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Protein

Ferredoxin--NADP reductase

Gene

fpr

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports electrons between flavodoxin or ferredoxin and NADPH. Involved in the reductive activation of cobalamin-independent methionine synthase, pyruvate formate lyase and anaerobic ribonucleotide reductase. Also protects against superoxide radicals due to methyl viologen in the presence of oxygen.

Catalytic activityi

2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53NADPBy similarity1
Binding sitei66FAD; via carbonyl oxygen1 Publication1
Binding sitei116FAD1 Publication1
Binding sitei116NADP; via amide nitrogenBy similarity1
Binding sitei245NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi50 – 53FAD1 Publication4
Nucleotide bindingi74 – 76FAD1 Publication3
Nucleotide bindingi143 – 144NADPBy similarity2
Nucleotide bindingi173 – 174NADPBy similarity2
Nucleotide bindingi213 – 214NADPBy similarity2
Nucleotide bindingi247 – 248FAD1 Publication2

GO - Molecular functioni

  • FAD binding Source: EcoCyc
  • ferredoxin-NADP+ reductase activity Source: EcoCyc
  • oxidoreductase activity Source: EcoliWiki

GO - Biological processi

  • fructose catabolic process Source: EcoliWiki
  • response to drug Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciEcoCyc:FLAVONADPREDUCT-MONOMER.
ECOL316407:JW3895-MONOMER.
MetaCyc:FLAVONADPREDUCT-MONOMER.
BRENDAi1.18.1.2. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin--NADP reductase (EC:1.18.1.2)
Short name:
FNR
Alternative name(s):
DA1
Flavodoxin reductase
Short name:
FLDR
Short name:
FLXR
Methyl viologen resistance protein A
Gene namesi
Name:fpr
Synonyms:mvrA
Ordered Locus Names:b3924, JW3895
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11518. fpr.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

DrugBankiDB03147. Flavin adenine dinucleotide.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001676432 – 248Ferredoxin--NADP reductaseAdd BLAST247

Proteomic databases

EPDiP28861.
PaxDbiP28861.
PRIDEiP28861.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261115. 2 interactors.
IntActiP28861. 6 interactors.
STRINGi511145.b3924.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 14Combined sources11
Beta strandi16 – 25Combined sources10
Beta strandi36 – 41Combined sources6
Beta strandi48 – 53Combined sources6
Beta strandi61 – 68Combined sources8
Helixi76 – 80Combined sources5
Beta strandi87 – 93Combined sources7
Helixi100 – 102Combined sources3
Beta strandi107 – 114Combined sources8
Helixi115 – 118Combined sources4
Helixi119 – 127Combined sources9
Beta strandi135 – 145Combined sources11
Helixi146 – 148Combined sources3
Helixi152 – 161Combined sources10
Turni162 – 164Combined sources3
Beta strandi165 – 175Combined sources11
Beta strandi180 – 183Combined sources4
Helixi185 – 190Combined sources6
Helixi193 – 198Combined sources6
Turni204 – 206Combined sources3
Beta strandi207 – 213Combined sources7
Helixi215 – 229Combined sources15
Beta strandi236 – 238Combined sources3
Beta strandi241 – 246Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FDRX-ray1.70A1-248[»]
2XNJX-ray1.90A/B2-247[»]
ProteinModelPortaliP28861.
SMRiP28861.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28861.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 101FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST100

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DP0. Bacteria.
COG1018. LUCA.
HOGENOMiHOG000265758.
InParanoidiP28861.
KOiK00528.
OMAiPFIAGQF.
PhylomeDBiP28861.

Family and domain databases

CDDicd06195. FNR1. 1 hit.
InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR033892. FNR_bac.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28861-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADWVTGKVT KVQNWTDALF SLTVHAPVLP FTAGQFTKLG LEIDGERVQR
60 70 80 90 100
AYSYVNSPDN PDLEFYLVTV PDGKLSPRLA ALKPGDEVQV VSEAAGFFVL
110 120 130 140 150
DEVPHCETLW MLATGTAIGP YLSILQLGKD LDRFKNLVLV HAARYAADLS
160 170 180 190 200
YLPLMQELEK RYEGKLRIQT VVSRETAAGS LTGRIPALIE SGELESTIGL
210 220 230 240
PMNKETSHVM LCGNPQMVRD TQQLLKETRQ MTKHLRRRPG HMTAEHYW
Length:248
Mass (Da):27,751
Last modified:January 23, 2007 - v4
Checksum:iCBF46435A95709E4
GO

Sequence cautioni

The sequence Z11767 differs from that shown. Reason: Frameshift at positions 94 and 127.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04757 Genomic DNA. Translation: AAA23805.1.
L19201 Genomic DNA. Translation: AAB03056.1.
U00096 Genomic DNA. Translation: AAC76906.1.
AP009048 Genomic DNA. Translation: BAE77386.1.
Z11767 Genomic DNA. No translation available.
M19644 Genomic DNA. Translation: AAA24189.1. Sequence problems.
PIRiS40867.
RefSeqiNP_418359.1. NC_000913.3.
WP_000796332.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76906; AAC76906; b3924.
BAE77386; BAE77386; BAE77386.
GeneIDi948414.
KEGGiecj:JW3895.
eco:b3924.
PATRICi32123361. VBIEscCol129921_4041.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04757 Genomic DNA. Translation: AAA23805.1.
L19201 Genomic DNA. Translation: AAB03056.1.
U00096 Genomic DNA. Translation: AAC76906.1.
AP009048 Genomic DNA. Translation: BAE77386.1.
Z11767 Genomic DNA. No translation available.
M19644 Genomic DNA. Translation: AAA24189.1. Sequence problems.
PIRiS40867.
RefSeqiNP_418359.1. NC_000913.3.
WP_000796332.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FDRX-ray1.70A1-248[»]
2XNJX-ray1.90A/B2-247[»]
ProteinModelPortaliP28861.
SMRiP28861.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261115. 2 interactors.
IntActiP28861. 6 interactors.
STRINGi511145.b3924.

Chemistry databases

DrugBankiDB03147. Flavin adenine dinucleotide.

Proteomic databases

EPDiP28861.
PaxDbiP28861.
PRIDEiP28861.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76906; AAC76906; b3924.
BAE77386; BAE77386; BAE77386.
GeneIDi948414.
KEGGiecj:JW3895.
eco:b3924.
PATRICi32123361. VBIEscCol129921_4041.

Organism-specific databases

EchoBASEiEB1480.
EcoGeneiEG11518. fpr.

Phylogenomic databases

eggNOGiENOG4105DP0. Bacteria.
COG1018. LUCA.
HOGENOMiHOG000265758.
InParanoidiP28861.
KOiK00528.
OMAiPFIAGQF.
PhylomeDBiP28861.

Enzyme and pathway databases

BioCyciEcoCyc:FLAVONADPREDUCT-MONOMER.
ECOL316407:JW3895-MONOMER.
MetaCyc:FLAVONADPREDUCT-MONOMER.
BRENDAi1.18.1.2. 2026.

Miscellaneous databases

EvolutionaryTraceiP28861.
PROiP28861.

Family and domain databases

CDDicd06195. FNR1. 1 hit.
InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR033892. FNR_bac.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFENR_ECOLI
AccessioniPrimary (citable) accession number: P28861
Secondary accession number(s): P11007, Q2M8M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 142 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

PubMed:2834327 authors incorrectly assigned the protein to be part of FPR, the C-terminal of GlpX.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.