Reviewed,
UniProtKB/Swiss-Prot P28903 (NRDD_ECOLI)
Last modified
June 16, 2009.
Version 78.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Anaerobic ribonucleoside-triphosphate reductase EC=1.17.4.2 | ||||
| Gene names |
| ||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 712 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | 2'-deoxyribonucleoside triphosphate + thioredoxin disulfide + H2O = ribonucleoside triphosphate + thioredoxin. Ref.7 |
| Subunit structure | Tetramer consisting of 2 alpha (NrdD) and 2 beta (NrdG) subunits. |
| Induction | Probably by nrdD-activating enzyme under anaerobic conditions by generation of an organic free radical. Exposure of activated nrdD to oxygen may result in cleavage at the glycine residue harboring its organic radical with loss of the 31 C-terminal AA. |
| Sequence similarities | Belongs to the anaerobic ribonucleoside-triphosphate reductase family. Contains 1 ATP-cone domain. Contains 1 glycine radical domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | ATP-binding Metal-binding Nucleotide-binding Zinc |
| Molecular function | Oxidoreductase |
| PTM | Organic radical |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct ribonucleoside-triphosphate reductase activityInferred from electronic annotation. Source: EC zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 712 | 712 | Anaerobic ribonucleoside-triphosphate reductase | PRO_0000166683 | |||||
Regions | |||||||||
| Domain | 3 – 92 | 90 | ATP-cone | ||||||
| Domain | 583 – 708 | 126 | Glycine radical | ||||||
Sites | |||||||||
| Metal binding | 644 | 1 | Zinc | ||||||
| Metal binding | 647 | 1 | Zinc | ||||||
| Metal binding | 662 | 1 | Zinc | ||||||
| Metal binding | 665 | 1 | Zinc | ||||||
Amino acid modifications | |||||||||
| Modified residue | 681 | 1 | Glycine radical By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 257 | 1 | G → R in AAA24226. Ref.1 | ||||||
| Sequence conflict | 420 | 1 | A → P in AAA24226. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A possible glycine radical in anaerobic ribonucleotide reductase from Escherichia coli: nucleotide sequence of the cloned nrdD gene." Sun X., Harder J., Krook M., Joernvall H., Sjoeberg B.-M., Reichard P. Proc. Natl. Acad. Sci. U.S.A. 90:577-581(1993) [PubMed: 8421692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [2] | "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Trehalose-6-phosphate hydrolase of Escherichia coli." Rimmele M., Boos W. J. Bacteriol. 176:5654-5664(1994) [PubMed: 8083158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-229. Strain: K12. |
| [6] | "Generation of the glycyl radical of the anaerobic Escherichia coli ribonucleotide reductase requires a specific activating enzyme." Sun X., Eliasson R., Pontis E., Andersson J., Buist G., Sjoeberg B.-M., Reichard P. J. Biol. Chem. 270:2443-2446(1995) [PubMed: 7852304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 703-712. Strain: K12. |
| [7] | "A metal-binding site in the catalytic subunit of anaerobic ribonucleotide reductase." Logan D.T., Mulliez E., Larsson K.-M., Bodevin S., Atta M., Garnaud P.E., Sjoeberg B.-M., Fontecave M. Proc. Natl. Acad. Sci. U.S.A. 100:3826-3831(2003) [PubMed: 12655046] [Abstract] Cited for: MUTAGENESIS OF CYSTEINE RESIDUES, ENZYME ACTIVITY. |
Cross-references
Sequence databases | |
|---|---|
| L06097 Genomic DNA. Translation: AAA24226.1. U14003 Genomic DNA. Translation: AAA97135.1. U00096 Genomic DNA. Translation: AAC77195.1. AP009048 Genomic DNA. Translation: BAE78237.1. U06195 Genomic DNA. Translation: AAC43383.1. Z46865 Genomic DNA. Translation: CAA86938.1. | |
| PIR | A47331. |
| RefSeq | AP_004736.1. NP_418659.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1H78 based on UniProtKB P07071. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P28903. 3 interactions. |
Genome annotation databases | |
| GeneID | 948755. |
| GenomeReviews | Gene locus JW4197 in contig AP009048_GR. Gene locus b4238 in contig U00096_GR. |
| KEGG | ecj:JW4197. eco:b4238. |
Organism-specific databases | |
| EchoBASE | EB1388. |
| EcoGene | EG11417. nrdD. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P28903. |
| OMA | P28903. PSENLCS. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:RIBONUCLEOSIDE-TRIP-REDUCT-MON. MetaCyc:RIBONUCLEOSIDE-TRIP-REDUCT-MON. |
Family and domain databases | |
| InterPro | IPR005144. ATP-cone. IPR001150. Form_AcTrfase_GR. IPR019777. Form_AcTrfase_GR_CS. IPR012833. NrdD. [Graphical view] |
| Pfam | PF03477. ATP-cone. 1 hit. PF01228. Gly_radical. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR02487. NrdD. 1 hit. |
| PROSITE | PS51161. ATP_CONE. 1 hit. PS00850. GLY_RADICAL_1. 1 hit. PS51149. GLY_RADICAL_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NRDD_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P28903 Secondary accession number(s): Q2M669 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with


