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Protein

Trehalose-6-phosphate hydrolase

Gene

treC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes trehalose-6-phosphate to glucose and glucose 6-phosphate. Can also very effectively hydrolyzes p-nitrophenyl-alpha-D-glucopyranoside, but it does not recognize trehalose, sucrose, maltose, isomaltose, or maltodextrins.1 Publication

Catalytic activityi

Alpha,alpha-trehalose 6-phosphate + H2O = D-glucose + D-glucose 6-phosphate.1 Publication

Kineticsi

  1. KM=6 mM for trehalose-6-phosphate1 Publication
  1. Vmax=5.5 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei200NucleophileBy similarity1
Active sitei251Proton donorBy similarity1
Sitei325Transition state stabilizerBy similarity1

GO - Molecular functioni

  • alpha,alpha-phosphotrehalase activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • trehalose catabolic process Source: EcoCyc

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:TRE6PHYDRO-MONOMER
MetaCyc:TRE6PHYDRO-MONOMER

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate hydrolase1 Publication (EC:3.2.1.931 Publication)
Alternative name(s):
Alpha,alpha-phosphotrehalase
Gene namesi
Name:treC
Synonyms:olgH
Ordered Locus Names:b4239, JW4198
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11402 treC

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543191 – 551Trehalose-6-phosphate hydrolaseAdd BLAST551

Proteomic databases

PaxDbiP28904
PRIDEiP28904

Interactioni

Protein-protein interaction databases

BioGridi4259319, 17 interactors
DIPiDIP-11024N
IntActiP28904, 8 interactors
STRINGi316385.ECDH10B_4434

Structurei

3D structure databases

ProteinModelPortaliP28904
SMRiP28904
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4105CG3 Bacteria
COG0366 LUCA
HOGENOMiHOG000220641
InParanoidiP28904
KOiK01226
OMAiVDVQKTQ
PhylomeDBiP28904

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR022567 DUF3459
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR012769 Trehalose_TreC
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF11941 DUF3459, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
TIGRFAMsiTIGR02403 trehalose_treC, 1 hit

Sequencei

Sequence statusi: Complete.

P28904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHLPHWWQN GVIYQIYPKS FQDTTGSGTG DLRGVIQHLD YLHKLGVDAI
60 70 80 90 100
WLTPFYVSPQ VDNGYDVANY TAIDPTYGTL DDFDELVTQA KSRGIRIILD
110 120 130 140 150
MVFNHTSTQH AWFREALNKE SPYRQFYIWR DGEPETPPNN WRSKFGGSAW
160 170 180 190 200
RWHAESEQYY LHLFAPEQAD LNWENPAVRA ELKKVCEFWA DRGVDGLRLD
210 220 230 240 250
VVNLISKDPR FPEDLDGDGR RFYTDGPRAH EFLHEMNRDV FTPRGLMTVG
260 270 280 290 300
EMSSTSLEHC QRYAALTGSE LSMTFNFHHL KVDYPGGEKW TLAKPDFVAL
310 320 330 340 350
KTLFRHWQQG MHNVAWNALF WCNHDQPRIV SRFGDEGEYR VPAAKMLAMV
360 370 380 390 400
LHGMQGTPYI YQGEEIGMTN PHFTRITDYR DVESLNMFAE LRNDGRDADE
410 420 430 440 450
LLAILASKSR DNSRTPMQWS NGDNAGFTAG EPWIGLGDNY QQINVEAALA
460 470 480 490 500
DDSSVFYTYQ KLIALRKQEA ILTWGNYQDL LPNSPVLWCY RREWKGQTLL
510 520 530 540 550
VIANLSREIQ PWQAGQMRGN WQLVMHNYEE ASPQPCAMNL RPFEAVWWLQ

K
Length:551
Mass (Da):63,838
Last modified:February 1, 1995 - v3
Checksum:i975F89F8D91621A6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74D → V in AAC43382 (PubMed:8083158).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06195 Genomic DNA Translation: AAC43382.1
U14003 Genomic DNA Translation: AAA97136.1
U00096 Genomic DNA Translation: AAC77196.1
AP009048 Genomic DNA Translation: BAE78238.1
L06097 Genomic DNA Translation: AAA24225.1
PIRiS56465
RefSeqiNP_418660.1, NC_000913.3
WP_001336303.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77196; AAC77196; b4239
BAE78238; BAE78238; BAE78238
GeneIDi948762
KEGGiecj:JW4198
eco:b4239
PATRICifig|1411691.4.peg.2462

Similar proteinsi

Entry informationi

Entry nameiTREC_ECOLI
AccessioniPrimary (citable) accession number: P28904
Secondary accession number(s): Q2M668
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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