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Protein

Trehalose-6-phosphate hydrolase

Gene

treC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes trehalose-6-phosphate to glucose and glucose 6-phosphate. Can also very effectively hydrolyzes p-nitrophenyl-alpha-D-glucopyranoside, but it does not recognize trehalose, sucrose, maltose, isomaltose, or maltodextrins.1 Publication

Catalytic activityi

Alpha,alpha-trehalose 6-phosphate + H2O = D-glucose + D-glucose 6-phosphate.1 Publication

Kineticsi

  1. KM=6 mM for trehalose-6-phosphate1 Publication
  1. Vmax=5.5 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei200 – 2001NucleophileBy similarity
Active sitei251 – 2511Proton donorBy similarity
Sitei325 – 3251Transition state stabilizerBy similarity

GO - Molecular functioni

  • alpha,alpha-phosphotrehalase activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • trehalose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:TRE6PHYDRO-MONOMER.
ECOL316407:JW4198-MONOMER.
MetaCyc:TRE6PHYDRO-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate hydrolase1 Publication (EC:3.2.1.931 Publication)
Alternative name(s):
Alpha,alpha-phosphotrehalase
Gene namesi
Name:treC
Synonyms:olgH
Ordered Locus Names:b4239, JW4198
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11402. treC.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551Trehalose-6-phosphate hydrolasePRO_0000054319Add
BLAST

Proteomic databases

PaxDbiP28904.
PRIDEiP28904.

Interactioni

Protein-protein interaction databases

BioGridi4259319. 11 interactions.
DIPiDIP-11024N.
IntActiP28904. 8 interactions.
STRINGi511145.b4239.

Structurei

3D structure databases

ProteinModelPortaliP28904.
SMRiP28904. Positions 1-546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4105CG3. Bacteria.
COG0366. LUCA.
HOGENOMiHOG000220641.
InParanoidiP28904.
KOiK01226.
OMAiEHEWFRQ.
OrthoDBiEOG6RVFV2.
PhylomeDBiP28904.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012769. Trehalose_TreC.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR02403. trehalose_treC. 1 hit.

Sequencei

Sequence statusi: Complete.

P28904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHLPHWWQN GVIYQIYPKS FQDTTGSGTG DLRGVIQHLD YLHKLGVDAI
60 70 80 90 100
WLTPFYVSPQ VDNGYDVANY TAIDPTYGTL DDFDELVTQA KSRGIRIILD
110 120 130 140 150
MVFNHTSTQH AWFREALNKE SPYRQFYIWR DGEPETPPNN WRSKFGGSAW
160 170 180 190 200
RWHAESEQYY LHLFAPEQAD LNWENPAVRA ELKKVCEFWA DRGVDGLRLD
210 220 230 240 250
VVNLISKDPR FPEDLDGDGR RFYTDGPRAH EFLHEMNRDV FTPRGLMTVG
260 270 280 290 300
EMSSTSLEHC QRYAALTGSE LSMTFNFHHL KVDYPGGEKW TLAKPDFVAL
310 320 330 340 350
KTLFRHWQQG MHNVAWNALF WCNHDQPRIV SRFGDEGEYR VPAAKMLAMV
360 370 380 390 400
LHGMQGTPYI YQGEEIGMTN PHFTRITDYR DVESLNMFAE LRNDGRDADE
410 420 430 440 450
LLAILASKSR DNSRTPMQWS NGDNAGFTAG EPWIGLGDNY QQINVEAALA
460 470 480 490 500
DDSSVFYTYQ KLIALRKQEA ILTWGNYQDL LPNSPVLWCY RREWKGQTLL
510 520 530 540 550
VIANLSREIQ PWQAGQMRGN WQLVMHNYEE ASPQPCAMNL RPFEAVWWLQ

K
Length:551
Mass (Da):63,838
Last modified:February 1, 1995 - v3
Checksum:i975F89F8D91621A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741D → V in AAC43382 (PubMed:8083158).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06195 Genomic DNA. Translation: AAC43382.1.
U14003 Genomic DNA. Translation: AAA97136.1.
U00096 Genomic DNA. Translation: AAC77196.1.
AP009048 Genomic DNA. Translation: BAE78238.1.
L06097 Genomic DNA. Translation: AAA24225.1.
PIRiS56465.
RefSeqiNP_418660.1. NC_000913.3.
WP_001336303.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77196; AAC77196; b4239.
BAE78238; BAE78238; BAE78238.
GeneIDi948762.
KEGGiecj:JW4198.
eco:b4239.
PATRICi32124053. VBIEscCol129921_4371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06195 Genomic DNA. Translation: AAC43382.1.
U14003 Genomic DNA. Translation: AAA97136.1.
U00096 Genomic DNA. Translation: AAC77196.1.
AP009048 Genomic DNA. Translation: BAE78238.1.
L06097 Genomic DNA. Translation: AAA24225.1.
PIRiS56465.
RefSeqiNP_418660.1. NC_000913.3.
WP_001336303.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP28904.
SMRiP28904. Positions 1-546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259319. 11 interactions.
DIPiDIP-11024N.
IntActiP28904. 8 interactions.
STRINGi511145.b4239.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiP28904.
PRIDEiP28904.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77196; AAC77196; b4239.
BAE78238; BAE78238; BAE78238.
GeneIDi948762.
KEGGiecj:JW4198.
eco:b4239.
PATRICi32124053. VBIEscCol129921_4371.

Organism-specific databases

EchoBASEiEB1374.
EcoGeneiEG11402. treC.

Phylogenomic databases

eggNOGiENOG4105CG3. Bacteria.
COG0366. LUCA.
HOGENOMiHOG000220641.
InParanoidiP28904.
KOiK01226.
OMAiEHEWFRQ.
OrthoDBiEOG6RVFV2.
PhylomeDBiP28904.

Enzyme and pathway databases

BioCyciEcoCyc:TRE6PHYDRO-MONOMER.
ECOL316407:JW4198-MONOMER.
MetaCyc:TRE6PHYDRO-MONOMER.

Miscellaneous databases

PROiP28904.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012769. Trehalose_TreC.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR02403. trehalose_treC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Trehalose-6-phosphate hydrolase of Escherichia coli."
    Rimmele M., Boos W.
    J. Bacteriol. 176:5654-5664(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: K12.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A possible glycine radical in anaerobic ribonucleotide reductase from Escherichia coli: nucleotide sequence of the cloned nrdD gene."
    Sun X., Harder J., Krook M., Joernvall H., Sjoeberg B.-M., Reichard P.
    Proc. Natl. Acad. Sci. U.S.A. 90:577-581(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 160-392.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiTREC_ECOLI
AccessioniPrimary (citable) accession number: P28904
Secondary accession number(s): Q2M668
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.