Reviewed, UniProtKB/Swiss-Prot P28894 (FUMC1_BRAJA)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II 1
      Short name=Fumarase C 1
    EC=4.2.1.2

Gene names

Name:	fumC1
Synonyms:	fumC
Ordered Locus Names:	blr6519

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Fumarate hydratase class II 1 HAMAP MF_00743		PRO_0000161259
Regions
	Region	139 – 142	4	B site By similarity
	Region	149 – 151	3	Substrate binding By similarity
Sites
	Binding site	110	1	Substrate By similarity
Experimental info
	Sequence conflict	91 – 94	4	IDGS → STAD in AAA26211. Ref.1
	Sequence conflict	91 – 94	4	IDGS → STAD in AAA26212. Ref.1
	Sequence conflict	220	1	S → R in AAA26211. Ref.1
	Sequence conflict	220	1	S → R in AAA26212. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P28894-1 [UniParc]. Last modified December 15, 2003. Version 3. Checksum: CDB35CFA32062957	FASTA	473	50,924
	10 20 30 40 50 60 MAKSARTKTA RPATRTETDS FGPIEVPSDR YWGAQTERSR QNFRIGTDRM PISLVHALGI 70 80 90 100 110 120 VKLAAAQSNR ELGLLDQRRA SAIIRAAREV IDGSLDDHFP LVVWQTGSGT QTNMNLNEVI 130 140 150 160 170 180 ANRANELLGG ELGAKKPVHP NDHVNMSQSS NDSFPTAMHI AAASRITADL VPALGELLRA 190 200 210 220 230 240 LRKKEKEFAK IVKIGRTHTQ DATPLTLGQE FSGYAAQVES GIARLKVAVK ELYPLAQGGT 250 260 270 280 290 300 AVGTGLNAKP RFARLFAKHV AGITKLPFTS AANKFEALAS NDAYVLAHGA ISSVATGLFK 310 320 330 340 350 360 IANDIRLLGS GPRSGLGELI LPENEPGSSI MPGKVNPTQC EAMTMVCCQV FGNHTAITVA 370 380 390 400 410 420 GSQGHFELNV YKPVLAYNML HSIRLMADAA RSFTEHCVSG IRADEKRISE LMQRSLMLVT 430 440 450 460 470 ALAPKIGYDN AAKVAKTAHA NGTTLKEEAL RLGFVTADEF DRLVQPEKMT KPG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and mutational analysis of the Bradyrhizobium japonicum fumC-like gene: evidence for the existence of two different fumarases." Acuna G., Ebeling S., Hennecke H. J. Gen. Microbiol. 137:991-1000(1991) [PubMed: 1856685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M79457 Genomic DNA. Translation: AAA26211.1. Different initiation. M79457 Genomic DNA. Translation: AAA26212.1. BA000040 Genomic DNA. Translation: BAC51784.1. Different initiation.
RefSeq	NP_773159.2.
3D structure databases
SMR	P28894. Positions 15-472.
ModBase	Search...
Genome annotation databases
GeneID	1054478.
GenomeReviews	Gene locus blr6519 in contig BA000040_GR.
KEGG	bja:blr6519.
NMPDR	fig|224911.1.peg.6519.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284369.
OMA	RIEKDTM.
PhylomeDB	P28894.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR6519-MONOMER.
BRENDA	4.2.1.2. 280.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC1_BRAJA

Accession

Primary (citable) accession number: P28894

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)