Reviewed, UniProtKB/Swiss-Prot P28901 (FENR_SHIFL)

Names and origin

Protein names

Recommended name:
    Ferredoxin--NADP reductase
      Short name=FNR
    EC=1.18.1.2
Alternative name(s):
    Flavodoxin reductase
      Short name=FLXR
    Methyl viologen resistance protein A

Gene names

Name:	fpr
Synonyms:	mvrA
Ordered Locus Names:	SF4002, S3745

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Together with flavodoxin is involved in the reductive activation of cobalamin-independent methionine synthase, pyruvate formate lyase and anaerobic ribonucleotide reductase. Also protects against superoxide radicals due to methyl viologen in the presence of oxygen By similarity.
Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	FAD.
Sequence similarities	Belongs to the ferredoxin--NADP reductase type 1 family. Contains 1 FAD-binding FR-type domain.
Sequence caution	The sequence CAA77813.1 differs from that shown. Reason: Frameshift at positions 94 and 126.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Ferredoxin--NADP reductase		PRO_0000167644
Regions
	Domain	2 – 101	100	FAD-binding FR-type
	Nucleotide binding	50 – 53	4	FAD By similarity
	Nucleotide binding	73 – 76	4	FAD By similarity
	Nucleotide binding	143 – 144	2	NADP By similarity
	Nucleotide binding	173 – 174	2	NADP By similarity
	Nucleotide binding	213 – 214	2	NADP By similarity
Sites
	Binding site	53	1	NADP By similarity
	Binding site	116	1	FAD By similarity
	Binding site	116	1	NADP; via amide nitrogen By similarity
	Binding site	245	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28901-1 [UniParc]. Last modified January 10, 2003. Version 3. Checksum: 1D71D26F195EA512	FASTA	248	27,699
	10 20 30 40 50 60 MADWVTGKVT KVQNWTDALF SLTVHAPVLP FTAGQFTKLG LEIDGERVQR AYSYVNSPDN 70 80 90 100 110 120 PDLEFYLVTV PDGKLSPRLA ALKPGDEVQV VSEAAGFFVL DEVPDCETLW MLATGTAIGP 130 140 150 160 170 180 YLSILQLGKD LDRFKNLVLV HAARYAADLS YLPLMQELEK RYEGKLRIQT VVSRETAAGS 190 200 210 220 230 240 LTGRIPALIE SGELESAIGL PMNKETSHVM LCGNPQMVRD TQQLLKETRQ MTKHLRRRPG HMTAEHYW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[2]	"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.
[3]	"Molecular analysis of the glpFKX regions of Escherichia coli and Shigella flexneri." Truniger V., Boos W., Sweet G. J. Bacteriol. 174:6981-6991(1992) [PubMed: 1400248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135. Strain: M4243.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN45435.1. AE014073 Genomic DNA. Translation: AAP18765.1. Z11766 Genomic DNA. Translation: CAA77813.1. Frameshift.
PIR	S23906.
RefSeq	NP_709728.1. NP_838954.1.
3D structure databases
HSSP	HSSP built from PDB template 1FDR based on UniProtKB P28861.
SMR	P28901. Positions 2-248.
ModBase	Search...
Genome annotation databases
GeneID	1023471. 1079956.
GenomeReviews	Gene locus SF4002 in contig AE005674_GR. Gene locus S3745 in contig AE014073_GR.
KEGG	sfl:SF4002. sfx:S3745.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P28901.
OMA	P28901. IMLCGNP.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN45435.1-MON.
BRENDA	1.18.1.2. 189495.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FENR_SHIFL

Accession

Primary (citable) accession number: P28901

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 10, 2003
Last modified:	June 16, 2009
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)