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Protein

Fumarate hydratase class II 2

Gene

fumC2

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II 1 (fumC1), Fumarate hydratase class II 2 (fumC2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei197SubstrateUniRule annotation1
Active sitei198Proton donor/acceptorUniRule annotation1
Active sitei328UniRule annotation1
Binding sitei329SubstrateUniRule annotation1
Sitei341Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class II 2UniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase C 2UniRule annotation
Alternative name(s):
Aerobic fumarase 2UniRule annotation
Iron-independent fumarase 2UniRule annotation
Gene namesi
Name:fumC2UniRule annotation
Synonyms:fumC
Ordered Locus Names:blr6519
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001612591 – 473Fumarate hydratase class II 2Add BLAST473

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi224911.blr6519.

Structurei

3D structure databases

ProteinModelPortaliP28894.
SMRiP28894.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 110Substrate bindingUniRule annotation3
Regioni139 – 142Substrate binding (B site)UniRule annotation4
Regioni149 – 151Substrate bindingUniRule annotation3
Regioni334 – 336Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
InParanoidiP28894.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

P28894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSARTKTA RPATRTETDS FGPIEVPSDR YWGAQTERSR QNFRIGTDRM
60 70 80 90 100
PISLVHALGI VKLAAAQSNR ELGLLDQRRA SAIIRAAREV IDGSLDDHFP
110 120 130 140 150
LVVWQTGSGT QTNMNLNEVI ANRANELLGG ELGAKKPVHP NDHVNMSQSS
160 170 180 190 200
NDSFPTAMHI AAASRITADL VPALGELLRA LRKKEKEFAK IVKIGRTHTQ
210 220 230 240 250
DATPLTLGQE FSGYAAQVES GIARLKVAVK ELYPLAQGGT AVGTGLNAKP
260 270 280 290 300
RFARLFAKHV AGITKLPFTS AANKFEALAS NDAYVLAHGA ISSVATGLFK
310 320 330 340 350
IANDIRLLGS GPRSGLGELI LPENEPGSSI MPGKVNPTQC EAMTMVCCQV
360 370 380 390 400
FGNHTAITVA GSQGHFELNV YKPVLAYNML HSIRLMADAA RSFTEHCVSG
410 420 430 440 450
IRADEKRISE LMQRSLMLVT ALAPKIGYDN AAKVAKTAHA NGTTLKEEAL
460 470
RLGFVTADEF DRLVQPEKMT KPG
Length:473
Mass (Da):50,924
Last modified:December 15, 2003 - v3
Checksum:iCDB35CFA32062957
GO

Sequence cautioni

The sequence AAA26211 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC51784 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91 – 94IDGS → STAD in AAA26211 (PubMed:1856685).Curated4
Sequence conflicti91 – 94IDGS → STAD in AAA26212 (PubMed:1856685).Curated4
Sequence conflicti220S → R in AAA26211 (PubMed:1856685).Curated1
Sequence conflicti220S → R in AAA26212 (PubMed:1856685).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M79457 Genomic DNA. Translation: AAA26211.1. Different initiation.
M79457 Genomic DNA. Translation: AAA26212.1.
BA000040 Genomic DNA. Translation: BAC51784.1. Different initiation.
RefSeqiNP_773159.2. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC51784; BAC51784; BAC51784.
GeneIDi1054478.
KEGGibja:blr6519.
PATRICifig|224911.5.peg.6668.

Similar proteinsi

Entry informationi

Entry nameiFUMC2_BRADU
AccessioniPrimary (citable) accession number: P28894
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 15, 2003
Last modified: August 30, 2017
This is version 118 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families