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P28894

- FUMC1_BRADU

UniProt

P28894 - FUMC1_BRADU

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Protein

Fumarate hydratase class II 1

Gene
fumC1, fumC, blr6519
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible addition of water to fumarate to give L-malate By similarity.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981Proton donor/acceptor By similarity
Active sitei328 – 3281 By similarity
Binding sitei329 – 3291Substrate By similarity
Sitei341 – 3411Important for catalytic activity By similarity

GO - Molecular functioni

  1. fumarate hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fumarate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-6569-MONOMER.
UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class II 1 (EC:4.2.1.2)
Short name:
Fumarase C 1
Gene namesi
Name:fumC1
Synonyms:fumC
Ordered Locus Names:blr6519
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. tricarboxylic acid cycle enzyme complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Fumarate hydratase class II 1UniRule annotationPRO_0000161259Add
BLAST

Proteomic databases

PRIDEiP28894.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224911.blr6519.

Structurei

3D structure databases

ProteinModelPortaliP28894.
SMRiP28894. Positions 15-472.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni108 – 1103Substrate binding By similarity
Regioni139 – 1424B site By similarity
Regioni149 – 1513Substrate binding By similarity
Regioni197 – 1982Substrate binding By similarity
Regioni334 – 3363Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0114.
HOGENOMiHOG000061736.
KOiK01679.
OrthoDBiEOG6V1M4M.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28894-1 [UniParc]FASTAAdd to Basket

« Hide

MAKSARTKTA RPATRTETDS FGPIEVPSDR YWGAQTERSR QNFRIGTDRM    50
PISLVHALGI VKLAAAQSNR ELGLLDQRRA SAIIRAAREV IDGSLDDHFP 100
LVVWQTGSGT QTNMNLNEVI ANRANELLGG ELGAKKPVHP NDHVNMSQSS 150
NDSFPTAMHI AAASRITADL VPALGELLRA LRKKEKEFAK IVKIGRTHTQ 200
DATPLTLGQE FSGYAAQVES GIARLKVAVK ELYPLAQGGT AVGTGLNAKP 250
RFARLFAKHV AGITKLPFTS AANKFEALAS NDAYVLAHGA ISSVATGLFK 300
IANDIRLLGS GPRSGLGELI LPENEPGSSI MPGKVNPTQC EAMTMVCCQV 350
FGNHTAITVA GSQGHFELNV YKPVLAYNML HSIRLMADAA RSFTEHCVSG 400
IRADEKRISE LMQRSLMLVT ALAPKIGYDN AAKVAKTAHA NGTTLKEEAL 450
RLGFVTADEF DRLVQPEKMT KPG 473
Length:473
Mass (Da):50,924
Last modified:December 15, 2003 - v3
Checksum:iCDB35CFA32062957
GO

Sequence cautioni

The sequence AAA26211.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC51784.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 944IDGS → STAD in AAA26211. 1 Publication
Sequence conflicti91 – 944IDGS → STAD in AAA26212. 1 Publication
Sequence conflicti220 – 2201S → R in AAA26211. 1 Publication
Sequence conflicti220 – 2201S → R in AAA26212. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M79457 Genomic DNA. Translation: AAA26211.1. Different initiation.
M79457 Genomic DNA. Translation: AAA26212.1.
BA000040 Genomic DNA. Translation: BAC51784.1. Different initiation.
RefSeqiNP_773159.2. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC51784; BAC51784; BAC51784.
GeneIDi1054478.
KEGGibja:blr6519.
PATRICi21196868. VBIBraJap65052_6668.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M79457 Genomic DNA. Translation: AAA26211.1 . Different initiation.
M79457 Genomic DNA. Translation: AAA26212.1 .
BA000040 Genomic DNA. Translation: BAC51784.1 . Different initiation.
RefSeqi NP_773159.2. NC_004463.1.

3D structure databases

ProteinModelPortali P28894.
SMRi P28894. Positions 15-472.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr6519.

Proteomic databases

PRIDEi P28894.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC51784 ; BAC51784 ; BAC51784 .
GeneIDi 1054478.
KEGGi bja:blr6519.
PATRICi 21196868. VBIBraJap65052_6668.

Phylogenomic databases

eggNOGi COG0114.
HOGENOMi HOG000061736.
KOi K01679.
OrthoDBi EOG6V1M4M.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01007 .
BioCyci BJAP224911:GJEJ-6569-MONOMER.

Family and domain databases

Gene3Di 1.10.275.10. 1 hit.
HAMAPi MF_00743. FumaraseC.
InterProi IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view ]
PANTHERi PTHR11444. PTHR11444. 1 hit.
Pfami PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view ]
PRINTSi PR00149. FUMRATELYASE.
SUPFAMi SSF48557. SSF48557. 1 hit.
TIGRFAMsi TIGR00979. fumC_II. 1 hit.
PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and mutational analysis of the Bradyrhizobium japonicum fumC-like gene: evidence for the existence of two different fumarases."
    Acuna G., Ebeling S., Hennecke H.
    J. Gen. Microbiol. 137:991-1000(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: USDA 110spc4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiFUMC1_BRADU
AccessioniPrimary (citable) accession number: P28894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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