28905.
KOiK02339.
OMAiWQFEPSA.

Family and domain databases

Gene3Di3.40.50.10110. 1 hit.
InterProiIPR007459. DNA_pol3_chi.
[Graphical view]
PfamiPF04364. DNA_pol3_chi. 1 hit.
[Graphical view]
SUPFAMiSSF102400. SSF102400. 1 hit.

Sequencei

Sequence statusi: Complete.

P28905-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNATFYLLD NDTTVDGLSA VEQLVCEIAA ERWRSGKRVL IACEDEKQAY
60 70 80 90 100
RLDEALWARP AESFVPHNLA GEGPRGGAPV EIAWPQKRSS SRRDILISLR
110 120 130 140
TSFADFATAF TEVVDFVPYE DSLKQLARER YKAYRVAGFN LNTATWK
Length:147
Mass (Da):16,633
Last modified:December 1, 1992 - v1
Checksum:i7FEFFC359B28AA88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04574 Genomic DNA. Translation: AAA70368.1.
Z14155 Genomic DNA. Translation: CAA78524.1.
U14003 Genomic DNA. Translation: AAA97156.1.
U00096 Genomic DNA. Translation: AAC77216.1.
AP009048 Genomic DNA. Translation: BAE78256.1.
X15130 Genomic DNA. Translation: CAA33226.1.
PIRiA46739.
RefSeqiNP_418680.1. NC_000913.3.
WP_000786400.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77216; AAC77216; b4259.
BAE78256; BAE78256; BAE78256.
GeneIDi948787.
KEGGiecj:JW4216.
eco:b4259.
PATRICi32124089. VBIEscCol129921_4389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04574 Genomic DNA. Translation: AAA70368.1.
Z14155 Genomic DNA. Translation: CAA78524.1.
U14003 Genomic DNA. Translation: AAA97156.1.
U00096 Genomic DNA. Translation: AAC77216.1.
AP009048 Genomic DNA. Translation: BAE78256.1.
X15130 Genomic DNA. Translation: CAA33226.1.
PIRiA46739.
RefSeqiNP_418680.1. NC_000913.3.
WP_000786400.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EM8X-ray2.10A/C1-147[»]
3SXUX-ray1.85A1-147[»]
ProteinModelPortaliP28905.
SMRiP28905.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262725. 156 interactors.
DIPiDIP-9933N.
IntActiP28905. 41 interactors.
MINTiMINT-1224072.
STRINGi511145.b4259.

Proteomic databases

PaxDbiP28905.
PRIDEiP28905.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77216; AAC77216; b4259.
BAE78256; BAE78256; BAE78256.
GeneIDi948787.
KEGGiecj:JW4216.
eco:b4259.
PATRICi32124089. VBIEscCol129921_4389.

Organism-specific databases

EchoBASEiEB1385.
EcoGeneiEG11413. holC.

Phylogenomic databases

eggNOGiENOG4105MYG. Bacteria.
COG2927. LUCA.
HOGENOMiHOG000263567.
InParanoidiP28905.
KOiK02339.
OMAiWQFEPSA.

Enzyme and pathway databases

BioCyciEcoCyc:EG11413-MONOMER.
ECOL316407:JW4216-MONOMER.
MetaCyc:EG11413-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP28905.
PROiP28905.

Family and domain databases

Gene3Di3.40.50.10110. 1 hit.
InterProiIPR007459. DNA_pol3_chi.
[Graphical view]
PfamiPF04364. DNA_pol3_chi. 1 hit.
[Graphical view]
SUPFAMiSSF102400. SSF102400. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHOLC_ECOLI
AccessioniPrimary (citable) accession number: P28905
Secondary accession number(s): P11649, Q2M650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.